Journal of Mazandaran University of Medical Sciences
مجله دانشگاه علوم پزشکی مازندران
J Mazandaran Univ Med Sci
Medical Sciences
http://jmums.mazums.ac.ir
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اثر حفاظتی اپی کاتچین بر زنده مانی سلول عصبی PC12
مواجه شده با داروی والپروئیک اسید
Protective Effect of Epicatechin on Survival of PC12 Neurons Exposed to Valproic Acid
سم شناسی
toxicology
پژوهشي-کامل
Research(Original)
<span style="font-size:11pt"><span style="line-height:150%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><span lang="FA" style="font-size:12.0pt"><span style="line-height:150%"><span b="" nazanin="" style="font-family:"> </span></span></span></b></span></span></span></span></span><span style="font-size:12pt"><span style="line-height:92%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span lang="FA" style="line-height:92%"><span b="" koodak="" style="font-family:"><span style="color:black"><span style="letter-spacing:-.1pt">سابقه</span></span></span></span><span lang="AR-SA" style="line-height:92%"><span b="" koodak="" style="font-family:"><span style="color:black"><span style="letter-spacing:-.1pt"> و هدف:</span></span></span></span> <span lang="AR-SA" style="line-height:92%"><span b="" style="font-family:" zar="">به خوبی ثابت شده است که داروی ضد تشنج والپروئیک اسید با ایجاد استرس اکسیداتیو در انسان و در تمام گونه</span></span><span lang="AR-SA" style="line-height:92%"><span b="" style="font-family:" zar=""></span></span><span lang="AR-SA" style="line-height:92%"><span b="" style="font-family:" zar="">های حیوانی مورد آزمایش، تراتوژنیک است. در این مطالعه، باتوجه به ترکیب شیمیایی کاتچینها، اثر محافظتی آن بر زنده مانی سلول</span></span><span lang="AR-SA" style="line-height:92%"><span b="" style="font-family:" zar=""></span></span><span lang="AR-SA" style="line-height:92%"><span b="" style="font-family:" zar="">های عصبی </span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%">PC12</span></span><b> </b><span lang="AR-SA" style="line-height:92%"><span b="" style="font-family:" zar="">مواجه شده با داروی والپروئیک اسید بررسی شد.</span></span><span lang="FA" style="line-height:92%"><span b="" style="font-family:" zar=""><span style="color:#0e101a"></span></span></span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:92%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span lang="AR-SA" style="line-height:92%"><span b="" koodak="" style="font-family:"><span style="color:black"><span style="letter-spacing:-.1pt">مواد و روش</span></span></span></span><span lang="FA" style="line-height:92%"><span b="" style="font-family:" zar=""><span style="color:black"><span style="letter-spacing:-.1pt"></span></span></span></span><span lang="AR-SA" style="line-height:92%"><span b="" koodak="" style="font-family:"><span style="color:black"><span style="letter-spacing:-.1pt">ها:</span></span></span></span> <span lang="AR-SA" style="line-height:92%"><span b="" style="font-family:" zar="">اثرات محافظتی اپی کاتچین </span></span><span lang="AR-SA" style="line-height:92%"><span b="" style="font-family:" zar="">(<span style="color:black">دوز 10-50-100-500-1000 میکروگرم بر میلیلیتر</span>) بر سمیت ناشی از داروی والپروئیک اسید بر بقا و زنده مانی سلول عصبی </span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%">PC12</span></span> <span lang="AR-SA" style="line-height:92%"><span b="" style="font-family:" zar="">توسط تست </span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%">MTT</span></span><span style="line-height:92%"><span b="" style="font-family:" zar=""> و تستهای استرس اکسیداتیو شامل (ارزیابی میزان </span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%">ROS</span></span><span lang="AR-SA" style="line-height:92%"><span b="" style="font-family:" zar="">، </span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%">SOD</span></span><span lang="AR-SA" style="line-height:92%"><span b="" style="font-family:" zar="">، </span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%">GPx</span></span><span lang="AR-SA" style="line-height:92%"><span b="" style="font-family:" zar=""> و پراکسیداسیون لیپیدی) بررسی شد.<b> </b>تمامی داده</span></span><span lang="AR-SA" style="line-height:92%"><span b="" style="font-family:" zar=""></span></span><span lang="AR-SA" style="line-height:92%"><span b="" style="font-family:" zar="">ها با استفاده از آنالیز</span></span> <span lang="AR-SA" style="line-height:92%"><span b="" style="font-family:" zar="">واریانس یک طرفه </span></span><span dir="LTR" lang="EN-CA" style="font-size:10.0pt"><span style="line-height:92%">one-way ANOVA</span></span> <span lang="AR-SA" style="line-height:92%"><span b="" style="font-family:" zar="">و </span></span><span lang="AR-SA" style="line-height:92%"><span b="" style="font-family:" zar="">تست </span></span><span dir="LTR" lang="EN-CA" style="font-size:10.0pt"><span style="line-height:92%">Tukey</span></span> <span lang="AR-SA" style="line-height:92%"><span b="" style="font-family:" zar="">توسط </span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%">GraphPad Prism.8</span></span> <span lang="AR-SA" style="line-height:92%"><span b="" style="font-family:" zar="">تجزیه و تحلیل شدند</span></span><span lang="AR-SA" style="line-height:92%"><span b="" style="font-family:" zar="">. </span></span><span lang="FA" style="line-height:92%"><span b="" style="font-family:" zar=""><span style="color:black"></span></span></span></span></span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:92%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span lang="AR-SA" style="line-height:92%"><span b="" koodak="" style="font-family:"><span style="color:black"><span style="letter-spacing:-.2pt">یافته</span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="color:black"><span style="letter-spacing:-.2pt"></span></span></span></span><span lang="AR-SA" style="line-height:92%"><span b="" koodak="" style="font-family:"><span style="color:black"><span style="letter-spacing:-.2pt">ها:</span></span></span></span> <span lang="AR-SA" style="line-height:92%"><span b="" style="font-family:" zar="">با توجه به نتایج، اپی کاتچین اثرات سیتوتوکسیک اسید والپروئیک را در غلظت</span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:" tahoma=""></span></span></span><span lang="AR-SA" style="line-height:92%"><span b="" style="font-family:" zar="">های 500 و 1000 میکروگرم بر میلیلیتر کاهش داد(</span></span><span lang="FA" style="line-height:92%"><span b="" style="font-family:" zar="">0/0001</span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:" tim="">P<</span></span></span><span lang="FA" style="line-height:92%"><span b="" style="font-family:" zar="">). اپی کتچین به </span></span><b><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"></span></span></b><span lang="AR-SA" style="line-height:92%"><span b="" style="font-family:" zar="">طور </span></span><span b="" style="font-family:" zar="">معنیداری </span><span lang="AR-SA" style="line-height:92%"><span b="" style="font-family:" zar="">استرس اکسیداتیو ناشی از والپروئیک اسید را از طریق کاهش تولید گونه</span></span><span lang="FA" style="line-height:92%"><span b="" style="font-family:" zar=""></span></span><span lang="AR-SA" style="line-height:92%"><span b="" style="font-family:" zar="">های فعال اکسیژن </span></span><span lang="FA" style="line-height:92%"><span b="" style="font-family:" zar="">(</span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:" tim="">ROS</span></span></span><span lang="FA" style="line-height:92%"><span b="" style="font-family:" zar="">) </span></span><span lang="AR-SA" style="line-height:92%"><span b="" style="font-family:" zar="">و</span></span> <span lang="AR-SA" style="line-height:92%"><span b="" style="font-family:" zar="">مالون دی آلدهید </span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:" tim="">(MDA)</span></span></span><span lang="AR-SA" style="line-height:92%"><span b="" style="font-family:" zar=""> اعمال کرده است (0/001</span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:" tim=""><</span></span></span><span lang="FA" style="line-height:92%"><span b="" style="font-family:" zar=""> </span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:" tim="">P</span></span></span><span lang="FA" style="line-height:92%"><span b="" style="font-family:" zar="">و 0/0001</span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:" tim="">P<</span></span></span><span lang="FA" style="line-height:92%"><span b="" style="font-family:" zar="">).</span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"></span></span></span></span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:92%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span lang="AR-SA" style="line-height:92%"><span b="" koodak="" style="font-family:"><span style="letter-spacing:-.2pt">استنتاج:</span></span></span> <span lang="AR-SA" style="line-height:92%"><span b="" style="font-family:" zar="">مطالعه</span></span> <span lang="AR-SA" style="line-height:92%"><span b="" style="font-family:" zar="">حاضر نشان داد که مصرف اپیکاتچین می</span></span><span lang="AR-SA" style="line-height:92%"><span b="" style="font-family:" zar=""></span></span><span lang="AR-SA" style="line-height:92%"><span b="" style="font-family:" zar="">تواند پتانسیل آنتی</span></span><span lang="AR-SA" style="line-height:92%"><span b="" style="font-family:" zar=""></span></span><span lang="AR-SA" style="line-height:92%"><span b="" style="font-family:" zar="">اکسیدانی را افزایش دهد. همچنین، </span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%">EC</span></span><span style="line-height:92%"><span b="" style="font-family:" zar=""> محافظت سلولی قابل توجهی را در برابر سمیت اسید والپروئیک در سلولهای </span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%">PC12</span></span><span style="line-height:92%"><span b="" style="font-family:" zar=""> نشان داد.<span style="color:black"><span style="letter-spacing:-.2pt"></span></span></span></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="unicode-bidi:embed"><span style="line-height:107%"><span style="font-family:Calibri,sans-serif"><b><span style="line-height:107%"><span new="" roman="" style="font-family:" times=""> </span></span></b></span></span></span></span><span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:150%"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:10.0pt"><span style="line-height:150%"><span arial="" style="font-family:">Background and purpose:</span></span></span></b><b> </b><span style="font-size:10.0pt"><span style="line-height:150%">It is well established that valproic acid (VPA) is teratogenic associated with oxidative stress in humans and in all animal species tested. In this study, considering the chemical composition of catechins, we investigated its protective effect on the survival of PC12 nerve cells exposed to valproic acid</span></span><span style="font-size:10.0pt"><span style="background:#eeffde"><span style="line-height:150%"><span style="font-family:" vazir="">.</span></span></span></span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:150%"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:10.0pt"><span style="line-height:150%"><span arial="" style="font-family:">Materials and methods:</span></span></span></b><span style="font-size:10.0pt"><span style="line-height:150%"> The protective effects of epicatechin (EC) at different concentrations (10, 50, 100, 500, 1000 μg/ml) on survival and viability of PC12 neuronal cells exposed to valproic acid were measured by MTT assay and oxidative stress tests (GPx, SOD, ROS, and lipid peroxidation). All data were analyzed in GraphPad Prism 8.0 using one-way ANOVA and Tukey post-test.</span></span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:150%"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:10.0pt"><span style="line-height:150%"><span arial="" style="font-family:">Results:</span></span></span></b><span style="font-size:10.0pt"><span style="line-height:150%"> According to findings, EC reduced the cytotoxic effects of valproic acid at 500 and 1000 µg/ml (P<0.0001). EC significantly reduced the oxidative stress induced by</span></span> <span style="font-size:10.0pt"><span style="line-height:150%">valproic acid via decreasing reactive oxygen species (ROS)</span></span> <span style="font-size:10.0pt"><span style="line-height:150%">and malondialdehyde (MDA) production (P<0.001 and P<0.0001, respectively).</span></span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:150%"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:10.0pt"><span style="line-height:150%"><span arial="" style="font-family:">Conclusion:</span></span></span></b><b> </b><span style="font-size:10.0pt"><span style="line-height:150%">The present study showed that epicatechin could increase antioxidant potential and exhibits significant cytoprotective effect against valproic acid toxicity in PC12 cells.</span></span></span></span></span></span></span><br>
استرس اکسیداتیو, اپی کاتچین, والپروئیک اسید, PC12
oxidative stress, epicatechin, valproic acid, PC12
31
42
http://jmums.mazums.ac.ir/browse.php?a_code=A-11-14144-3&slc_lang=fa&sid=1
Mohammad
Shokrzadeh
محمد
شکرزاده
mslamuki@gmail.com
1003194753284600154150
1003194753284600154150
No
Professor, Department of Toxicology and Pharmacology, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran
استاد، گروه سم شناسی و داروشناسی، دانشکده داروسازی، دانشگاه علوم پزشکی مازندران، ساری، ایران
Abbas
Mohammad pour
عباس
محمدپور
abbasbiology@yahoo.com
1003194753284600154151
1003194753284600154151
No
Assistant Professor, Department of Pharmacology and Toxicology, Faculty of Pharmacy, Sana University, Sari, Iran
استادیار، گروه سم شناسی و داروشناسی، دانشکده داروسازی، دانشگاه غیر انتفاعی سنا، ساری، ایران
Zahra
Tajik
زهرا
تاجیک
tajikzahra20@gmail.com
1003194753284600154152
1003194753284600154152
No
MSc Student in Genetics, Faculty of Pharmacy, Sana University, Sari, Iran
دانشجوی کارشناسی ارشد زیست شناسی ژنتیک، گروه سم شناسی و داروشناسی، دانشکده داروسازی، دانشگاه غیر انتفاعی سنا، ساری، ایران
Maryam
Alizadeh
مریم
علیزاده
1003194753284600154153
1003194753284600154153
No
MSc in Applied Chemistry, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran
کارشناسی ارشد شیمی کاربردی، دانشکده داروسازی، دانشگاه علوم پزشکی مازندران، ساری، ایران
Shaghayegh
Aghajanshakeri
شقایق
آقاجان شاکری
Shaghayegh.aghajanshakeri@gmail.com
1003194753284600154154
1003194753284600154154
Yes
PhD in Toxicology, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran
دکترای تخصصی سم شناسی، دانشکده داروسازی، دانشگاه علوم پزشکی مازندران، ساری، ایران