TY - JOUR T1 - In vitro/In vivo Study of a New Bombesin Derivative with Tripeptide (Gly-Gly-Gly) Spacer as Potential Targeted Tumor Imaging Agent TT - مطالعه درون تنی و برون تنی مشتق جدیدی از بومبزین با فاصله دهنده تری گلایسین به عنوان عامل بالقوه در تصویربرداری هدفمند تومور JF - J-Mazand-Univ-Med-Sci JO - J-Mazand-Univ-Med-Sci VL - 26 IS - 140 UR - http://jmums.mazums.ac.ir/article-1-8372-en.html Y1 - 2016 SP - 15 EP - 23 KW - Bombesin KW - 99mTc KW - GRP receptors KW - tumor KW - imaging N2 - Background and purpose: Bombesin is a 14-amino acid peptide with high biological functions which has a high affinity to GRP )Gastrin releasing peptide( receptor. GRP receptors are overexpressed in many malignant tumors such as prostate and breast cancers. The purpose of this study was to prepare [HYNIC-(Gly)3-D-Phe13]Bombesin(7-14) to label it easily by 99mTc and to evaluate it as a potential agent for imaging the tumors that have GRP receptors. Materials and methods: In this experimental study, the prepared derivative Bombesin was radiolabelled by 99mTc at 100°C in 10 minutes using the exchange method and radiolabelling analysis was applied by TLC and HPLC. Specific absorption test and specific internalization using prostate cancer cells PC-3 was done by blocking and unblocking the receptors. Biodistribution were determined in normal mice. Results: More than 95% radiolabelling was obtained. Specific absorption and internalization after 4 hours were 17.9% and 9.5%, respectively in PC-3 cancer cells. Biodistribution data showed rapid clearance from blood with major renal excretion and specific binding to tissues that have GRP receptors such as pancreas. Conclusion: Conjugated peptide radiolabelled with 99mTc has promising characteristics as potential agent for imaging the tumors that overexpress the GRP receptors. M3 ER -