RT - Journal Article T1 - Cloning, Expression, and Purification of Recombinant Beta Subunit of Human TSH Protein and Evaluating its Antigenicity JF - J-Mazand-Univ-Med-Sci YR - 2017 JO - J-Mazand-Univ-Med-Sci VO - 27 IS - 147 UR - http://jmums.mazums.ac.ir/article-1-9812-en.html SP - 87 EP - 96 K1 - ELISA K1 - molecular cloning K1 - recombinant protein K1 - thyroid stimulating hormone K1 - thyrotropin beta subunit AB - Background and purpose: Measurement of thyroid stimulating hormone (TSH), usually through RIA and ELISA tests, is very useful for the diagnosis of thyroid disorders. Considering the structural similarity between alpha chain of TSH and the three glycoprotein hormones of luteinizing hormone, follicle stimulating hormone, and chorionic gonadotropin (CG), in this study, we aimed to examine the produced beta-subunit of TSH (TSHβ) in terms of antigenicity. Materials and methods: In this study, human TSHβ gene was synthesized using recombinant method. The cloning, expression, and purification were performed in Escherichia coli. Thereafter, antigenicity of the protein against antibodies against animal monoclonal TSH was evaluated using Western Blot technique. Results: TSHβ gene was cloned and expressed correctly in the bacterial host. The purified protein reacted with the specific antibody in Western Blot. Conclusion: Although recombinant TSHβ protein is different from the natural protein in some respects such as structure and spatial shape, it had acceptable antigenicity. On the other hand, it seems that this protein, due to having more TSH epitopes, can be applied in diagnostic kits to enahance the specificity of these tests. LA eng UL http://jmums.mazums.ac.ir/article-1-9812-en.html M3 ER -