Journal of Mazandaran University of Medical Sciences
مجله دانشگاه علوم پزشکی مازندران
J Mazandaran Univ Med Sci
Medical Sciences
http://jmums.mazums.ac.ir
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2022
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مقایسه آزمایشگاهی حیات سلولهای فیبروبلاست لثهی انسانی بر داربستهای حاوی کلاژن
In vitro Comparison of Viability of Human Gingival Fibroblast Cells on Collagen Barriers
دندانپزشکی
Dentistry
گزارش کوتاه
Brief Report
<strong> </strong><span style="font-size:12pt"><span style="line-height:89%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><span lang="FA" style="line-height:89%"><span style="font-family:"B Koodak""><span style="color:black"><span style="letter-spacing:-.1pt">سابقه</span></span></span></span><span lang="AR-SA" style="line-height:89%"><span style="font-family:"B Koodak""><span style="color:black"><span style="letter-spacing:-.1pt"> و هدف:</span></span></span></span> <span lang="AR-SA" style="line-height:89%"><span style="font-family:"B Zar"">کشت فیبروبلاست</span></span><span lang="AR-BH" style="line-height:89%"><span style="font-family:"B Zar"">‎</span></span><span lang="AR-SA" style="line-height:89%"><span style="font-family:"B Zar"">ها</span></span><span lang="AR-SA" style="line-height:89%"><span style="font-family:"B Zar"">ی</span></span><span lang="AR-SA" style="line-height:89%"><span style="font-family:"B Zar""> لثه</span></span> <span lang="AR-SA" style="line-height:89%"><span style="font-family:"B Zar"">انسانی بررو</span></span><span lang="AR-SA" style="line-height:89%"><span style="font-family:"B Zar"">ی </span></span><span lang="AR-SA" style="line-height:89%"><span style="font-family:"B Zar"">غشای کلاژنی به عنوان </span></span><span lang="AR-SA" style="line-height:89%"><span style="font-family:"B Zar"">یک </span></span><span lang="AR-SA" style="line-height:89%"><span style="font-family:"B Zar"">روش درمانی جایگزین در رژنراسیون هدا</span></span><span lang="AR-SA" style="line-height:89%"><span style="font-family:"B Zar"">یت </span></span><span lang="AR-SA" style="line-height:89%"><span style="font-family:"B Zar"">شونده به کاررفته است. </span></span><span lang="AR-SA" style="line-height:89%"><span style="font-family:"B Zar"">مطالعه آزمایشگاهی حاضر، حیات فیبروبلاست</span></span><span lang="AR-BH" style="line-height:89%"><span style="font-family:"B Zar"">‎</span></span><span lang="AR-SA" style="line-height:89%"><span style="font-family:"B Zar"">های لثه</span></span><span lang="AR-BH" style="line-height:89%"><span style="font-family:"B Zar"">‎</span></span><span lang="AR-SA" style="line-height:89%"><span style="font-family:"B Zar"">ای را روی دو نوع غشای غنی از کلاژن ارزیابی و مقایسه کرده است</span></span><span lang="AR-SA" style="line-height:89%"><span style="font-family:"B Zar"">.</span></span><span lang="FA" style="line-height:89%"><span style="font-family:"B Zar""><span style="color:#0e101a"></span></span></span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:89%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><span lang="AR-SA" style="line-height:89%"><span style="font-family:"B Koodak""><span style="color:black"><span style="letter-spacing:-.1pt">مواد و روش</span></span></span></span><span lang="FA" style="line-height:89%"><span style="font-family:"B Zar""><span style="color:black"><span style="letter-spacing:-.1pt"></span></span></span></span><span lang="AR-SA" style="line-height:89%"><span style="font-family:"B Koodak""><span style="color:black"><span style="letter-spacing:-.1pt">ها:</span></span></span></span> <span lang="AR-SA" style="line-height:89%"><span style="font-family:"B Zar"">سلول</span></span><span lang="AR-BH" style="line-height:89%"><span style="font-family:"B Zar"">‎</span></span><span lang="AR-SA" style="line-height:89%"><span style="font-family:"B Zar"">های فیبروبلاست لثهای انسان </span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:89%">(HGF1-RT1)</span></span><span lang="AR-SA" style="line-height:89%"><span style="font-family:"B Zar""> روی دو نوع غشای غنی از کلاژن قابل جذب </span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:89%">Regen</span></span><span lang="AR-SA" style="line-height:89%"><span style="font-family:"B Zar""> و </span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:89%">CenoMembrane</span></span><span style="line-height:89%"><span style="font-family:"B Zar""> کشت داده شدند. 24 و 72 ساعت بعد تست </span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:89%">MTT</span></span> <span lang="AR-SA" style="line-height:89%"><span style="font-family:"B Zar"">انجام شد.</span></span><span lang="AR-SA" style="line-height:89%"><span style="font-family:"B Zar""> برای مقایسه میانگین </span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:89%">MTT</span></span><span lang="AR-SA" style="line-height:89%"><span style="font-family:"B Zar""> در گروهها و زمان</span></span><span lang="AR-BH" style="line-height:89%"><span style="font-family:"B Zar"">‎</span></span><span lang="AR-SA" style="line-height:89%"><span style="font-family:"B Zar"">های مختلف از </span></span><strong><span lang="AR-SA" style="line-height:89%"><span style="font-family:"B Zar""><span style="color:black"><span style="font-weight:normal">آزمون</span></span></span></span></strong><strong> </strong><strong><span dir="LTR" style="font-size:10.0pt"><span style="line-height:89%"><span style="color:black"><span style="font-weight:normal">ANOVA</span></span></span></span></strong><strong> </strong><strong><span lang="AR-SA" style="line-height:89%"><span style="font-family:"B Zar""><span style="color:black"><span style="font-weight:normal">و تست تعقیبی </span></span></span></span></strong><strong><span dir="LTR" style="font-size:10.0pt"><span style="line-height:89%"><span style="color:black"><span style="font-weight:normal">Tukey</span></span></span></span></strong><strong> </strong><span lang="AR-SA" style="line-height:89%"><span style="font-family:"B Zar"">استفاده شد.</span></span><span lang="FA" style="line-height:89%"><span style="font-family:"B Zar""><span style="color:black"></span></span></span></span></span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:89%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><span lang="AR-SA" style="line-height:89%"><span style="font-family:"B Koodak""><span style="color:black"><span style="letter-spacing:-.2pt">یافته</span></span></span></span><span lang="FA" style="line-height:89%"><span style="font-family:"B Zar""><span style="color:black"><span style="letter-spacing:-.2pt"></span></span></span></span><span lang="AR-SA" style="line-height:89%"><span style="font-family:"B Koodak""><span style="color:black"><span style="letter-spacing:-.2pt">ها:</span></span></span></span> <span lang="AR-SA" style="line-height:89%"><span style="font-family:"B Zar""><span style="letter-spacing:-.2pt">پس از 24 و 72 ساعت بیش</span></span></span><span lang="AR-BH" style="line-height:89%"><span style="font-family:"B Zar""><span style="letter-spacing:-.2pt">‎</span></span></span><span lang="AR-SA" style="line-height:89%"><span style="font-family:"B Zar""><span style="letter-spacing:-.2pt">ترین حیات سلول</span></span></span><span lang="AR-BH" style="line-height:89%"><span style="font-family:"B Zar""><span style="letter-spacing:-.2pt">‎</span></span></span><span lang="AR-SA" style="line-height:89%"><span style="font-family:"B Zar""><span style="letter-spacing:-.2pt">های فیبروبلاست در گروه</span></span></span><span lang="AR-BH" style="line-height:89%"><span style="font-family:"B Zar""><span style="letter-spacing:-.2pt">‎</span></span></span><span lang="AR-SA" style="line-height:89%"><span style="font-family:"B Zar""><span style="letter-spacing:-.2pt">های کنترل و غشا </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:89%"><span style="letter-spacing:-.2pt">Regen</span></span></span><span lang="AR-SA" style="line-height:89%"><span style="font-family:"B Zar""><span style="letter-spacing:-.2pt"> بود و کم</span></span></span><span lang="AR-BH" style="line-height:89%"><span style="font-family:"B Zar""><span style="letter-spacing:-.2pt">‎</span></span></span><span lang="AR-SA" style="line-height:89%"><span style="font-family:"B Zar""><span style="letter-spacing:-.2pt">ترین</span></span></span><span lang="AR-SA" style="line-height:89%"><span style="font-family:"B Zar""> میزان در </span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:89%">CenoMembrane</span></span><span lang="AR-SA" style="line-height:89%"><span style="font-family:"B Zar""> بود. حیات سلول</span></span><span lang="AR-BH" style="line-height:89%"><span style="font-family:"B Zar"">‎</span></span><span lang="AR-SA" style="line-height:89%"><span style="font-family:"B Zar"">ها در گروه کنترل بهطور معنی</span></span><span lang="AR-BH" style="line-height:89%"><span style="font-family:"B Zar"">‎</span></span><span lang="AR-SA" style="line-height:89%"><span style="font-family:"B Zar"">داری بیشتر از غشای </span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:89%">CenoMembrane</span></span><span style="line-height:89%"><span style="font-family:"B Zar""> و در غشای </span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:89%">Regen</span></span><span style="line-height:89%"><span style="font-family:"B Zar""> بهطور معنی</span></span><span lang="AR-BH" style="line-height:89%"><span style="font-family:"B Zar"">‎</span></span><span lang="AR-SA" style="line-height:89%"><span style="font-family:"B Zar"">داری بیش</span></span><span lang="AR-BH" style="line-height:89%"><span style="font-family:"B Zar"">‎</span></span><span lang="AR-SA" style="line-height:89%"><span style="font-family:"B Zar"">تر از </span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:89%">CenoMembrane</span></span><span style="line-height:89%"><span style="font-family:"B Zar""> بود(0/05</span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:89%">P<</span></span><span lang="AR-SA" style="line-height:89%"><span style="font-family:"B Zar"">) تفاوت آماری معنی</span></span><span lang="AR-BH" style="line-height:89%"><span style="font-family:"B Zar"">‎</span></span><span lang="AR-SA" style="line-height:89%"><span style="font-family:"B Zar"">داری بین گروه کنترل و غشای </span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:89%">Regen</span></span><span style="line-height:89%"><span style="font-family:"B Zar""> وجود نداشت (0/05</span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:89%"><</span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:89%">P</span></span><span lang="AR-SA" style="line-height:89%"><span style="font-family:"B Zar"">).</span></span><span lang="FA" style="line-height:89%"><span style="font-family:"B Zar""><span style="color:black"></span></span></span></span></span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:89%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><span lang="AR-SA" style="line-height:89%"><span style="font-family:"B Koodak""><span style="color:black"><span style="letter-spacing:-.2pt">استنتاج:</span></span></span></span> <span lang="AR-SA" style="line-height:89%"><span style="font-family:"B Zar"">حیات فیبروبلاست</span></span><span lang="AR-BH" style="line-height:89%"><span style="font-family:"B Zar"">‎</span></span><span lang="AR-SA" style="line-height:89%"><span style="font-family:"B Zar"">ها روی غشا </span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:89%">Regen</span></span><span lang="AR-SA" style="line-height:89%"><span style="font-family:"B Zar""> پس از 24 و 72 ساعت براساس تست </span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:89%">MTT</span></span><span lang="AR-SA" style="line-height:89%"><span style="font-family:"B Zar"">، در مقایسه با </span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:89%">CenoMembrane</span></span><span style="line-height:89%"><span style="font-family:"B Zar""> بیش</span></span><span lang="AR-BH" style="line-height:89%"><span style="font-family:"B Zar"">‎</span></span><span lang="AR-SA" style="line-height:89%"><span style="font-family:"B Zar"">تر بود.<span style="color:black"><span style="letter-spacing:-.2pt"></span></span></span></span></span></span></span></span></span></span></span><br>
<strong> </strong><span style="font-size:12pt"><span style="line-height:150%"><span style="font-family:"Times New Roman","serif""><b><span style="font-size:10.0pt"><span style="line-height:150%"><span style="font-family:"Arial","sans-serif""><span style="letter-spacing:-.2pt">Background and purpose:</span></span></span></span></b> <span lang="EN" style="font-size:10.0pt"><span style="line-height:150%">Human gingival fibroblasts cultured on collagen membrane is an alternative treatment method in guided regeneration. This <i>in vitro</i> study aimed at evaluating and comparing the human gingival fibroblasts viability on two types of collagen-rich membranes.</span></span></span></span></span><br>
<span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:150%"><span style="text-autospace:none"><span style="font-family:"Times New Roman","serif""><b><span style="font-size:10.0pt"><span style="line-height:150%"><span style="font-family:"Arial","sans-serif"">Materials and methods:</span></span></span></b><b> </b><span lang="EN" style="font-size:10.0pt"><span style="line-height:150%">Human gingival fibroblast cells (HGF1-RT1) were cultured on two</span></span><span style="font-size:10.0pt"><span style="line-height:150%"> types of</span></span><span lang="EN" style="font-size:10.0pt"><span style="line-height:150%"> collagen-rich membranes, including Regen and CenoMembrane. After 24 and 72 hours, the MTT test was performed. ANOVA test and Post Hoc Tukey were applied to compare MTT mean values in different groups and times.</span></span></span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="line-height:150%"><span style="font-family:"Times New Roman","serif""><b><span style="font-size:10.0pt"><span style="line-height:150%"><span style="font-family:"Arial","sans-serif"">Results:</span></span></span></b><b> </b><span lang="EN" style="font-size:10.0pt"><span style="line-height:150%">After 24 and 72 hours, the highest rates of viability of fibroblast cells were observed in the control group and the Regen membrane, and the lowest viability was seen in the CenoMembrane group. Cell viability in control group was significantly higher than the CenoMembrane and in Regan <span style="letter-spacing:-.1pt">membrane was significantly higher than the CenoMembrane (P<0.05). There were no significant differences</span> between the Regen membrane and the control group (P>0.05).</span></span></span></span></span><br>
<span style="font-size:12pt"><span style="line-height:150%"><span style="vertical-align:top"><span style="font-family:"Times New Roman","serif""><b><span style="font-size:10.0pt"><span style="line-height:150%"><span style="font-family:"Arial","sans-serif"">Conclusion:</span></span></span></b> <span lang="EN" style="font-size:10.0pt"><span style="line-height:150%">Based on MTT assay, the viability of fibroblasts after 24 and 72 hours was higher in the Regen membrane compared with the CenoMembrane.</span></span><i><span style="font-size:10.0pt"><span style="line-height:150%"></span></span></i></span></span></span></span><br>
فیبروبلاست, کشت سلول, Regen, CenoMembrane
fibroblast, cell culture, CenoMembrane, Regen
151
156
http://jmums.mazums.ac.ir/browse.php?a_code=A-10-15037-1&slc_lang=fa&sid=1
Asieh
Mozaffari
آسیه
مظفری
asiehmozaffari@yahoo.com
1003194753284600150167
1003194753284600150167
No
Assistant Professor, Department of Periodontics, Dental Caries Prevention Research Center, Qazvin University of Medical Sciences, Qazvin, Iran
استادیار، گروه پریودانتیکس، مرکز تحقیقات پیشگیری از پوسیدگی دندان، دانشگاه علوم پزشکی قزوین، قزوین، ایران
Mona
Akbari
منا
اکبری
Mona_akbari91@yahoo.com
1003194753284600150168
1003194753284600150168
Yes
Periodontics Resident, Student Research Committee, Qazvin University of Medical Sciences, Qazvin, Iran . Assistant Professor, Department of Periodontics, School of Dentistry, Zanjan University of Medical Sciences, Zanjan, Iran
دستیار تخصصی پریودانتیکس، کمیته تحقیقات دانشجویی، دانشگاه علوم پزشکی قزوین، قزوین، ایران . استادیار، گروه پریودانتیکس، دانشکده دندانپزشکی، دانشگاه علوم پزشکی زنجان، زنجان، ایران
Zohreh
Yazdi
زهره
یزدی
Dr.zyazdi@yahoo.com
1003194753284600150169
1003194753284600150169
No
Associate Professor, Social Determinants of Health Research Center, Qazvin University of Medical Sciences, Qazvin, Iran
دانشیار، مرکز تحقیقات عوامل اجتماعی موثر بر سلامت، دانشگاه علوم پزشکی قزوین، قزوین، ایران
Azadehzeinab
Titidezh
آزاده زینب
تی تی دژ
azadeh_titidezh@yahoo.com
1003194753284600150170
1003194753284600150170
No
Assistant Professor, Department of Oral and Maxillofacial Pathology, Dental Caries Prevention Research Center, Qazvin University of Medical Sciences, Qazvin, Iran
استادیار، گروه پاتولوژی دهان و دندان، مرکز تحقیقات پیشگیری از پوسیدگی دندان، دانشگاه علوم پزشکی قزوین، قزوین، ایران