Journal of Mazandaran University of Medical Sciences
مجله دانشگاه علوم پزشکی مازندران
J Mazandaran Univ Med Sci
Medical Sciences
http://jmums.mazums.ac.ir
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2022
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اثر محافظت عصبی اسانس سیاهدانه در برابر دمیلینه تجربی از طریق تنظیم سطح اسیدهای چرب درون زا در بافت نخاع موش های صحرائی
Neuroprotective Effect of Nigella sativa Essential Oil against Experimental Demyelination through Regulation of Endogenous Fatty Acid Levels in Rat Spinal Cord Tissue
فيزيولوژي
physiology
پژوهشي-کامل
Research(Original)
<br>
<span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span lang="FA" style="font-size:8.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black"></span></span></span></span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="line-height:95%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><span lang="FA" style="line-height:95%"><span style="font-family:"B Koodak""><span style="color:black"><span style="letter-spacing:-.1pt">سابقه</span></span></span></span><span lang="AR-SA" style="line-height:95%"><span style="font-family:"B Koodak""><span style="color:black"><span style="letter-spacing:-.1pt"> و هدف:</span></span></span></span> <span lang="FA" style="line-height:95%"><span style="font-family:"B Zar""><span style="color:black">تخریب میلین یکی از علل اصلی بیماری</span></span></span><span lang="AR-SA" style="line-height:95%"><span style="font-family:"B Zar""></span></span><span lang="FA" style="line-height:95%"><span style="font-family:"B Zar""><span style="color:black">های عصبی متنوع است. شواهد زیادی وجود دارد که تجویز اسانس</span></span></span><span lang="AR-SA" style="line-height:95%"><span style="font-family:"B Zar""></span></span><span lang="FA" style="line-height:95%"><span style="font-family:"B Zar""><span style="color:black">های گیاهی ممکن است خطر دمیلیناسیون را کاهش دهد. در این مطالعه، با استفاده از یک رویکرد لیپیدمی، تجویز اسانس سیاه دانه، </span></span></span><i><span dir="LTR" style="font-size:10.0pt"><span style="line-height:95%"><span style="color:black">Nigella sativa</span></span></span></i> <span dir="LTR" style="font-size:10.0pt"><span style="line-height:95%"><span style="color:black">)</span></span></span> <span dir="LTR" style="font-size:10.0pt"><span style="line-height:95%"><span style="color:black">(NS</span></span></span><span lang="FA" style="line-height:95%"><span style="font-family:"B Zar""><span style="color:black"> بر بهبود تغییرات پروفایل اسیدهای چرب نخاعی در مدل دمیلیناسیون ناشی از تجویز داخل نخاعی اتیدیوم بروماید (</span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:95%"><span style="color:black">EB</span></span></span><span lang="FA" style="line-height:95%"><span style="font-family:"B Zar""><span style="color:black">) در موشهای صحرائی بررسی شد.</span></span></span><span lang="FA" style="line-height:95%"><span style="font-family:"B Zar""><span style="color:#0e101a"></span></span></span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:95%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><span lang="AR-SA" style="line-height:95%"><span style="font-family:"B Koodak""><span style="color:black"><span style="letter-spacing:-.1pt">مواد و روش</span></span></span></span><span lang="FA" style="line-height:95%"><span style="font-family:"B Zar""><span style="color:black"><span style="letter-spacing:-.1pt"></span></span></span></span><span lang="AR-SA" style="line-height:95%"><span style="font-family:"B Koodak""><span style="color:black"><span style="letter-spacing:-.1pt">ها:</span></span></span></span> <span lang="FA" style="line-height:95%"><span style="font-family:"B Zar""><span style="color:black">در این مطالعه تجربی، تعداد 30 سر موش صحرایی نر در قالب 5 گروه (6</span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:95%"><span style="color:black">n=</span></span></span><span lang="FA" style="line-height:95%"><span style="font-family:"B Zar""><span style="color:black">) مورد مطالعه قرار گرفتند. گروه</span></span></span><span lang="AR-SA" style="line-height:95%"><span style="font-family:"B Zar""></span></span><span lang="FA" style="line-height:95%"><span style="font-family:"B Zar""><span style="color:black">ها به کنترل بدون درمان، دریافت کننده حلال، درمان شده با دوزهای (2، 5 و 10 میلی</span></span></span><span lang="AR-SA" style="line-height:95%"><span style="font-family:"B Zar""></span></span> <span lang="FA" style="line-height:95%"><span style="font-family:"B Zar""><span style="color:black">لیتر بر کیلوگرم داخل صفاقی) از اسانس سیاه دانه تقسیم بندی شدند. اسانس سیاه دانه تهیه و ترکیب اسیدهای چرب آن توسط کروماتوگرافی گازی</span></span></span><span lang="FA" style="line-height:95%"><span style="color:black">–</span></span><span lang="FA" style="line-height:95%"><span style="font-family:"B Zar""><span style="color:black"> اسپکترومتری جرمی (</span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:95%"><span style="color:black">GC-MS</span></span></span><span lang="FA" style="line-height:95%"><span style="font-family:"B Zar""><span style="color:black">) تعیین شد. موش</span></span></span><span lang="AR-SA" style="line-height:95%"><span style="font-family:"B Zar""></span></span><span lang="FA" style="line-height:95%"><span style="font-family:"B Zar""><span style="color:black">های بزرگ نر بالغ نژاد ویستار تحت تجویز داخل نخاعی </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:95%"><span style="color:black">EB</span></span></span><span lang="FA" style="line-height:95%"><span style="font-family:"B Zar""><span style="color:black"> 2/0درصد قرار گرفتند. از نخاع موش</span></span></span><span lang="AR-SA" style="line-height:95%"><span style="font-family:"B Zar""></span></span><span lang="FA" style="line-height:95%"><span style="font-family:"B Zar""><span style="color:black">ها نمونه</span></span></span><span lang="AR-SA" style="line-height:95%"><span style="font-family:"B Zar""></span></span><span lang="FA" style="line-height:95%"><span style="font-family:"B Zar""><span style="color:black">برداری شد و غلظت هفت نوع مختلف از اسیدهای چرب در هموژنای نخاع بین گروه ها مورد سنجش و مقایسه قرار گرفت.</span></span></span><span lang="FA" style="line-height:95%"><span style="font-family:"B Zar""><span style="color:black"></span></span></span></span></span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:95%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><span lang="AR-SA" style="line-height:95%"><span style="font-family:"B Koodak""><span style="color:black"><span style="letter-spacing:-.2pt">یافته</span></span></span></span><span lang="FA" style="line-height:95%"><span style="font-family:"B Zar""><span style="color:black"><span style="letter-spacing:-.2pt"></span></span></span></span><span lang="AR-SA" style="line-height:95%"><span style="font-family:"B Koodak""><span style="color:black"><span style="letter-spacing:-.2pt">ها:</span></span></span></span> <span lang="FA" style="line-height:95%"><span style="font-family:"B Zar""><span style="color:black">تجویز </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:95%"><span style="color:black">EB</span></span></span><span style="line-height:95%"><span style="font-family:"B Zar""><span style="color:black"> باعث برهم خوردن غلظت طبیعی محتوای اسیدهای چرب اشباع نشده چندگانه، افزایش مشتقات اسید آراشیدونیک و کاهش اسید چرب دوکوزاهگزانوئیک اسید در نخاع موش</span></span></span><span lang="AR-SA" style="line-height:95%"><span style="font-family:"B Zar""></span></span><span lang="FA" style="line-height:95%"><span style="font-family:"B Zar""><span style="color:black">ها شد. درمان با اسانس سیاه دانه به صورت معنی دار(01/0</span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:95%"><span style="color:black">P<</span></span></span><span lang="FA" style="line-height:95%"><span style="font-family:"B Zar""><span style="color:black">) و وابسته به دوز از بروز این تغییرات ممانعت کرد.</span></span></span><span lang="FA" style="line-height:95%"><span style="font-family:"B Zar""><span style="color:black"></span></span></span></span></span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:95%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><span lang="AR-SA" style="line-height:95%"><span style="font-family:"B Koodak""><span style="color:black"><span style="letter-spacing:-.2pt">استنتاج:</span></span></span></span> <span lang="FA" style="line-height:95%"><span style="font-family:"B Zar""><span style="color:black">استفاده از اسانس </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:95%"><span style="color:black">NS</span></span></span><span style="line-height:95%"><span style="font-family:"B Zar""><span style="color:black"> سبب محافظت در مقابل دمیلیناسیون ناشی از </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:95%"><span style="color:black">EB</span></span></span><span lang="FA" style="line-height:95%"><span style="font-family:"B Zar""><span style="color:black"> از طریق تنظیم سطح اسیدهای چرب درون</span></span></span><span lang="AR-SA" style="line-height:95%"><span style="font-family:"B Zar""></span></span><span lang="FA" style="line-height:95%"><span style="font-family:"B Zar""><span style="color:black">زاد بافت نخاعی می</span></span></span><span lang="AR-SA" style="line-height:95%"><span style="font-family:"B Zar""></span></span><span lang="FA" style="line-height:95%"><span style="font-family:"B Zar""><span style="color:black">شود.</span></span></span><span lang="AR-SA" style="line-height:95%"><span style="font-family:"B Zar""><span style="color:black"><span style="letter-spacing:-.2pt"></span></span></span></span></span></span></span></span></span></span></span>
<span style="font-size:12pt"><span style="line-height:150%"><span style="font-family:"Times New Roman","serif""><b><span style="font-size:10.0pt"><span style="line-height:150%"><span style="font-family:"Arial","sans-serif""><span style="letter-spacing:-.2pt">Background and purpose:</span></span></span></span></b> <span style="font-size:10.0pt"><span style="line-height:150%"><span style="letter-spacing:-.2pt">Myelin degradation is one of the main causes of some neurological diseases. There is growing evidence that plant essential oils may reduce the risk of demyelination. </span></span></span><span lang="EN" style="font-size:10.0pt"><span style="line-height:150%">In this experimental study</span></span><span style="font-size:10.0pt"><span style="line-height:150%"><span style="letter-spacing:-.2pt">, using a lipidomics approach, we investigated whether administration of <i>Nigella sativa</i> (NS) essential oil could improve changes in the profile of spinal fatty acids in demyelination model induced by intra-spinal administration of ethidium bromide (EB) in mice.</span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:150%"><span style="text-autospace:none"><span style="font-family:"Times New Roman","serif""><b><span style="font-size:10.0pt"><span style="line-height:150%"><span style="font-family:"Arial","sans-serif"">Materials and methods:</span></span></span></b><b> </b><span style="font-size:10.0pt"><span style="line-height:150%"><span style="letter-spacing:-.2pt">In this experimental study, 30 male rats were studied in five groups (n=6). The groups included control without treatment, solvent, and black seed essential oil (2, 5, and 10 ml/kg, intraperitoneally). The essential oil of NS was prepared and its fatty acid composition was determined by gas chromatography-mass spectrometry (GC-MS). The animals underwent intra-spinal administration of ET 0.2%. At the end of the experiments, samples were taken from the spinal cord of the rats and the concentration of seven different types of fatty acids in the homogenate of the spinal cord was measured and compared between the groups.</span></span></span></span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="line-height:150%"><span style="font-family:"Times New Roman","serif""><b><span style="font-size:10.0pt"><span style="line-height:150%"><span style="font-family:"Arial","sans-serif"">Results:</span></span></span></b><b> </b><span style="font-size:10.0pt"><span style="line-height:150%"><span style="letter-spacing:-.2pt">Ethidium bromide disturbed the normal concentration of polyunsaturated fatty acids, increased arachidonic acid derivatives, and decreased docosahexaenoic acid in rat spinal cord. Treatment with NS essential oil significantly prevented these changes in a dose-dependent manner (P<0.01).</span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="line-height:150%"><span style="vertical-align:top"><span style="font-family:"Times New Roman","serif""><b><span style="font-size:10.0pt"><span style="line-height:150%"><span style="font-family:"Arial","sans-serif"">Conclusion:</span></span></span></b><span style="font-size:10.0pt"><span style="line-height:150%"> <span style="letter-spacing:-.2pt">NS essential oil protects against EB-induced demyelination by regulating the level of endogenous fatty acids in spinal tissue.</span><i></i></span></span></span></span></span></span><br>
<br>
سیاه دانه, اتیدیوم بروماید, دمیلیناسیون, نخاع
Nigella sativa, ethidium bromide, demyelination, spinal cord
55
64
http://jmums.mazums.ac.ir/browse.php?a_code=A-10-6167-2&slc_lang=fa&sid=1
Nafieseh Sadat
Mirshafieyan
نقیسه السادات
میرشفیعیان
mir.nsm@gmail.com
1003194753284600149295
1003194753284600149295
No
MSc in Animal Physiology, School of Basic Sciences, Shahed University, Tehran, Iran
کارشناس ارشد زیست شناسی جانوری، گروه زیست شناسی، دانشکده علوم پایه، دانشگاه شاهد، تهران، ایران
Majid
Hassanpourezatti
مجید
حسن پورعزتی
hassanpour@shahed.ac.ir
1003194753284600149294
1003194753284600149294
Yes
Assistant Professor, Department of Biology, School of Basic Sciences, Shahed University, Tehran, Iran
استادیار، گروه زیست شناسی، دانشکده علوم پایه، دانشگاه شاهد، تهران، ایران
hossein
dehghan
حسین
دهقان
h.dehghan@shahed.ac.ir
1003194753284600149296
1003194753284600149296
No
Assistant Professor, Medicinal Plants Research Center, Shahed University, Tehran, Iran
استادیار، مرکز تحقیقات گیاهان دارویی، دانشگاه شاهد، تهران، ایران