@ARTICLE{Hosseini, author = {Hosseini, Asieh and Sharifi, Zohreh and Baghbani-arani, Fahimeh and }, title = {Survey on the Association of CCR5 Promoter -59353T/C Polymorphism with HCV Infection}, volume = {23}, number = {109}, abstract ={Background and purpose: Hepatitis C is an acute or chronic liver disease worldwide. Chemokines and chemokine receptors are involved in the effective immune response to hepatitis C virus (HCV) infection through the efficient recruitment and activation of inflammatory cells to infected liver. The CC-chemokine receptor (CCR) 5 is expressed on the several cells of the immune system and has been reported that the CCR5-59353T/C polymorphism may be associated with HCV infection. Hence, this study was aimed to determine the association of this polymorphism with susceptibility to HCV infection in Iranian patients. Material and methods: In this study, 100 HCV infected patients and 100 healthy individuals were randomly selected. Genomic DNA was extracted from blood buffy coat using the salting out method. The CCR5-59353(T/C) genotypes were determined using Allele Specific PCR method. The PCR products were electrophoresed on 1.5% agarose gel. The data were analyzed using Chi-square test. Results: The frequency of C variant allele was 42% in HCV infected patients and 37% in healthy individuals which was not statistically significant (P= 0.3). Also, the frequency of CCR5-59353C/C genotype was 16% in patients infected with HCV and 12% in healthy individuals and no significant difference was observed between the two groups (P= 0.4). Conclusion: Based on our findings, CCR5-593535T/C polymorphism is not effective in the study population as a host genetic factor in determining susceptibility to HCV infection. }, URL = {http://jmums.mazums.ac.ir/article-1-3378-en.html}, eprint = {http://jmums.mazums.ac.ir/article-1-3378-en.pdf}, journal = {Journal of Mazandaran University of Medical Sciences}, doi = {}, year = {2014} }