Volume 26, Issue 135 (4-2016)                   J Mazandaran Univ Med Sci 2016, 26(135): 32-42 | Back to browse issues page

XML Persian Abstract Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Khajehzadeh S, Behbahani M. Activity of Avicennia marina Methanol Extracts on Proliferation of Lymphocytes and their Mutagenicity Using Ames Test and In Silico Method. J Mazandaran Univ Med Sci 2016; 26 (135) :32-42
URL: http://jmums.mazums.ac.ir/article-1-7332-en.html
Abstract:   (6387 Views)

Background and purpose: Avicennia marina (family Acanthaceae) has been used as traditional medicine in Iran to treat some diseases such as ulcers, rheumatism and burns. The present study investigated the in silico and in vitro mutagenicity of the fruit, leaf, seed and stem extracts of Avicennia marina and their effects on human peripheral blood mononuclear cells (PBMC) proliferation.

Materials and methods: In this experimental study, air dried and powdered plant materials were extracted by methanol using maceration. The extracts were evaporated to dryness by rotary evaporator at 40°C and were diluted using different PBS concentrations (50, 100, 500, 1000, and 1500 µg/ml). The effect of the methanol extract of this plant on lymphocyte proliferation was measured using MTT assay. The mutagenicity of these extracts was also investigated using Ames test. In silico analysis of 15 dominant compounds of the plant was performed by Toxtree 2.6.6 software.

Results: The methanol extracts of the leaf and root had the highest and lowest inducing effect on lymphocyte proliferation, respectively. Leaf and stem extracts of Avicennia marina did not show any mutagenicity on this strain. In silico analysis demonstrated that among 15 compounds, four triterpenoids (Taraxerol, Betulin, Lupeol, and Gossypol) had weak mutagenicity.

Conclusion: Avicennia marina showed positive effects on proliferation of lymphocytes and their mutagenicity, therefore, it could be considered as a good candidate in treatment of immunodeficiency diseases.

Full-Text [PDF 719 kb]   (1823 Downloads)    
Type of Study: Research(Original) |

Add your comments about this article : Your username or Email:
CAPTCHA

Send email to the article author


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2024 CC BY-NC 4.0 | Journal of Mazandaran University of Medical Sciences

Designed & Developed by : Yektaweb