TY - JOUR T1 - Frequency Evaluation of C3435T Polymorphism of the MDR1 Gene in a Healthy Population in Mazandaran Province TT - بررسی فراوانی پلی مورفیسم ژنتیکی C3435T ژن MDR1 در یک جمعیت سالم استان مازندران JF - J-Mazand-Univ-Med-Sci JO - J-Mazand-Univ-Med-Sci VL - 23 IS - 107 UR - http://jmums.mazums.ac.ir/article-1-2998-en.html Y1 - 2013 SP - 2 EP - 10 KW - P–glycoprotein KW - MDR1 gene KW - Pharmacokinetics KW - C3435T polymorphism N2 - Background and purpose: The human multidrug resistance gene (MDR1) encodes for P-glycoprotein (P-gp) which is a transmembrane transporter protein acts as an efflux pump for a number of xenobiotics. It plays a protective role for cells against DNA damage caused by toxins and drugs. The wobble C3435T polymorphism at exon 26 has been associated with different expression levels and activities of this gene. Differences in allele frequency of the C3435T polymorphism have been demonstrated between distinct ethnic groups. In this study, we examined this polymorphism in a healthy population in Mazandaran province (North of Iran). Material and methods: Genomic DNA was extracted from the peripheral leukocytes of 150 healthy volunteers under the supervision of two physicians in Sari Blood Transfusion Center. All subjects gave their written informed consent to participate in the research. Polymerase chain reaction–restriction fragment length polymorphism method was used for the detection of C3435T single nucleotide polymorphism and the results were confirmed by direct sequencing method. Results: The frequencies of the CC, CT and TT genotypes were 10.7 %, 64 % and 25.3%, respectively. The frequency of T allele (57.3%) was significantly higher than C allele (42.7 %) (P M3 ER -