RT - Journal Article T1 - Prevalence of Human Papillomavirus Genotypes in Cervical Cancer Specimens in Tehran JF - J-Mazand-Univ-Med-Sci YR - 2015 JO - J-Mazand-Univ-Med-Sci VO - 25 IS - 123 UR - http://jmums.mazums.ac.ir/article-1-5529-en.html SP - 185 EP - 190 K1 - Human papillomavirus K1 - genotype K1 - cervical cancer AB - Background and purpose: Human papillomavirus infection is the most important risk factor and necessary cause of cervical cancer. The aim of this study was to determine the genotypes of HPV DNA in cervical carcinoma samples from Shohada and Imam Hossien Hospitals in Tehran, between 1999 and 2008. Materials and methods: After the paraffin alleviation process from the sections needed for HPV typing, the DNA was extracted from the genome virus. Then, PCR techniques were applied for identification and replication of the HPV virus genome from the tissues. Afterwards, the samples were placed into an incubator operating at a proper temperature and 28 genetic sequences for HPV genotypes were detected using Reverse Dot Blot Method. Results: There were 58 cases, of which 44 were HPV positive (75.9%) and the remaining samples were negative for HPV virus (24.1%). Among different genotypes of HPV, types 18 and 16 were found to be more frequent (13.7% and 62%, respectively). Also, there were 48 cases of squamous cell carcinoma of which 35 (80%) were HPV type 16. Conclusion: High prevalence of HPV in Iranian women calls for efficient health promotion strategies, more focus on appropriate sexual behavior, and prevention of sexually transmitted diseases. These measures are great steps in preventing infection with HPV and cervical cancer. LA eng UL http://jmums.mazums.ac.ir/article-1-5529-en.html M3 ER -