fa اثر هشت هفته تمرین شنا و مکمل‌ ویتامین E بر بیان ژن‌هایFKβ1 و P65 در موش‌های مدل آندومتریوز فيزيولوژي physiology گزارش کوتاه Brief Report <span style="font-size:11pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="text-autospace:none"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="line-height:115%"><span style="font-family:Calibri,sans-serif"><b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" koodak="" style="font-family:">&nbsp;</span></span></span></b></span></span></span></span></span></span></span></span><span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:90%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span lang="FA" style="line-height:90%"><span b="" koodak="" style="font-family:"><span style="color:black"><span style="letter-spacing:-.1pt">سابقه</span></span></span></span><span lang="AR-SA" style="line-height:90%"><span b="" koodak="" style="font-family:"><span style="color:black"><span style="letter-spacing:-.1pt"> و هدف:</span></span></span></span> <span lang="AR-SA" style="line-height:90%"><span b="" style="font-family:" zar="">اختلال در سیستم ایمنی بدن، از عوامل مهم ابتلا به آندومتریوز است. نقش فعالیت بدنی و مکمل‌های آنتی‌اکسیدانی بر تنظیم بیان ژن‌های مرتبط با بهبود سیستم ایمنی بدن،کاهش التهاب و عفونت، توسط مطالعات پیشین نشان داده شده است</span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:90%">.</span></span><span style="line-height:90%"><span b="" style="font-family:" zar=""> پژوهش حاضر به بررسی اثر تمرین شنا و مکمل ویتامین </span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:90%">E</span></span><span style="line-height:90%"><span b="" style="font-family:" zar=""> بر بیان ژن‌های </span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:90%">NFK&beta;1</span></span><span lang="AR-SA" style="line-height:90%"><span b="" style="font-family:" zar=""> و </span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:90%">P65</span></span><span style="line-height:90%"><span b="" style="font-family:" zar=""> در موش‌های مدل آندومتریوز می‌پردازد.</span></span><span lang="FA" style="line-height:90%"><span b="" style="font-family:" zar=""><span style="color:#0e101a"></span></span></span></span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:90%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span lang="AR-SA" style="line-height:90%"><span b="" koodak="" style="font-family:"><span style="color:black"><span style="letter-spacing:-.1pt">مواد و روش</span></span></span></span><span lang="FA" style="line-height:90%"><span b="" style="font-family:" zar=""><span style="color:black"><span style="letter-spacing:-.1pt">‌</span></span></span></span><span lang="AR-SA" style="line-height:90%"><span b="" koodak="" style="font-family:"><span style="color:black"><span style="letter-spacing:-.1pt">ها:</span></span></span></span> <span lang="AR-SA" style="line-height:90%"><span b="" style="font-family:" zar="">در این مطالعه<b>، </b>تعداد30 سر موش صحرایی ماده نژاد ویستار به 6 گروه مساوی با نام<span style="letter-spacing:-.1pt">‌</span>های </span></span><span lang="FA" style="line-height:90%"><span b="" style="font-family:" zar="">سالم، </span></span><span lang="AR-SA" style="line-height:90%"><span b="" style="font-family:" zar="">آندومتریوز</span></span><span lang="FA" style="line-height:90%"><span b="" style="font-family:" zar="">، بدون تمرین- سالین-</span></span><span lang="AR-SA" style="line-height:90%"><span b="" style="font-family:" zar=""> آندومتریوز</span></span><span lang="FA" style="line-height:90%"><span b="" style="font-family:" zar="">، تمرین- سالین-</span></span><span lang="AR-SA" style="line-height:90%"><span b="" style="font-family:" zar="">آندومتریوز</span></span><span lang="FA" style="line-height:90%"><span b="" style="font-family:" zar="">، بدون تمرین- مکمل-</span></span><span lang="AR-SA" style="line-height:90%"><span b="" style="font-family:" zar="">آندومتریوز </span></span><span lang="FA" style="line-height:90%"><span b="" style="font-family:" zar="">و تمرین-مکمل-</span></span><span lang="AR-SA" style="line-height:90%"><span b="" style="font-family:" zar="">آندومتریوز </span></span><span lang="FA" style="line-height:90%"><span b="" style="font-family:" zar="">تقسیم شدند. مداخلات براساس نام گروه اعمال گردید. </span></span><span lang="AR-SA" style="line-height:90%"><span b="" style="font-family:" zar="">آندومتریوز: با استفاده از جراحی ایجاد شد؛ فعالیت بدنی: هشت هفته تمرین شنا، پنج جلسه در هفته و 30 دقیقه در هر جلسه؛ مکمل: گاواژ روزانه 200 میلی‌گرم ویتامین </span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:90%">E</span></span> <span lang="FA" style="line-height:90%"><span b="" style="font-family:" zar="">به ازای</span></span> <span lang="AR-SA" style="line-height:90%"><span b="" style="font-family:" zar="">هر کیلو وزن بدن به مدت هشت هفته. 24 ساعت پس از آخرین جلسه تمرینی حیوانات کشته شدند و بافت آندومتریوز برداشته شد. بیان نسبی ژن‌های </span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:90%">NFKB1</span></span><span lang="AR-SA" style="line-height:90%"><span b="" style="font-family:" zar=""> و </span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:90%">P65</span></span><span lang="AR-SA" style="line-height:90%"><span b="" style="font-family:" zar=""> با استفاده از روش </span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:90%">Real-Time PCR</span></span><span style="line-height:90%"><span b="" style="font-family:" zar=""> اندازه‌گیری شد. داده‌ها با آزمون‌های آنالیز ورایانس یک‌طرفه و دوطرفه </span></span><span lang="FA" style="line-height:90%"><span b="" style="font-family:" zar="">تحلیل</span></span><span lang="AR-SA" style="line-height:90%"><span b="" style="font-family:" zar=""> شدند.</span></span><span lang="FA" style="line-height:90%"><span b="" style="font-family:" zar=""><span style="color:black"></span></span></span></span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:90%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span lang="AR-SA" style="line-height:90%"><span b="" koodak="" style="font-family:"><span style="color:black"><span style="letter-spacing:-.2pt">یافته</span></span></span></span><span lang="FA" style="line-height:90%"><span b="" style="font-family:" zar=""><span style="color:black"><span style="letter-spacing:-.2pt">‌</span></span></span></span><span lang="AR-SA" style="line-height:90%"><span b="" koodak="" style="font-family:"><span style="color:black"><span style="letter-spacing:-.2pt">ها:</span></span></span></span> <span lang="AR-SA" style="line-height:90%"><span b="" style="font-family:" zar="">القای آندومتریوز در حیوانات باعث افزایش معنی‌دار بیان ژن‌ها شد(</span></span><span lang="FA" style="line-height:90%"><span b="" style="font-family:" zar="">0/05</span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:90%">P<</span></span><span lang="AR-SA" style="line-height:90%"><span b="" style="font-family:" zar="">). تمرین و ویتامین </span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:90%">E</span></span><span style="line-height:90%"><span b="" style="font-family:" zar=""> به‌طور مستقل باعث کاهش معنی‌دار بیان ژن‌ها در موش‌های مدل آندومتریوز شدند(</span></span><span lang="FA" style="line-height:90%"><span b="" style="font-family:" zar="">0/05</span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:90%">P<</span></span><span lang="AR-SA" style="line-height:90%"><span b="" style="font-family:" zar="">). اثر سینرژیکی در مورد اثر تمرین و ویتامین </span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:90%">E</span></span><span lang="FA" style="line-height:90%"><span b="" style="font-family:" zar=""> بر متغیرهای وابسته پژوهش حاضر مشاهده نشد</span></span><span lang="AR-SA" style="line-height:90%"><span b="" style="font-family:" zar="">(</span></span><span lang="FA" style="line-height:90%"><span b="" style="font-family:" zar="">0/05</span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:90%">P></span></span><span lang="AR-SA" style="line-height:90%"><span b="" style="font-family:" zar="">)</span></span><span lang="FA" style="line-height:90%"><span b="" style="font-family:" zar="">.<span style="color:black"></span></span></span></span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:90%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span lang="AR-SA" style="line-height:90%"><span b="" koodak="" style="font-family:"><span style="color:black"><span style="letter-spacing:-.2pt">استنتاج:</span></span></span></span> <span lang="AR-SA" style="line-height:90%"><span b="" style="font-family:" zar="">براساس پژوهش حاضر، استفاده از تمرین شنا و مکمل ویتامین </span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:90%">E</span></span><span lang="FA" style="line-height:90%"><span b="" style="font-family:" zar=""> ممکن است به‌عنوان راهی در جهت بهبود آندومتریوز مطرح باشد.</span></span><span lang="AR-SA" style="line-height:90%"><span b="" style="font-family:" zar=""><span style="color:black"><span style="letter-spacing:-.2pt"></span></span></span></span></span></span></span></span></span></span></span><br> &nbsp; <span style="font-size:11pt"><span style="line-height:150%"><span style="font-family:Calibri,sans-serif"><b><span style="font-size:10.0pt"><span style="line-height:150%"><span arial="" style="font-family:">&nbsp;</span></span></span></b></span></span></span><span style="font-size:12pt"><span style="line-height:140%"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:10.0pt"><span style="line-height:140%"><span arial="" style="font-family:"><span style="letter-spacing:-.2pt">Background and purpose:</span></span></span></span></b> <span style="font-size:10.0pt"><span style="line-height:140%">Disturbance in the immune system is one of the main causes of endometriosis. Previous studies have shown the role of physical activity and antioxidant supplements on regulating the expression of genes related to improving the immune system, and reducing inflammation and infection. The present study examines the effect of swimming and vitamin E supplementation on the expression of NFK&beta;1 and P65 genes in endometriosis model rats.</span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:140%"><span style="text-autospace:none"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:10.0pt"><span style="line-height:140%"><span arial="" style="font-family:">Materials and methods:</span></span></span></b><b> </b><span style="font-size:10.0pt"><span style="line-height:140%">Thirty female rats were divided into six groups (n=5 per group); <span style="letter-spacing:-.2pt">healthy, endometriosis, without exercise-saline-endometriosis, exercise-saline-endometriosis, without exercise</span> <span style="letter-spacing:-.1pt">supplement-endometriosis, and exercise-supplement-endometriosis. Endometriosis was surgically induced.</span> Physical activity included swimming for eight weeks, five sessions/week and 30 minutes/session, and supplement was provided by daily gavage of 200 mg of vitamin E/kg for eight weeks. Twenty four hours after the last swimming session, the animals were sacrificed and the endometriosis tissue was removed. Relative expression levels of NFKB1 and P65 genes were measured using Real-Time PCR. Data were analyzed using one-way and two-way ANOVA.</span></span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="line-height:140%"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:10.0pt"><span style="line-height:140%"><span arial="" style="font-family:">Results</span></span></span></b><b><span style="font-size:10.0pt"><span style="line-height:140%">:</span></span></b><b> </b><span style="font-size:10.0pt"><span style="line-height:140%">Induction of endometriosis caused a significant increase in the expression of genes studied (P<0.05). Exercise and vitamin E independently caused a significant decrease in the genes expression in rats (P<0.05). No synergistic effect was observed regarding the effect of exercise and vitamin E on the dependent variables (P>0.05).</span></span></span></span></span><br> <span style="font-size:12pt"><span style="line-height:140%"><span style="vertical-align:top"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:10.0pt"><span style="line-height:140%"><span arial="" style="font-family:">Conclusion</span></span></span></b><b><span style="font-size:10.0pt"><span style="line-height:140%">:</span></span></b> <span style="font-size:10.0pt"><span style="line-height:140%">According to this study, swimming and vitamin E supplement can be considered in improving endometriosis.</span></span><i><span style="font-size:10.0pt"><span style="line-height:140%"></span></span></i></span></span></span></span><br> &nbsp; آندومتریوز, تمرین شنا, ویتامین E, NFKβ1, P65 endometriosis, swimming training, vitamin E, NFKβ1, P65 125 131 http://jmums.mazums.ac.ir/browse.php?a_code=A-10-16216-1&slc_lang=fa&sid=1 Soheila Heydarzadi سهیلا حیدرزادی soheilaheydarzadi942@gmail.com 1003194753284600156874 1003194753284600156874 No PhD Student, Department of Physical Education and Sports Sciences, North Tehran Branch, Islamic Azad University, Tehran, Iran دانشجوی دکتری، گروه تربیت‌بدنی و علوم ورزشی، واحد تهران شمال، دانشگاه آزاد اسلامی، تهران، ایران Lida Moradi لیدا مرادی moradi.lida@gmail.com 1003194753284600156875 1003194753284600156875 Yes Assistant Professor, Department of Physical Education and Sports Sciences, North Tehran Branch, Islamic Azad University, Tehran, Iran استادیار، گروه تربیت‌بدنی و علوم ورزشی، واحد تهران شمال، دانشگاه آزاد اسلامی، تهران، ایران Parvin Farzangi پروین فرزانگی parvin.farzanegi@gmail.com 1003194753284600156876 1003194753284600156876 No Associate Professor, Department of Sports Physiology, Sari Branch, Islamic Azad University, Sari, Iran دانشیار، گروه فیزیولوژی ورزشی، واحد ساری، دانشگاه آزاد اسلامی، ساری، ایران Bahram Abedi بهرام عابدی abedi_bahram@yahoo.com 1003194753284600156877 1003194753284600156877 No Professor, Department of Physical Education and Sports Sciences, North Tehran Branch, Islamic Azad University, Tehran, Iran استاد، گروه تربیت‌بدنی و علوم ورزشی، واحد تهران شمال، دانشگاه آزاد اسلامی، تهران، ایران