Volume 32, Issue 214 (11-2022)
J Mazandaran Univ Med Sci 2022, 32(214): 55-64 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Mirshafieyan N S, Hassanpourezatti M, dehghan H. Neuroprotective Effect of Nigella sativa Essential Oil against Experimental Demyelination through Regulation of Endogenous Fatty Acid Levels in Rat Spinal Cord Tissue. J Mazandaran Univ Med Sci 2022; 32 (214) :55-64
URL: http://jmums.mazums.ac.ir/article-1-18335-en.html
URL: http://jmums.mazums.ac.ir/article-1-18335-en.html
Abstract: (573 Views)
Background and purpose: Myelin degradation is one of the main causes of some neurological diseases. There is growing evidence that plant essential oils may reduce the risk of demyelination. In this experimental study, using a lipidomics approach, we investigated whether administration of Nigella sativa (NS) essential oil could improve changes in the profile of spinal fatty acids in demyelination model induced by intra-spinal administration of ethidium bromide (EB) in mice.
Materials and methods: In this experimental study, 30 male rats were studied in five groups (n=6). The groups included control without treatment, solvent, and black seed essential oil (2, 5, and 10 ml/kg, intraperitoneally). The essential oil of NS was prepared and its fatty acid composition was determined by gas chromatography-mass spectrometry (GC-MS). The animals underwent intra-spinal administration of ET 0.2%. At the end of the experiments, samples were taken from the spinal cord of the rats and the concentration of seven different types of fatty acids in the homogenate of the spinal cord was measured and compared between the groups.
Results: Ethidium bromide disturbed the normal concentration of polyunsaturated fatty acids, increased arachidonic acid derivatives, and decreased docosahexaenoic acid in rat spinal cord. Treatment with NS essential oil significantly prevented these changes in a dose-dependent manner (P<0.01).
Conclusion: NS essential oil protects against EB-induced demyelination by regulating the level of endogenous fatty acids in spinal tissue.
Materials and methods: In this experimental study, 30 male rats were studied in five groups (n=6). The groups included control without treatment, solvent, and black seed essential oil (2, 5, and 10 ml/kg, intraperitoneally). The essential oil of NS was prepared and its fatty acid composition was determined by gas chromatography-mass spectrometry (GC-MS). The animals underwent intra-spinal administration of ET 0.2%. At the end of the experiments, samples were taken from the spinal cord of the rats and the concentration of seven different types of fatty acids in the homogenate of the spinal cord was measured and compared between the groups.
Results: Ethidium bromide disturbed the normal concentration of polyunsaturated fatty acids, increased arachidonic acid derivatives, and decreased docosahexaenoic acid in rat spinal cord. Treatment with NS essential oil significantly prevented these changes in a dose-dependent manner (P<0.01).
Conclusion: NS essential oil protects against EB-induced demyelination by regulating the level of endogenous fatty acids in spinal tissue.
Type of Study: Research(Original) |
Subject:
physiology
Send email to the article author
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
Articles Copyright © The Author(s). Owned by Mazandaran University of Medical Sciences.
Contact Us
Address: Journal Office, Building No. 2, Mazandaran University of Medical Sciences, Moallem Square, Sari.Tel: +98 (011)34484874 Postal code: 48175-866 E-Mail: jmums@mazums.ac.ir Telefax: +98 (011)33262679
