Journal of Mazandaran University of Medical Sciences
مجله دانشگاه علوم پزشکی مازندران
J Mazandaran Univ Med Sci
Medical Sciences
http://jmums.mazums.ac.ir
1
admin
1735-9260
1735-9279
fa
jalali
1401
8
1
gregorian
2022
11
1
32
214
online
1
fulltext
fa
اثر تمرینات مقاومتی و استقامتی بر مسیر پیام رسانی Nrf2/Keap1 در بافت بیضه رت های مبتلا به دیابت نوع دو
Effect of Resistance and Endurance Trainings on Nrf2/Keap1 Signaling Pathway in Testicular Tissue of Type 2 Diabetic Rats
آناتومي
anatomy
پژوهشي-کامل
Research(Original)
<span style="font-size:12pt"><span style="line-height:87%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span lang="FA" style="line-height:87%"><span b="" koodak="" style="font-family:"><span style="color:black"><span style="letter-spacing:-.1pt">سابقه</span></span></span></span><span lang="AR-SA" style="line-height:87%"><span b="" koodak="" style="font-family:"><span style="color:black"><span style="letter-spacing:-.1pt"> و هدف:</span></span></span></span> <span lang="FA" style="line-height:87%"><span b="" style="font-family:" zar="">مسیر آنتی</span></span><span lang="AR-SA" style="line-height:87%"><span b="" style="font-family:" zar=""></span></span><span lang="FA" style="line-height:87%"><span b="" style="font-family:" zar="">اکسیدانی </span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:87%">Nrf2/Keap1</span></span><span lang="FA" style="line-height:87%"><span b="" style="font-family:" zar=""> در سلول از آسیب سلولی در مقابل استرس اکسیداتیو جلوگیری می کند و این مسیر در اثر دیابت مختل می</span></span><span lang="AR-SA" style="line-height:87%"><span b="" style="font-family:" zar=""></span></span><span lang="FA" style="line-height:87%"><span b="" style="font-family:" zar="">شود. هدف از این مطالعه، بررسی اثر تمرینات ورزشی استقامتی و مقاومتی بر این مسیر در بافت بیضه رت</span></span><span lang="AR-SA" style="line-height:87%"><span b="" style="font-family:" zar=""></span></span><span lang="FA" style="line-height:87%"><span b="" style="font-family:" zar="">های مبتلا به دیابت است.<span style="color:#0e101a"></span></span></span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:87%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span lang="AR-SA" style="line-height:87%"><span b="" koodak="" style="font-family:"><span style="color:black"><span style="letter-spacing:-.1pt">مواد و روش</span></span></span></span><span lang="FA" style="line-height:87%"><span b="" style="font-family:" zar=""><span style="color:black"><span style="letter-spacing:-.1pt"></span></span></span></span><span lang="AR-SA" style="line-height:87%"><span b="" koodak="" style="font-family:"><span style="color:black"><span style="letter-spacing:-.1pt">ها:</span></span></span></span> <span lang="AR-SA" style="line-height:87%"><span b="" style="font-family:" zar="">در</span></span> <span lang="AR-SA" style="line-height:87%"><span b="" style="font-family:" zar="">این</span></span><span lang="FA" style="line-height:87%"><span b="" style="font-family:" zar=""> مطالعه تجربی </span></span><span lang="AR-SA" style="line-height:87%"><span b="" style="font-family:" zar="">48 سر</span></span> <span lang="AR-SA" style="line-height:87%"><span b="" style="font-family:" zar="">رت نر نژاد ویستار</span></span> <span lang="AR-SA" style="line-height:87%"><span b="" style="font-family:" zar="">به</span></span> <span lang="AR-SA" style="line-height:87%"><span b="" style="font-family:" zar="">صورت</span></span> <span lang="AR-SA" style="line-height:87%"><span b="" style="font-family:" zar="">تصادفی</span></span> <span lang="AR-SA" style="line-height:87%"><span b="" style="font-family:" zar="">به 6 گروه</span></span> <span lang="AR-SA" style="line-height:87%"><span b="" style="font-family:" zar="">تقسیم</span></span> <span lang="AR-SA" style="line-height:87%"><span b="" style="font-family:" zar="">شدند</span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:87%">:</span></span><span lang="FA" style="line-height:87%"><span b="" style="font-family:" zar=""> کنترل، کنترل تمرین مقاومتی، کنترل تمرین استقامتی، دیابتی، دیابتی تمرین مقاومتی و دیابتی تمرین استقامتی بعد از 12 ساعت ناشتا نگهداشتن، دیابت در حیوانات از طریق تزریق داخل صفاقی </span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:87%">mg/kg</span></span><span lang="FA" style="line-height:87%"><span b="" style="font-family:" zar="">120 نیکوتین آمید و </span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:87%">mg/kg</span></span><span lang="FA" style="line-height:87%"><span b="" style="font-family:" zar="">65</span></span> <span lang="FA" style="line-height:87%"><span b="" style="font-family:" zar="">استرپتوزوتوسین القا شد. 72 ساعت بعد از القای دیابت، حیوانات به مدت 10 هفته طبق پروتکل تحت تمرینات استقامتی و مقاومتی قرار گرفتند. </span></span><span lang="AR-SA" style="line-height:87%"><span b="" style="font-family:" zar="">پس از پایان تمرینات</span></span><span lang="FA" style="line-height:87%"><span b="" style="font-family:" zar=""> مورد نظر،</span></span> <span lang="FA" style="line-height:87%"><span b="" style="font-family:" zar="">بیان ژن</span></span><span lang="AR-SA" style="line-height:87%"><span b="" style="font-family:" zar=""></span></span><span lang="FA" style="line-height:87%"><span b="" style="font-family:" zar="">های </span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:87%">Nrf2</span></span><span lang="FA" style="line-height:87%"><span b="" style="font-family:" zar="">، </span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:87%">Keap1</span></span><span lang="FA" style="line-height:87%"><span b="" style="font-family:" zar=""> و </span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:87%">HO1</span></span> <span lang="AR-SA" style="line-height:87%"><span b="" style="font-family:" zar="">در </span></span><span lang="FA" style="line-height:87%"><span b="" style="font-family:" zar="">بافت </span></span><span lang="AR-SA" style="line-height:87%"><span b="" style="font-family:" zar="">بیضه به روش </span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:87%">real-time PCR</span></span> <span lang="AR-SA" style="line-height:87%"><span b="" style="font-family:" zar="">اندازهگیری شد.</span></span><span lang="FA" style="line-height:87%"><span b="" style="font-family:" zar=""><span style="color:black"></span></span></span></span></span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:87%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span lang="AR-SA" style="line-height:87%"><span b="" koodak="" style="font-family:"><span style="color:black"><span style="letter-spacing:-.2pt">یافته</span></span></span></span><span lang="FA" style="line-height:87%"><span b="" style="font-family:" zar=""><span style="color:black"><span style="letter-spacing:-.2pt"></span></span></span></span><span lang="AR-SA" style="line-height:87%"><span b="" koodak="" style="font-family:"><span style="color:black"><span style="letter-spacing:-.2pt">ها:</span></span></span></span> <span lang="AR-SA" style="line-height:87%"><span b="" style="font-family:" zar="">نتایج</span></span> <span lang="AR-SA" style="line-height:87%"><span b="" style="font-family:" zar="">نشان داد که </span></span><span lang="FA" style="line-height:87%"><span b="" style="font-family:" zar="">القای </span></span><span lang="AR-SA" style="line-height:87%"><span b="" style="font-family:" zar="">دیابت به صورت معنیداری منجر به کاهش بیان ژن های </span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:87%">Nrf2</span></span><span lang="FA" style="line-height:87%"><span b="" style="font-family:" zar=""> و </span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:87%">HO1</span></span><span lang="FA" style="line-height:87%"><span b="" style="font-family:" zar=""> شد<br>
(0/05</span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:87%">P<</span></span><span lang="FA" style="line-height:87%"><span b="" style="font-family:" zar="">)</span></span> <span lang="FA" style="line-height:87%"><span b="" style="font-family:" zar="">در حالی که بیان </span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:87%">Keap1</span></span><span lang="FA" style="line-height:87%"><span b="" style="font-family:" zar=""> افزایش نشان داد اما این افزایش معنی دار نبود (0/05</span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:87%">P></span></span><span lang="FA" style="line-height:87%"><span b="" style="font-family:" zar="">). انجام </span></span><span lang="AR-SA" style="line-height:87%"><span b="" style="font-family:" zar="">تمرینات استقامتی بهطور معنیداری سطح بیان </span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:87%">Nrf2</span></span><span lang="FA" style="line-height:87%"><span b="" style="font-family:" zar=""> را افزایش داد (0/05</span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:87%">P<</span></span><span lang="FA" style="line-height:87%"><span b="" style="font-family:" zar="">). تمرینات مقاومتی سبب تغییر بیان ژنهای مرتبط با مسیر </span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:87%">Nrf2/Keap1</span></span><span lang="FA" style="line-height:87%"><span b="" style="font-family:" zar=""> شد اما این تغییرات معنی</span></span><span lang="AR-SA" style="line-height:87%"><span b="" style="font-family:" zar=""></span></span><span lang="FA" style="line-height:87%"><span b="" style="font-family:" zar="">دار نبود (0/05</span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:87%">P></span></span><span lang="FA" style="line-height:87%"><span b="" style="font-family:" zar="">).<span style="color:black"></span></span></span></span></span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:87%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span lang="AR-SA" style="line-height:87%"><span b="" koodak="" style="font-family:"><span style="color:black"><span style="letter-spacing:-.2pt">استنتاج:</span></span></span></span> <span lang="FA" style="line-height:87%"><span b="" style="font-family:" zar="">تمرینات ورزشی استقامتی و مقاومتی می توانند منجر به فعال شدن بیان ژن</span></span><span lang="AR-SA" style="line-height:87%"><span b="" style="font-family:" zar=""></span></span><span lang="FA" style="line-height:87%"><span b="" style="font-family:" zar="">های مسیر پیام رسانی </span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:87%">Nrf2/Keap1</span></span><span lang="FA" style="line-height:87%"><span b="" style="font-family:" zar=""> شوند و فعال کردن این مسیر ممکن است منجر به کاهش عوارض ناشی از دیابت بر بیضه شود.</span></span><span lang="AR-SA" style="line-height:87%"><span b="" style="font-family:" zar=""><span style="color:black"><span style="letter-spacing:-.2pt"></span></span></span></span></span></span></span></span></span></span></span>
<span style="font-size:12pt"><span style="line-height:135%"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:10.0pt"><span style="line-height:135%"><span arial="" style="font-family:"><span style="letter-spacing:-.2pt">Background and purpose:</span></span></span></span></b> <span style="font-size:10.0pt"><span style="line-height:135%">The antioxidant<b> </b>Nrf2/Keap1 pathway prevents cellular damages against oxidative stress and this pathway is disrupted following diabetes. The aim of this study was to investigate the effect of endurance and resistance training on antioxidant Nrf2/Keap1 pathway in testicular tissue of diabetic rats.</span></span></span></span></span><br>
<span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:135%"><span style="text-autospace:none"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:10.0pt"><span style="line-height:135%"><span arial="" style="font-family:">Materials and methods:</span></span></span></b><b> </b><span style="font-size:10.0pt"><span style="line-height:135%">In this experimental research, 48 male Wistar rats were randomly divided into six groups: control, resistance training control, endurance training control, diabetic, diabetic resistance training, and diabetic endurance training. Diabetes was induced by intraperitoneal injection of 120 mg/kg nicotinamide and 65 mg/kg streptozotocin after 12 hours of fasting. Seventy two hours after induction of diabetes, resistance and endurance training protocols were performed for 10 weeks. Afterwards, the expression of Keap1, Nrf2, and HO1 genes in testicular tissues were measured by real-time PCR.</span></span></span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="line-height:135%"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:10.0pt"><span style="line-height:135%"><span arial="" style="font-family:">Results:</span></span></span></b><b> </b><span style="font-size:10.0pt"><span style="line-height:135%">According to findings, induction of diabetes significantly decreased the expression of Nrf2 and HO1 (P<0.05), while the expression of Keap1 increased (P>0.05). Endurance training significantly increased the expression level of Nrf2 (P<0.05) and resistance training changed the expression level of genes related to Nrf2/Keap1 pathway</span></span> <span style="font-size:10.0pt"><span style="line-height:135%">(P>0.05).</span></span></span></span></span><br>
<span style="font-size:12pt"><span style="line-height:135%"><span style="vertical-align:top"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:10.0pt"><span style="line-height:135%"><span arial="" style="font-family:">Conclusion:</span></span></span></b> <span style="font-size:10.0pt"><span style="line-height:135%">Endurance and resistance exercises could activate Nrf2/Keap1 signaling pathway on gene expression following diabetes and by activating this pathway the complications of diabetes on testis might be reduced.</span></span><i><span style="font-size:10.0pt"><span style="line-height:135%"></span></span></i></span></span></span></span><br>
<span style="font-size:12pt"><span style="line-height:150%"><span style="tab-stops:121.45pt"><span new="" roman="" style="font-family:" times=""><b><i><span style="font-size:10.0pt"><span style="line-height:150%"></span></span></i></b></span></span></span></span><br>
<br>
دیابت, تمرینات استقامتی, تمرینات مقاومتی,Keap1, Nrf2, HO1
diabetes, endurance training, resistance training, keap1, Nrf2, HO1
24
34
http://jmums.mazums.ac.ir/browse.php?a_code=A-10-10332-3&slc_lang=fa&sid=1
Zahra
Nadi
زهرا
نادی
zahra.nadi223@gmail.com
1003194753284600150235
1003194753284600150235
No
MSc in Anatomy, School of Medicine, Arak University of Medical Sciences, Arak, Iran
کارشناسی ارشد علوم تشریح، دانشکده پزشکی، دانشگاه علوم پزشکی اراک، اراک، ایران
Yusef
Abbasi
یوسف
عباسی
yusef6542@gmail.com
1003194753284600150236
1003194753284600150236
No
Assistant Professor, Department of Anatomy, School of Medicine, Arak University of Medical Sciences, Arak, Iran
استادیار، گروه علوم تشریح، دانشکده پزشکی، دانشگاه علوم پزشکی اراک، اراک، ایران
Farideh
Jalali Mashayekhi
فریده
جلالی مشایخی
mashayekhif@yahoo.com
1003194753284600150237
1003194753284600150237
No
Associate Professor, Department of Biochemistry and Genetics, Arak University of Medical Sciences, Arak, Iran
دانشیار، گروه بیوشیمی و ژنتیک، دانشکده پزشکی، دانشگاه علوم پزشکی اراک، اراک، ایران
Mohammad
Bayat
محمد
بیات
dr.mbayat@arakmu.ac.ir
1003194753284600150238
1003194753284600150238
No
Assistant Professor, Department of Anatomy, School of Medicine, Arak University of Medical Sciences, Arak, Iran
استادیار، گروه علوم تشریح، دانشکده پزشکی، دانشگاه علوم پزشکی اراک، اراک، ایران
Parvindokht
Bayat
پرویندخت
بیات
parvin.bayat@ymail.com
1003194753284600150239
1003194753284600150239
No
Professor, Department of Anatomy, School of Medicine, Arak University of Medical Sciences, Arak, Iran
استاد، گروه علوم تشریح، دانشکده پزشکی، دانشگاه علوم پزشکی اراک، اراک، ایران
Maryam
Baazm
مریم
باعزم
dr.baazm@arakmu.ac.ir
1003194753284600150240
1003194753284600150240
Yes
Associate Professor, Department of Anatomy, School of Medicine, Arak University of Medical Sciences, Arak, Iran
دانشیار، گروه علوم تشریح، دانشکده پزشکی، دانشگاه علوم پزشکی اراک، اراک، ایران