fa بررسی اثر ساپونین بر میزان ادم مغزی در مدل جانوری سکته مغزی فيزيولوژي physiology پژوهشي-کامل Research(Original) <span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span sans-serif="" style="font-family:Calibri,"><span lang="FA" style="font-size:12.0pt"><span b="" koodak="" style="font-family:">&nbsp; &nbsp;</span></span></span></span></span></span></span><span style="font-size:12pt"><span style="line-height:95%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span lang="FA" style="line-height:95%"><span b="" koodak="" style="font-family:"><span style="color:black"><span style="letter-spacing:-.1pt">سابقه</span></span></span></span><span lang="AR-SA" style="line-height:95%"><span b="" koodak="" style="font-family:"><span style="color:black"><span style="letter-spacing:-.1pt"> و هدف:</span></span></span></span> <span lang="FA" style="line-height:95%"><span b="" style="font-family:" zar="">استرس اکسیداتیو و التهاب عصبی از اصلی</span></span><span lang="AR-SA" style="line-height:95%"><span b="" style="font-family:" zar="">‌</span></span><span lang="FA" style="line-height:95%"><span b="" style="font-family:" zar="">ترین عوامل ایجاد ایسکمی مغزی، می</span></span><span lang="AR-SA" style="line-height:95%"><span b="" style="font-family:" zar="">‌</span></span><span lang="FA" style="line-height:95%"><span b="" style="font-family:" zar="">باشد. ساپونین با ترکیبات استروئیدی یا تری</span></span><span lang="AR-SA" style="line-height:95%"><span b="" style="font-family:" zar="">‌</span></span><span lang="FA" style="line-height:95%"><span b="" style="font-family:" zar="">ترپنوئیدی همراه با یک بخش قندی، یک آنتی</span></span><span lang="AR-SA" style="line-height:95%"><span b="" style="font-family:" zar="">‌</span></span><span lang="FA" style="line-height:95%"><span b="" style="font-family:" zar="">اکسیدان قوی و ضد التهاب محسوب می</span></span><span lang="AR-SA" style="line-height:95%"><span b="" style="font-family:" zar="">‌</span></span><span lang="FA" style="line-height:95%"><span b="" style="font-family:" zar="">شود. این مطالعه با هدف بررسی اثر ساپونین بر میزان ادم مغزی، سطح بیان ژن </span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:95%">Bax</span></span><span lang="FA" style="line-height:95%"><span b="" style="font-family:" zar=""> و نقص</span></span><span lang="AR-SA" style="line-height:95%"><span b="" style="font-family:" zar="">‌</span></span><span lang="FA" style="line-height:95%"><span b="" style="font-family:" zar="">های نورولوژیکی حاصل از ایسکمی انجام گرفت.<span style="color:#0e101a"></span></span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:95%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span lang="AR-SA" style="line-height:95%"><span b="" koodak="" style="font-family:"><span style="color:black"><span style="letter-spacing:-.1pt">مواد و روش</span></span></span></span><span lang="FA" style="line-height:95%"><span b="" style="font-family:" zar=""><span style="color:black"><span style="letter-spacing:-.1pt">‌</span></span></span></span><span lang="AR-SA" style="line-height:95%"><span b="" koodak="" style="font-family:"><span style="color:black"><span style="letter-spacing:-.1pt">ها:</span></span></span></span> <span lang="FA" style="line-height:95%"><span b="" style="font-family:" zar="">در این مطالعه تجربی 24 سر موش صحرایی نر نژاد ویستار به 3گروه</span></span> <span lang="FA" style="line-height:95%"><span b="" style="font-family:" zar="">شم، سکته و سکته+ساپونین تقسیم شدند. موش</span></span><span lang="AR-SA" style="line-height:95%"><span b="" style="font-family:" zar="">‌</span></span><span lang="FA" style="line-height:95%"><span b="" style="font-family:" zar="">های صحرایی گروه سکته+ساپونین 30 دقیقه پیش از ایسکمی مغزی، ساپونین(</span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:95%">mg/kg</span></span><span lang="FA" style="line-height:95%"><span b="" style="font-family:" zar="">3) را با تزریق وریدی دریافت نمودند. هر گروه به دو زیر گروه ادم مغزی (5تایی) و بررسی سطح بیان ژن(3تایی) تقسیم‌بندی شدند. ایسکمی مغزی با مدل انسداد شریان مغزی- میانی القا شد. 24 ساعت بعد ارزیابی نقص</span></span><span lang="AR-SA" style="line-height:95%"><span b="" style="font-family:" zar="">‌</span></span><span lang="FA" style="line-height:95%"><span b="" style="font-family:" zar="">های نورولوژیک حاصل از ایسکمی (آزمون کروسکال والیس)، میزان ادم مغزی و سطح بیان ژن </span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:95%">Bax</span></span><span lang="FA" style="line-height:95%"><span b="" style="font-family:" zar=""> در بخش</span></span><span lang="AR-SA" style="line-height:95%"><span b="" style="font-family:" zar="">‌</span></span><span lang="FA" style="line-height:95%"><span b="" style="font-family:" zar="">های </span></span><span lang="AR-SA" style="line-height:95%"><span b="" style="font-family:" zar="">کورتکس،</span></span><span lang="FA" style="line-height:95%"><span b="" style="font-family:" zar="">کورتکس</span></span><span lang="AR-SA" style="line-height:95%"><span b="" style="font-family:" zar=""> پری فرم-آمیگدال، استریاتوم و هیپوکامپ ( آزمون </span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:95%">ANOVA</span></span><span style="line-height:95%"><span b="" style="font-family:" zar=""> یک‌طرفه)</span></span><span lang="FA" style="line-height:95%"><span b="" style="font-family:" zar=""> بررسی شد.<span style="color:black"></span></span></span></span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:95%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span lang="AR-SA" style="line-height:95%"><span b="" koodak="" style="font-family:"><span style="color:black"><span style="letter-spacing:-.2pt">یافته</span></span></span></span><span lang="FA" style="line-height:95%"><span b="" style="font-family:" zar=""><span style="color:black"><span style="letter-spacing:-.2pt">‌</span></span></span></span><span lang="AR-SA" style="line-height:95%"><span b="" koodak="" style="font-family:"><span style="color:black"><span style="letter-spacing:-.2pt">ها:</span></span></span></span> <span lang="FA" style="line-height:95%"><span b="" style="font-family:" zar="">نتایج این پژوهش کاهش معنی داری را در امتیاز نقص</span></span><span lang="AR-SA" style="line-height:95%"><span b="" style="font-family:" zar="">‌</span></span><span lang="FA" style="line-height:95%"><span b="" style="font-family:" zar="">های نورولوژیکی حاصل از ایسکمی، میزان<b> </b>ادم مغزی و سطح بیان ژن</span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:95%">Bax </span></span><span lang="FA" style="line-height:95%"><span b="" style="font-family:" zar="">&nbsp;در گروه تجربی سکته+ساپونین نشان داده است(0/05</span></span><span lang="FA" style="font-size:10.0pt"><span style="line-height:95%"><span b="" style="font-family:" zar="">></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:95%">P</span></span><span lang="FA" style="line-height:95%"><span b="" style="font-family:" zar="">).<span style="color:black"></span></span></span></span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:95%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span lang="AR-SA" style="line-height:95%"><span b="" koodak="" style="font-family:"><span style="color:black"><span style="letter-spacing:-.2pt">استنتاج:</span></span></span></span> <span lang="FA" style="line-height:95%"><span b="" style="font-family:" zar="">بر اساس یافته</span></span><span lang="AR-SA" style="line-height:95%"><span b="" style="font-family:" zar="">‌</span></span><span lang="FA" style="line-height:95%"><span b="" style="font-family:" zar="">های این مطالعه، پیش درمان با ساپونین در موش</span></span><span lang="AR-SA" style="line-height:95%"><span b="" style="font-family:" zar="">‌</span></span><span lang="FA" style="line-height:95%"><span b="" style="font-family:" zar="">های صحرایی متحمل ایسکمی- خونرسانی مجدد می تواند در کاهش آسیب</span></span><span lang="AR-SA" style="line-height:95%"><span b="" style="font-family:" zar="">‌</span></span><span lang="FA" style="line-height:95%"><span b="" style="font-family:" zar="">های حاصل از ایسکمی مغزی موثر باشد.</span></span><span lang="AR-SA" style="line-height:95%"><span b="" style="font-family:" zar=""><span style="color:black"><span style="letter-spacing:-.2pt"></span></span></span></span></span></span></span></span></span></span></span> <span style="font-size:12pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:10.0pt"><span style="line-height:150%"><span arial="" style="font-family:"><span style="letter-spacing:-.2pt">Background and purpose:</span></span></span></span></b> <span style="font-size:10.0pt"><span style="line-height:150%">Oxidative stress and neuroinflammation are the main causes of cerebral ischemia. Saponin with steroid or triterpenoid compounds with a sugar part has strong antioxidant and anti-inflammatory properties. The aim of this study was to evaluate the effect of saponin on cerebral edema,</span></span> <span style="font-size:10.0pt"><span style="line-height:150%">Bax gene expression level, and ischemic neurological defects.</span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:150%"><span style="text-autospace:none"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:10.0pt"><span style="line-height:150%"><span arial="" style="font-family:">Materials and methods:</span></span></span></b><b> </b><span style="font-size:10.0pt"><span style="line-height:150%">In this experimental study, 24 male Wistar rats were divided into three groups: sham, stroke, and stroke+saponin. Rats in the stroke+saponin group received intravenous injection of saponin (3mg/kg) 30 minutes before cerebral ischemia. Each group was divided into two subgroups: brain edema (n=5) and gene expression level investigation (n=3). Cerebral ischemia was induced by middle cerebral artery occlusion model. After 24 hrs, we investigated neurological deficits resulting from ischemia (Kruskal-Wallis test), the degree of brain edema, and Bax gene expression levels in the cortex, piriform cortex-amygdala, striatum, and hippocampus (one-way ANOVA).</span></span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:10.0pt"><span style="line-height:150%"><span arial="" style="font-family:">Results:</span></span></span></b><b> </b><span style="font-size:10.0pt"><span style="line-height:150%">Findings indicated a significant decrease in the score of neurological defects resulting from ischemia, the amount of cerebral edema, and the level of Bax gene expression in stroke+saponin group (P<0.05).</span></span></span></span></span><br> <span style="font-size:12pt"><span style="line-height:150%"><span style="vertical-align:top"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:10.0pt"><span style="line-height:150%"><span arial="" style="font-family:">Conclusion:</span></span></span></b> <span style="font-size:10.0pt"><span style="line-height:150%">This study showed that saponin pretreatment in rats with ischemia-reperfusion injury can be effective in reducing the damage caused by cerebral ischemia.</span></span><i><span style="font-size:10.0pt"><span style="line-height:150%"></span></span></i></span></span></span></span><br> <br> &nbsp; سکته مغزی, ادم مغزی, ساپونین, Bax stroke, cerebral edema, saponin, Bax 43 54 http://jmums.mazums.ac.ir/browse.php?a_code=A-11-15587-1&slc_lang=fa&sid=1 Elmira Shiri المیرا شیری Elmira.shiri@yahoo.com 1003194753284600149306 1003194753284600149306 No PhD Student in Animal Physiology, Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran دانشجوی دکتری فیزیولوژی جانوری، گروه زیست شناسی، واحد علوم و تحقیقات ، دانشگاه آزاد اسلامی، تهران، ایران Mohammad Reza Bigdeli محمدرضا بیگدلی Bigdelimohammadreza@yahoo.com 1003194753284600149307 1003194753284600149307 Yes Associate Professor, Department of Animal Sciences and Marine Biology, Shahid Beheshti University, Tehran, Iran . دانشیار، گروه علوم جانوری وزیست شناسی دریا، دانشگاه شهید بهشتی، تهران، ایران Akram Eidi اکرم عیدی biologysrb99@gmail.com 1003194753284600149308 1003194753284600149308 No Professor, Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran استاد، گروه زیست شناسی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران Seyed Pejman Mortazavi سید پژمان مرتضوی Sp.mortazavi@gmail.com 1003194753284600149309 1003194753284600149309 No Associate Professor, Department of Veterinary, Science and Research Branch, Islamic Azad University, Tehran, Iran دانشیار، گروه دامپزشکی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران