Journal of Mazandaran University of Medical Sciences
مجله دانشگاه علوم پزشکی مازندران
J Mazandaran Univ Med Sci
Medical Sciences
http://jmums.mazums.ac.ir
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بررسی تراکم ماست سلها و عروق ریز خونی و ارتباط آنها در
ژانت سل گرانولومای محیطی حفره دهان
Correlation between Mast Cell Counts and Microvessel Density in Peripheral Giant Cell Granuloma of the Oral Cavity
دندانپزشکی
Dentistry
پژوهشي-کامل
Research(Original)
<span style="font-size:12pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span b="" style="font-family:" zar=""><span style="color:#0d0d0d"> </span></span></span></span></span><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:95%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><span lang="FA" style="line-height:95%"><span style="font-family:"B Koodak""><span style="color:black"><span style="letter-spacing:-.1pt">سابقه</span></span></span></span><span lang="AR-SA" style="line-height:95%"><span style="font-family:"B Koodak""><span style="color:black"><span style="letter-spacing:-.1pt"> و هدف:</span></span></span></span> <span lang="FA" style="line-height:95%"><span style="font-family:"B Zar""><span style="color:#0d0d0d">نقش قطعی ماست سل</span></span></span><span lang="AR-SA" style="line-height:95%"><span style="font-family:"B Zar""><span style="color:black"></span></span></span><span lang="FA" style="line-height:95%"><span style="font-family:"B Zar""><span style="color:#0d0d0d">ها و آنژیوژنزیس در پاتوژنز ژانت سل گرانولومای محیطی (</span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:95%"><span style="color:#0d0d0d">PGCG</span></span></span><span lang="FA" style="line-height:95%"><span style="font-family:"B Zar""><span style="color:#0d0d0d">)</span></span></span> <span lang="FA" style="line-height:95%"><span style="font-family:"B Zar""><span style="color:#0d0d0d">حفره دهان هنوز به</span></span></span><span lang="AR-SA" style="line-height:95%"><span style="font-family:"B Zar""><span style="color:black"></span></span></span><span lang="FA" style="line-height:95%"><span style="font-family:"B Zar""><span style="color:#0d0d0d">طور کامل مشخص نشده است. لذا هدف از انجام این مطالعه، بررسی تعداد ماستسل</span></span></span><span lang="AR-SA" style="line-height:95%"><span style="font-family:"B Zar""><span style="color:black"></span></span></span><span lang="FA" style="line-height:95%"><span style="font-family:"B Zar""><span style="color:#0d0d0d">ها (</span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:95%"><span style="color:#0d0d0d">MCs</span></span></span><span lang="FA" style="line-height:95%"><span style="font-family:"B Zar""><span style="color:#0d0d0d">) و متوسط تراکم عروقی (</span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:95%"><span style="color:#0d0d0d">MVD</span></span></span><span lang="FA" style="line-height:95%"><span style="font-family:"B Zar""><span style="color:#0d0d0d">)</span></span></span> <span lang="FA" style="line-height:95%"><span style="font-family:"B Zar""><span style="color:#0d0d0d">و تعیین ارتباط بین آن</span></span></span><span lang="AR-SA" style="line-height:95%"><span style="font-family:"B Zar""><span style="color:black"></span></span></span><span lang="FA" style="line-height:95%"><span style="font-family:"B Zar""><span style="color:#0d0d0d">ها در </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:95%"><span style="color:#0d0d0d">PGCG</span></span></span><span lang="FA" style="line-height:95%"><span style="font-family:"B Zar""><span style="color:#0d0d0d"> حفره دهان می باشد.</span></span></span><span lang="FA" style="line-height:95%"><span style="font-family:"B Zar""><span style="color:#0e101a"></span></span></span></span></span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:95%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><span lang="AR-SA" style="line-height:95%"><span style="font-family:"B Koodak""><span style="color:black"><span style="letter-spacing:-.1pt">مواد و روش</span></span></span></span><span lang="FA" style="line-height:95%"><span style="font-family:"B Zar""><span style="color:black"><span style="letter-spacing:-.1pt"></span></span></span></span><span lang="AR-SA" style="line-height:95%"><span style="font-family:"B Koodak""><span style="color:black"><span style="letter-spacing:-.1pt">ها:</span></span></span></span> <span lang="FA" style="line-height:95%"><span style="font-family:"B Zar""><span style="color:#0d0d0d">در این مطالعه توصیفی- تحلیلی، تعداد 50 نمونه بافتی از آرشیو بخش پاتولوژی دهان دانشکده <span style="letter-spacing:-.1pt">دندانپزشکی زاهدان در طی سال</span></span></span></span><span lang="AR-SA" style="line-height:95%"><span style="font-family:"B Zar""><span style="color:black"><span style="letter-spacing:-.1pt"></span></span></span></span><span lang="FA" style="line-height:95%"><span style="font-family:"B Zar""><span style="color:#0d0d0d"><span style="letter-spacing:-.1pt">های 2015- 2005میلادی به روش سرشماری انتخاب شدند. نمونه</span></span></span></span><span lang="AR-SA" style="line-height:95%"><span style="font-family:"B Zar""><span style="color:black"><span style="letter-spacing:-.1pt"></span></span></span></span><span lang="FA" style="line-height:95%"><span style="font-family:"B Zar""><span style="color:#0d0d0d"><span style="letter-spacing:-.1pt">ها شامل: 40 مورد </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:95%"><span style="color:#0d0d0d"><span style="letter-spacing:-.1pt">PGCG</span></span></span></span><span style="line-height:95%"><span style="font-family:"B Zar""><span style="color:#0d0d0d"> و 10 مورد بافت نرمال دهانی بودند. نمونه</span></span></span><span lang="AR-SA" style="line-height:95%"><span style="font-family:"B Zar""><span style="color:black"></span></span></span><span lang="FA" style="line-height:95%"><span style="font-family:"B Zar""><span style="color:#0d0d0d">ها با روش رنگ</span></span></span><span lang="AR-SA" style="line-height:95%"><span style="font-family:"B Zar""><span style="color:black"></span></span></span><span lang="FA" style="line-height:95%"><span style="font-family:"B Zar""><span style="color:#0d0d0d">آمیزی ایمونوهیستوشیمی (آنتی</span></span></span><span lang="AR-SA" style="line-height:95%"><span style="font-family:"B Zar""><span style="color:black"></span></span></span><span lang="FA" style="line-height:95%"><span style="font-family:"B Zar""><span style="color:#0d0d0d">بادی </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:95%"><span style="color:#0d0d0d">CD31</span></span></span><span lang="FA" style="line-height:95%"><span style="font-family:"B Zar""><span style="color:#0d0d0d">) و تولوئیدن بلو جهت شناسایی </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:95%"><span style="color:#0d0d0d">MVD</span></span></span><span lang="FA" style="line-height:95%"><span style="font-family:"B Zar""><span style="color:#0d0d0d"> و </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:95%"><span style="color:#0d0d0d">MCs</span></span></span><span lang="FA" style="line-height:95%"><span style="font-family:"B Zar""><span style="color:#0d0d0d"> به</span></span></span><span lang="AR-SA" style="line-height:95%"><span style="font-family:"B Zar""><span style="color:black"></span></span></span><span lang="FA" style="line-height:95%"><span style="font-family:"B Zar""><span style="color:#0d0d0d">ترتیب رنگ</span></span></span><span lang="AR-SA" style="line-height:95%"><span style="font-family:"B Zar""><span style="color:black"></span></span></span><span lang="FA" style="line-height:95%"><span style="font-family:"B Zar""><span style="color:#0d0d0d">آمیزی شدند. سپس آنالیز داده</span></span></span><span lang="AR-SA" style="line-height:95%"><span style="font-family:"B Zar""><span style="color:black"></span></span></span><span lang="FA" style="line-height:95%"><span style="font-family:"B Zar""><span style="color:#0d0d0d">ها توسط </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:95%"><span style="color:#0d0d0d">T-test</span></span></span><span lang="FA" style="line-height:95%"><span style="font-family:"B Zar""><span style="color:#0d0d0d">، آزمون همبستگی پیرسون و نرم</span></span></span><span lang="AR-SA" style="line-height:95%"><span style="font-family:"B Zar""><span style="color:black"></span></span></span><span lang="FA" style="line-height:95%"><span style="font-family:"B Zar""><span style="color:#0d0d0d">افزار آماری </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:95%"><span style="color:#0d0d0d">SPSS 21</span></span></span><span style="line-height:95%"><span style="font-family:"B Zar""><span style="color:#0d0d0d"> صورت پذیرفت.</span></span></span><span lang="FA" style="line-height:95%"><span style="font-family:"B Zar""><span style="color:black"></span></span></span></span></span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:95%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><span lang="AR-SA" style="line-height:95%"><span style="font-family:"B Koodak""><span style="color:black"><span style="letter-spacing:-.2pt">یافته</span></span></span></span><span lang="FA" style="line-height:95%"><span style="font-family:"B Zar""><span style="color:black"><span style="letter-spacing:-.2pt"></span></span></span></span><span lang="AR-SA" style="line-height:95%"><span style="font-family:"B Koodak""><span style="color:black"><span style="letter-spacing:-.2pt">ها:</span></span></span></span> <span lang="FA" style="line-height:95%"><span style="font-family:"B Zar""><span style="color:#0d0d0d">متوسط تعداد ماست سل</span></span></span><span lang="AR-SA" style="line-height:95%"><span style="font-family:"B Zar""><span style="color:black"></span></span></span><span lang="FA" style="line-height:95%"><span style="font-family:"B Zar""><span style="color:#0d0d0d">ها در </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:95%"><span style="color:#0d0d0d">PGCG</span></span></span><span lang="FA" style="line-height:95%"><span style="font-family:"B Zar""><span style="color:#0d0d0d">، 36/43</span></span></span><span lang="FA" style="font-size:10.0pt"><span style="line-height:95%"><span style="color:#0d0d0d">±</span></span></span><span lang="FA" style="line-height:95%"><span style="font-family:"B Zar""><span style="color:#0d0d0d">38/51 و در بافت نرمال 21/3</span></span></span><span lang="FA" style="font-size:10.0pt"><span style="line-height:95%"><span style="color:#0d0d0d">±</span></span></span><span lang="FA" style="line-height:95%"><span style="font-family:"B Zar""><span style="color:#0d0d0d">14 بود و اختلاف معناداری را از لحاظ آماری نشان داد (0/041=</span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:95%"><span style="color:#0d0d0d">P</span></span></span><span lang="FA" style="line-height:95%"><span style="font-family:"B Zar""><span style="color:#0d0d0d">). </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:95%"><span style="color:#0d0d0d">MVD</span></span></span><span lang="FA" style="line-height:95%"><span style="font-family:"B Zar""><span style="color:#0d0d0d"> در گروه </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:95%"><span style="color:#0d0d0d">PGCG</span></span></span><span lang="FA" style="line-height:95%"><span style="font-family:"B Zar""><span style="color:#0d0d0d">، 64/43</span></span></span><span lang="FA" style="font-size:10.0pt"><span style="line-height:95%"><span style="color:#0d0d0d">±</span></span></span><span lang="FA" style="line-height:95%"><span style="font-family:"B Zar""><span style="color:#0d0d0d">93/34 و در گروه بافت نرمال، 16/7</span></span></span><span lang="FA" style="font-size:10.0pt"><span style="line-height:95%"><span style="color:#0d0d0d">±</span></span></span><span lang="FA" style="line-height:95%"><span style="font-family:"B Zar""><span style="color:#0d0d0d">41/9 بود و این اختلاف از لحاظ آماری معنی</span></span></span><span lang="AR-SA" style="line-height:95%"><span style="font-family:"B Zar""><span style="color:black"></span></span></span><span lang="FA" style="line-height:95%"><span style="font-family:"B Zar""><span style="color:#0d0d0d">دار بود(0/017=</span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:95%"><span style="color:#0d0d0d">P</span></span></span><span lang="FA" style="line-height:95%"><span style="font-family:"B Zar""><span style="color:#0d0d0d">). </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:95%"><span style="color:#0d0d0d">MVD</span></span></span><span lang="FA" style="line-height:95%"><span style="font-family:"B Zar""><span style="color:#0d0d0d"> و </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:95%"><span style="color:#0d0d0d">MCs</span></span></span><span lang="FA" style="line-height:95%"><span style="font-family:"B Zar""><span style="color:#0d0d0d"> در ناحیه زیر اپیتلیوم نسبت به نواحی عمقی</span></span></span><span lang="AR-SA" style="line-height:95%"><span style="font-family:"B Zar""><span style="color:black"></span></span></span><span lang="FA" style="line-height:95%"><span style="font-family:"B Zar""><span style="color:#0d0d0d">تر بافت همبند بیش</span></span></span><span lang="AR-SA" style="line-height:95%"><span style="font-family:"B Zar""><span style="color:black"></span></span></span><span lang="FA" style="line-height:95%"><span style="font-family:"B Zar""><span style="color:#0d0d0d">تر بود. بین </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:95%"><span style="color:#0d0d0d">MCs</span></span></span><span lang="FA" style="line-height:95%"><span style="font-family:"B Zar""><span style="color:#0d0d0d"> و </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:95%"><span style="color:#0d0d0d">MVD</span></span></span><span lang="FA" style="line-height:95%"><span style="font-family:"B Zar""><span style="color:#0d0d0d"> ارتباطی مشاهده نشد (0/003=</span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:95%"><span style="color:#0d0d0d">r</span></span></span><span lang="FA" style="line-height:95%"><span style="font-family:"B Zar""><span style="color:#0d0d0d">)(0/982=</span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:95%"><span style="color:#0d0d0d">P</span></span></span><span lang="FA" style="line-height:95%"><span style="font-family:"B Zar""><span style="color:#0d0d0d">).</span></span></span><span lang="FA" style="line-height:95%"><span style="font-family:"B Zar""><span style="color:black"></span></span></span></span></span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:95%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><span lang="AR-SA" style="line-height:95%"><span style="font-family:"B Koodak""><span style="color:black"><span style="letter-spacing:-.2pt">استنتاج:</span></span></span></span> <span lang="FA" style="line-height:95%"><span style="font-family:"B Zar""><span style="color:#0d0d0d">در مطالعه حاضر، تعداد ماست سل</span></span></span><span lang="AR-SA" style="line-height:95%"><span style="font-family:"B Zar""><span style="color:black"></span></span></span><span lang="FA" style="line-height:95%"><span style="font-family:"B Zar""><span style="color:#0d0d0d">ها و متوسط تراکم عروقی در </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:95%"><span style="color:#0d0d0d">PGCG</span></span></span><span lang="FA" style="line-height:95%"><span style="font-family:"B Zar""><span style="color:#0d0d0d"> بالاتر از بافت نرمال بود و این احتمالاً نقش ماست سل</span></span></span><span lang="AR-SA" style="line-height:95%"><span style="font-family:"B Zar""><span style="color:black"></span></span></span><span lang="FA" style="line-height:95%"><span style="font-family:"B Zar""><span style="color:#0d0d0d">ها و به دنبال آن روند آنژیوژنز را در پاتوژنز این ضایعه تحریکی حفره دهان نشان می</span></span></span><span lang="AR-SA" style="line-height:95%"><span style="font-family:"B Zar""><span style="color:black"></span></span></span><span lang="FA" style="line-height:95%"><span style="font-family:"B Zar""><span style="color:#0d0d0d">دهد، ولی بین تعداد ماست سل</span></span></span><span lang="AR-SA" style="line-height:95%"><span style="font-family:"B Zar""><span style="color:black"></span></span></span><span lang="FA" style="line-height:95%"><span style="font-family:"B Zar""><span style="color:#0d0d0d">ها و تراکم عروق خونی کوچک ارتباطی مشاهده نشد.</span></span></span><span lang="AR-SA" style="line-height:95%"><span style="font-family:"B Zar""><span style="color:black"><span style="letter-spacing:-.2pt"></span></span></span></span></span></span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="line-height:140%"><span new="" roman="" style="font-family:" times=""><span style="font-size:10.0pt"><span style="line-height:140%"><span style="color:#0d0d0d"><i> </i></span></span></span></span></span><span style="line-height:150%"><span style="font-family:"Times New Roman","serif""><b><span style="font-size:10.0pt"><span style="line-height:150%"><span style="font-family:"Arial","sans-serif""><span style="letter-spacing:-.2pt">Background and purpose:</span></span></span></span></b> <span style="font-size:10.0pt"><span style="line-height:150%">The definitive role of mast cells and angiogenesis in the pathogenesis of Peripheral giant cell granuloma (PGCG) of the oral cavity is not yet fully understood. The aim of this study was to evaluate the mast cell counts (MCs) and microvascular density (MVD) and investigating the relationship between them and PGCG of the oral cavity.</span></span></span></span></span><br>
<span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:150%"><span style="text-autospace:none"><span style="font-family:"Times New Roman","serif""><b><span style="font-size:10.0pt"><span style="line-height:150%"><span style="font-family:"Arial","sans-serif"">Materials and methods:</span></span></span></b><b> </b><span style="font-size:10.0pt"><span style="line-height:150%">In this descriptive-analytic study, 50 samples were selected from the archieve of oral pathology department in dental school at Zahedan University of Medical Sciences using census. The samples included 40 PGCG and 10 normal oral tissues that were stained by immunohistochemistry (CD31 antibody) and toluidine blue to identify MVD and MCs, respectively. Data were analyzed using T-test, Pearson correlation test in SPSS V.21.</span></span></span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="line-height:150%"><span style="font-family:"Times New Roman","serif""><b><span style="font-size:10.0pt"><span style="line-height:150%"><span style="font-family:"Arial","sans-serif"">Results</span></span></span></b><b><span style="font-size:10.0pt"><span style="line-height:150%">:</span></span></b><b> </b><span style="font-size:10.0pt"><span style="line-height:150%">The mean number of mast cells was 38.51±36.43 in PGCG and 14±3.21 in normal tissues indicating significant difference between the two groups (P= 0.041). Findings showed a significant difference in MVD between PGCG group (93.34±64.43) and normal tissues (41.9±16.7) (P=0.017). MVD and MCs below the epithelium were found to be higher compared with those in deeper area of connective tissue. No correlation was found between the MCs and MVD (r=0.003, P=0.982).</span></span></span></span></span><br>
<span style="font-size:12pt"><span style="line-height:150%"><span style="vertical-align:top"><span style="font-family:"Times New Roman","serif""><b><span style="font-size:10.0pt"><span style="line-height:150%"><span style="font-family:"Arial","sans-serif"">Conclusion</span></span></span></b><b><span style="font-size:10.0pt"><span style="line-height:150%">:</span></span></b><span style="font-size:10.0pt"><span style="line-height:150%"> In the present study, mast cells count and microvascular density were higher in PGCG than normal tissue which could explain the role of mast cells and the subsequent process of angiogenesis in the pathogenesis of this reactive lesion of the oral cavity,</span></span> <span style="font-size:10.0pt"><span style="line-height:150%">but no correlation was found between the mast cells count and microvascular density.<i></i></span></span></span></span></span></span><br>
ژانت سل گرانولومای محیطی, ماست سل, CD31
peripheral giant cell granuloma, mast cell, CD31
48
56
http://jmums.mazums.ac.ir/browse.php?a_code=A-10-15949-1&slc_lang=fa&sid=1
Hamideh
Kadeh
حمیده
کده
h_a_kade@yahoo.com
1003194753284600156835
1003194753284600156835
No
Associate Professor, Oral and Dental Disease Research Center, Department of Oral and Maxillofacial Pathology, School of Dentistry, Zahedan University of Medical Sciences, Zahedan, Iran
دانشیار، مرکز تحقیقات بیماری های دهان و دندان، گروه آسیب شناسی دهان، فک و صورت، دانشکده دندانپزشکی، زاهدان، ایران
Shirin
Saravani
شیرین
سراوانی
shirin.saravani@gmail.com
1003194753284600156836
1003194753284600156836
Yes
Associate Professor, Oral and Dental Disease Research Center, Department of Oral and Maxillofacial Pathology, School of Dentistry, Zahedan University of Medical Sciences, Zahedan, Iran
دانشیار، مرکز تحقیقات بیماری های دهان و دندان، گروه آسیب شناسی دهان، فک و صورت، دانشکده دندانپزشکی، زاهدان، ایران
Mahsa
Mohammadpour
مهسا
محمدپور
drmahsa.mm@gmail.com
1003194753284600156837
1003194753284600156837
No
Resident of Oral and Maxillofacial Radiology, School of Dentistry, Shahid Beheshti University of Medical Sciences, Tehran, Iran
دستیار تخصصی رادیولوژی دهان، فک و صورت، دانشکده دندانپزشکی، دانشگاه علوم پزشکی شهید بهشتی، تهران، ایران