Volume 13, Issue 40 (Sep 2003)
J Mazandaran Univ Med Sci 2003, 13(40): 1-8 |
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Abstract: (13292 Views)
Background and purpose: Since histologists studied suprarenal glands more with immuno histochemical methods, and less about its surface cell glycoconjugate chains. Therefore this study was conducted to examine sugar or terminal glycoconjugate sugar chains of surface developing medullary chromatinic cells in mouse embryonic suprarenal glands.
Materials and methods: Mice embryos at days 13th to 20th of gestation developed by mating of 26 balb/c mice, were desected in diameter of 5 micrometers by serial section method after tissue processing. For observation of suprarenal glands and selection of proper sample, specimens were first stained with hematoxyline eosine (H&Ë) methods, then by using lectin, histochemistry processing microssopic study was done.
Results: Âmong the used lectins such as, MPÂ, PNÂ, VVÂ, WGÂ and ÔFÂ, only MPÂ on different embryonic days, with intensity and weakness related to the age, increase and/or decrease of chromaffinic cells, showed medullary region of adrenal gland. PNÂ was used as positive control.
Çonclusion: Since only MPÂ lectin on the days of embryonic study had reaction with the chromaffin cells of suprarenal gland medullary region, and specifically binds to the cell surface receptor Gal-GalNÂc (Galactose-N-Âcetyl Galactosamine). Therefore it is proposed that, termal sugar Gal-GalNÂc as a recipient of the development inducing factors, be considered an important factor in development of chromaffin cells at medullary region of suprarenal gland.
Materials and methods: Mice embryos at days 13th to 20th of gestation developed by mating of 26 balb/c mice, were desected in diameter of 5 micrometers by serial section method after tissue processing. For observation of suprarenal glands and selection of proper sample, specimens were first stained with hematoxyline eosine (H&Ë) methods, then by using lectin, histochemistry processing microssopic study was done.
Results: Âmong the used lectins such as, MPÂ, PNÂ, VVÂ, WGÂ and ÔFÂ, only MPÂ on different embryonic days, with intensity and weakness related to the age, increase and/or decrease of chromaffinic cells, showed medullary region of adrenal gland. PNÂ was used as positive control.
Çonclusion: Since only MPÂ lectin on the days of embryonic study had reaction with the chromaffin cells of suprarenal gland medullary region, and specifically binds to the cell surface receptor Gal-GalNÂc (Galactose-N-Âcetyl Galactosamine). Therefore it is proposed that, termal sugar Gal-GalNÂc as a recipient of the development inducing factors, be considered an important factor in development of chromaffin cells at medullary region of suprarenal gland.
Type of Study: Research(Original) |
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