fa بررسی اثرات ضد میکروبی عصاره‌ها و فراکسیون‌های مختلف گیاه Marrubium parviflorum داروسازی Pharmacy پژوهشي-کامل Research(Original) <span style="font-size:12pt"><span style="line-height:94%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span lang="FA" style="line-height:94%"><span b="" koodak="" style="font-family:"><span style="color:black"><span style="letter-spacing:-.1pt">سابقه</span></span></span></span><span lang="AR-SA" style="line-height:94%"><span b="" koodak="" style="font-family:"><span style="color:black"><span style="letter-spacing:-.1pt"> و هدف:</span></span></span></span> <span lang="FA" style="line-height:94%"><span b="" style="font-family:" zar=""><span style="color:black">گیاهان جنس </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:94%"><span style="color:black">Marrubium</span></span></span><span lang="FA" style="line-height:94%"><span b="" style="font-family:" zar=""><span style="color:black"> از دیرباز به عنوان داروی سنتی در درمان بیماری</span></span></span><span lang="AR-BH" style="line-height:94%"><span b="" style="font-family:" zar="">&lrm;</span></span><span lang="FA" style="line-height:94%"><span b="" style="font-family:" zar=""><span style="color:black">ها استفاده می</span></span></span><span lang="AR-BH" style="line-height:94%"><span b="" style="font-family:" zar="">&lrm;</span></span><span lang="FA" style="line-height:94%"><span b="" style="font-family:" zar=""><span style="color:black">شدند. هدف از این مطالعه ارزیابی اثرات ضد میکروبی عصاره</span></span></span><span lang="AR-BH" style="line-height:94%"><span b="" style="font-family:" zar="">&lrm;</span></span><span lang="FA" style="line-height:94%"><span b="" style="font-family:" zar=""><span style="color:black">ها و فراکسیون</span></span></span><span lang="AR-BH" style="line-height:94%"><span b="" style="font-family:" zar="">&lrm;</span></span><span lang="FA" style="line-height:94%"><span b="" style="font-family:" zar=""><span style="color:black">های گیاه </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:94%"><span style="color:black">Marrubium parviflorum</span></span></span><span lang="FA" style="line-height:94%"><span b="" style="font-family:" zar=""><span style="color:black"> می</span></span></span><span lang="AR-BH" style="line-height:94%"><span b="" style="font-family:" zar="">&lrm;</span></span><span lang="FA" style="line-height:94%"><span b="" style="font-family:" zar=""><span style="color:black">باشد.</span></span></span><span lang="FA" style="line-height:94%"><span b="" style="font-family:" zar=""><span style="color:#0e101a"></span></span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:94%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span lang="AR-SA" style="line-height:94%"><span b="" koodak="" style="font-family:"><span style="color:black">مواد و روش</span></span></span><span lang="FA" style="line-height:94%"><span b="" style="font-family:" zar=""><span style="color:black">‌</span></span></span><span lang="AR-SA" style="line-height:94%"><span b="" koodak="" style="font-family:"><span style="color:black">ها:</span></span></span> <span lang="FA" style="line-height:94%"><span b="" style="font-family:" zar=""><span style="color:black"><span style="letter-spacing:-.2pt">در این مطالعه تجربی، عصاره</span></span></span></span><span lang="AR-BH" style="line-height:94%"><span b="" style="font-family:" zar=""><span style="letter-spacing:-.2pt">&lrm;</span></span></span><span lang="FA" style="line-height:94%"><span b="" style="font-family:" zar=""><span style="color:black"><span style="letter-spacing:-.2pt">گیری از اندام هوایی گیاه با روش سوکسله انجام گرفت. اثر ضدمیکروبی</span></span></span></span><span lang="FA" style="line-height:94%"><span b="" style="font-family:" zar=""><span style="color:black"> عصاره</span></span></span><span lang="AR-BH" style="line-height:94%"><span b="" style="font-family:" zar="">&lrm;</span></span><span lang="FA" style="line-height:94%"><span b="" style="font-family:" zar=""><span style="color:black">ها بر روی شش سویه باکتری گرم مثبت، چهارسویه باکتری گرم منفی و یک سویه قارچ مورد ارزیابی قرار گرفت. هم</span></span></span><span lang="AR-BH" style="line-height:94%"><span b="" style="font-family:" zar="">&lrm;</span></span><span lang="FA" style="line-height:94%"><span b="" style="font-family:" zar=""><span style="color:black">چنین عصاره</span></span></span><span lang="AR-BH" style="line-height:94%"><span b="" style="font-family:" zar="">&lrm;</span></span><span lang="FA" style="line-height:94%"><span b="" style="font-family:" zar=""><span style="color:black">های موثر با انواع روش</span></span></span><span lang="AR-BH" style="line-height:94%"><span b="" style="font-family:" zar="">&lrm;</span></span><span lang="FA" style="line-height:94%"><span b="" style="font-family:" zar=""><span style="color:black">های کروماتوگرافی فراکسیونه شدند و اثرات ضدمیکروبی آن ها نیز بررسی شد.</span></span></span></span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:94%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span lang="AR-SA" style="line-height:94%"><span b="" koodak="" style="font-family:"><span style="color:black"><span style="letter-spacing:-.2pt">یافته</span></span></span></span><span lang="FA" style="line-height:94%"><span b="" style="font-family:" zar=""><span style="color:black"><span style="letter-spacing:-.2pt">‌</span></span></span></span><span lang="AR-SA" style="line-height:94%"><span b="" koodak="" style="font-family:"><span style="color:black"><span style="letter-spacing:-.2pt">ها:</span></span></span></span> <span lang="FA" style="line-height:94%"><span b="" style="font-family:" zar=""><span style="color:black"><span style="letter-spacing:-.2pt">عصاره کلروفرمی گیاه </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:94%"><span style="color:black"><span style="letter-spacing:-.2pt">M.parviflorum</span></span></span></span><span style="line-height:94%"><span b="" style="font-family:" zar=""><span style="color:black"><span style="letter-spacing:-.2pt"> علیه باکتری‌های </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:94%"><span style="color:black"><span style="letter-spacing:-.2pt">Micrococcus luteus</span></span></span></span><span lang="FA" style="line-height:94%"><span b="" style="font-family:" zar=""><span style="color:black"><span style="letter-spacing:-.2pt">، </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:94%"><span style="color:black"><span style="letter-spacing:-.2pt">Staphylococcus epidermidis</span></span></span></span> <span lang="FA" style="line-height:94%"><span b="" style="font-family:" zar=""><span style="color:black">و </span></span></span>&nbsp;<span dir="LTR" style="font-size:10.0pt"><span style="line-height:94%"><span style="color:black">Bacillus</span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:94%"><span style="color:black"> cereus</span></span></span><span lang="FA" style="line-height:94%"><span b="" style="font-family:" zar=""><span style="color:black"> به ترتیب با قطر هاله عدم رشد (</span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:94%"><span style="color:black">DIZ</span></span></span><span lang="FA" style="line-height:94%"><span b="" style="font-family:" zar=""><span style="color:black">) 16/6، 14/6 و 11/6 میلی</span></span></span><span lang="AR-BH" style="line-height:94%"><span b="" style="font-family:" zar="">&lrm;</span></span><span lang="FA" style="line-height:94%"><span b="" style="font-family:" zar=""><span style="color:black">متر و همگی با</span></span></span> <span lang="FA" style="line-height:94%"><span b="" style="font-family:" zar=""><span style="color:black">حداقل غلظت باکتریسیدال (</span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:94%"><span style="color:black">MBC</span></span></span><span lang="FA" style="line-height:94%"><span b="" style="font-family:" zar=""><span style="color:black">)100 میلی</span></span></span><span lang="AR-BH" style="line-height:94%"><span b="" style="font-family:" zar="">&lrm;</span></span><span lang="FA" style="line-height:94%"><span b="" style="font-family:" zar=""><span style="color:black">گرم بر میلی</span></span></span><span lang="AR-BH" style="line-height:94%"><span b="" style="font-family:" zar="">&lrm;</span></span><span lang="FA" style="line-height:94%"><span b="" style="font-family:" zar=""><span style="color:black">لیتر موثر بود. <i>&nbsp;</i>عصاره متانولی نیز</span></span></span> <span lang="FA" style="line-height:94%"><span b="" style="font-family:" zar=""><span style="color:black">علیه باکتری </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:94%"><span style="color:black">Proteus mirabilis</span></span></span><span lang="FA" style="line-height:94%"><span b="" style="font-family:" zar=""><span style="color:black"> و </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:94%"><span style="color:black">Psudomonas aeruginosa</span></span></span><span lang="FA" style="line-height:94%"><span b="" style="font-family:" zar=""><span style="color:black"> اثرات ضد میکروبی قابل توجهی نشان دادند. دربین فراکسیون</span></span></span><span lang="AR-BH" style="line-height:94%"><span b="" style="font-family:" zar="">&lrm;</span></span><span lang="FA" style="line-height:94%"><span b="" style="font-family:" zar=""><span style="color:black">های حاصل، فراکسیون 10، 20، 40 و 60 درصد عصاره کلروفرمی و فراکسیون</span></span></span><span lang="AR-BH" style="line-height:94%"><span b="" style="font-family:" zar="">&lrm;</span></span><span lang="FA" style="line-height:94%"><span b="" style="font-family:" zar=""><span style="color:black">های 20، 40 درصد عصاره متانولی</span></span></span> <span lang="FA" style="line-height:94%"><span b="" style="font-family:" zar=""><span style="color:black">بیش ترین اثر ضدمیکروبی را ایجاد کردند. مقادیر </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:94%"><span style="color:black">MBC</span></span></span><span lang="FA" style="line-height:94%"><span b="" style="font-family:" zar=""><span style="color:black"> و </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:94%"><span style="color:black">DIZ</span></span></span><span lang="FA" style="line-height:94%"><span b="" style="font-family:" zar=""><span style="color:black"> در فراکسیون</span></span></span><span lang="AR-BH" style="line-height:94%"><span b="" style="font-family:" zar="">&lrm;ها</span></span><span lang="FA" style="line-height:94%"><span b="" style="font-family:" zar=""><span style="color:black">ای به</span></span></span><span lang="AR-BH" style="line-height:94%"><span b="" style="font-family:" zar="">&lrm;</span></span><span lang="FA" style="line-height:94%"><span b="" style="font-family:" zar=""><span style="color:black">دست آمده نشان</span></span></span><span lang="AR-BH" style="line-height:94%"><span b="" style="font-family:" zar="">&lrm;</span></span><span lang="FA" style="line-height:94%"><span b="" style="font-family:" zar=""><span style="color:black">دهنده تغلیظ ترکیبات ضدمیکروبی در برخی از فراکسیون ها و حساسیت بیش</span></span></span><span lang="AR-BH" style="line-height:94%"><span b="" style="font-family:" zar="">&lrm;</span></span><span lang="FA" style="line-height:94%"><span b="" style="font-family:" zar=""><span style="color:black">تر باکتری</span></span></span><span lang="AR-BH" style="line-height:94%"><span b="" style="font-family:" zar="">&lrm;</span></span><span lang="FA" style="line-height:94%"><span b="" style="font-family:" zar=""><span style="color:black">های گرم مثبت نسبت به باکتری</span></span></span><span lang="AR-BH" style="line-height:94%"><span b="" style="font-family:" zar="">&lrm;</span></span><span lang="FA" style="line-height:94%"><span b="" style="font-family:" zar=""><span style="color:black">های گرم منفی می</span></span></span><span lang="AR-BH" style="line-height:94%"><span b="" style="font-family:" zar="">&lrm;</span></span><span lang="FA" style="line-height:94%"><span b="" style="font-family:" zar=""><span style="color:black">باشد.</span></span></span></span></span></span></span></span></span></span><br> <span dir="RTL" lang="AR-SA" style="font-size:12.0pt"><span b="" koodak="" style="font-family:"><span style="color:black">استنتاج:</span></span></span> <span dir="RTL" lang="FA" style="font-size:12.0pt"><span b="" style="font-family:" zar=""><span style="color:black">از بین تمامی عصاره</span></span></span><span dir="RTL" lang="AR-BH" style="font-size:12.0pt"><span b="" style="font-family:" zar="">&lrm;</span></span><span dir="RTL" lang="FA" style="font-size:12.0pt"><span b="" style="font-family:" zar=""><span style="color:black">های مطالعه شده، عصاره کلروفرمی و متانولی گیاه </span></span></span><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">M.parviflorum</span></span></span><i> </i><span dir="RTL" lang="FA" style="font-size:12.0pt"><span b="" style="font-family:" zar=""><span style="color:black">قوی</span></span></span><span dir="RTL" lang="AR-BH" style="font-size:12.0pt"><span b="" style="font-family:" zar="">&lrm;</span></span><span dir="RTL" lang="FA" style="font-size:12.0pt"><span b="" style="font-family:" zar=""><span style="color:black">ترین اثر ضد میکروبی را از خود نشان دادند و حساس‌ترین میکروارگانیسم </span></span></span><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">Micrococcus luteus</span></span></span><span dir="RTL" lang="FA" style="font-size:12.0pt"><span b="" style="font-family:" zar=""><span style="color:black"> بود.</span></span></span><br> &nbsp; <span style="font-size:12pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:10.0pt"><span style="line-height:150%"><span arial="" style="font-family:"><span style="letter-spacing:-.2pt">Background and purpose:</span></span></span></span></b> <a name="_Hlk119886337"><i><span style="font-size:10.0pt"><span style="line-height:150%">Marrubium</span></span></i></a><span style="font-size:10.0pt"><span style="line-height:150%"> plants have long been used as a traditional medicine in treatment of diseases. The aim of this study was to evaluate the antimicrobial effects of <i>Marrubium parviflorum</i> extracts and fractions.</span></span><span style="font-size:10.0pt"><span style="line-height:150%"></span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:150%"><span style="text-autospace:none"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:10.0pt"><span style="line-height:150%"><span arial="" style="font-family:">Materials and methods:</span></span></span></b><b> </b><span lang="EN" style="font-size:10.0pt"><span style="line-height:150%">In this experimental study, </span></span><span style="font-size:10.0pt"><span style="line-height:150%">solvent extraction from aerial parts of the plant was done by Soxhlet method. The antimicrobial effect of the extracts was evaluated on six strains of gram-positive bacteria, four strains of gram-negative bacteria, and one strain of fungus. Also, effective extracts were fractionated by various chromatographic methods and their antimicrobial effects were investigated.</span></span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:10.0pt"><span style="line-height:150%"><span arial="" style="font-family:">Results:</span></span></span></b><b> </b><span lang="EN" style="font-size:10.0pt"><span style="line-height:150%">Chloroform extract of <i>M. parviflorum</i> was found to be effective against <i>Micrococcus luteus</i>, <i>Staphylococcus epidermidis</i>, and <i>Bacillus cereus</i> with a growth inhibition zone diameter (DIZ) of 16.6, 14.6, and 11.6 mm, respectively, and MBC 100 mg/ml. Methanolic extract also showed considerable antimicrobial effects against <i>Proteus mirabilis </i>and <i>Psudomonas aeruginosa</i>. Among the fractions obtained, 10%, 20%, 40%, and 60% fractions of the chloroform extract and 20% and 40% fractions of the methanolic extract showed the highest antimicrobial effect. The superior values ​​of MBC and DIZ in fractions indicate the condensation of certain antimicrobial compounds in some fractions and higher sensitivity of gram-positive bacteria compared with gram-negative bacteria.</span></span></span></span></span><br> <span style="font-size:12pt"><span style="line-height:150%"><span style="vertical-align:top"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:10.0pt"><span style="line-height:150%"><span arial="" style="font-family:">Conclusion:</span></span></span></b> <span style="font-size:10.0pt"><span style="line-height:150%">Among the extracts studied, the chloroform and the methanolic extracts of <i>M. parviflorum</i> exhibited the strongest antimicrobial effect and <i>Micrococcus luteus</i> was the most sensitive microorganism.</span></span><i><span style="font-size:10.0pt"><span style="line-height:150%"><span style="color:black"></span></span></span></i></span></span></span></span><br> <br> &nbsp; Marrubium parviflorum, عصاره, فراکسیون, اثرضدمیکروبی, حداقل غلظت باکتریسیدال Marrubium parviflorum, extract, fraction, antimicrobial effect, minimum bactericidal concentration 35 48 http://jmums.mazums.ac.ir/browse.php?a_code=A-10-15659-1&slc_lang=fa&sid=1 Sanaz Hamedeyazdan ساناز حامدیزدان hamedeyazdans@hotmail.co.uk 1003194753284600150123 1003194753284600150123 Yes Associate Professor, Department of Pharmaceutical and Food Control, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran دانشیار، گروه کنترل دارو و غذا، دانشکده داروسازی، دانشگاه علوم پزشکی تبریز، تبریز، ایران Somayeh Hallaj-Nezhadi سمیه حلاج نژادی hallajnezhadis@tbzmed.ac.ir 1003194753284600150124 1003194753284600150124 No Doctor of Pharmacy, Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran دکترای عمومی داروسازی، کمیته تحقیقات دانشجویی، دانشگاه علوم پزشکی تبریز، تبریز، ایران Forough Mohammadi فروغ محمدی sanaz_redox@yhaoo.com 1003194753284600150125 1003194753284600150125 No Doctor of Pharmacy, Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran دکترای عمومی داروسازی، کمیته تحقیقات دانشجویی، دانشگاه علوم پزشکی تبریز، تبریز، ایران Shadi Najjar-Sadeghi شادی نجارصادقی shadisadeghi48@gmail.com 1003194753284600150126 1003194753284600150126 No Associate Professor, Biotechnology Research Center, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran دانشیار، مرکز تحقیقات بیوتکنولوژی (زیست فناوری) و دانشکده داروسازی، دانشگاه علوم پزشکی تبریز، تبریز، ایران