Journal of Mazandaran University of Medical Sciences
مجله دانشگاه علوم پزشکی مازندران
J Mazandaran Univ Med Sci
Medical Sciences
http://jmums.mazums.ac.ir
1
admin
1735-9260
1735-9279
fa
jalali
1401
4
1
gregorian
2022
7
1
32
210
online
1
fulltext
fa
طراحی داربست های ترکیبی چند لایه نانوفیبری و فاکتورهای رشد خونی لیوفیلیزه در فرآیند استئوژنز
Designing Nanofiber Multilayer Composite Scaffolds and Lyophilized Blood Growth Factors in the Process of Osteogenesis
ارتوپدی،تروماتولوژی
Orthopedic & Traumatology
پژوهشي-کامل
Research(Original)
<span style="font-size:12pt"><span style="line-height:92%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><span lang="FA" style="line-height:92%"><span style="font-family:"B Koodak""><span style="letter-spacing:-.1pt">سابقه</span></span></span><span lang="AR-SA" style="line-height:92%"><span style="font-family:"B Koodak""><span style="letter-spacing:-.1pt"> و هدف:</span></span></span> <span lang="FA" style="line-height:92%"><span style="font-family:"B Zar"">مهندسی بافت و سلول درمانی به عنوان درمان</span></span><span lang="AR-SA" style="line-height:92%"><span style="font-family:"B Zar""></span></span><span lang="FA" style="line-height:92%"><span style="font-family:"B Zar"">های امیدوارکننده، این امکان را جهت ترمیم ضایعات و نقایص استخوانی می</span></span><span lang="AR-SA" style="line-height:92%"><span style="font-family:"B Zar""></span></span><span lang="FA" style="line-height:92%"><span style="font-family:"B Zar"">تواند فراهم نماید. داربست</span></span><span lang="AR-SA" style="line-height:92%"><span style="font-family:"B Zar""></span></span><span lang="FA" style="line-height:92%"><span style="font-family:"B Zar"">های ترکیبی، پلیمرهای سنتتیک و طبیعی می</span></span><span lang="AR-SA" style="line-height:92%"><span style="font-family:"B Zar""></span></span><span lang="FA" style="line-height:92%"><span style="font-family:"B Zar"">توانند بستر مناسبی برای </span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%">WJ-MSCs</span></span><span lang="FA" style="line-height:92%"><span style="font-family:"B Zar""> به سلول</span></span><span lang="AR-SA" style="line-height:92%"><span style="font-family:"B Zar""></span></span><span lang="FA" style="line-height:92%"><span style="font-family:"B Zar"">های استخوانی را فراهم سازند. در این مطالعه تاثیر فاکتورهای رشد خونی لیوفیلیزه در پیشبرد فرآیند استءوژنز حاءز اهمیت می باشد.</span></span><span lang="FA" style="line-height:92%"><span style="font-family:"B Zar""></span></span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:92%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><span lang="AR-SA" style="line-height:92%"><span style="font-family:"B Koodak""><span style="letter-spacing:-.1pt">مواد و روش</span></span></span><span lang="FA" style="line-height:92%"><span style="font-family:"B Zar""><span style="letter-spacing:-.1pt"></span></span></span><span lang="AR-SA" style="line-height:92%"><span style="font-family:"B Koodak""><span style="letter-spacing:-.1pt">ها:</span></span></span> <span lang="FA" style="line-height:92%"><span style="font-family:"B Zar"">ابتدا داربست</span></span><span lang="AR-SA" style="line-height:92%"><span style="font-family:"B Zar""></span></span><span lang="FA" style="line-height:92%"><span style="font-family:"B Zar"">های نانولیفی </span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%">PCL-Gel</span></span><span lang="AR-SA" style="line-height:92%"><span style="font-family:"B Zar""> با روش الکتروریسی تهیه و </span></span><span lang="FA" style="line-height:92%"><span style="font-family:"B Zar"">همچنین برای تهیه غشا، از افراد <span style="letter-spacing:-.1pt">داوطلب خون جمع</span></span></span><span lang="AR-SA" style="line-height:92%"><span style="font-family:"B Zar""><span style="letter-spacing:-.1pt"></span></span></span><span lang="FA" style="line-height:92%"><span style="font-family:"B Zar""><span style="letter-spacing:-.1pt">آوری و فاکتورهای رشد آن استخراج و بر روی لایه</span></span></span><span lang="AR-SA" style="line-height:92%"><span style="font-family:"B Zar""><span style="letter-spacing:-.1pt"></span></span></span><span lang="FA" style="line-height:92%"><span style="font-family:"B Zar""><span style="letter-spacing:-.1pt">های نانو فیبری لود گردید. سپس در فریزر </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"inherit","serif""><span style="letter-spacing:-.1pt">C</span></span></span></span><span lang="FA" style="line-height:92%"><span style="font-family:"Cambria","serif""><span style="letter-spacing:-.1pt">°</span></span></span><span lang="FA" style="line-height:92%"><span style="font-family:"B Zar""><span style="letter-spacing:-.1pt"> 20</span></span></span><span lang="FA" style="line-height:92%"><span style="letter-spacing:-.1pt">–</span></span> <span lang="FA" style="line-height:92%"><span style="font-family:"B Zar"">به مدت ۳ روز و در نهایت فریز داریر به</span></span><span lang="AR-SA" style="line-height:92%"><span style="font-family:"B Zar""></span></span><span lang="FA" style="line-height:92%"><span style="font-family:"B Zar"">مدت 48 ساعت قرار گرفت و از لحاظ ساختاری با میکروسکوپ الکترونی بررسی <span style="letter-spacing:-.1pt">و با اشعه گاما استریل گردیدند. در ادامه </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="letter-spacing:-.1pt">WJ-MSCs</span></span></span><span lang="FA" style="line-height:92%"><span style="font-family:"B Zar""><span style="letter-spacing:-.1pt"> بر روی داربست</span></span></span><span lang="AR-SA" style="line-height:92%"><span style="font-family:"B Zar""><span style="letter-spacing:-.1pt"></span></span></span><span lang="FA" style="line-height:92%"><span style="font-family:"B Zar""><span style="letter-spacing:-.1pt">ها کشت داده شد. به منظور بررسی ماندگاری سلول</span></span></span><span lang="AR-SA" style="line-height:92%"><span style="font-family:"B Zar""><span style="letter-spacing:-.1pt"></span></span></span><span lang="FA" style="line-height:92%"><span style="font-family:"B Zar""><span style="letter-spacing:-.1pt">ها</span></span></span><span lang="FA" style="line-height:92%"><span style="font-family:"B Zar""> و </span></span><span lang="FA" style="line-height:92%"><span style="font-family:"B Zar""><span style="letter-spacing:-.1pt">بیان ژن</span></span></span><span lang="AR-SA" style="line-height:92%"><span style="font-family:"B Zar""><span style="letter-spacing:-.1pt"></span></span></span><span lang="FA" style="line-height:92%"><span style="font-family:"B Zar""><span style="letter-spacing:-.1pt">های </span></span></span><span dir="LTR" style="font-size:9.5pt"><span style="line-height:92%"><span style="letter-spacing:-.1pt">ALP</span></span></span><span lang="AR-SA" style="line-height:92%"><span style="font-family:"B Zar""><span style="letter-spacing:-.1pt">، </span></span></span><span dir="LTR" style="font-size:9.5pt"><span style="line-height:92%"><span style="letter-spacing:-.1pt">RUNX2</span></span></span><span lang="AR-SA" style="line-height:92%"><span style="font-family:"B Zar""><span style="letter-spacing:-.1pt">، </span></span></span><span dir="LTR" style="font-size:9.5pt"><span style="line-height:92%"><span style="letter-spacing:-.1pt">COLX</span></span></span><span lang="AR-SA" style="line-height:92%"><span style="font-family:"B Zar""><span style="letter-spacing:-.1pt"> و </span></span></span><span dir="LTR" style="font-size:9.5pt"><span style="line-height:92%"><span style="letter-spacing:-.1pt">COLI</span></span></span><span lang="FA" style="line-height:92%"><span style="font-family:"B Zar""><span style="letter-spacing:-.1pt"> در داربست طراحی شده آزمون</span></span></span><span lang="AR-SA" style="line-height:92%"><span style="font-family:"B Zar""><span style="letter-spacing:-.1pt"></span></span></span><span lang="FA" style="line-height:92%"><span style="font-family:"B Zar""><span style="letter-spacing:-.1pt">های </span></span></span><span dir="LTR" style="font-size:9.5pt"><span style="line-height:92%"><span style="letter-spacing:-.1pt">MTT</span></span></span><span lang="AR-SA" style="line-height:92%"><span style="font-family:"B Zar""><span style="letter-spacing:-.1pt"> و</span></span></span> <span dir="LTR" style="font-size:9.5pt"><span style="line-height:92%"><span style="letter-spacing:-.1pt">Real-Time PCR</span></span></span> <span lang="FA" style="line-height:92%"><span style="font-family:"B Zar""><span style="letter-spacing:-.1pt">انجام پذیرفت.</span></span></span><span lang="FA" style="line-height:92%"><span style="font-family:"B Zar""><span style="letter-spacing:-.1pt"></span></span></span></span></span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:92%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><span lang="AR-SA" style="line-height:92%"><span style="font-family:"B Koodak"">یافته</span></span><span lang="FA" style="line-height:92%"><span style="font-family:"B Zar""></span></span><span lang="AR-SA" style="line-height:92%"><span style="font-family:"B Koodak"">ها:</span></span> <span lang="AR-SA" style="line-height:92%"><span style="font-family:"B Zar"">نتایج حاصل از مطالعه نشان داد ماندگاری و رشد و تکثیر </span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%">WJ-MSCs</span></span><span lang="AR-SA" style="line-height:92%"><span style="font-family:"B Zar""> و بیان ژن</span></span><span lang="AR-SA" style="line-height:92%"><span style="font-family:"B Zar""></span></span><span lang="AR-SA" style="line-height:92%"><span style="font-family:"B Zar"">های ویژه استخوانی در غلظت بیش</span></span><span lang="AR-SA" style="line-height:92%"><span style="font-family:"B Zar""><span style="letter-spacing:-.1pt"></span></span></span><span lang="AR-SA" style="line-height:92%"><span style="font-family:"B Zar"">تر ترکیب فاکتورهای رشد مشتق از خون/ داربست پلی کاپرولاکتون / ژلاتین به طور معنی</span></span><span lang="AR-SA" style="line-height:92%"><span style="font-family:"B Zar""></span></span><span lang="AR-SA" style="line-height:92%"><span style="font-family:"B Zar"">داری بیش</span></span><span lang="AR-SA" style="line-height:92%"><span style="font-family:"B Zar""></span></span><span lang="AR-SA" style="line-height:92%"><span style="font-family:"B Zar"">تر است</span></span> <span lang="FA" style="line-height:92%"><span style="font-family:"B Zar"">(0/001</span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"inherit","serif"">P<</span></span></span><span lang="FA" style="line-height:92%"><span style="font-family:"B Zar"">).</span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"B Zar""></span></span></span></span></span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:92%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><span lang="AR-SA" style="line-height:92%"><span style="font-family:"B Koodak""><span style="letter-spacing:-.2pt">استنتاج:</span></span></span> <span lang="AR-SA" style="line-height:92%"><span style="font-family:"B Zar"">افزایش غلظت فاکتورهای رشد خونی در داربستهای طراحی شده</span></span><span lang="FA" style="line-height:92%"><span style="font-family:"B Zar"">، </span></span><span lang="AR-SA" style="line-height:92%"><span style="font-family:"B Zar"">فرایند استئوژنز را تسهیل</span></span><span lang="FA" style="line-height:92%"><span style="font-family:"B Zar""> کرد.</span></span><span lang="AR-SA" style="line-height:92%"><span style="font-family:"B Zar""><span style="letter-spacing:-.2pt"></span></span></span></span></span></span></span></span></span></span><br>
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<span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:92%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span lang="AR-SA" style="line-height:92%"><span b="" style="font-family:" zar=""><span style="letter-spacing:-.2pt"></span></span></span></span></span></span></span></span></span></span>
<pre>
<span style="font-size:12pt"><span style="line-height:150%"><span style="font-family:"Times New Roman","serif""><b><span style="font-size:10.0pt"><span style="line-height:150%"><span style="font-family:"Arial","sans-serif""><span style="letter-spacing:-.2pt">Background and purpose:</span></span></span></span></b> <span lang="EN" style="font-size:10.0pt"><span style="line-height:150%">Tissue engineering and cell therapy, as promising therapies, provide the opportunity to repair bone lesions and defects. Combined scaffolds, synthetic and natural polymers can provide a suitable structure for differentiation of Wharton Jelly mesenchymal stem cells (WJ-MSCs) </span></span><span lang="EN" style="font-size:10.0pt"><span style="line-height:150%">into bone</span></span><span dir="RTL" lang="AR-SA" style="font-size:10.0pt"><span style="line-height:150%">.</span></span><span lang="EN" style="font-size:10.0pt"><span style="line-height:150%"> In current study, the effect of lyophilized blood growth factors in promoting the process of osteogenesis is important.</span></span></span></span></span>
<span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:150%"><span style="text-autospace:none"><span style="font-family:"Times New Roman","serif""><b><span style="font-size:10.0pt"><span style="line-height:150%"><span style="font-family:"Arial","sans-serif"">Materials and methods:</span></span></span></b><b> </b><span lang="EN" style="font-size:10.0pt"><span style="line-height:150%">In this study, PCL-Gel nanofiber scaffolds were prepared by electrospinning method. To prepare membrane, blood was collected from volunteers and its growth factors were extracted and loaded on nanofiber layers. They were then placed in a freezer at -20°C for three days and finally in a freezer for 48 hours. They were structurally examined by electron microscopy and sterilized by gamma rays. WJ-MSCs were then cultured on scaffolding. MTT and Real-Time PCR tests were performed to evaluate cell viability and expression of ALP, RUNX2, COLX, and COLI genes in the designed scaffold.</span></span></span></span></span></span></span></span>
<span style="font-size:12pt"><span style="line-height:150%"><span style="font-family:"Times New Roman","serif""><b><span style="font-size:10.0pt"><span style="line-height:150%"><span style="font-family:"Arial","sans-serif"">Results:</span></span></span></b><b> </b><span lang="EN" style="font-size:10.0pt"><span style="line-height:150%">Findings showed that the viability, growth</span></span><span style="font-size:10.0pt"><span style="line-height:150%">,</span></span><span lang="EN" style="font-size:10.0pt"><span style="line-height:150%"> proliferation, and </span></span><span style="font-size:10.0pt"><span style="background:#f8f9fa"><span style="line-height:150%">the expression of </span></span></span><span lang="EN" style="font-size:10.0pt"><span style="line-height:150%">WJ-MSCs </span></span><span style="font-size:10.0pt"><span style="background:#f8f9fa"><span style="line-height:150%">and osteogenic-specific genes </span></span></span><span lang="EN" style="font-size:10.0pt"><span style="line-height:150%">were significantly higher at high concentration of blood derived growth factors/ polycaprolactone /gelatin-scaffold combination (P<0.001)</span></span><span style="font-size:10.0pt"><span style="line-height:150%">.</span></span></span></span></span>
<span style="font-size:12pt"><span style="line-height:150%"><span style="vertical-align:top"><span style="font-family:"Times New Roman","serif""><b><span style="font-size:10.0pt"><span style="line-height:150%"><span style="font-family:"Arial","sans-serif"">Conclusion:</span></span></span></b> <span style="font-size:10.0pt"><span style="background:#f8f9fa"><span style="line-height:150%">High concentration of blood derived growth factors in designed scaffolds facilitated the osteogenesis process.</span></span></span><i><span style="font-size:10.0pt"><span style="line-height:150%"><span style="color:black"></span></span></span></i></span></span></span></span>
</pre>
پلی کاپرولاکتون / ژلاتین, فاکتورهای رشد خونی, استخوان, سلولهای بنیادی مشتق از وارتون ژلی
polycaprolactone/gelatin, blood-derived growth factors, bone, Wharton Jelly mesenchymal stem cells
1
12
http://jmums.mazums.ac.ir/browse.php?a_code=A-10-7161-2&slc_lang=fa&sid=1
Mahdieh
Ghiasi
مهدیه
قیاثی
mahdieh.ghiasi@yahoo.com
1003194753284600145794
1003194753284600145794
Yes
PhD in Tissue Engineering, Iranian Tissue Bank and Research Center, Gene, Cell and Tissue Research Institute, Tehran University of Medical Sciences, Tehran, Iran
دکترای مهندسی بافت، مرکز تحقیقات و بانک فرآورده های پیوندی ایران، دانشگاه علوم پزشکی تهران، تهران، ایران
Mohammad
Mehdizadeh
محمد
مهدیزاده
dr.rmohammad@yahoo.com
1003194753284600145795
1003194753284600145795
No
2Associate Professor, Dental and Oral Research Center, Department of Oral and Maxillofacial Surgery, School of Dentistry, Qom University of Medical Sciences, Qom, Iran
دانشیار، گروه جراحی فک و صورت، دانشکده دندانپزشکی، دانشگاه علوم پزشکی قم، قم، ایران
liela
khatibshad
لیلا
خطیب شاد
lkhatibshad1397@gmail.com
1003194753284600145796
1003194753284600145796
No
MSc in Pure Chemistry, Iranian Tissue Bank and Research Center, Gene, Cell and Tissue Research Institute, Tehran University of Medical Sciences, Tehran, Iran
کارشناسی ارشد شیمی محض، مرکز تحقیقات و بانک فرآورده های پیوندی ایران، دانشگاه علوم پزشکی تهران، تهران، ایران