fa بررسی اثرات نسفاتین-1 روی نفوذپذیری سد خونی-مغزی، نمره نورولوژیکی و ادم مغزی به دنبال القای ترومای مغزی در موش صحرایی نر: یک مطالعه رفتاری و بیوشیمیایی فيزيولوژي physiology پژوهشي-کامل Research(Original) <span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:92%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span lang="FA" style="line-height:92%"><span b="" koodak="" style="font-family:"><span style="color:black"><span style="letter-spacing:-.1pt">سابقه</span></span></span></span><span lang="AR-SA" style="line-height:92%"><span b="" koodak="" style="font-family:"><span style="color:black"><span style="letter-spacing:-.1pt"> و هدف:</span></span></span></span> <span lang="FA" style="line-height:92%"><span b="" style="font-family:" zar="">آسیب مغزی ناشی از تروما (</span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%">TBI</span></span><span lang="FA" style="line-height:92%"><span b="" style="font-family:" zar="">) یکی از پیچیده</span></span><span lang="AR-SA" style="line-height:92%"><span b="" style="font-family:" zar="">‌</span></span><span lang="FA" style="line-height:92%"><span b="" style="font-family:" zar="">ترین بیماری</span></span><span lang="AR-SA" style="line-height:92%"><span b="" style="font-family:" zar="">‌</span></span><span lang="FA" style="line-height:92%"><span b="" style="font-family:" zar="">های شناخته شده درسیستم عصبی مرکزی می</span></span><span lang="AR-SA" style="line-height:92%"><span b="" style="font-family:" zar="">‌</span></span><span lang="FA" style="line-height:92%"><span b="" style="font-family:" zar="">باشد. نسفاتین یک پلیپپتید 82-آمینو اسید است که بر سیستم عصبی مرکزی موثر است.</span></span> <span lang="FA" style="line-height:92%"><span b="" style="font-family:" zar="">در این پژوهش نقش محافظت نورونی نسفاتین در فرایند ضربه مغزی منتشر در موش صحرایی و هم‌چنین تاثیر آن روی میزان ماتریکس متالوپروتئیناز-9 (</span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%">MMP-9</span></span><span lang="FA" style="line-height:92%"><span b="" style="font-family:" zar="">) بررسی شد.<span style="color:#0e101a"></span></span></span></span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:92%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span lang="AR-SA" style="line-height:92%"><span b="" koodak="" style="font-family:"><span style="color:black"><span style="letter-spacing:-.1pt">مواد و روش</span></span></span></span><span lang="FA" style="line-height:92%"><span b="" style="font-family:" zar=""><span style="color:black"><span style="letter-spacing:-.1pt">‌</span></span></span></span><span lang="AR-SA" style="line-height:92%"><span b="" koodak="" style="font-family:"><span style="color:black"><span style="letter-spacing:-.1pt">ها:</span></span></span></span> <span lang="FA" style="line-height:92%"><span b="" style="font-family:" zar="">در این مطالعه تجربی، 56 موش صحرایی نر</span></span> <span lang="FA" style="line-height:92%"><span b="" style="font-family:" zar="">نژاد ویستار به روش مارمارو تحت </span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%">TBI</span></span><span lang="FA" style="line-height:92%"><span b="" style="font-family:" zar=""> قرار گرفتند و 30 دقیقه بعد نسفاتین با دوزهای مختلف به صورت داخل صفاقی تزریق و در روز دوم و سوم تکرار شد. در زمان</span></span><span lang="AR-SA" style="line-height:92%"><span b="" style="font-family:" zar="">‌</span></span><span lang="FA" style="line-height:92%"><span b="" style="font-family:" zar="">های قبل از ضربه، بلافاصله پس از به هوش آمدن پس از القا ضربه، 24، 48 و 72 ساعت بعد از ضربه، </span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%">Veterinary Coma Scale</span></span><span lang="FA" style="line-height:92%"><span b="" style="font-family:" zar=""> و تست</span></span><span lang="AR-SA" style="line-height:92%"><span b="" style="font-family:" zar="">‌</span></span><span lang="FA" style="line-height:92%"><span b="" style="font-family:" zar="">های حرکتی و تعادلی </span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%">Beam Walk</span></span><span lang="FA" style="line-height:92%"><span b="" style="font-family:" zar=""> و </span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%">Beam Balance</span></span><span lang="FA" style="line-height:92%"><span b="" style="font-family:" zar=""> در رت</span></span><span lang="AR-SA" style="line-height:92%"><span b="" style="font-family:" zar="">‌</span></span><span lang="FA" style="line-height:92%"><span b="" style="font-family:" zar="">ها ثبت شد. پس از 72 ساعت،</span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%">CSF</span></span><span lang="FA" style="line-height:92%"><span b="" style="font-family:" zar=""> از سیسترنا مگنا جمع</span></span><span lang="AR-SA" style="line-height:92%"><span b="" style="font-family:" zar="">‌</span></span><span lang="FA" style="line-height:92%"><span b="" style="font-family:" zar="">آوری و جهت انجام آزمایش الایزا برای بررسی ماتریکس متالوپروتئیناز-9 استفاده شد.<span style="color:black"></span></span></span></span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:92%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span lang="AR-SA" style="line-height:92%"><span b="" koodak="" style="font-family:"><span style="color:black"><span style="letter-spacing:-.2pt">یافته</span></span></span></span><span lang="FA" style="line-height:92%"><span b="" style="font-family:" zar=""><span style="color:black"><span style="letter-spacing:-.2pt">‌</span></span></span></span><span lang="AR-SA" style="line-height:92%"><span b="" koodak="" style="font-family:"><span style="color:black"><span style="letter-spacing:-.2pt">ها:</span></span></span></span> <span lang="FA" style="line-height:92%"><span b="" style="font-family:" zar=""><span style="color:black">تجویز داخل صفاقی نسفاتین در دوزهای0 5 و </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="color:black">&micro;g/kg</span></span></span><span style="line-height:92%"><span b="" style="font-family:" zar=""><span style="color:black"> 10 می‌تواند باعث کاهش ادم مغزی، تخریب سد خونی مغزی و نمرات عصبی و تعادلی حرکتی(0/0001</span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="color:black">P<</span></span></span><span lang="FA" style="line-height:92%"><span b="" style="font-family:" zar=""><span style="color:black">) در مقایسه با گروه کنترل (شم و سالم) شود(0/001</span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="color:black">P<</span></span></span><span lang="FA" style="line-height:92%"><span b="" style="font-family:" zar=""><span style="color:black">). لازم به ذکر است که نسفاتین در دوز </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="color:black">&micro;g/kg</span></span></span><span style="line-height:92%"><span b="" style="font-family:" zar=""><span style="color:black"> 20 مؤثر نبود(0/05</span></span></span><span lang="FA" style="font-size:10.0pt"><span style="line-height:92%"><span b="" style="font-family:" zar=""><span style="color:black">></span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="color:black">P</span></span></span><span lang="FA" style="line-height:92%"><span b="" style="font-family:" zar=""><span style="color:black">).</span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="color:black"></span></span></span></span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:92%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span lang="AR-SA" style="line-height:92%"><span b="" koodak="" style="font-family:"><span style="color:black"><span style="letter-spacing:-.2pt">استنتاج:</span></span></span></span> <span lang="FA" style="line-height:92%"><span b="" style="font-family:" zar=""><span style="color:black">نسفاتین توانسته است بر پیامدهای تروما تأثیر بگذارد و ادم مغزی را کاهش دهد، بهبود سد خونی مغزی و نمرات عصبی و تعادلی را تسریع بخشد. لازم به ذکر است احتمالاً بخشی از این اثرات محافظتی عصبی نسفاتین از طریق کاهش </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="color:black">MMP-9</span></span></span><span style="line-height:92%"><span b="" style="font-family:" zar=""><span style="color:black"> می</span></span></span><span lang="AR-SA" style="line-height:92%"><span b="" style="font-family:" zar="">‌</span></span><span lang="FA" style="line-height:92%"><span b="" style="font-family:" zar=""><span style="color:black">باشد.</span></span></span><span lang="AR-SA" style="line-height:92%"><span b="" style="font-family:" zar=""><span style="color:black"><span style="letter-spacing:-.2pt"></span></span></span></span></span></span></span></span></span></span></span><br> <br> &nbsp; <span style="font-size:12pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:10.0pt"><span style="line-height:150%"><span arial="" style="font-family:"><span style="letter-spacing:-.2pt">Background and purpose:</span></span></span></span></b> <span style="font-size:10.0pt"><span style="line-height:150%">Traumatic brain injury (TBI) is one of the most complex diseases of the central nervous system (CNS). Nesfatin is an 82-amino acid effective polypeptide in CNS. In this study, we investigated the role of nesfatin in neuron protection in the process of diffuse concussion in rats and also its effect on the level of matrix metalloproteinase-9.</span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:150%"><span style="text-autospace:none"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:10.0pt"><span style="line-height:150%"><span arial="" style="font-family:">Materials and methods:</span></span></span></b><b> </b><span lang="EN" style="font-size:10.0pt"><span style="line-height:150%">In this experimental study, </span></span><span style="font-size:10.0pt"><span style="line-height:150%">56 Wistar rats were subjected to TBI by the Marmaru method. After 30 minutes, nesfatin was injected intraperitoneally at different doses, and repeated on second and third days. The results of Veterinary Coma Scale, beam walk and beam balance <span style="letter-spacing:-.1pt">tests were recorded at Pre-TBI, immediately after TBI, 24, 48, and 72 hours after TBI. After 72 hours, CSF</span> <span style="letter-spacing:-.1pt">was collected from the cisterna magna and used for ELISA test to measure the matrix metalloproteinase-9.</span></span></span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:10.0pt"><span style="line-height:150%"><span arial="" style="font-family:">Results:</span></span></span></b><b> </b><span style="font-size:10.0pt"><span style="line-height:150%">Intraperitoneal administration of nesfatin at 50 &micro;g/kg and 10 &micro;g/kg can reduce cerebral edema, destruction of the blood-brain barrier, and neurological and balance-motor scores (P<0.0001) compared with the control group (Sham and Intact) (P<0.0001). Nesfatin was not found effective at 20&micro;g/kg (P>0.05).</span></span></span></span></span><br> <span style="font-size:12pt"><span style="line-height:150%"><span style="vertical-align:top"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:10.0pt"><span style="line-height:150%"><span arial="" style="font-family:">Conclusion:</span></span></span></b><span style="font-size:10.0pt"><span style="line-height:150%"> Nesfatin has been able to affect the consequences of trauma and reduce brain edema, accelerate the improvement of blood-brain barrier and neurological and balance scores. Probably part of these neuroprotective effects is through reduction of MMP-9.<i></i></span></span></span></span></span></span><br> <br> &nbsp; نسفاتین, نوروپروتکتیو, ترومای مغزی, ادم مغزی, سد خونی- مغزی, MMP-9 nesfatin, neuroprotective, brain trauma, cerebral edema, blood-brain barrier, MMP-9 1 15 http://jmums.mazums.ac.ir/browse.php?a_code=A-10-7594-7&slc_lang=fa&sid=1 Alireza Taghizadeh علیرضا تقی زاده alirezataghizadeh930@gmail.com 1003194753284600151534 1003194753284600151534 No Medical Student, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran دانشجوی پزشکی، دانشکده پزشکی، دانشگاه علوم پزشکی مازندران، ساری، ایران Forouzan Sadeghimahalli فروزان صادقی محلی sadeghi.f.ph@gmail.com 1003194753284600151535 1003194753284600151535 No Assistant Professor, Department of Physiology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran استادیار، گروه فیزیولوژی، دانشکده پزشکی، دانشگاه علوم پزشکی مازندران، ساری، ایران Ali Siahposht –Khachaki علی سیاه پشت خاچکی ak57n@yahoo.com 1003194753284600151536 1003194753284600151536 Yes Associate Professor, Department of Physiology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran دانشیار، گروه فیزیولوژی، دانشکده پزشکی، دانشگاه علوم پزشکی مازندران، ساری، ایران