fa تاثیر انفوزیون اینترا لیپید بر پیامد بارداری در زنان نابارور با سابقه لانه‌گزینی ناموفق (کارآزمایی بالینی تصادفی یک سوکور) زنان و زایمان و نازایی Gynecology and Infertility پژوهشي-کامل Research(Original) &nbsp;<span style="font-size:12pt"><span style="line-height:82%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span lang="FA" style="line-height:82%"><span b="" koodak="" style="font-family:"><span style="color:black"><span style="letter-spacing:-.1pt">سابقه</span></span></span></span><span lang="AR-SA" style="line-height:82%"><span b="" koodak="" style="font-family:"><span style="color:black"><span style="letter-spacing:-.1pt"> و هدف:</span></span></span></span> <span b="" style="font-family:" zar=""><span style="color:black"><span style="letter-spacing:-.2pt">یکی از مشکلات سیکل</span></span></span><span lang="AR-SA" style="line-height:82%"><span b="" style="font-family:" zar="">‌</span></span><span b="" style="font-family:" zar=""><span style="color:black"><span style="letter-spacing:-.2pt">های لقاح آزمایشگاهی شکست مکرر لانه</span></span></span><span lang="AR-SA" style="line-height:82%"><span b="" style="font-family:" zar="">‌</span></span><span b="" style="font-family:" zar=""><span style="color:black"><span style="letter-spacing:-.2pt">گزینی است. مطالعه حاضر با هدف بررسی نتایج حاملگی با استفاده </span></span></span><span lang="FA" style="line-height:82%"><span b="" style="font-family:" zar=""><span style="color:black"><span style="letter-spacing:-.2pt">از انفوزیون اینترالیپید در زنان نابارور با سابقه دو بار شکست لانه</span></span></span></span><span lang="AR-SA" style="line-height:82%"><span b="" style="font-family:" zar="">‌</span></span><span lang="FA" style="line-height:82%"><span b="" style="font-family:" zar=""><span style="color:black"><span style="letter-spacing:-.2pt">گزینی انجام شد.</span></span></span></span><span lang="FA" style="line-height:82%"><span b="" style="font-family:" zar=""><span style="color:#0e101a"></span></span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:82%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span lang="AR-SA" style="line-height:82%"><span b="" koodak="" style="font-family:"><span style="color:black">مواد و روش</span></span></span><span lang="FA" style="line-height:82%"><span b="" style="font-family:" zar=""><span style="color:black">‌</span></span></span><span lang="AR-SA" style="line-height:82%"><span b="" koodak="" style="font-family:"><span style="color:black">ها:</span></span></span> <span lang="FA" style="line-height:82%"><span b="" style="font-family:" zar=""><span style="color:black">این مطالعه کارآزمایی بالینی در سال 1399بر روی 80 زن نابارور در مرکز ناباروری بیمارستان امام خمینی ساری انجام شد. </span></span></span><span lang="AR-SA" style="line-height:82%"><span b="" style="font-family:" zar=""><span style="color:black">در تمامی نمونه</span></span></span><span lang="AR-SA" style="line-height:82%"><span b="" style="font-family:" zar="">‌</span></span><span lang="AR-SA" style="line-height:82%"><span b="" style="font-family:" zar=""><span style="color:black">ها سطح سلول</span></span></span><span lang="AR-SA" style="line-height:82%"><span b="" style="font-family:" zar="">‌</span></span><span lang="AR-SA" style="line-height:82%"><span b="" style="font-family:" zar=""><span style="color:black">های کشنده طبیعی خون محیطی</span></span></span> <span lang="AR-SA" style="line-height:82%"><span b="" style="font-family:" zar=""><span style="color:black">دو هفته قبل از مداخله با استفاده از فلوسیتومتری اندازه</span></span></span><span lang="AR-SA" style="line-height:82%"><span b="" style="font-family:" zar="">‌</span></span><span lang="AR-SA" style="line-height:82%"><span b="" style="font-family:" zar=""><span style="color:black">گیری شد. سپس نمونه</span></span></span><span lang="AR-SA" style="line-height:82%"><span b="" style="font-family:" zar="">‌</span></span><span lang="AR-SA" style="line-height:82%"><span b="" style="font-family:" zar=""><span style="color:black">ها توسط نرم</span></span></span><span lang="AR-SA" style="line-height:82%"><span b="" style="font-family:" zar="">‌</span></span><span lang="AR-SA" style="line-height:82%"><span b="" style="font-family:" zar=""><span style="color:black">افزار آماری</span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:82%"><span style="color:black">SAS</span></span></span> <span lang="AR-SA" style="line-height:82%"><span b="" style="font-family:" zar=""><span style="color:black">به</span></span></span><span lang="AR-SA" style="line-height:82%"><span b="" style="font-family:" zar="">‌</span></span><span lang="AR-SA" style="line-height:82%"><span b="" style="font-family:" zar=""><span style="color:black">صورت تصادفی </span></span></span><span lang="FA" style="line-height:82%"><span b="" style="font-family:" zar=""><span style="color:black">به دو </span></span></span><span lang="AR-SA" style="line-height:82%"><span b="" style="font-family:" zar=""><span style="color:black">گروه مداخله و کنترل </span></span></span><span lang="FA" style="line-height:82%"><span b="" style="font-family:" zar=""><span style="color:black">(40 نفر در هرگروه)</span></span></span> <span lang="AR-SA" style="line-height:82%"><span b="" style="font-family:" zar=""><span style="color:black">تقسیم شدند. </span></span></span><span lang="FA" style="line-height:82%"><span b="" style="font-family:" zar=""><span style="color:black">دو روز قبل از انتقال جنین، بیماران در گروه مداخله تحت انفوزیون اینترالیپید (به میزان 2 میلی</span></span></span><span lang="AR-SA" style="line-height:82%"><span b="" style="font-family:" zar="">‌</span></span><span lang="FA" style="line-height:82%"><span b="" style="font-family:" zar=""><span style="color:black">لیتر از محلول 20 درصد در 250 میلی</span></span></span><span lang="AR-SA" style="line-height:82%"><span b="" style="font-family:" zar="">‌</span></span><span lang="FA" style="line-height:82%"><span b="" style="font-family:" zar=""><span style="color:black">لیتر سالین استریل) در طی دو ساعت قرار گرفتند و در گروه کنترل انفوزیون نرمال سالین (250 میلی</span></span></span><span lang="AR-SA" style="line-height:82%"><span b="" style="font-family:" zar="">‌</span></span><span lang="FA" style="line-height:82%"><span b="" style="font-family:" zar=""><span style="color:black">لیتر) صورت گرفت.</span></span></span><span lang="AR-SA" style="line-height:82%"><span b="" style="font-family:" zar=""><span style="color:black"> بارداری بالینی (با مشاهده ضربان قلب جنین در سونوگرافی) در هر دو گروه ثبت و مقایسه شد. داده</span></span></span><span lang="AR-SA" style="line-height:82%"><span b="" style="font-family:" zar="">‌</span></span><span lang="AR-SA" style="line-height:82%"><span b="" style="font-family:" zar=""><span style="color:black">ها با آزمون</span></span></span><span lang="AR-SA" style="line-height:82%"><span b="" style="font-family:" zar="">‌</span></span><span lang="AR-SA" style="line-height:82%"><span b="" style="font-family:" zar=""><span style="color:black">های کای</span></span></span><span lang="AR-SA" style="line-height:82%"><span b="" style="font-family:" zar="">‌</span></span><span lang="AR-SA" style="line-height:82%"><span b="" style="font-family:" zar=""><span style="color:black">دو، </span></span></span><span lang="FA" style="line-height:82%"><span b="" style="font-family:" zar=""><span style="color:black">من ویتنی </span></span></span><span lang="AR-SA" style="line-height:82%"><span b="" style="font-family:" zar=""><span style="color:black">و </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:82%"><span style="color:black">T-test</span></span></span><span lang="AR-SA" style="line-height:82%"><span b="" style="font-family:" zar=""><span style="color:black"> تحلیل شد</span></span></span><span lang="FA" style="line-height:82%"><span b="" nazanin="" style="font-family:">.</span></span><span lang="FA" style="line-height:82%"><span b="" style="font-family:" zar=""><span style="color:black"></span></span></span></span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:82%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span lang="AR-SA" style="line-height:82%"><span b="" koodak="" style="font-family:"><span style="color:black"><span style="letter-spacing:-.3pt">یافته</span></span></span></span><span lang="FA" style="line-height:82%"><span b="" style="font-family:" zar=""><span style="color:black"><span style="letter-spacing:-.3pt">‌</span></span></span></span><span lang="AR-SA" style="line-height:82%"><span b="" koodak="" style="font-family:"><span style="color:black"><span style="letter-spacing:-.3pt">ها:</span></span></span></span> <span lang="AR-SA" style="line-height:82%"><span b="" style="font-family:" zar=""><span style="color:black"><span style="letter-spacing:-.3pt">بارداری بالینی درگروه مداخله پس از انفوزیون اینترالیپید (30 درصد) و در گروه کنترل (10</span></span></span></span> <span lang="AR-SA" style="line-height:82%"><span b="" style="font-family:" zar=""><span style="color:black"><span style="letter-spacing:-.3pt">درصد) بود (0/025=P</span></span></span></span><span lang="AR-SA" style="line-height:82%"><span b="" style="font-family:" zar=""><span style="color:black"><span style="letter-spacing:-.3pt">) و در سطح سلول</span></span></span></span><span lang="AR-SA" style="line-height:82%"><span b="" style="font-family:" zar="">‌</span></span><span lang="AR-SA" style="line-height:82%"><span b="" style="font-family:" zar=""><span style="color:black"><span style="letter-spacing:-.3pt">های کشنده طبیعی و لنفوسیت</span></span></span></span><span lang="AR-SA" style="line-height:82%"><span b="" style="font-family:" zar="">‌</span></span><span lang="AR-SA" style="line-height:82%"><span b="" style="font-family:" zar=""><span style="color:black"><span style="letter-spacing:-.3pt">های خون محیطی بین دو گروه تفاوت معنی</span></span></span></span><span lang="AR-SA" style="line-height:82%"><span b="" style="font-family:" zar="">‌</span></span><span lang="AR-SA" style="line-height:82%"><span b="" style="font-family:" zar=""><span style="color:black"><span style="letter-spacing:-.3pt">داری وجود نداشت (</span></span></span></span><b>0/05>P</b><span lang="FA" style="line-height:82%"><span b="" style="font-family:" zar=""><span style="color:black"><span style="letter-spacing:-.3pt">).</span></span></span></span></span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:82%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span lang="AR-SA" style="line-height:82%"><span b="" koodak="" style="font-family:"><span style="color:black"><span style="letter-spacing:-.2pt">استنتاج:</span></span></span></span> <span lang="AR-SA" style="line-height:82%"><span b="" style="font-family:" zar=""><span style="color:black">اینترالیپید بارداری بالینی را در زنان با سابقه شکست مکرر لانه گزینی بهبود بخشید، اما نیاز است مطالعات بیش</span></span></span><span lang="AR-SA" style="line-height:82%"><span b="" style="font-family:" zar="">‌</span></span><span lang="AR-SA" style="line-height:82%"><span b="" style="font-family:" zar=""><span style="color:black">تری پیرامون مکانیسم اثرات احتمالی ایمونو</span></span></span><span b="" style="font-family:" zar=""><span style="color:black">لوژیک اینترالیپید بر سلول</span></span><span lang="AR-SA" style="line-height:82%"><span b="" style="font-family:" zar="">‌</span></span><span b="" style="font-family:" zar=""><span style="color:black">های کشنده طبیعی صورت گیرد.</span></span><span lang="AR-SA" style="line-height:82%"><span b="" style="font-family:" zar=""><span style="color:black"><span style="letter-spacing:-.2pt"></span></span></span></span></span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="line-height:82%"><span style="tab-stops:102.0pt 135.0pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span lang="AR-SA" style="font-size:6.0pt"><span style="line-height:82%"><span b="" style="font-family:" zar=""><span style="color:black"></span></span></span></span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="line-height:82%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span lang="FA" style="font-size:10.0pt"><span style="line-height:82%"><span b="" style="font-family:" zar="">شماره ثبت کارآزمایی بالینی : 5</span></span></span><span dir="LTR" style="font-size:8.0pt"><span style="line-height:82%">N</span></span><span lang="FA" style="font-size:10.0pt"><span style="line-height:82%"><span b="" style="font-family:" zar="">20140305016858</span></span></span><span dir="LTR" style="font-size:8.0pt"><span style="line-height:82%">IRCT</span></span><span lang="FA" style="font-size:10.0pt"><span style="line-height:82%"><span style="font-family:"2 Davat""></span></span></span></span></span></span></span></span><br> &nbsp; <strong>&nbsp;</strong><span style="font-size:12pt"><span style="line-height:135%"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:10.0pt"><span style="line-height:135%"><span arial="" style="font-family:"><span style="letter-spacing:-.2pt">Background and purpose:</span></span></span></span></b> <span style="font-size:10.0pt"><span style="line-height:135%">Recurrent implantation failure is one of the problems associated with in vitro fertilization cycles. The aim of this study was to evaluate the results of pregnancy using intralipid infusion in infertile women with history of two implantation failures.</span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:135%"><span style="text-autospace:none"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:10.0pt"><span style="line-height:135%"><span arial="" style="font-family:">Materials and methods:</span></span></span></b><b> </b><span style="font-size:10.0pt"><span style="line-height:135%">A clinical trial was conducted in 80 infertile women in the infertility center in Sari Imam Khomeini Hospital, Iran 2020. In all samples, the level of peripheral blood natural killer (NK) cells was measured two weeks before the intervention using flow cytometry. The participants were divided into an intervention group (n=40) and a control group (n=40) by SAS statistical software. Two days before embryo transfer, patients in intervention group underwent intra lipid infusion (2 ml of 20% solution in 250 ml sterile saline) for two hours and the control group received normal saline (250 ml) infusion. Clinical pregnancy (detection of fetal heart rate by ultrasound) rate was compared between the two groups. Data were analyzed using Chi-square, Mann-whitney, and t-test.</span></span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="line-height:135%"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:10.0pt"><span style="line-height:135%"><span arial="" style="font-family:">Results:</span></span></span></b><b> </b><span style="font-size:10.0pt"><span style="line-height:135%"><span style="font-family:" timesnewromanpsmt="">Clinical pregnancy rate was higher in intervention group after intralipid infusion (30%) compared with the control group (10%) (P=0.025). There were no significant differences between the two groups in the level of NK cells and peripheral blood lymphocytes (P<0.05).</span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="line-height:135%"><span style="vertical-align:top"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:10.0pt"><span style="line-height:135%"><span arial="" style="font-family:">Conclusion:</span></span></span></b><span style="font-size:10.0pt"><span style="line-height:135%"> Intralipid infusion improved clinical pregnancy rate in women with history of recurrent implantation failure. However, further studies are required on the possible mechanism of immunological effects of interlaipid in NK cells.<i></i></span></span></span></span></span></span><br> <span style="font-size:12pt"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:7.0pt"><span style="letter-spacing:-.1pt"></span></span></b></span></span><br> <span style="font-size:12pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times=""><span style="font-size:8.0pt"><span style="line-height:150%"><span style="letter-spacing:-.1pt">(Clinical Trials Registry Number: </span></span></span><span style="font-size:8.0pt"><span style="line-height:150%"><span style="letter-spacing:-.1pt">IRCT20140305016858N5</span></span></span><span style="font-size:8.0pt"><span style="line-height:150%"><span style="letter-spacing:-.1pt">)</span></span></span><b><i><span style="font-size:8.0pt"><span style="line-height:150%"><span style="letter-spacing:-.1pt"></span></span></span></i></b></span></span></span><br> &nbsp; اینترالیپید, لقاح آزمایشگاهی, شکست مکرر لانه گزینی, ناباروری intralipid, in vitro fertilization, recurrent implantation failure, Infertility 16 26 http://jmums.mazums.ac.ir/browse.php?a_code=A-10-14959-1&slc_lang=fa&sid=1 Sepideh Peivandi سپیده پیوندی dr_peyvandi@yahoo.com 1003194753284600144097 1003194753284600144097 No Associate Professor, Department of Obstetrics and Gynecology, Mazandaran University of Medical Sciences, Sari, Iran دانشیار، مرکز ناباروری، گروه زنان و زایمان، دانشگاه علوم پزشکی مازندران، ساری، ایران Leila Mortazavi لیلا مرتضوی Leila.mtzv66@gmail.com 1003194753284600144098 1003194753284600144098 Yes Resident in Obstetrics and Gynecology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran دستیار زنان و زایمان، دانشکده پزشکی، دانشگاه علوم پزشکی مازندران، ساری، ایران Noushin Gordani نوشین گردانی nooshin.gordani@yahoo.com 1003194753284600144099 1003194753284600144099 No MSc in Counseling in Midwifery, Mazandaran University of Medical Sciences, Sari, Iran کارشناس ارشد مشاوره در مامایی، دانشگاه علوم پزشکی مازندران، ساری، ایران Marzieh Zamaniyan مرضیه زمانیان marziehzamaniyan@gmail.com 1003194753284600144100 1003194753284600144100 No Associate Professor, Department of Obstetrics and Gynecology, Mazandaran University of Medical Sciences, Sari, Iran Diabetes Research Center, Mazandaran University of Medical Sciences, Sari, Iran دانشیار، مرکز ناباروری، گروه زنان و زایمان، دانشگاه علوم پزشکی مازندران، ساری، ایران مرکز تحقیقات دیابت، دانشگاه علوم پزشکی مازندران، ساری، ایران Hossein Asgarian-Omran حسین عسگریان عمران asgarianhossein@yahoo.com 1003194753284600144101 1003194753284600144101 No Associate Professor, Department of Immunology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran Gastrointestinal Cancer Research Center, Non-Communicable Disease Institute, Mazandaran University of Medical Sciences, Sari, Iran دانشیار، گروه ایمونولوژی، دانشگاه علوم پزشکی مازندران، ساری، ایران مرکز تحقیقات سرطان گوارش، انستیتو بیماری‌های غیرواگیر، دانشگاه علوم پزشکی مازندران، ساری، ایران Abolghasem Ajami ابوالقاسم عجمی Ajami36@gmail.com 1003194753284600144102 1003194753284600144102 No Professor, Department of Immunology, Mazandaran University of Medical Sciences, Sari, Iran استاد، گروه ایمونولوژی، دانشگاه علوم پزشکی مازندران، ساری، ایران Mohammad Khademloo محمد خادملو moh.khademloo@gmail.com 1003194753284600144103 1003194753284600144103 No Associate Professor, Department of Community Medicine, Mazandaran University of Medical Sciences, Sari, Iran دانشیار، گروه پزشکی اجتماعی، دانشگاه علوم پزشکی مازندران، ساری، ایران