fa بررسی اثرفرمولاسیون لیپوژل حاوی عصاره رامنوس کاتارتیکا (Rhamnus cathartica L.) بر روی زخم در رت های ویستار فارماکوگنوزی(تهیه دارو از منابع طبیعی) Pharmacognosy پژوهشي-کامل Research(Original) <span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:85%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span lang="FA" style="line-height:85%"><span b="" koodak="" style="font-family:"><span style="color:black"><span style="letter-spacing:-.1pt">سابقه</span></span></span></span><span lang="AR-SA" style="line-height:85%"><span b="" koodak="" style="font-family:"><span style="color:black"><span style="letter-spacing:-.1pt"> و هدف:</span></span></span></span> <span lang="FA" style="line-height:85%"><span b="" style="font-family:" zar="">زخم عارضه</span></span><span lang="AR-SA" style="line-height:85%"><span b="" style="font-family:" zar="">‌</span></span><span lang="FA" style="line-height:85%"><span b="" style="font-family:" zar="">ای با شیوع بالا در جامعه می</span></span><span lang="AR-SA" style="line-height:85%"><span b="" style="font-family:" zar="">‌</span></span><span lang="FA" style="line-height:85%"><span b="" style="font-family:" zar="">باشد و مطالعات مختلفی در زمینه تسـریع رونـد بهبـودی زخم به انجام رسیده است. </span></span><span lang="AR-SA" style="line-height:85%"><span b="" style="font-family:" zar="">هدف از این مطالعه بررسی اثر </span></span><span lang="FA" style="line-height:85%"><span b="" style="font-family:" zar="">فرمولاسیون لیپوژل حاوی عصاره</span></span> <span lang="FA" style="line-height:85%"><span b="" style="font-family:" zar="">رامنوس کاتارتیکا<br> (</span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:85%">Rhamnus cathartica L.</span></span><span lang="FA" style="line-height:85%"><span b="" style="font-family:" zar="">)،</span></span> <span lang="AR-SA" style="line-height:85%"><span b="" style="font-family:" zar="">یکی از گیاهان بومی ایران، </span></span><span lang="FA" style="line-height:85%"><span b="" style="font-family:" zar="">بر بهبود زخم د</span></span><span lang="AR-SA" style="line-height:85%"><span b="" style="font-family:" zar="">ر رت‌های نژاد ویستار بود.</span></span><span lang="FA" style="line-height:85%"><span b="" style="font-family:" zar=""><span style="color:#0e101a"></span></span></span></span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:85%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span lang="AR-SA" style="line-height:85%"><span b="" koodak="" style="font-family:"><span style="color:black"><span style="letter-spacing:-.1pt">مواد و روش</span></span></span></span><span lang="FA" style="line-height:85%"><span b="" style="font-family:" zar=""><span style="color:black"><span style="letter-spacing:-.1pt">‌</span></span></span></span><span lang="AR-SA" style="line-height:85%"><span b="" koodak="" style="font-family:"><span style="color:black"><span style="letter-spacing:-.1pt">ها:</span></span></span></span> <span lang="AR-SA" style="line-height:85%"><span b="" style="font-family:" zar="">در این مطالعه تجربی،<b> </b>جهت تهیه فرمولاسیون لیپوژل، فرمولاسیون‌های متفاوتی با نسبت‌های مختلف <span style="letter-spacing:-.1pt">پارافین، پلی‌اتیلن و عصاره تهیه و برای یافتن بهترین فرمولاسیون آزمایش شد. برای بررسی اثربخشی عصاره در بهبود زخم،</span> <span style="letter-spacing:-.1pt">تعداد </span></span></span><span lang="AR-SA" style="line-height:85%"><span b="" style="font-family:" zar=""><span style="letter-spacing:-.1pt">۲۰</span></span></span><span lang="AR-SA" style="line-height:85%"><span b="" style="font-family:" zar=""><span style="letter-spacing:-.1pt"> رت به چهار گروه (</span></span></span><span lang="AR-SA" style="line-height:85%"><span b="" style="font-family:" zar=""><span style="letter-spacing:-.1pt">۵</span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:85%"><span style="letter-spacing:-.1pt">N=</span></span></span><span lang="FA" style="line-height:85%"><span b="" style="font-family:" zar=""><span style="letter-spacing:-.1pt">) </span></span></span><span lang="AR-SA" style="line-height:85%"><span b="" style="font-family:" zar=""><span style="letter-spacing:-.1pt">نرمال سالین، پایه لیپوژل، لیپوژل عصاره و </span></span></span><span lang="FA" style="line-height:85%"><span b="" style="font-family:" zar=""><span style="letter-spacing:-.1pt">اکتیو واتر تقسیم شدند. زخم کامل به مساحت</span></span></span> <span lang="FA" style="line-height:85%"><span b="" style="font-family:" zar=""><span style="letter-spacing:-.1pt">۲</span></span></span><span lang="FA" style="line-height:85%"><span b="" style="font-family:" zar=""><span style="letter-spacing:-.1pt">&times;</span></span></span><span lang="FA" style="line-height:85%"><span b="" style="font-family:" zar=""><span style="letter-spacing:-.1pt">۲</span></span></span><span lang="FA" style="line-height:85%"><span b="" style="font-family:" zar=""><span style="letter-spacing:-.1pt"> سانتی</span></span></span><span lang="AR-SA" style="line-height:85%"><span b="" style="font-family:" zar=""><span style="letter-spacing:-.1pt">‌</span></span></span><span lang="FA" style="line-height:85%"><span b="" style="font-family:" zar=""><span style="letter-spacing:-.1pt">متردر پشت حیوان ایجاد شد. بررسی ابعاد زخم و مطالعات هیستوپاتولوژی در روزهای </span></span></span><span lang="FA" style="line-height:85%"><span b="" style="font-family:" zar=""><span style="letter-spacing:-.1pt">۳</span></span></span><span lang="FA" style="line-height:85%"><span b="" style="font-family:" zar=""><span style="letter-spacing:-.1pt">، </span></span></span><span lang="FA" style="line-height:85%"><span b="" style="font-family:" zar=""><span style="letter-spacing:-.1pt">۵</span></span></span><span lang="FA" style="line-height:85%"><span b="" style="font-family:" zar=""><span style="letter-spacing:-.1pt">، </span></span></span><span lang="FA" style="line-height:85%"><span b="" style="font-family:" zar=""><span style="letter-spacing:-.1pt">۷ </span></span></span><span lang="FA" style="line-height:85%"><span b="" style="font-family:" zar=""><span style="letter-spacing:-.1pt">و </span></span></span><span lang="FA" style="line-height:85%"><span b="" style="font-family:" zar=""><span style="letter-spacing:-.1pt">۱۲</span></span></span><span lang="FA" style="line-height:85%"><span b="" style="font-family:" zar=""><span style="letter-spacing:-.1pt"> انجام گرفت.<span style="color:black"></span></span></span></span></span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:85%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span lang="AR-SA" style="line-height:85%"><span b="" koodak="" style="font-family:"><span style="color:black"><span style="letter-spacing:-.2pt">یافته</span></span></span></span><span lang="FA" style="line-height:85%"><span b="" style="font-family:" zar=""><span style="color:black"><span style="letter-spacing:-.2pt">‌</span></span></span></span><span lang="AR-SA" style="line-height:85%"><span b="" koodak="" style="font-family:"><span style="color:black"><span style="letter-spacing:-.2pt">ها:</span></span></span></span> <span lang="AR-SA" style="line-height:85%"><span b="" style="font-family:" zar="">فرمولاسیون حاوی ۹</span></span><span lang="AR-SA" style="line-height:85%"><span b="" style="font-family:" zar="">۵</span></span><span lang="AR-SA" style="line-height:85%"><span b="" style="font-family:" zar=""> درصد پارافین و </span></span><span lang="AR-SA" style="line-height:85%"><span b="" style="font-family:" zar="">۵</span></span><span lang="AR-SA" style="line-height:85%"><span b="" style="font-family:" zar=""> درصد پلی‌اتیلن به‌دلیل پوشانندگی بهتر پوستی، قوام مناسب و یکنواختی بهتر، به‌عنوان فرمولاسیون نهایی لیپوژل انتخاب شد. </span></span><span lang="AR-SA" style="line-height:85%"><span b="" style="font-family:" zar="">محتوای تام فنولی و فلاونوئیدی گیاه رامنوس کاتارتیکا به ترتیب برابر با</span></span> <span lang="AR-SA" style="line-height:85%"><span b="" style="font-family:" zar="">4/5</span></span><span lang="AR-SA" style="line-height:85%"><span b="" style="font-family:" zar=""> و </span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:85%">mg/g</span></span><span style="line-height:85%"><span b="" style="font-family:" zar=""> 23/415&nbsp;</span></span><span lang="FA" style="line-height:85%"><span b="" style="font-family:" zar="">شد. </span></span><span lang="AR-SA" style="line-height:85%"><span b="" style="font-family:" zar="">نتایج مطالعات درون‌تنی نشان داد که ابعاد زخم در گروه عصاره نسبت به بقیه گروه‌ها تفاوت معنی‌دار آماری دارد(p0/05</span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:85%">&le;</span></span><span lang="FA" style="line-height:85%"><span b="" style="font-family:" zar="">) و</span></span> <span lang="AR-SA" style="line-height:85%"><span b="" style="font-family:" zar="">گیاه رامنوس کاتارتیکا قدرت خوبی در بهبود زخم داشت. هم‌چنین نتایج هیستوپاتولوژی گواه بر این بود که این گیاه در مواردی همچون رسوب کلاژن، نورگ‌زایی، بلوغ فیبروبلاست و تشکیل اپیدرم نسبت به سایر گروه‌ها اثر بهتری داشت(p0/05</span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:85%">&le;</span></span><span lang="FA" style="line-height:85%"><span b="" style="font-family:" zar="">)</span></span><span lang="AR-SA" style="line-height:85%"><span b="" style="font-family:" zar="">.</span></span><span lang="FA" style="line-height:85%"><span b="" style="font-family:" zar=""><span style="color:black"></span></span></span></span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:85%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span lang="AR-SA" style="line-height:85%"><span b="" koodak="" style="font-family:"><span style="color:black"><span style="letter-spacing:-.2pt">استنتاج:</span></span></span></span> <span b="" lang="FA" style="font-family:" zar="">بر اساس یافته</span><span lang="AR-SA" style="line-height:85%"><span b="" style="font-family:" zar="">‌</span></span><span b="" lang="FA" style="font-family:" zar="">های مطالعه حاضر، لیپوژل حاوی عصاره رامنوس کاتارتیکا در بهبود زخم با ضخامت کامل موثر است.</span><span lang="AR-SA" style="line-height:85%"><span b="" style="font-family:" zar=""><span style="color:black"><span style="letter-spacing:-.2pt"></span></span></span></span></span></span></span></span></span></span></span><br> &nbsp; <span style="font-size:12pt"><span style="line-height:135%"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:10.0pt"><span style="line-height:135%"><span arial="" style="font-family:"><span style="letter-spacing:-.2pt">Background and purpose:</span></span></span></span></b> <span lang="EN" style="font-size:10.0pt"><span style="line-height:135%">Wound is a complication that is highly prevalent. The aim of this study was to investigate the effect of a lipogel formulation containing <i>Rhamnus cathartica</i> L.</span></span><span style="font-size:10.0pt"><span style="line-height:135%">, a native Iranian plant,</span></span><span lang="EN" style="font-size:10.0pt"><span style="line-height:135%"> on wound healing in Wistar rats.</span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:135%"><span style="text-autospace:none"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:10.0pt"><span style="line-height:135%"><span arial="" style="font-family:">Materials and methods:</span></span></span></b><b> </b><span lang="EN" style="font-size:10.0pt"><span style="line-height:135%">In this experimental study, </span></span><span lang="EN" style="font-size:10.0pt"><span style="line-height:135%">to find the best lipogel formula, several formulations with different ratios of paraffin, polyethylene and extract were examined. Twenty rats were divided into four groups (n=5), including normal saline, lipogel base, extract lipogel, and active water. Full-thickness wound of 2*2 cm was induced in the ​​ dorsal area. Wound size evaluation and histopathological study were done at days 3, 5, 7, and 12.</span></span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="line-height:135%"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:10.0pt"><span style="line-height:135%"><span arial="" style="font-family:">Results:</span></span></span></b><b> </b><span lang="EN" style="font-size:10.0pt"><span style="line-height:135%">The formulation containing 95% paraffin and 5% polyethylene was selected due to better skin coverage, good consistency, and better uniformity. Total phenolic content and total flavonoid content of <i>R. cathartica</i> were 4.5 mg/g and 23.415mg/g, respectively. In vivo results indicated significant differences in wound size between the treatment group and other groups (P</span></span><span lang="EN" style="font-size:10.0pt"><span style="line-height:135%">&le;</span></span><span lang="EN" style="font-size:10.0pt"><span style="line-height:135%">0.05). Histopathology studies showed that the <i>R. cathartica</i> extract had a better effect on collagen deposition, necrosis, fibroblast maturation, and epidermis formation compared with other groups (P</span></span><span lang="EN" style="font-size:10.0pt"><span style="line-height:135%">&le;</span></span><span lang="EN" style="font-size:10.0pt"><span style="line-height:135%">0.05).</span></span></span></span></span><br> <span style="font-size:12pt"><span style="line-height:135%"><span style="vertical-align:top"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:10.0pt"><span style="line-height:135%"><span arial="" style="font-family:">Conclusion:</span></span></span></b> <span lang="EN" style="font-size:10.0pt"><span style="line-height:135%">This study showed that lipogel containing the <i>R. cathartica</i> extract was effective in full-thickness wound healing.</span></span><i><span style="font-size:10.0pt"><span style="line-height:135%"></span></span></i></span></span></span></span> بهبود زخم, طب سنتی ایرانی, گیاهان دارویی, لیپوژل, رامنوس کاتاریکا, رت‌های ویستار wound healing, traditional Iranian medicine, herbal medicine, lipogel, Rhamnus cathartica, Wistar rats 1 10 http://jmums.mazums.ac.ir/browse.php?a_code=A-10-11413-12&slc_lang=fa&sid=1 Shakiba Ataeian شکیبا عطاییان shakiba1996atn@gmail.com 1003194753284600149316 1003194753284600149316 No Pharmacy Student, Student Research Committee, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran دانشجوی داروسازی، دانشکده داروسازی، دانشگاه علوم پزشکی مازندران، ساری، ایران Mohammad Azadbakht محمد ازادبخت azadbakht110@gmail.com 1003194753284600149317 1003194753284600149317 Yes Professor, Department of Pharmacognosy, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran استاد، گروه فارماکوگنوزی، دانشکده داروسازی، دانشگاه علوم پزشکی مازندران، ساری، ایران Hossein Asgari rad حسین عسگری راد dr_asgarirad@yahoo.com 1003194753284600149318 1003194753284600149318 No Professor, Department of Pharmaceutics, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran استاد گروه فارماسیوتیکس، دانشکده داروسازی، دانشگاه علوم پزشکی مازندران، ساری، ایران Fereshteh Talebpuor فرشته طالب پور ftaleb2001@yahoo.co.uk 1003194753284600149319 1003194753284600149319 No Professor, Department of Anatomy, Cellular and Molecular Biology Research Center, Mazandaran University of Medical Sciences, Sari, Iran استاد، گروه تشریح، دانشکده پزشکی، دانشگاه علوم پزشکی مازندران، ساری، ایران Fatemeh Akbari فاطمه اکبری akbariftm@yahoo.com 1003194753284600149320 1003194753284600149320 No PhD Student in Pharmacognosy, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran دانشجوی دکترای فارماکوگنوزی، دانشکده داروسازی، دانشگاه علوم پزشکی مازندران، ساری، ایران shervin Amirkhanloo شروین امیرخانلو shervin.amirkhanloo@gmail.com 1003194753284600149321 1003194753284600149321 No PhD Student in Pharmaceutics, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran دانشجوی دکترای فارماسیوتیکس، دانشکده داروسازی، دانشگاه علوم پزشکی مازندران، ساری، ایران