Journal of Mazandaran University of Medical Sciences
مجله دانشگاه علوم پزشکی مازندران
J Mazandaran Univ Med Sci
Medical Sciences
http://jmums.mazums.ac.ir
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2023
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بررسی اثر تزریق هم زمان سلولهای اجدادی اندوتلیال و سلولهای بنیادی مزانشیمی در کاهش پاسخ التهابی سیستمیک ناشی از سپسیس القا شده با لیپوپلی ساکارید اشریشیاکولی در مدل موشی
Effect of Co-injection of Endothelial Progenitor Cells and Mesenchymal Stem Cells in Reducing Systemic Inflammatory Response in Sepsis Induced by Escherichia coli Lipopolysaccharide in Mouse Model
ايمونولوژي
Immunology
پژوهشي-کامل
Research(Original)
<span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:95%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span lang="FA" style="line-height:95%"><span b="" koodak="" style="font-family:"><span style="color:black"> </span></span></span></span></span></span></span></span><span style="font-size:12pt"><span style="line-height:92%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><span lang="FA" style="line-height:92%"><span style="font-family:"B Koodak""><span style="color:black"><span style="letter-spacing:-.1pt">سابقه</span></span></span></span><span lang="AR-SA" style="line-height:92%"><span style="font-family:"B Koodak""><span style="color:black"><span style="letter-spacing:-.1pt"> و هدف:</span></span></span></span> <span lang="FA" style="background:white"><span style="line-height:92%"><span style="font-family:"B Zar"">سلول‏های پیش ساز اندوتلیال (</span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="background:white"><span style="line-height:92%">EPC</span></span></span><span lang="FA" style="background:white"><span style="line-height:92%"><span style="font-family:"B Zar"">) و سلول‏های بنیادی مزانشیمی (</span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="background:white"><span style="line-height:92%">MSC</span></span></span><span lang="FA" style="background:white"><span style="line-height:92%"><span style="font-family:"B Zar"">) اثرات مفیدی در درمان <span style="letter-spacing:-.1pt">سپسیس دارند، اما هنوز اثرات تزریق همزمان </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="background:white"><span style="line-height:92%"><span style="letter-spacing:-.1pt">MSC</span></span></span></span><span lang="FA" style="background:white"><span style="line-height:92%"><span style="font-family:"B Zar""><span style="letter-spacing:-.1pt"> و </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="background:white"><span style="line-height:92%"><span style="letter-spacing:-.1pt">EPC</span></span></span></span><span style="background:white"><span style="line-height:92%"><span style="font-family:"B Zar""><span style="letter-spacing:-.1pt"> در درمان سپسیس بررسی نشده است. این مطالعه با هدف بررسی</span></span></span></span><span lang="FA" style="background:white"><span style="line-height:92%"><span style="font-family:"B Zar""> <span style="letter-spacing:-.1pt">اثر درمانی </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="background:white"><span style="line-height:92%"><span style="letter-spacing:-.1pt">EPC+MSC</span></span></span></span><span lang="FA" style="background:white"><span style="line-height:92%"><span style="font-family:"B Zar""><span style="letter-spacing:-.1pt"> بر کاهش پاسخ التهابی سیستمیک </span></span></span></span><span lang="FA" style="background:white"><span style="font-family:"B Zar""><span style="letter-spacing:-.1pt">در مدل سپسیس القایی ب</span></span></span><span lang="FA" style="background:white"><span style="line-height:92%"><span style="font-family:"B Zar""><span style="letter-spacing:-.1pt">ا لیپوپلی‏ساکارید(</span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="background:white"><span style="line-height:92%"><span style="letter-spacing:-.1pt">LPS</span></span></span></span><span lang="FA" style="background:white"><span style="line-height:92%"><span style="font-family:"B Zar""><span style="letter-spacing:-.1pt">)، انجام پذیرفت.</span></span></span></span><span lang="FA" style="line-height:92%"><span style="font-family:"B Zar""><span style="letter-spacing:-.1pt"></span></span></span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:92%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><span lang="AR-SA" style="line-height:92%"><span style="font-family:"B Koodak""><span style="letter-spacing:-.1pt">مواد و روش</span></span></span><span lang="FA" style="line-height:92%"><span style="font-family:"B Zar""><span style="letter-spacing:-.1pt"></span></span></span><span lang="AR-SA" style="line-height:92%"><span style="font-family:"B Koodak""><span style="letter-spacing:-.1pt">ها:</span></span></span> <span lang="FA" style="line-height:92%"><span style="font-family:"B Zar""><span style="letter-spacing:-.2pt">در این مطالعه تجربی،</span></span></span> <span lang="FA" style="line-height:92%"><span style="font-family:"B Zar""><span style="letter-spacing:-.2pt">سلول<span style="background:white">‏</span>های </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="letter-spacing:-.2pt">MSC</span></span></span><span lang="FA" style="line-height:92%"><span style="font-family:"B Zar""><span style="letter-spacing:-.2pt"> و</span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="letter-spacing:-.2pt">EPC</span></span></span><span lang="AR-SA" style="line-height:92%"><span style="font-family:"B Zar""><span style="letter-spacing:-.2pt"> مغز استخوان موش</span></span></span><span class="fontstyle01" style="font-family:"AdvOTee460ee4","serif""><span style="color:black"><span style="font-weight:normal"><span style="font-style:normal"><span lang="FA" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"B Zar""><span style="letter-spacing:-.2pt"> جداسازی و با استفاده از فلوسیتومتری، </span></span></span></span></span></span></span></span><span class="fontstyle01" style="font-family:"AdvOTee460ee4","serif""><span style="color:black"><span style="font-weight:normal"><span style="font-style:normal"><span lang="FA" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"B Zar""><span style="letter-spacing:-.1pt">نشانگرهای سطحی آن</span></span></span></span></span></span></span></span><span lang="AR-SA" style="letter-spacing:-.1pt"></span><span class="fontstyle01" style="font-family:"AdvOTee460ee4","serif""><span style="color:black"><span style="font-weight:normal"><span style="font-style:normal"><span lang="FA" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"B Zar""><span style="letter-spacing:-.1pt">ها بررسی شد. در مجموع 4 گروه موش (گروه‏های 12تایی) وارد مطالعه شدند. گروه کنترل که هیچ</span></span></span></span></span></span></span></span><span class="fontstyle01" style="font-family:"AdvOTee460ee4","serif""><span style="color:black"><span style="font-weight:normal"><span style="font-style:normal"><span lang="FA" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"B Zar""> تیماری نداشتند، گروه دوم </span></span></span></span></span></span></span><span class="fontstyle01" style="font-family:"AdvOTee460ee4","serif""><span style="color:black"><span style="font-weight:normal"><span style="font-style:normal"><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%">LPS</span></span></span></span></span></span> <span lang="FA" style="line-height:92%"><span style="font-family:"B Zar"">به صورت درون صفاقی، گروه سوم پس از تلقیح </span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%">LPS</span></span><span lang="FA" style="line-height:92%"><span style="font-family:"B Zar"">، بافر </span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%">PBS</span></span><span lang="FA" style="line-height:92%"><span style="font-family:"B Zar""> و گروه چهارم پس از <span style="letter-spacing:-.1pt">تلقیح </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="letter-spacing:-.1pt">LPS</span></span></span><span lang="FA" style="line-height:92%"><span style="font-family:"B Zar""><span style="letter-spacing:-.1pt">، سلول </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="letter-spacing:-.1pt">EPC</span></span></span><span lang="FA" style="line-height:92%"><span style="font-family:"B Zar""><span style="letter-spacing:-.1pt"> و </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="letter-spacing:-.1pt">MSC</span></span></span><span lang="FA" style="line-height:92%"><span style="font-family:"B Zar""><span style="letter-spacing:-.1pt"> دریافت کردند.</span></span></span> <span class="fontstyle01" style="font-family:"AdvOTee460ee4","serif""><span style="color:black"><span style="font-weight:normal"><span style="font-style:normal"><span lang="FA" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"B Zar""><span style="letter-spacing:-.1pt">سطح سرمی و بافتی </span></span></span></span></span></span></span></span><span class="fontstyle01" style="font-family:"AdvOTee460ee4","serif""><span style="color:black"><span style="font-weight:normal"><span style="font-style:normal"><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="letter-spacing:-.1pt">IL-1β</span></span></span></span></span></span></span><span class="fontstyle01" style="font-family:"AdvOTee460ee4","serif""><span style="color:black"><span style="font-weight:normal"><span style="font-style:normal"><span lang="FA" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"B Zar""><span style="letter-spacing:-.1pt">، </span></span></span></span></span></span></span></span><span class="fontstyle01" style="font-family:"AdvOTee460ee4","serif""><span style="color:black"><span style="font-weight:normal"><span style="font-style:normal"><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="letter-spacing:-.1pt">TNF-α</span></span></span></span></span></span></span><span class="fontstyle01" style="font-family:"AdvOTee460ee4","serif""><span style="color:black"><span style="font-weight:normal"><span style="font-style:normal"><span lang="FA" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"B Zar""><span style="letter-spacing:-.1pt">، </span></span></span></span></span></span></span></span><span class="fontstyle01" style="font-family:"AdvOTee460ee4","serif""><span style="color:black"><span style="font-weight:normal"><span style="font-style:normal"><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="letter-spacing:-.1pt">IL-6</span></span></span></span></span></span></span><span class="fontstyle01" style="font-family:"AdvOTee460ee4","serif""><span style="color:black"><span style="font-weight:normal"><span style="font-style:normal"><span lang="FA" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"B Zar""><span style="letter-spacing:-.1pt"> و </span></span></span></span></span></span></span></span><span class="fontstyle01" style="font-family:"AdvOTee460ee4","serif""><span style="color:black"><span style="font-weight:normal"><span style="font-style:normal"><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="letter-spacing:-.1pt">IL-10</span></span></span></span></span></span></span><span class="fontstyle01" style="font-family:"AdvOTee460ee4","serif""><span style="color:black"><span style="font-weight:normal"><span style="font-style:normal"><span lang="FA" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"B Zar""><span style="letter-spacing:-.1pt"> و سطوح سرمی </span></span></span></span></span></span></span></span><span class="fontstyle01" style="font-family:"AdvOTee460ee4","serif""><span style="color:black"><span style="font-weight:normal"><span style="font-style:normal"><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="letter-spacing:-.1pt">CRP</span></span></span></span></span></span></span><span class="fontstyle01" style="font-family:"AdvOTee460ee4","serif""><span style="color:black"><span style="font-weight:normal"><span style="font-style:normal"><span lang="FA" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"B Zar""> و آنزیم</span></span></span></span></span></span></span><span lang="FA" style="background:white"><span style="line-height:92%"><span style="font-family:"B Zar"">‏</span></span></span><span class="fontstyle01" style="font-family:"AdvOTee460ee4","serif""><span style="color:black"><span style="font-weight:normal"><span style="font-style:normal"><span lang="FA" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"B Zar"">‏های کبدی توسط کیت‏ تعیین شد.</span></span></span></span></span></span></span> <span lang="FA" style="line-height:92%"><span style="font-family:"B Zar"">از آنالیز واریانس یک طرفه و </span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%">post hoc</span></span><span lang="FA" style="line-height:92%"><span style="font-family:"B Zar""> برای مقایسه گروه‏ها استفاده شد.</span></span></span></span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:92%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><span lang="AR-SA" style="line-height:92%"><span style="font-family:"B Koodak""><span style="letter-spacing:-.1pt">یافته</span></span></span><span lang="FA" style="line-height:92%"><span style="font-family:"B Zar""><span style="letter-spacing:-.1pt"></span></span></span><span lang="AR-SA" style="line-height:92%"><span style="font-family:"B Koodak""><span style="letter-spacing:-.1pt">ها:</span></span></span> <span lang="FA" style="background:white"><span style="line-height:92%"><span style="font-family:"B Zar""><span style="letter-spacing:-.1pt">شاخص</span></span></span></span><span lang="FA" style="background:white"><span style="line-height:92%"><span style="font-family:"B Zar"">‏<span style="letter-spacing:-.1pt">های سطحی سلول</span><span style="letter-spacing:-.1pt">های </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="background:white"><span style="line-height:92%"><span style="letter-spacing:-.1pt">MSC</span></span></span></span><span lang="FA" style="background:white"><span style="line-height:92%"><span style="font-family:"B Zar""><span style="letter-spacing:-.1pt"> و </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="background:white"><span style="line-height:92%"><span style="letter-spacing:-.1pt">EPC</span></span></span></span><span lang="FA" style="background:white"><span style="line-height:92%"><span style="font-family:"B Zar""><span style="letter-spacing:-.1pt"> توسط</span></span></span></span><span class="fontstyle01" style="font-family:"AdvOTee460ee4","serif""><span style="color:black"><span style="font-weight:normal"><span style="font-style:normal"><span lang="FA" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"B Zar""><span style="letter-spacing:-.1pt"> فلوسایتومتری تایید شد. تزریق </span></span></span></span></span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="background:white"><span style="line-height:92%"><span style="letter-spacing:-.1pt">EPC+MSC</span></span></span></span><span style="background:white"><span style="line-height:92%"><span style="font-family:"B Zar""><span style="letter-spacing:-.1pt"> سطح سیتوکین</span></span></span></span><span lang="FA" style="background:white"><span style="line-height:92%"><span style="font-family:"B Zar""><span style="letter-spacing:-.1pt">‏های پیش التهابی را کاهش (0/001</span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="background:white"><span style="line-height:92%"><span style="letter-spacing:-.1pt">P<</span></span></span></span><span lang="FA" style="background:white"><span style="line-height:92%"><span style="font-family:"B Zar""><span style="letter-spacing:-.1pt">)، غلظت سیتوکین‏ ضد التهابی را افزایش (0/001</span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="background:white"><span style="line-height:92%"><span style="letter-spacing:-.1pt">P<</span></span></span></span><span lang="FA" style="background:white"><span style="line-height:92%"><span style="font-family:"B Zar""><span style="letter-spacing:-.1pt">) و میزان بقای موش‏های ناشی از </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="background:white"><span style="line-height:92%"><span style="letter-spacing:-.1pt">LPS</span></span></span></span><span lang="FA" style="background:white"><span style="line-height:92%"><span style="font-family:"B Zar""><span style="letter-spacing:-.1pt"> را افزایش داد (0/01</span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="background:white"><span style="line-height:92%"><span style="letter-spacing:-.1pt">P<</span></span></span></span><span lang="FA" style="background:white"><span style="line-height:92%"><span style="font-family:"B Zar""><span style="letter-spacing:-.1pt">). موش‏های تحت درمان با </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="background:white"><span style="line-height:92%"><span style="letter-spacing:-.1pt">EPC+MSC</span></span></span></span><span lang="FA" style="background:white"><span style="line-height:92%"><span style="font-family:"B Zar""><span style="letter-spacing:-.1pt">، کاهش قابل توجهی در آنزیم‏های کبدی (0/05</span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="background:white"><span style="line-height:92%"><span style="letter-spacing:-.1pt">P<</span></span></span></span><span lang="FA" style="background:white"><span style="line-height:92%"><span style="font-family:"B Zar""><span style="letter-spacing:-.1pt">)، ادم ریوی (0/01</span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="background:white"><span style="line-height:92%"><span style="letter-spacing:-.1pt">P<</span></span></span></span><span lang="FA" style="background:white"><span style="line-height:92%"><span style="font-family:"B Zar""><span style="letter-spacing:-.1pt">) و سطح </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="background:white"><span style="line-height:92%"><span style="letter-spacing:-.1pt">CRP</span></span></span></span><span style="background:white"><span style="line-height:92%"><span style="font-family:"B Zar""><span style="letter-spacing:-.1pt"> (0/05</span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="background:white"><span style="line-height:92%"><span style="letter-spacing:-.1pt">P<</span></span></span></span><span lang="FA" style="background:white"><span style="line-height:92%"><span style="font-family:"B Zar""><span style="letter-spacing:-.1pt">) در مقایسه با گروه </span></span></span></span><span lang="FA" style="background:white"><span style="line-height:92%"><span style="font-family:"B Zar"">سپسیس القایی <span style="letter-spacing:-.1pt">با </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="background:white"><span style="line-height:92%"><span style="letter-spacing:-.1pt">LPS</span></span></span></span><span lang="FA" style="background:white"><span style="line-height:92%"><span style="font-family:"B Zar""><span style="letter-spacing:-.1pt"> نشان دادند.</span></span></span></span><span lang="FA" style="line-height:92%"><span style="font-family:"B Zar""><span style="letter-spacing:-.1pt"></span></span></span></span></span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:92%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><span lang="AR-SA" style="line-height:92%"><span style="font-family:"B Koodak""><span style="letter-spacing:-.2pt">استنتاج:</span></span></span> <span class="fontstyle01" style="font-family:"AdvOTee460ee4","serif""><span style="color:black"><span style="font-weight:normal"><span style="font-style:normal"><span lang="FA" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"B Zar"">تزریق همزمان </span></span></span></span></span></span></span><span class="fontstyle01" style="font-family:"AdvOTee460ee4","serif""><span style="color:black"><span style="font-weight:normal"><span style="font-style:normal"><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%">EPC</span></span></span></span></span></span><span class="fontstyle01" style="font-family:"AdvOTee460ee4","serif""><span style="color:black"><span style="font-weight:normal"><span style="font-style:normal"><span lang="FA" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"B Zar""> و </span></span></span></span></span></span></span><span class="fontstyle01" style="font-family:"AdvOTee460ee4","serif""><span style="color:black"><span style="font-weight:normal"><span style="font-style:normal"><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%">MSC</span></span></span></span></span></span><span class="fontstyle01" style="font-family:"AdvOTee460ee4","serif""><span style="color:black"><span style="font-weight:normal"><span style="font-style:normal"><span lang="FA" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"B Zar""> منجر به کاهش پاسخ التهابی می‏</span></span></span></span></span></span></span><span lang="FA" style="background:white"><span style="line-height:92%"><span style="font-family:"B Zar"">‏</span></span></span><span class="fontstyle01" style="font-family:"AdvOTee460ee4","serif""><span style="color:black"><span style="font-weight:normal"><span style="font-style:normal"><span lang="FA" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"B Zar"">شود. </span></span></span></span></span></span></span><span lang="FA" style="background:white"><span style="line-height:92%"><span style="font-family:"B Zar"">این مطالعه نتایج امیدوارکننده‏ای برای درمان سپسیس ارائه میدهد</span></span></span><span lang="FA" style="line-height:92%"><span style="font-family:"B Zar"">.</span></span><span lang="AR-SA" style="line-height:92%"><span style="font-family:"B Zar""><span style="color:black"><span style="letter-spacing:-.2pt"></span></span></span></span></span></span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="line-height:135%"><span style="font-family:"Times New Roman","serif""><b><span style="font-size:10.0pt"><span style="line-height:135%"><span style="font-family:"Arial","sans-serif""><span style="letter-spacing:-.2pt">Background and purpose:</span></span></span></span></b> <span style="font-size:10.0pt"><span style="line-height:135%">Endothelial progenitor cells (EPCs) and mesenchymal stem cells (MSCs) have useful effects in treatment of sepsis, but the effects of co-injection of EPC and MSC for the treatment of sepsis have not yet been investigated.</span></span> <span style="font-size:10.0pt"><span style="line-height:135%">This study investigated the therapeutic effect of <span style="letter-spacing:-.2pt">MSC+EPC in reducing the </span></span></span><span style="font-size:9.5pt"><span style="line-height:135%"><span style="letter-spacing:-.2pt">systemic inflammatory response in lipopolysaccharide </span></span></span><span style="font-size:10.0pt"><span style="line-height:135%"><span style="letter-spacing:-.2pt">(LPS) induced sepsis in mice.</span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:135%"><span style="text-autospace:none"><span style="font-family:"Times New Roman","serif""><b><span style="font-size:10.0pt"><span style="line-height:135%"><span style="font-family:"Arial","sans-serif"">Materials and methods:</span></span></span></b><b> </b><span style="font-size:10.0pt"><span style="line-height:135%">In this experimental study, </span></span><span style="font-size:10.0pt"><span style="line-height:135%">mouse bone marrow MSCs and EPCs were isolated and their surface markers were investigated using flow cytometry. Four groups of mice (n= 12 per group) were included in the study.</span></span> <span style="font-size:10.0pt"><span style="line-height:135%">The control group had no treatment, group II received LPS intraperitoneally, group III received PBS buffer after LPS inoculation, and group IV received EPCs+MSCs after LPS inoculation. The serum and tissue levels of IL-1β, TNF-α, IL-6, and IL-10, and the serum levels of CRP and hepatic enzymes were determined by commercial kits.</span></span> <span style="font-size:10.0pt"><span style="line-height:135%">O</span></span><span lang="EN" style="font-size:10.0pt"><span style="line-height:135%">ne-way ANOVA and post hoc test were used for comparison between groups.</span></span></span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="line-height:135%"><span style="font-family:"Times New Roman","serif""><b><span style="font-size:10.0pt"><span style="line-height:135%"><span style="font-family:"Arial","sans-serif""><span style="letter-spacing:-.2pt">Results:</span></span></span></span></b><b> </b><span style="font-size:10.0pt"><span style="line-height:135%"><span style="letter-spacing:-.2pt">The surface markers of MSCs and EPCs were confirmed by flow cytometry. The co-injection of MSC+EPC significantly decreased the levels of pro-inflammatory cytokines (</span></span></span><span style="font-size:10.0pt"><span style="background:white"><span style="line-height:135%"><span style="letter-spacing:-.2pt">P<0.001)</span></span></span></span><span style="font-size:10.0pt"><span style="line-height:135%"><span style="letter-spacing:-.2pt">, increased the concentration of anti-inflammatory cytokines (</span></span></span><span style="font-size:10.0pt"><span style="background:white"><span style="line-height:135%"><span style="letter-spacing:-.2pt">P<0.001), and </span></span></span></span><span style="font-size:10.0pt"><span style="line-height:135%"><span style="letter-spacing:-.2pt">increased the survival rate (</span></span></span><span style="font-size:10.0pt"><span style="background:white"><span style="line-height:135%"><span style="letter-spacing:-.2pt">P<0.01)</span></span></span></span><span style="font-size:10.0pt"><span style="line-height:135%"><span style="letter-spacing:-.2pt">. Mice treated with MSC+EPC represented a significant decrease in liver enzymes (</span></span></span><span style="font-size:10.0pt"><span style="background:white"><span style="line-height:135%"><span style="letter-spacing:-.2pt">P<0.05)</span></span></span></span><span style="font-size:10.0pt"><span style="line-height:135%"><span style="letter-spacing:-.2pt">, pulmonary edema (</span></span></span><span style="font-size:10.0pt"><span style="background:white"><span style="line-height:135%"><span style="letter-spacing:-.2pt">P<0.01)</span></span></span></span><span style="font-size:10.0pt"><span style="line-height:135%"><span style="letter-spacing:-.2pt">, and CRP level (</span></span></span><span style="font-size:10.0pt"><span style="background:white"><span style="line-height:135%"><span style="letter-spacing:-.2pt">P<0.05) </span></span></span></span><span style="font-size:10.0pt"><span style="line-height:135%"><span style="letter-spacing:-.2pt">compared to the mice with LPS-induced sepsis.</span></span></span><span lang="EN" style="font-size:10.0pt"><span style="line-height:135%"><span style="letter-spacing:-.2pt"></span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="line-height:135%"><span style="vertical-align:top"><span style="font-family:"Times New Roman","serif""><b><span style="font-size:10.0pt"><span style="line-height:135%"><span style="font-family:"Arial","sans-serif"">Conclusion:</span></span></span></b> <span style="font-size:10.0pt"><span style="line-height:135%">Co-injection of MSC and EPC leads to a reduction of the inflammatory response. This study provides promising results for the </span></span><span lang="EN" style="font-size:10.0pt"><span style="line-height:135%">treatment of sepsis.</span></span><span dir="RTL" lang="AR-SA" style="line-height:135%"><span style="font-family:"B Zar""><span style="letter-spacing:-.2pt"></span></span></span></span></span></span></span><br>
لیپوپلی ساکارید, سپسیس, سلولهای پیشساز اندوتلیال, سلولهای بنیادی مزانشیمی
lipopolysaccharide, sepsis, endothelial progenitor cells, mesenchymal stem cells
1
14
http://jmums.mazums.ac.ir/browse.php?a_code=A-10-8089-5&slc_lang=fa&sid=1
Sadaf
Hoseinnia
صدف
حسین نیا
sadafhoseinnia@gmail.com
1003194753284600155769
1003194753284600155769
No
PhD Student in Microbiology, Department of Microbiology, North Tehran Branch, Islamic Azad University, Tehran, Iran
دانشجوی دکتری میکروبیولوژی، گروه میکروبیولوژی، واحد تهران شمال، دانشگاه آزاد اسلامی، تهران، ایران
Maryam
Ghane
مریم
قانع
ghane@iiau.ac.ir
1003194753284600155770
1003194753284600155770
Yes
Assistant Professor, Department of Microbiology, North Tehran Branch, Islamic Azad University, Tehran, Iran . Assistant Professor, Department of Biology, Islamshahr Branch, Islamic Azad University, Islamshahr, Iran
استادیار، گروه میکروبیولوژی، واحد تهران شمال، دانشگاه آزاد اسلامی، تهران، ایران . استادیار، گروه زیست شناسی واحد اسلامشهر، دانشگاه آزاد اسلامی، اسلامشهر، ایران
Jamile
Norouzi
جمیله
نوروزی
J_norouzi@iau-tnb.ac.ir
1003194753284600155771
1003194753284600155771
No
Professor, Department of Microbiology, North Tehran Branch, Islamic Azad University, Tehran, Iran
استاد، گروه میکروبیولوژی، واحد تهران شمال، دانشگاه آزاد اسلامی، تهران، ایران
Farzaneh
Hosseini
فرزانه
حسینی
hosseinimicrobiology@gmail.com
1003194753284600155772
1003194753284600155772
No
Assistant Professor, Department of Microbiology, North Tehran Branch, Islamic Azad University, Tehran, Iran
استادیار، گروه میکروبیولوژی، واحد تهران شمال، دانشگاه آزاد اسلامی، تهران، ایران