Journal of Mazandaran University of Medical Sciences
مجله دانشگاه علوم پزشکی مازندران
J Mazandaran Univ Med Sci
Medical Sciences
http://jmums.mazums.ac.ir
1
admin
1735-9260
1735-9279
fa
jalali
1399
8
1
gregorian
2020
11
1
30
190
online
1
fulltext
fa
بررسی اثر ماده موثره (EGCG) epigallocatechin-gallate بر تولید سلول های دندریتیک از مونوسیت های خون محیطی انسانی
Effect of Epigallocatechin gallate (EGCG) on Production of Dendritic Cells from Peripheral Blood Monocytes
ايمونولوژي
Immunology
پژوهشي-کامل
Research(Original)
<span style="letter-spacing:-.1pt;"><span style="color:black;"><span style="font-family:B Koodak;">سابقه</span></span></span><span style="letter-spacing:-.1pt;"><span style="color:black;"><span style="font-family:B Koodak;"> و هدف:</span></span></span> <span style="color:black;"><span style="font-family:B Zar;">سلول</span></span><span style="layout-grid-mode:line;"><span style="font-family:B Zar;"></span></span><span style="color:black;"><span style="font-family:B Zar;">های دندریتیک، سلول</span></span><span style="layout-grid-mode:line;"><span style="font-family:B Zar;"></span></span><span style="color:black;"><span style="font-family:B Zar;">های حرفه</span></span><span style="layout-grid-mode:line;"><span style="font-family:B Zar;"></span></span><span style="color:black;"><span style="font-family:B Zar;">ای عرضه</span></span><span style="layout-grid-mode:line;"><span style="font-family:B Zar;"></span></span><span style="color:black;"><span style="font-family:B Zar;">کننده آنتی</span></span><span style="layout-grid-mode:line;"><span style="font-family:B Zar;"></span></span><span style="color:black;"><span style="font-family:B Zar;">ژن هستند که آغازگر و تعدیل</span></span><span style="layout-grid-mode:line;"><span style="font-family:B Zar;"></span></span><span style="color:black;"><span style="font-family:B Zar;">کننده پاسخ</span></span><span style="layout-grid-mode:line;"><span style="font-family:B Zar;"></span></span><span style="color:black;"><span style="font-family:B Zar;">های ایمنی می</span></span><span style="layout-grid-mode:line;"><span style="font-family:B Zar;"></span></span><span style="color:black;"><span style="font-family:B Zar;">باشند. ماده </span></span><span style="color:black;"><span style="font-family:B Zar;">شیمیایی</span></span> <span dir="LTR"><span style="color:black;"><span style="font-size:10.0pt;">epigallocatechin gallate</span></span></span> <span style="color:black;"><span style="font-family:B Zar;">(</span></span><span dir="LTR"><span style="color:black;"><span style="font-size:10.0pt;">EGCG</span></span></span><span style="color:black;"><span style="font-family:B Zar;">) چای سبز یکی از عوامل</span></span> <span style="color:black;"><span style="font-family:B Zar;">سرکوبگر تومور شناخته شده است. از آن</span></span><span style="layout-grid-mode:line;"><span style="font-family:B Zar;"></span></span><span style="color:black;"><span style="font-family:B Zar;">جایی که اینترلوکین4 (</span></span><span dir="LTR"><span style="color:black;"><span style="font-size:10.0pt;">IL-4</span></span></span><span style="color:black;"><span style="font-family:B Zar;">) تولید سایتوکاین</span></span><span style="layout-grid-mode:line;"><span style="font-family:B Zar;"></span></span><span style="color:black;"><span style="font-family:B Zar;">های پیش التهابی را مهار می</span></span><span style="layout-grid-mode:line;"><span style="font-family:B Zar;"></span></span><span style="color:black;"><span style="font-family:B Zar;">کند</span></span> <span style="color:black;"><span style="font-family:B Zar;">و </span></span><span dir="LTR"><span style="color:black;"><span style="font-size:10.0pt;">EGCG</span></span></span><span style="color:black;"><span style="font-family:B Zar;"> نیز دارای اثر ضد التهابی و تعدیل</span></span><span style="layout-grid-mode:line;"><span style="font-family:B Zar;"></span></span><span style="color:black;"><span style="font-family:B Zar;">کننده ایمنی می</span></span><span style="layout-grid-mode:line;"><span style="font-family:B Zar;"></span></span><span style="color:black;"><span style="font-family:B Zar;">باشد، لذا هدف از این مطالعه</span></span><span style="color:black;"><span style="font-family:B Zar;">،</span></span><span style="color:black;"><span style="font-family:B Zar;"> بررسی اثر </span></span><span dir="LTR"><span style="color:black;"><span style="font-size:10.0pt;">EGCG</span></span></span><span style="color:black;"><span style="font-family:B Zar;"> در تمایز مونوسیت</span></span><span style="layout-grid-mode:line;"><span style="font-family:B Zar;"></span></span><span style="color:black;"><span style="font-family:B Zar;">ها به سلول</span></span><span style="layout-grid-mode:line;"><span style="font-family:B Zar;"></span></span><span style="color:black;"><span style="font-family:B Zar;">های دندریتیک به عنوان جایگزینی مناسب برای </span></span><span dir="LTR"><span style="color:black;"><span style="font-size:10.0pt;">IL-4</span></span></span><span style="color:black;"><span style="font-family:B Zar;"> بوده است .</span></span><span dir="LTR"><span style="color:black;"><span style="font-size:10.0pt;"></span></span></span><br>
<span style="letter-spacing:-.1pt;"><span style="color:black;"><span style="font-family:B Koodak;">مواد و روش</span></span></span><span style="letter-spacing:-.1pt;"><span style="color:black;"><span style="font-family:B Zar;"></span></span></span><span style="letter-spacing:-.1pt;"><span style="color:black;"><span style="font-family:B Koodak;">ها:</span></span></span> <span style="color:black;"><span style="font-family:B Zar;">در این مطالعه تجربی، سلول</span></span><span style="layout-grid-mode:line;"><span style="font-family:B Zar;"></span></span><span style="color:black;"><span style="font-family:B Zar;">های تک هسته</span></span><span style="layout-grid-mode:line;"><span style="font-family:B Zar;"></span></span><span style="color:black;"><span style="font-family:B Zar;">ای خون محیطی با استفاده از فایکول جداسازی و سلول</span></span><span style="layout-grid-mode:line;"><span style="font-family:B Zar;"></span></span><span style="color:black;"><span style="font-family:B Zar;">های مونوسیت </span></span><span dir="LTR"><span style="color:black;"><span style="font-size:10.0pt;">CD14<sup>+</sup></span></span></span><span style="color:black;"><span style="font-family:B Zar;"> به روش </span></span><span dir="LTR"><span style="color:black;"><span style="font-size:10.0pt;">MACS</span></span></span><span style="color:black;"><span style="font-family:B Zar;"> جمع</span></span><span style="layout-grid-mode:line;"><span style="font-family:B Zar;"></span></span><span style="color:black;"><span style="font-family:B Zar;">آوری شدند. </span></span><span dir="LTR"><span style="color:black;"><span style="font-size:10.0pt;">EGCG</span></span></span><span style="color:black;"><span style="font-family:B Zar;"> در دو غلظت </span></span><span dir="LTR"><span style="color:black;"><span style="font-size:10.0pt;">µmol/l</span></span></span><span style="color:black;"><span style="font-family:B Zar;"> 5 و </span></span><span dir="LTR"><span style="color:black;"><span style="font-size:10.0pt;">µmol<strong>/l</strong></span></span></span><span style="color:black;"><span style="font-family:B Zar;"> 10 اضافه شد. سلول</span></span><span style="layout-grid-mode:line;"><span style="font-family:B Zar;"></span></span><span style="color:black;"><span style="font-family:B Zar;">ها درروز ششم و هشتم از جهت شاخص</span></span><span style="layout-grid-mode:line;"><span style="font-family:B Zar;"></span></span><span style="color:black;"><span style="font-family:B Zar;">های تمایزی </span></span><span dir="LTR"><span style="color:black;"><span style="font-size:10.0pt;">CD83</span></span></span><span style="color:black;"><span style="font-family:B Zar;">، </span></span><span dir="LTR"><span style="color:black;"><span style="font-size:10.0pt;">HLA-DR</span></span></span> <span style="color:black;"><span style="font-family:B Zar;">و </span></span><span dir="LTR"><span style="color:black;"><span style="font-size:10.0pt;"> CD11b</span></span></span><span style="color:black;"><span style="font-family:B Zar;">به روش فلوسیتومتری ارزیابی شدند. </span></span><span dir="LTR"><span style="color:black;"><span style="font-size:10.0pt;">IL-23</span></span></span><span style="color:black;"><span style="font-family:B Zar;"> و </span></span><span dir="LTR"><span style="color:black;"><span style="font-size:10.0pt;">IL-35</span></span></span><span style="color:black;"><span style="font-family:B Zar;"> نیز در مایع رویی کشت سلولی بررسی شدند.</span></span><br>
<span style="color:black;"><span style="font-family:B Koodak;">یافته</span></span><span style="color:black;"><span style="font-family:B Zar;"></span></span><span style="color:black;"><span style="font-family:B Koodak;">ها:</span></span><span style="color:black;"><span style="font-family:B Zar;"> مولکول</span></span><span style="layout-grid-mode:line;"><span style="font-family:B Zar;"></span></span><span style="color:black;"><span style="font-family:B Zar;">های </span></span><span dir="LTR"><span style="color:black;"><span style="font-size:10.0pt;">CD11b</span></span></span><span style="color:black;"><span style="font-family:B Zar;"> و </span></span><span dir="LTR"><span style="color:black;"><span style="font-size:10.0pt;">CD83</span></span></span><span style="color:black;"><span style="font-family:B Zar;"> در سلول</span></span><span style="layout-grid-mode:line;"><span style="font-family:B Zar;"></span></span><span style="color:black;"><span style="font-family:B Zar;">های دندریتیک بالغ و نابالغ، تفاوت معنی</span></span><span style="layout-grid-mode:line;"><span style="font-family:B Zar;"></span></span><span style="color:black;"><span style="font-family:B Zar;">داری بین دو روش داشت (05/0</span></span><span dir="LTR"><span style="color:black;"><span style="font-size:10.0pt;"><</span></span></span> <span dir="LTR"><span style="color:black;"><span style="font-size:10.0pt;">P</span></span></span><span style="color:black;"><span style="font-family:B Zar;">) </span></span><span style="color:black;"><span style="font-family:B Zar;">مولکول </span></span><span dir="LTR"><span style="color:black;"><span style="font-size:10.0pt;">HLA-DR</span></span></span><span style="color:black;"><span style="font-family:B Zar;"> در سلول</span></span><span style="layout-grid-mode:line;"><span style="font-family:B Zar;"></span></span><span style="color:black;"><span style="font-family:B Zar;">های دندریتیک نیز تفاوت معنی</span></span><span style="layout-grid-mode:line;"><span style="font-family:B Zar;"></span></span><span style="color:black;"><span style="font-family:B Zar;">داری داشت(05/0</span></span><span dir="LTR"><span style="color:black;"><span style="font-size:10.0pt;"><</span></span></span> <span dir="LTR"><span style="color:black;"><span style="font-size:10.0pt;">P</span></span></span><span style="color:black;"><span style="font-family:B Zar;">). </span></span><span dir="LTR"><span style="color:black;"><span style="font-size:10.0pt;">IL-23</span></span></span><span style="color:black;"><span style="font-family:B Zar;"> و </span></span><span dir="LTR"><span style="color:black;"><span style="font-size:10.0pt;">IL-35</span></span></span><span style="color:black;"><span style="font-family:B Zar;"> در مرحله نابالغ نسبت به سلول</span></span><span style="layout-grid-mode:line;"><span style="font-family:B Zar;"></span></span><span style="color:black;"><span style="font-family:B Zar;">های دندریتیک بالغ تفاوت معنی</span></span><span style="layout-grid-mode:line;"><span style="font-family:B Zar;"></span></span><span style="color:black;"><span style="font-family:B Zar;">داری نداشتند ولی در مرحله بالغ بین دو روش تفاوت معنی</span></span><span style="layout-grid-mode:line;"><span style="font-family:B Zar;"></span></span><span style="color:black;"><span style="font-family:B Zar;">داری دیده شد (05/0</span></span><span dir="LTR"><span style="color:black;"><span style="font-size:10.0pt;"><</span></span></span> <span dir="LTR"><span style="color:black;"><span style="font-size:10.0pt;">P</span></span></span><span style="color:black;"><span style="font-family:B Zar;">).</span></span><br>
<span style="letter-spacing:-.1pt;"><span style="color:black;"><span style="font-family:B Koodak;">استنتاج:</span></span></span> <span style="letter-spacing:-.1pt;"><span style="color:black;"><span style="font-family:B Zar;">بر اساس نتایج این مطالعه، </span></span></span><span dir="LTR" style="letter-spacing:-.1pt;"><span style="color:black;"><span style="font-size:10.0pt;">EGCG</span></span></span> <span style="letter-spacing:-.1pt;"><span style="color:black;"><span style="font-family:B Zar;">درمقایسه با </span></span></span><span dir="LTR" style="letter-spacing:-.1pt;"><span style="color:black;"><span style="font-size:10.0pt;">IL-4</span></span></span> <span style="letter-spacing:-.1pt;"><span style="color:black;"><span style="font-family:B Zar;">توانایی تمایز مونوسیت</span></span></span><span style="layout-grid-mode:line;"><span style="font-family:B Zar;"></span></span><span style="letter-spacing:-.1pt;"><span style="color:black;"><span style="font-family:B Zar;">ها به سلول</span></span></span><span style="layout-grid-mode:line;"><span style="font-family:B Zar;"></span></span><span style="letter-spacing:-.1pt;"><span style="color:black;"><span style="font-family:B Zar;">های دندریتیک نابالغ را دارد و به</span></span></span><span style="letter-spacing:-.1pt;layout-grid-mode:line;"><span style="font-family:B Zar;"></span></span><span style="letter-spacing:-.1pt;"><span style="color:black;"><span style="font-family:B Zar;">نظر می</span></span></span><span style="letter-spacing:-.1pt;layout-grid-mode:line;"><span style="font-family:B Zar;"></span></span><span style="letter-spacing:-.1pt;"><span style="color:black;"><span style="font-family:B Zar;">رسد این مولکول باعث تولید سلول</span></span></span><span style="letter-spacing:-.1pt;layout-grid-mode:line;"><span style="font-family:B Zar;"></span></span><span style="letter-spacing:-.1pt;"><span style="color:black;"><span style="font-family:B Zar;">هایی دندریتیک با خاصیت تولروژنیک نسبت به روش کلاسیک می</span></span></span><span style="letter-spacing:-.1pt;layout-grid-mode:line;"><span style="font-family:B Zar;"></span></span><span style="letter-spacing:-.1pt;"><span style="color:black;"><span style="font-family:B Zar;">شود</span></span></span><span style="letter-spacing:-.1pt;"><span style="color:black;">.</span></span><span style="letter-spacing:-.1pt;"><span style="color:black;"><span style="font-family:B Zar;"></span></span></span><br>
<strong>Background and purpose:</strong> Dendritic cells are professional antigen presenting cells that initiate and modulate immune responses. Epigallocatechin gallate (EGCG) is identified as a prophylactic agent that can suppress tumor formation. This research aimed at investigating the effect of EGCG on differentiation of dendritic cells from monocytes and as a potential substitute for IL-4 in this process.<br>
<strong>Materials and methods:</strong> In this experimental study, blood samples were drawn and peripheral blood mononuclear cells were isolated using FICOL. The monocytes CD14 + were purified by MACS. EGCG was added in 5 μmol / l and 10 μmol / l. On days six and eight, the cells were analyzed and differentiation factors such as CD83, HLA-DR, and CD-b were evaluated using flow cytometry. In addition, IL-23 and IL-35 levels were measured.<br>
<strong>Results:</strong> CD-11 and CD11b levels in mature and immature dendritic cells were significantly different between the two paths (P<0.05), HLA-DR levels were also significantly different in dendritic cells (P<0.05). IL-23 and IL-35 levels in mature and immature dendritic cells were not significantly different, whereas these levels in mature dendritic cells showed significant differences between the two paths (P<0.05).<br>
<strong>Conclusion:</strong> This study showed that EGCG compared to IL-4 can initiate monocyte differentiation to dendritic cells and this molecule can turn monocytes to dendritic cells with tolerogenic qualities compared to the classic path.
EGCG, اینترلوکین 35, اینترلوکین 23
EGCG, IL-35, IL-23
1
10
http://jmums.mazums.ac.ir/browse.php?a_code=A-10-258-12&slc_lang=fa&sid=1
Mohammad Ali
Mofid Nakhaei
محمد علی
مفید نخعی
nakhaeilab@yahoo.com
1003194753284600124902
1003194753284600124902
No
MSc Student in Immunology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
دانشجوی کارشناسی ارشد ایمونولوژی، دانشکده پزشکی، دانشگاه علوم پزشکی مازندران، ساری، ایران
Nabiallah
Mohammadi
نبی اله
محمدی
mohammadi.nabi@yahoo.com
1003194753284600124903
1003194753284600124903
No
PhD Student in Immunology, Immunogenetics Research center, Department of Immunology, Mazandaran University of Medical Sciences, Sari, Iran
دانشجوی دکترا ایمونولوژی، مرکز تحقیقات ژنتیک ایمنی، گروه ایمونولوژی، دانشگاه علوم پزشکی مازندران، ساری، ایران
Saeid
Abediankenari
سعید
عابدیان کناری
1003194753284600124904
1003194753284600124904
Yes
Professor, Immunogenetics Research center, Department of Immunology, Mazandaran University of Medical Sciences, Sari, Iran
استاد، مرکز تحقیقات ژنتیک ایمنی، گروه ایمونولوژی، دانشگاه علوم پزشکی مازندران، ساری، ایران