TY - JOUR T1 - Comparing the Impact of Different Extraction Methods on Antioxidant Activities of Myrtle (Myrtus communis L.) TT - مقایسه اهمیت روش های مختلف استخراج بر فعالیت آنتی اکسیدانی برگ گیاه مورد Myrtus communis JF - J-Mazand-Univ-Med-Sci JO - J-Mazand-Univ-Med-Sci VL - 25 IS - 127 UR - http://jmums.mazums.ac.ir/article-1-6073-en.html Y1 - 2015 SP - 10 EP - 24 KW - Myrtle KW - Myrtus communis KW - extraction methods KW - antioxidant KW - total phenolics KW - total flavonoids N2 - Background and purpose: Myrtus communis L. (Myrtle, Myrtaceae) is a known medicinal plant used in traditional medicine worldwide. It is a well-known plant with distinctive antioxidant activity. In this study we investigated the impact of extraction methods on total phenolic and flavonoids contents and antioxidant activities of Myrtle (Myrtus communis L.) leaf. Materials and methods: The shade-dried leaf was extracted by three different methods including meceration, ultrasonic assisted, and soxhlet assisted extraction. Antioxidant capacity was assessed using four different methods: DPPH and nitric oxide (NO) free radicals scavenging, reducing power and iron chelating activity. The total phenolic and flavonoid contents were also identified. The data were analyzed by analysis of variance and the means separated by Newman-Keuls Multiple Comparison test (GraphPad Prism 5). Results: The highest yield of extraction was achieved with soxhlet assisted extraction. It also showed highest amount of total phenolics and flavonoids contents. In DPPH radical scavenging activity, the soxhlet extract (IC50 = 11.3 ± 0.3 µg ml) had a higher activity which was significantly different from other extracts (P< 0.05). All extracts were stronger than BHA (P M3 ER -