TY - JOUR T1 - Expression of Oncoprotein Gankyrin in Drug Resistant and Sensitive Cancer Ovarian and Gastric Cancer Cell Lines TT - بررسی میزان بیان اونکوپروتئین گانکیرین در رده های سلولی سرطان تخمدان و معده مقاوم و حساس به داروهای ضد سرطان JF - J-Mazand-Univ-Med-Sci JO - J-Mazand-Univ-Med-Sci VL - 28 IS - 161 UR - http://jmums.mazums.ac.ir/article-1-10158-en.html Y1 - 2018 SP - 12 EP - 23 KW - gankyrin KW - multidrug resistance KW - ovarian cancer KW - gastric cancer N2 - Background and purpose: Multidrug resistance (MDR) phenotype is a major complication in cancer chemotherapy. Gankyrin is a key point molecule in cell cycle regulation and may contribute to drug-resistance phenotype of tumor cells. The aim of this study was to compare the basal Gankyrin expression level in ovarian and gastric drug-resistant cells with their parental drug-sensitive cells. Materials and methods: In this experimental study, the Gankyrin expression at mRNA and protein levels in drug resistance cell lines was compared with their parental drug sensitive cell lines by real time RT-PCR and Western blotting. Results: Gankyrin mRNA expression in A2780/RCIS cells was statistically lower than A2780 cells (p= 0.00) but their difference was not statistically significant at protein level (p= 0.240). In addition, Gankyrin expression in EPG85-257/RDB was lower than its parental drug sensitive cell line at mRNA and protein level. Conclusion: This study found an opposite relationship between Gankyrin expression and MDR phenotype related to the overexpression of MDR transportes. This suggestes for the first time that the incidence of MDR phenotype could inhibit tumorgenisity. M3 ER -