Volume 24, Number 116 (9-2014)                   J Mazandaran Univ Med Sci 2014, 24(116): 141-148 | Back to browse issues page


XML Persian Abstract Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Khatami M, Eghbali Ebrahimabadi M. Molecular Analysis of tRNAGlu, Trp, Ala, Asp Genes of mtDNA in Patients with Long QT Syndrome. J Mazandaran Univ Med Sci. 2014; 24 (116) :141-148
URL: http://jmums.mazums.ac.ir/article-1-4240-en.html

Abstract:   (3328 Views)
Background and purpose: Long QT syndrome is a heart arrhythmia identified by prolongation of the QT interval which is a cause of sudden cardiac death in young individuals. In most cases, abnormalities in heart repolarization are reasons of prolongation of action potential and arrhythmia. The activity of ion channels is sensitive to ATP level, therefore, mitochondrial disorders are considered to be very important. The purpose of this study was to investigate the areas of mtDNA that are identified as hot spots in most cardiac diseases. Material and Methods: Total DNA was extracted from the peripheral blood of 30 patients with LQT syndrome and 35 normal persons. Mitochondrial tRNATrp-Ala-Apn-Glugenes and cytochrome b gene were amplified by polymerase chain reaction (PCR). Then, PCR fragments were screened for singlestrand conformation polymorphism analysis (SSCP) and all positive samples were sequenced. Results: We observed a homoplasmic and conserved T14687C mutation in the mitochondrial tRNA glutamic acid gene in one patient with pervious syncope. We found a homoplasmic C14766T transition in five patients that changed threonine to isoleucine substitution at amino acid 7. Also, we found a heteroplasmy G14838A mutation in mitochondrial cytochrome b gene which is nonsense and changed tryptophan to a stop codon in position 31. Conclusion: Mitochondria ATP synthesis is important in heart, therefore, it is possible that mutations in mitochondrial genes could constitute a predisposing factor in combination with environmental factors and may also trigger the syncope in patients with L QT.
Full-Text [PDF 358 kb]   (798 Downloads)    
Type of Study: Research(Original) | Subject: Biology

Add your comments about this article : Your username or email:
Write the security code in the box

Send email to the article author


© 2015 All Rights Reserved | Journal of Mazandaran University of Medical Sciences

Designed & Developed by : Yektaweb