Journal of Mazandaran University of Medical Sciences
مجله دانشگاه علوم پزشکی مازندران
J Mazandaran Univ Med Sci
Medical Sciences
http://jmums.mazums.ac.ir
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2022
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آنالیز بررسی بیان ژن و مسیرهای سیگنالینگ و شبکه های برهمکنش برخی ژن های موثر در بیماران آسم در مطالعات
حاصل از مایکروارای با کمک نرم افزار R
Analysis of Gene Expression, Signaling Pathways, and Interaction Networks of Some Effective Genes in Patients with Asthma in Microarray Studies Using R Software
ریه
Lung
مروری
Review
<span style="font-size:12pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><span lang="FA" style="font-family:"B Koodak""><span style="color:black"><span style="letter-spacing:-.1pt">سابقه</span></span></span><span lang="AR-SA" style="font-family:"B Koodak""><span style="color:black"><span style="letter-spacing:-.1pt"> و هدف:</span></span></span> <span lang="AR-SA" style="background:white"><span style="font-family:"B Zar""><span style="color:#0d0d0d">آسم</span></span></span> <span lang="AR-SA" style="background:white"><span style="font-family:"B Zar""><span style="color:#0d0d0d">یک بیماری</span></span></span> <span lang="AR-SA" style="background:white"><span style="font-family:"B Zar""><span style="color:#0d0d0d">رایج</span></span></span> <span lang="AR-SA" style="background:white"><span style="font-family:"B Zar""><span style="color:#0d0d0d">التهابی مزمن مجاری هوایی است و در اثر ترکیبی از تعاملات پیچیده محیطی <span style="letter-spacing:-.1pt">و ژنتیکی ایجاد میشود که درک ناقصی از آنها داریم</span></span></span></span><span lang="FA" style="background:white"><span style="font-family:"B Zar""><span style="color:#0d0d0d"><span style="letter-spacing:-.1pt">. </span></span></span></span><span lang="AR-SA" style="background:white"><span style="font-family:"B Zar""><span style="color:#0d0d0d"><span style="letter-spacing:-.1pt">این عوامل شدت بیماری آسم و نیز نحوه پاسخ آن به درمان را تحت</span></span></span></span><span lang="AR-SA" style="background:white"><span style="font-family:"B Zar""><span style="color:#0d0d0d"> تأثیر قرار میدهند.</span></span></span><span lang="FA" style="font-family:"B Zar""><span style="color:#0d0d0d"> بیان متفاوت ژن در بیماران مبتلا به آسم در مقابل گروه شاهد در چندین مطالعه گزارش شده است</span></span><span dir="LTR" style="font-size:10.0pt"><span style="color:#0d0d0d">.</span></span><span lang="FA" style="font-family:"B Zar""><span style="color:#0e101a"></span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><span lang="AR-SA" style="font-family:"B Koodak""><span style="color:black"><span style="letter-spacing:-.1pt">مواد و روش</span></span></span><span lang="FA" style="font-family:"B Zar""><span style="color:black"><span style="letter-spacing:-.1pt"></span></span></span><span lang="AR-SA" style="font-family:"B Koodak""><span style="color:black"><span style="letter-spacing:-.1pt">ها:</span></span></span> <span lang="FA" style="font-family:"B Zar""><span style="color:#0d0d0d">مطالعه حاضر در صدد شناسایی لیست مشترکی از ژن</span></span><span lang="AR-SA" style="background:white"><span style="font-family:"B Zar""><span style="color:#0d0d0d"></span></span></span><span lang="FA" style="font-family:"B Zar""><span style="color:#0d0d0d">های متفاوت بیان شده </span></span><span dir="LTR" style="font-size:10.0pt"><span style="color:#0d0d0d">(DEG)</span></span><span style="font-family:"B Zar""><span style="color:#0d0d0d"> با استفاده از روش</span></span><span lang="AR-SA" style="background:white"><span style="font-family:"B Zar""><span style="color:#0d0d0d"></span></span></span><span lang="FA" style="font-family:"B Zar""><span style="color:#0d0d0d">های بیوانفورماتیکی بود. </span></span><span lang="FA" style="font-family:"B Zar""><span style="color:#0d0d0d">سه مطالعه از داده</span></span><span lang="AR-SA" style="background:white"><span style="font-family:"B Zar""><span style="color:#0d0d0d"></span></span></span><span lang="FA" style="font-family:"B Zar""><span style="color:#0d0d0d">های مایکروارای (</span></span><span dir="LTR" style="font-size:10.0pt"><span style="color:#0d0d0d">microarray</span></span><span lang="FA" style="font-family:"B Zar""><span style="color:#0d0d0d">)،که معیارهای ورود به سیستم تعریف شده <span style="letter-spacing:-.1pt">دارند، شناسایی شد. این مطالعات در مجموع 268 نمونه را شامل می</span></span></span><span lang="FA" style="font-family:"B Zar""><span style="letter-spacing:-.1pt"><span style="color:#0d0d0d">شدند که در آن</span></span></span><span lang="AR-SA" style="background:white"><span style="font-family:"B Zar""><span style="color:#0d0d0d"><span style="letter-spacing:-.1pt"></span></span></span></span><span lang="FA" style="font-family:"B Zar""><span style="color:#0d0d0d"><span style="letter-spacing:-.1pt">ها، 167 فرد بیمار و 101 فرد دیگر سالم</span></span></span><span lang="FA" style="font-family:"B Zar""><span style="color:#0d0d0d"> بودند. این مطالعات تجزیه و تحلیل شدند و سپس یک متاآنالیز بر روی آن</span></span><span lang="AR-SA" style="background:white"><span style="font-family:"B Zar""><span style="color:#0d0d0d"></span></span></span><span lang="FA" style="font-family:"B Zar""><span style="color:#0d0d0d">ها انجام شد.</span></span><span lang="FA" style="font-family:"B Zar""><span style="color:black"></span></span></span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><span lang="AR-SA" style="font-family:"B Koodak""><span style="color:black"><span style="letter-spacing:-.2pt">یافته</span></span></span><span lang="FA" style="font-family:"B Zar""><span style="color:black"><span style="letter-spacing:-.2pt"></span></span></span><span lang="AR-SA" style="font-family:"B Koodak""><span style="color:black"><span style="letter-spacing:-.2pt">ها:</span></span></span> <span lang="FA" style="font-family:"B Zar""><span style="color:#0d0d0d">با شناسایی ژن</span></span><span lang="AR-SA" style="background:white"><span style="font-family:"B Zar""><span style="color:#0d0d0d"></span></span></span><span lang="FA" style="font-family:"B Zar""><span style="color:#0d0d0d">های مشترک در مطالعات مختلف که دچار کاهش و یا افزایش بیان معنادار شده بودند، از آن</span></span><span lang="AR-SA" style="background:white"><span style="font-family:"B Zar""><span style="color:#0d0d0d"></span></span></span><span lang="FA" style="font-family:"B Zar""><span style="color:#0d0d0d">ها در بررسی مسیرهای بیولوژیکی و مولکولی</span></span><span lang="FA" style="font-family:"B Zar""><span style="color:#0d0d0d"> و سلولی استفاده شد که درکل، مشخص شد این مجموعه از ژن</span></span><span lang="AR-SA" style="background:white"><span style="font-family:"B Zar""><span style="color:#0d0d0d"></span></span></span><span lang="FA" style="font-family:"B Zar""><span style="color:#0d0d0d">ها می</span></span><span lang="AR-SA" style="background:white"><span style="font-family:"B Zar""><span style="color:#0d0d0d"></span></span></span><span lang="FA" style="font-family:"B Zar""><span style="color:#0d0d0d">توانند در آسم و برخی مسیرهای موثر در فرایند عملکردی این بیماری نقش داشته باشند.</span></span><span lang="FA" style="font-family:"B Zar""><span style="color:black"></span></span></span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><span lang="AR-SA" style="font-family:"B Koodak""><span style="color:black"><span style="letter-spacing:-.2pt">استنتاج:</span></span></span> <span lang="FA" style="font-family:"B Zar""><span style="color:#0d0d0d"><span style="letter-spacing:-.1pt">براساس یافته</span></span></span><span lang="AR-SA" style="background:white"><span style="font-family:"B Zar""><span style="color:#0d0d0d"><span style="letter-spacing:-.1pt"></span></span></span></span><span lang="FA" style="font-family:"B Zar""><span style="color:#0d0d0d"><span style="letter-spacing:-.1pt">های مطالعه حاضر، می</span></span></span><span lang="AR-SA" style="background:white"><span style="font-family:"B Zar""><span style="color:#0d0d0d"><span style="letter-spacing:-.1pt"></span></span></span></span><span lang="FA" style="font-family:"B Zar""><span style="color:#0d0d0d"><span style="letter-spacing:-.1pt">توان با بررسی اهداف اثرگذاری و آنالیز عملکردی ژن</span></span></span><span lang="AR-SA" style="background:white"><span style="font-family:"B Zar""><span style="color:#0d0d0d"><span style="letter-spacing:-.1pt"></span></span></span></span><span lang="FA" style="font-family:"B Zar""><span style="color:#0d0d0d"><span style="letter-spacing:-.1pt">های جدید موثر در</span></span></span><span lang="FA" style="font-family:"B Zar""><span style="color:#0d0d0d"> بیماری آسم و محصولات پروتیینی آن</span></span><span lang="AR-SA" style="background:white"><span style="font-family:"B Zar""><span style="color:#0d0d0d"></span></span></span><span lang="FA" style="font-family:"B Zar""><span style="color:#0d0d0d">ها و همچنین بررسی نقش درمسیرهای برهمکنشی بیماری آسم، راهکارهای درمانی مناسبی برای مهار عوارض و آسیب</span></span><span lang="AR-SA" style="background:white"><span style="font-family:"B Zar""><span style="color:#0d0d0d"></span></span></span><span lang="FA" style="font-family:"B Zar""><span style="color:#0d0d0d">های بیماری آسم ارائه کرد.</span></span><span lang="AR-SA" style="font-family:"B Zar""><span style="color:black"><span style="letter-spacing:-.2pt"></span></span></span></span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="line-height:135%"><span style="font-family:"Times New Roman","serif""><b><span style="font-size:10.0pt"><span style="line-height:135%"><span style="font-family:"Arial","sans-serif""><span style="letter-spacing:-.2pt">Background and purpose:</span></span></span></span></b> <span style="font-size:10.0pt"><span style="line-height:135%">Asthma is a chronic inflammatory disorder of the airways caused by a combination of complex environmental and genetic interactions. There is an incomplete understanding of this mechanism which affect both severity of the disease and how it responds to treatment. Different gene expressions are reported in patients with asthma and healthy controls.</span></span></span></span></span><br>
<span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:135%"><span style="text-autospace:none"><span style="font-family:"Times New Roman","serif""><b><span style="font-size:10.0pt"><span style="line-height:135%"><span style="font-family:"Arial","sans-serif"">Materials and methods:</span></span></span></b><b> </b><span style="font-size:10.0pt"><span style="line-height:135%">In this study, a common list of different genes expressed (DEG) was identified using bioinformatics methods. Three studies from microarray data that met the study’s inclusion criteria were selected. These studies included 268 samples, consisting of 167 patients and 101 healthy people. These studies were analyzed, and then a meta-analysis was performed.</span></span></span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="line-height:135%"><span style="font-family:"Times New Roman","serif""><b><span style="font-size:10.0pt"><span style="line-height:135%"><span style="font-family:"Arial","sans-serif"">Results:</span></span></span></b><b> </b><span lang="EN" style="font-size:10.0pt"><span style="line-height:135%">Common genes that led to significant decrease or increase in gene expressions were identified and their biological, molecular, and cellular pathways were examined. In general, it was found that this set of genes can be involved in asthma and some pathways affecting the function of disease. </span></span></span></span></span><br>
<span style="font-size:12pt"><span style="line-height:135%"><span style="font-family:"Times New Roman","serif""><b><span lang="EN" style="font-size:10.0pt"><span style="line-height:135%"><span style="font-family:"Arial","sans-serif"">Conclusion:</span></span></span></b><span lang="EN" style="font-size:10.0pt"><span style="line-height:135%"> According to current findings, examining the objectives of efficacy and functional analysis of new genes effective in asthma and their protein products, and also investigating the role of interactions in asthma could help in taking appropriate treatment strategies to control asthma complications and injuries.</span></span><span style="font-size:10.0pt"><span style="line-height:135%"></span></span></span></span></span><br>
آسم, بیان ژن, نرم افزار R, شبکه برهمکنش ژنی
asthma, gene expression, R software, gene interaction network
109
124
http://jmums.mazums.ac.ir/browse.php?a_code=A-10-14225-1&slc_lang=fa&sid=1
Saeed
Pirmoradi
سعید
پیرمرادی
pirmoradi150@gmail.com
1003194753284600143125
1003194753284600143125
Yes
PhD in Biochemistry, Department of Biochemistry, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
دکتری بیوشیمی، گروه بیوشیمی، دانشکده دامپزشکی، دانشگاه شهید چمران اهواز، اهواز، ایران