Journal of Mazandaran University of Medical Sciences
مجله دانشگاه علوم پزشکی مازندران
J Mazandaran Univ Med Sci
Medical Sciences
http://jmums.mazums.ac.ir
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2022
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اثر سینرژیستی اکسیژن با فشار بالا و پرده آمنیون انسانی سلول زدایی شده در ترمیم زخم تمام ضخامت دیابتی در موش صحرایی
Synergistic Effect of Hyperbaric Oxygen and Decellularized
Human Amniotic Membrane on Full-Thickness
Diabetic Wound Healing in Rats
پوست
Skin
پژوهشي-کامل
Research(Original)
<strong> </strong><span style="font-size:12pt"><span style="line-height:82%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span lang="FA" style="line-height:82%"><span b="" koodak="" style="font-family:"><span style="color:black"><span style="letter-spacing:-.1pt">سابقه</span></span></span></span> <span lang="AR-SA" style="line-height:82%"><span b="" koodak="" style="font-family:"><span style="color:black"><span style="letter-spacing:-.1pt">و هدف:</span></span></span></span> <span lang="AR-SA" style="line-height:82%"><span b="" style="font-family:" zar="">یکی از شایع</span></span><span lang="FA" style="line-height:82%"><span b="" style="font-family:" zar=""></span></span><span lang="AR-SA" style="line-height:82%"><span b="" style="font-family:" zar="">ترین عوارض حاصل از دیابت، اختلال و نقص در ترمیم زخم</span></span><span lang="FA" style="line-height:82%"><span b="" style="font-family:" zar=""></span></span><span lang="AR-SA" style="line-height:82%"><span b="" style="font-family:" zar="">های بیماران دیابتی است. مطالعه حاضر با هدف بررسی اثر سینرژیستی اکسیژن با فشار بالا (</span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:82%">HBO</span></span><span lang="AR-SA" style="line-height:82%"><span b="" style="font-family:" zar="">) و پرده آمنیون انسانی سلول</span></span><span lang="FA" style="line-height:82%"><span b="" style="font-family:" zar=""></span></span><span lang="AR-SA" style="line-height:82%"><span b="" style="font-family:" zar="">زدایی شده (</span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:82%">dHAM</span></span><span lang="AR-SA" style="line-height:82%"><span b="" style="font-family:" zar="">) در ترمیم زخم تمام ضخامت دیابتی در موش صحرایی انجام شد.</span></span><span lang="FA" style="line-height:82%"><span b="" style="font-family:" zar=""><span style="color:#0e101a"></span></span></span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:82%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span lang="AR-SA" style="line-height:82%"><span b="" koodak="" style="font-family:"><span style="color:black"><span style="letter-spacing:-.1pt">مواد و روش</span></span></span></span><span lang="FA" style="line-height:82%"><span b="" style="font-family:" zar=""><span style="color:black"><span style="letter-spacing:-.1pt"></span></span></span></span><span lang="AR-SA" style="line-height:82%"><span b="" koodak="" style="font-family:"><span style="color:black"><span style="letter-spacing:-.1pt">ها:</span></span></span></span> <span lang="AR-SA" style="line-height:82%"><span b="" style="font-family:" zar="">در مطالعه تجربی حاضر 60 سر موش صحرایی دیابتی نر به صورت تصادفی به 4 گروه 15 عددی تقسیم شدند که شامل زخم بدون درمان (</span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:82%">Diabetic</span></span><span lang="AR-SA" style="line-height:82%"><span b="" style="font-family:" zar="">)، زخم تیمار شده با </span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:82%">HBO</span></span><span lang="AR-SA" style="line-height:82%"><span b="" style="font-family:" zar="">، زخم تیمار شده با </span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:82%">dHAM</span></span><span lang="AR-SA" style="line-height:82%"><span b="" style="font-family:" zar=""> و زخم تیمار شده با </span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:82%">HBO+ dHAM</span></span><span lang="AR-SA" style="line-height:82%"><span b="" style="font-family:" zar=""> بودند. نمونه</span></span><span lang="FA" style="line-height:82%"><span b="" style="font-family:" zar=""></span></span><span lang="AR-SA" style="line-height:82%"><span b="" style="font-family:" zar="">گیری از گروه</span></span><span lang="FA" style="line-height:82%"><span b="" style="font-family:" zar=""></span></span><span lang="AR-SA" style="line-height:82%"><span b="" style="font-family:" zar="">های مورد مطالعه در روزهای 7، 14 و 21 پس از ایجاد زخم انجام شد. ارزیابی</span></span><span lang="FA" style="line-height:82%"><span b="" style="font-family:" zar=""></span></span><span lang="AR-SA" style="line-height:82%"><span b="" style="font-family:" zar="">ها شامل درصد بسته شدن زخم، تراکم کلاژ</span></span><span lang="FA" style="line-height:82%"><span b="" style="font-family:" zar="">ن، </span></span><span lang="AR-SA" style="line-height:82%"><span b="" style="font-family:" zar="">حجم بافت، تعداد سلول</span></span><span lang="FA" style="line-height:82%"><span b="" style="font-family:" zar=""></span></span><span lang="AR-SA" style="line-height:82%"><span b="" style="font-family:" zar="">ها و ارزیابی بیان ژن موثر در ترمیم و رگ زایی (</span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:82%">TGF-β</span></span><span lang="AR-SA" style="line-height:82%"><span b="" style="font-family:" zar=""> و </span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:82%">VEGF</span></span><span lang="AR-SA" style="line-height:82%"><span b="" style="font-family:" zar="">)</span></span><span lang="FA" style="line-height:82%"><span b="" style="font-family:" zar=""> و </span></span><span lang="AR-SA" style="line-height:82%"><span b="" style="font-family:" zar="">ژن</span></span><span lang="FA" style="line-height:82%"><span b="" style="font-family:" zar=""></span></span><span lang="AR-SA" style="line-height:82%"><span b="" style="font-family:" zar="">های التهابی (</span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:82%">TNF-α</span></span><span style="line-height:82%"><span b="" style="font-family:" zar=""> و </span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:82%">IL-1β</span></span><span lang="AR-SA" style="line-height:82%"><span b="" style="font-family:" zar="">) و مشخصه بیومکانیکی بافت زخم بود.</span></span><span lang="FA" style="line-height:82%"><span b="" style="font-family:" zar=""><span style="color:black"></span></span></span></span></span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:82%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span lang="AR-SA" style="line-height:82%"><span b="" koodak="" style="font-family:"><span style="color:black"><span style="letter-spacing:-.2pt">یافته</span></span></span></span><span lang="FA" style="line-height:82%"><span b="" style="font-family:" zar=""><span style="color:black"><span style="letter-spacing:-.2pt"></span></span></span></span><span lang="AR-SA" style="line-height:82%"><span b="" koodak="" style="font-family:"><span style="color:black"><span style="letter-spacing:-.2pt">ها:</span></span></span></span> <span lang="AR-SA" style="line-height:82%"><span b="" style="font-family:" zar="">استفاده ترکیبی از </span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:82%">HBO</span></span><span lang="AR-SA" style="line-height:82%"><span b="" style="font-family:" zar=""> و </span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:82%">dHAM</span></span><span style="line-height:82%"><span b="" style="font-family:" zar=""> سبب افزایش معنی</span></span><span lang="FA" style="line-height:82%"><span b="" style="font-family:" zar=""></span></span><span lang="AR-SA" style="line-height:82%"><span b="" style="font-family:" zar="">دار در سرعت بسته شدن زخم، حجم اپیدرم و درم <span style="letter-spacing:-.1pt">جدید، تراکم سلول</span></span></span><span lang="FA" style="line-height:82%"><span b="" style="font-family:" zar=""><span style="letter-spacing:-.1pt"></span></span></span><span lang="AR-SA" style="line-height:82%"><span b="" style="font-family:" zar=""><span style="letter-spacing:-.1pt">های فیبروبلاست و رگ خونی، تراکم کلاژن، ژن</span></span></span><span lang="FA" style="line-height:82%"><span b="" style="font-family:" zar=""><span style="letter-spacing:-.1pt"></span></span></span><span lang="AR-SA" style="line-height:82%"><span b="" style="font-family:" zar=""><span style="letter-spacing:-.1pt">های ترمیمی و رگ</span></span></span><span lang="FA" style="line-height:82%"><span b="" style="font-family:" zar=""><span style="letter-spacing:-.1pt"></span></span></span><span lang="AR-SA" style="line-height:82%"><span b="" style="font-family:" zar=""><span style="letter-spacing:-.1pt">زایی و نیز خصوصیات بیومکانیکی</span></span></span><span lang="AR-SA" style="line-height:82%"><span b="" style="font-family:" zar=""> نسبت به گروه </span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:82%">Diabetic</span></span><span lang="AR-SA" style="line-height:82%"><span b="" style="font-family:" zar=""> بود</span></span><span lang="FA" style="line-height:82%"><span b="" style="font-family:" zar=""> (</span></span><span lang="AR-SA" style="line-height:82%"><span b="" style="font-family:" zar="">0/05>P</span></span><span dir="LTR" lang="EN" style="font-size:10.0pt"><span style="line-height:82%"></span></span><span lang="AR-SA" style="line-height:82%"><span style="font-family:" b="" zar=""></span></span><span lang="FA" style="line-height:82%"><span b="" style="font-family:" zar="">)</span></span><span lang="AR-SA" style="line-height:82%"><span b="" style="font-family:" zar="">. هم چنین بیان ژن</span></span><span lang="FA" style="line-height:82%"><span b="" style="font-family:" zar=""></span></span><span lang="AR-SA" style="line-height:82%"><span b="" style="font-family:" zar="">های التهابی و نیز تراکم نوتروفیل</span></span><span lang="FA" style="line-height:82%"><span b="" style="font-family:" zar=""></span></span><span lang="AR-SA" style="line-height:82%"><span b="" style="font-family:" zar="">ها در تمامی گروه</span></span><span lang="FA" style="line-height:82%"><span b="" style="font-family:" zar=""></span></span><span lang="AR-SA" style="line-height:82%"><span b="" style="font-family:" zar="">های درمانی، به خصوص گروه ترکیبی نسبت به گروه </span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:82%">Diabetic</span></span><span lang="AR-SA" style="line-height:82%"><span b="" style="font-family:" zar=""> به</span></span><span lang="FA" style="line-height:82%"><span b="" style="font-family:" zar=""></span></span><span lang="AR-SA" style="line-height:82%"><span b="" style="font-family:" zar="">صورت معنی</span></span><span lang="FA" style="line-height:82%"><span b="" style="font-family:" zar=""></span></span><span lang="AR-SA" style="line-height:82%"><span b="" style="font-family:" zar="">دار کاهش یافته بود (0/05>P</span></span><span lang="AR-SA" style="line-height:82%"><span b="" style="font-family:" zar="">).</span></span><span lang="FA" style="line-height:82%"><span b="" style="font-family:" zar=""><span style="color:black"></span></span></span></span></span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:82%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span lang="AR-SA" style="line-height:82%"><span b="" koodak="" style="font-family:"><span style="color:black"><span style="letter-spacing:-.2pt">استنتاج:</span></span></span></span> <span lang="AR-SA" style="line-height:82%"><span b="" style="font-family:" zar="">ترکیب </span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:82%">HBO</span></span><span lang="AR-SA" style="line-height:82%"><span b="" style="font-family:" zar=""> و </span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:82%">dHAM</span></span><span lang="AR-SA" style="line-height:82%"><span b="" style="font-family:" zar=""> در مقایسه با استفاده جداگانه از هر کدام، تأثیر بیشتری در روند بهبودی زخم</span></span><span lang="FA" style="line-height:82%"><span b="" style="font-family:" zar=""></span></span><span lang="AR-SA" style="line-height:82%"><span b="" style="font-family:" zar="">های دیابتی داشته است.<span style="color:black"><span style="letter-spacing:-.2pt"></span></span></span></span></span></span></span></span></span></span></span><br>
<strong> </strong><span style="font-size:12pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:10.0pt"><span style="line-height:115%"><span arial="" style="font-family:"><span style="letter-spacing:-.2pt">Background and purpose:</span></span></span></span></b> <span style="font-size:10.0pt"><span style="line-height:115%">One of the common complications of diabetes is impairment of wound healing. The aim of the present study was to investigate the synergistic effect of hyperbaric oxygen (HBO) therapy and decellularized human amniotic membrane (dHAM) in healing of full-thickness diabetic wounds in rats.</span></span></span></span></span><br>
<span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:115%"><span style="text-autospace:none"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:10.0pt"><span style="line-height:115%"><span arial="" style="font-family:">Materials and methods:</span></span></span></b><b> </b><span style="font-size:10.0pt"><span style="line-height:115%">In this experimental study, 60 diabetic male rats were randomly divided into four groups </span></span><span lang="EN" style="font-size:10.0pt"><span style="line-height:115%">(n=15) as follows: </span></span><span style="font-size:10.0pt"><span style="line-height:115%">untreated diabetic wound, wound treated with HBO, wound treated with dHAM, and wound treated with HBO+dHAM. Sampling was performed after 7, 14, and 21 days following wounding. Assessments included wound closure rate, histological (collagen density) and stereological examinations, expression of genes involved in repair and angiogenesis (TGF-β and VEGF), inflammatory genes (TNF-α and IL-1β), and wound tissue biomechanical properties.</span></span></span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:10.0pt"><span style="line-height:115%"><span arial="" style="font-family:">Results:</span></span></span></b><b> </b><span lang="EN" style="font-size:10.0pt"><span style="line-height:115%">Combined use of dHAM and HBO significantly increased wound closure rate, new epidermis and dermis volume, fibroblast cell and blood vessel density, collagen density, repair and angiogenesis genes, and wound biomechanical properties compared with those in the diabetic group (P<0.05). Also, the expression level of inflammatory genes and neutrophil density significantly decreased in all treatment groups, especially in HBO+dHAM group compared with the diabetic group (P<0.05).</span></span></span></span></span><br>
<span style="font-size:12pt"><span style="line-height:115%"><span style="vertical-align:top"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:10.0pt"><span style="line-height:115%"><span arial="" style="font-family:">Conclusion:</span></span></span></b> <span lang="EN" style="font-size:10.0pt"><span style="line-height:115%">dHAM and HBO had a greater effect on the healing process of diabetic wounds when taken together than when each was taken separately.</span></span><i><span style="font-size:10.0pt"><span style="line-height:115%"><span style="color:black"></span></span></span></i></span></span></span></span><br>
زخم دیابتی, اکسیژن با فشار بالا, پرده آمنیون انسانی
diabetic wound, hyperbaric oxygen, human amniotic membrane
1
15
http://jmums.mazums.ac.ir/browse.php?a_code=A-10-4238-7&slc_lang=fa&sid=1
Mostafa
Izanlu
مصطفی
ایزانلو
nosratiresearcher2020@gmail.com
1003194753284600144090
1003194753284600144090
No
Pathology Resident, Imam Reza Hospital, Mashhad University of Medical Sciences, Mashhad, Iran
دستیار پاتولوژی، گروه پاتولوژی، بیمارستان امام رضا، دانشگاه علوم پزشکی مشهد، مشهد، ایران
Alireza
Khalatbary
علیرضا
خلعتبری
khalat90@yahoo.com
1003194753284600144091
1003194753284600144091
No
Professor, Department of Anatomical Sciences, Molecular and Cell Biology Research Center, Mazandaran University of Medical Sciences, Sari, Iran
استاد، گروه علوم تشریح، مرکز تحقیقات سلولی و مولکولی، دانشگاه علوم پزشکی مازندران، ساری، ایران
Amin
Aliabadi
امین
علی آبادی
1003194753284600144092
1003194753284600144092
No
PhD Student in Pharmaceutics, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran
دانشجوی دکترای فارماسیوتیکس، دانشکده داروسازی، دانشگاه علوم پزشکی شهید بهشتی، تهران، ایران
Sima
Davoodi
سیما
داودی
iranianresearcher2020@gmail.com
1003194753284600144093
1003194753284600144093
No
Dermatology Resident, Imam Reza Hospital, Mashhad University of Medical Sciences, Mashhad, Iran
دستیار پوست، گروه پوست، بیمارستان امام رضا، دانشگاه علوم پزشکی مشهد، مشهد، ایران
Amir
Raoofi
امیر
رئوفی
amirrezaraoofi@yahoo.com
1003194753284600144094
1003194753284600144094
No
Assistant Professor, Department of Anatomical Sciences, Molecular and Cell Biology Research Center, Sabzevar University of Medical Sciences, Sabzevar, Iran
استادیار، گروه علوم تشریح، مرکز تحقیقات سلولی و مولکولی، دانشگاه علوم پزشکی سبزوار، سبزوار، ایران
Faezeh
Modanloo
فائزه
مدانلو
hadisadeghi9494@gmail.com
1003194753284600144095
1003194753284600144095
No
BSc Student in Nursing, Student Research Committee, Mazandaran University of Medical Sciences, Sari, Iran
دانشجوی کارشناسی پرستاری، کمیته تحقیقات دانشجویی، دانشگاه علوم پزشکی مازندران، ساری، ایران
Davood
Nasiry
داود
نصیری
davood1990nasiry@gmail.com
1003194753284600144096
1003194753284600144096
Yes
Assistant Professor, Amol Faculty of Paramedicine, Mazandaran University of Medical Sciences, Sari, Iran
استادیار، گروه علوم تشریح، دانشکده پیراپزشکی آمل، دانشگاه علوم پزشکی مازندران، ساری، ایران