Morphometrical study of mouse pre-implantation embryos: comparison of in vitro- and in vivo-produced embryos - Journal of Mazandaran University of Medical Sciences
Volume 18, Number 67 (Sep 2008)                   J Mazandaran Univ Med Sci 2008, 18(67): 43-49 | Back to browse issues page


XML Persian Abstract Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Karimpour Malekshah A, Bahrol-olumi S, Kalteh A, Esmailnejad Moghaddam A, Talebpour F. Morphometrical study of mouse pre-implantation embryos: comparison of in vitro- and in vivo-produced embryos. J Mazandaran Univ Med Sci. 2008; 18 (67) :43-49
URL: http://jmums.mazums.ac.ir/article-1-491-en.html

Abstract:   (7872 Views)
Background and purpose: The development of pre-implantation mammalian embryos in vitro is compromised, compared with those grown in vivo. Selecting embryos with a high implantation potential is one of the most important challenges in the field of assisted reproductive technology. The aim of this study was to postulate morphometrical characteristics of good quality embryos, with comparisons between in vivo and in vitro produced mouse embryos.
Materials and methods: Embryos was obtained from NMRI female mice after super ovulation. In vivo developed 2-, 4- and 8-cell embryos morulla and full blastocyst were isolated from mice on 18, 36, 52, 60, 72 and 96 hours after hCG administration respectively. Ham, s F10 medium was used for in vitro culture of embryos. External and internal diameter of embryos, zona thickness and number of cells in full blastocysts were evaluated and compared between in vivo and in vitro groups.
Results: External and internal diameter and zone thickness in oocyte and zygotes were 99.9µm, 75.4µm and 4.9µm respectively. These values did not change prior to the blastocyst stage in both in vivo and in vitro groups but in full blastocyst stage, the diameter of embryos significantly increased and zone thickness decreased compared to prior stages in both groups (P<0.01). The diameter of full blastocysts of in vivo group (116.5 µm) were significantly larger than those of in vitro group (104.3 µm, P<0.05). Moreover, the full blastocysts of in vivo group had significantly more blastomeres (49), compared to in vitro group (43, P<0.05). Additionally, cultured embryos reached full blastocyst at 110 hours after hCG administration, while in vivo condition the time frame was 96 hours.
Conclusion: Based on the above results, embryo size and zona thickness can not predict embryo quality prior to blastocyst stage, however, in this stage larger embryos and those that have more blastomere may show greater viability.
Full-Text [PDF 222 kb]   (1326 Downloads)    
Type of Study: Research(Original) |

Add your comments about this article : Your username or email:
Write the security code in the box

Send email to the article author


© 2015 All Rights Reserved | Journal of Mazandaran University of Medical Sciences

Designed & Developed by : Yektaweb