fa بررسی اثرات آلیسین روی نفوذپذیری سد خونی-مغزی، نمره نورولوژیکی و ادم مغزی به دنبال القای ترومای مغزی به روش مارمارو در موش صحرایی نر: یک مطالعه رفتاری، بیوشیمیایی و هیستولوژیکی فيزيولوژي physiology پژوهشي-کامل Research(Original) <span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:95%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span lang="FA" style="line-height:95%"><span b="" koodak="" style="font-family:"><span style="color:black">&nbsp;</span></span></span></span></span></span></span></span></span><span style="line-height:94%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span b="" koodak="" style="font-family:"><span style="color:black"><span style="letter-spacing:-.1pt">سابقه</span></span></span><span b="" koodak="" style="font-family:"><span style="color:black"><span style="letter-spacing:-.1pt"> و هدف:</span></span></span> <span b="" style="font-family:" zar=""><span style="letter-spacing:-.2pt">آلیسین دارای طیف گسترده</span></span><span lang="AR-BH" style="line-height:94%"><span b="" style="font-family:" zar=""><span style="letter-spacing:-.2pt">&lrm;</span></span></span><span b="" style="font-family:" zar=""><span style="letter-spacing:-.2pt">ای از عملکردهای دارویی است که همه آن</span></span><span lang="AR-BH" style="line-height:94%"><span b="" style="font-family:" zar=""><span style="letter-spacing:-.2pt">&lrm;</span></span></span><span b="" style="font-family:" zar=""><span style="letter-spacing:-.2pt">ها را می</span></span><span lang="AR-BH" style="line-height:94%"><span b="" style="font-family:" zar=""><span style="letter-spacing:-.2pt">&lrm;</span></span></span><span b="" style="font-family:" zar=""><span style="letter-spacing:-.2pt">توان در فعالیت</span></span><span lang="AR-BH" style="line-height:94%"><span b="" style="font-family:" zar=""><span style="letter-spacing:-.2pt">&lrm;</span></span></span><span b="" style="font-family:" zar=""><span style="letter-spacing:-.2pt">های ضد</span></span><span b="" style="font-family:" zar=""> <span style="letter-spacing:-.2pt">التهابی، آنتی</span></span><span lang="AR-BH" style="line-height:94%"><span b="" style="font-family:" zar=""><span style="letter-spacing:-.2pt">&lrm;</span></span></span><span b="" style="font-family:" zar=""><span style="letter-spacing:-.2pt">اکسیدانی، ضدقارچی و ضدتومور نشان داد، لذا در این مطالعه نقش محافظت نورونی آلیسین در فرایند ضربه مغزی</span></span><span b="" style="font-family:" zar=""> منتشر در موش صحرایی و هم‌چنین تاثیر آن بر روی میزان اینترلوکین</span><span lang="AR-BH" style="line-height:94%"><span b="" style="font-family:" zar="">&lrm;</span></span><span b="" style="font-family:" zar="">ها و تغییرات هیستولوژیکی مورد بررسی قرار گرفت.<span style="color:#0e101a"></span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:94%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span lang="AR-SA" style="line-height:94%"><span b="" koodak="" style="font-family:"><span style="color:black"><span style="letter-spacing:-.1pt">مواد و روش</span></span></span></span><span lang="FA" style="line-height:94%"><span b="" style="font-family:" zar=""><span style="color:black"><span style="letter-spacing:-.1pt">‌</span></span></span></span><span lang="AR-SA" style="line-height:94%"><span b="" koodak="" style="font-family:"><span style="color:black"><span style="letter-spacing:-.1pt">ها:</span></span></span></span> <span lang="FA" style="line-height:94%"><span b="" nazanin="" style="font-family:">در این مطالعه مداخله ای تجربی، </span></span><span lang="FA" style="line-height:94%"><span b="" style="font-family:" zar="">56 سر موش صحرایی نر نژاد ویستار پس از القای بیهوشی و کانول</span></span><span lang="AR-BH" style="line-height:94%"><span b="" style="font-family:" zar="">&lrm;</span></span><span lang="FA" style="line-height:94%"><span b="" style="font-family:" zar="">گذاری در نای، تحت ضربه مغزی کنترل شده منتشر به روش مار مارو قرار گرفتند و 30 دقیقه بعد به رت</span></span><span lang="AR-BH" style="line-height:94%"><span b="" style="font-family:" zar="">&lrm;&lrm;</span></span><span lang="FA" style="line-height:94%"><span b="" style="font-family:" zar="">ها داروی آلیسین با دوزهای مختلف به صورت داخل صفاقی تزریق شد. در زمان‌های قبل از ضربه، بلافاصله پس از به هوش آمدن <span style="letter-spacing:-.1pt">پس از القای ضربه، 24، 48 و 72 ساعت بعد از ضربه </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:94%"><span style="letter-spacing:-.1pt">Veterinary Coma Scale</span></span></span><span lang="FA" style="line-height:94%"><span b="" style="font-family:" zar=""><span style="letter-spacing:-.1pt"> و تست‌های حرکتی و تعادلی </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:94%"><span style="letter-spacing:-.1pt">Beam Walk</span></span></span><span lang="FA" style="line-height:94%"><span b="" style="font-family:" zar=""> و </span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:94%">Beam Balance</span></span><span style="line-height:94%"><span b="" style="font-family:" zar=""> از رت‌ها گرفته و ثبت شد.<span style="color:black"></span></span></span></span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:94%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span lang="AR-SA" style="line-height:94%"><span b="" koodak="" style="font-family:"><span style="color:black"><span style="letter-spacing:-.2pt">یافته</span></span></span></span><span lang="FA" style="line-height:94%"><span b="" style="font-family:" zar=""><span style="color:black"><span style="letter-spacing:-.2pt">‌</span></span></span></span><span lang="AR-SA" style="line-height:94%"><span b="" koodak="" style="font-family:"><span style="color:black"><span style="letter-spacing:-.2pt">ها:</span></span></span></span> <span lang="FA" style="line-height:94%"><span b="" style="font-family:" zar="">آلیسین در دوزهای 25 و 50 </span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:94%">mg/kg</span></span><span lang="FA" style="line-height:94%"><span b="" style="font-family:" zar=""> می تواند اختلاف</span></span> <span lang="FA" style="line-height:94%"><span b="" style="font-family:" zar="">موارد ذکرشده &nbsp;را نسبت به گروه کنترل (</span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:94%">Sham</span></span><span lang="FA" style="line-height:94%"><span b="" style="font-family:" zar=""> و </span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:94%">Intact</span></span><span lang="FA" style="line-height:94%"><span b="" style="font-family:" zar="">) کم‌تر کند (0/001</span></span><span lang="FA" style="font-size:10.0pt"><span style="line-height:94%"><span b="" style="font-family:" zar="">></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:94%">P</span></span><span lang="FA" style="line-height:94%"><span b="" style="font-family:" zar="">). لازم به ذکر است آلیسین در دوز 25 </span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:94%">mg/kg</span></span><span lang="FA" style="line-height:94%"><span b="" style="font-family:" zar=""> موثرتر بوده است (0/0001</span></span><span lang="FA" style="font-size:10.0pt"><span style="line-height:94%"><span b="" style="font-family:" zar="">></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:94%">P</span></span><span lang="FA" style="line-height:94%"><span b="" style="font-family:" zar="">).<span style="color:black"></span></span></span></span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:94%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span b="" koodak="" style="font-family:"><span style="color:black"><span style="letter-spacing:-.2pt">استنتاج:</span></span></span> <span b="" style="font-family:" zar="">براساس این مطالعه می</span><span lang="FA" style="line-height:94%"><span b="" style="font-family:" zar="">‌</span></span><span b="" style="font-family:" zar="">توان دریافت که آلیسین دارای اثرات محافظت نورونی در مغز بود و توانسته بر روی پیامدهای ناشی از ضربه مغزی اثر بگذارد و هم‌چنین می</span><span lang="FA" style="line-height:94%"><span b="" style="font-family:" zar="">‌</span></span><span b="" style="font-family:" zar="">تواند موجب کاهش ادم مغزی، تسریع در بهبود وضعیت سد خونی- مغزی و نمرات نورولوژیکی و تعادلی شود.</span><span b="" style="font-family:" zar=""><span style="color:black"><span style="letter-spacing:-.2pt"></span></span></span></span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:94%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span b="" style="font-family:" zar=""><span style="color:black"><span style="letter-spacing:-.2pt"></span></span></span></span></span></span></span></span></span></span><br> &nbsp; <span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:150%"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:10.0pt"><span style="line-height:150%"><span arial="" style="font-family:"><span style="letter-spacing:-.2pt">&nbsp;</span></span></span></span></b></span></span></span></span><span style="line-height:150%"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:10.0pt"><span style="line-height:150%"><span arial="" style="font-family:"><span style="letter-spacing:-.2pt">Background and purpose:</span></span></span></span></b> <span style="font-size:10.0pt"><span style="line-height:150%">Allicin has a wide range of pharmacological functions, all of which can be demonstrated in anti-inflammatory, antioxidant, antifungal and anti-tumor activities. In this research, we investigated the neuroprotective role of allicin in the process of diffuse traumatic brain injury and its effect on interleukin levels and histological changes in rats.</span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:150%"><span style="text-autospace:none"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:10.0pt"><span style="line-height:150%"><span arial="" style="font-family:">Materials and methods:</span></span></span></b><b> </b><span style="font-size:10.0pt"><span style="line-height:150%">In this experimental study, Wistar rats (n=56) underwent diffuse controlled brain injury by Marmarou method, and 30 minutes later, the drug was injected intraperitoneally at different doses. Veterinary Coma Scale was used and Beam Walk and Beam Balance movement and balance tests were taken at pre-traumatic times, immediately after recovery from the trauma, and 24, 48, and 72 hours after the trauma.</span></span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:10.0pt"><span style="line-height:150%"><span arial="" style="font-family:">Results:</span></span></span></b><b> </b><span style="font-size:10.0pt"><span style="line-height:150%">Findings showed that allicin at 25 mg/kg and 50 mg/kg can reduce these differences compared to the control group (Sham and Intact) (P<0.001). Allicin was found to be more effective at 25 mg/kg (P<0.0001).</span></span></span></span></span><br> <span style="font-size:12pt"><span style="line-height:150%"><span style="vertical-align:top"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:10.0pt"><span style="line-height:150%"><span arial="" style="font-family:">Conclusion:</span></span></span></b><span style="font-size:10.0pt"><span style="line-height:150%"> Allicin showed neuroprotective effects in the brain and can affect the consequences of trauma, reduce cerebral edema, accelerate improvement of blood-brain barrier, and improve neurological scores and the function of vestibular system. <i></i></span></span></span></span></span></span><br> &nbsp; آلیسین, نوروپروتکتیو, ترومای مغزی, ادم مغزی, سد خونی- مغزی, اینترلوکین‌ها allicin, neuroprotective, brain trauma, brain edema, blood-brain barrier, interleukins 61 75 http://jmums.mazums.ac.ir/browse.php?a_code=A-10-10463-13&slc_lang=fa&sid=1 Seyed Reza Seyedin سید رضا سیدین rezaseyedein1995@gmail.com 1003194753284600151248 1003194753284600151248 No Medical Student, Mazandaran University of Medical Sciences, Sari, Iran دانشجوی پزشکی، دانشکده پزشکی، دانشگاه علوم پزشکی مازندران، ساری، ایران Moslem Mohammadi مسلم محمدی mohammadimo@yahoo.com 1003194753284600151249 1003194753284600151249 No Associate Professor, Department of Physiology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran دانشیار، گروه فیزیولوژی، دانشکده پزشکی، دانشگاه علوم پزشکی مازندران، ساری، ایران Ali Siahposht-Khachaki علی سیاه پشت خاچکی a.siahposht@mazums.ac.ir 1003194753284600151250 1003194753284600151250 Yes Associate Professor, Immunogenetics Research Center, Department of Physiology, Mazandaran University of Medical Sciences, Sari, Iran دانشیار، مرکز تحقیقات ایمونوژنتیک، گروه فیزیولوژی، دانشگاه علوم پزشکی مازندران، ساری، ایران