TY - JOUR T1 - Preparation and Radiochemical Evaluation of 99mTc-HYNIC-[Lys3,Tyr4] bombesin (3-14) for Prostate Cancer Detection TT - آماده سازی و ارزیابی رادیوشیمیایی HYNIC-[ Lys3,Tyr4]bombesin (3-14) نشاندار شده با تکنسیوم- m99 برای ردیابی سرطان پروستات JF - J-Mazand-Univ-Med-Sci JO - J-Mazand-Univ-Med-Sci VL - 26 IS - 144 UR - http://jmums.mazums.ac.ir/article-1-9284-en.html Y1 - 2017 SP - 367 EP - 372 KW - bombesin KW - prostate cancer KW - 99mTc KW - gastrin releasing peptide N2 - Background and purpose: Over-expression of GRP receptors for Bombesin has been reported to be found in malignant tissues, especially in prostate cancer. Here, we aimed at radiochemical and stability evaluation of a new bombesin derivative labeled with 99mTc via HYNIC as a chelating agent and Tricine/EDDA as coligands that might be used for diagnosis of prostate cancer. Materials and methods: In this experimental study (performed in 2016), the prepared bombesin was added to aqueous solution of Tricine/EDDA, then we added SnCl2, which was dissolved in HCl-containing solution. Finally the prepared bombesin was treated in 95 °C for 10 minutes. We investigated raiochemical purity using TLC and HPLC methods. Also, its stability in normal saline and human serum and percentage of protein-binding were studied. Results: Radiochemical purity was more than 98%. Stability in normal salin was 97.5, 96, and 95.5% after 1, 4, and 24 hours, respectively. Stability in human serum after 1 hour was more than 98% and after 4 hours was more than 92%. Percentage of protein-binding was 12.5, 14, and 16%, after 1, 4, and 24 hours, respectively. Conclusion: This new bombesin derivative was easily labeled with 99mTc using exchange labeling and has some promising characteristics for prostate cancer detection. M3 ER -