fa اثر مایع رویی کشت سلول‌های بنیادی مزانشیمی بر بیان ژن‌ کموکاین‌های CCL3 و CCL4 در نخاع رت‌های مبتلا به درد نوروپاتیک ايمونولوژي Immunology گزارش کوتاه Brief Report <span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:94%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span lang="FA" style="line-height:94%"><span b="" koodak="" style="font-family:"><span style="color:black"><span style="letter-spacing:-.1pt">سابقه</span></span></span></span><span lang="AR-SA" style="line-height:94%"><span b="" koodak="" style="font-family:"><span style="color:black"><span style="letter-spacing:-.1pt"> و هدف:</span></span></span></span> <span lang="FA" style="line-height:94%"><span b="" style="font-family:" zar="">التهاب، نقش مهمی در ایجاد درد نوروپاتیک دارد. نظر به اثرات ضدالتهابی سلول</span></span><span lang="AR-SA" style="font-size:11.5pt"><span style="line-height:94%"><span b="" style="font-family:" zar="">‌</span></span></span><span lang="FA" style="line-height:94%"><span b="" style="font-family:" zar="">های بنیادی مزانشیمی </span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:94%">(MSCs)</span></span><span lang="FA" style="line-height:94%"><span b="" style="font-family:" zar=""> و مایع</span></span><span lang="AR-SA" style="font-size:11.5pt"><span style="line-height:94%"><span b="" style="font-family:" zar="">‌</span></span></span><span lang="FA" style="line-height:94%"><span b="" style="font-family:" zar="">رویی کشت آن</span></span><span lang="AR-SA" style="font-size:11.5pt"><span style="line-height:94%"><span b="" style="font-family:" zar="">‌</span></span></span><span lang="FA" style="line-height:94%"><span b="" style="font-family:" zar="">ها </span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:94%">(MSCs-CM)</span></span><span lang="FA" style="line-height:94%"><span b="" style="font-family:" zar="">، هدف این مطالعه بررسی اثر </span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:94%">MSCs-CM</span></span><span lang="FA" style="line-height:94%"><span b="" style="font-family:" zar=""> بر میزان بیان ژن کموکاین</span></span><span lang="AR-SA" style="font-size:11.5pt"><span style="line-height:94%"><span b="" style="font-family:" zar="">‌</span></span></span><span lang="FA" style="line-height:94%"><span b="" style="font-family:" zar="">های </span></span><a name="_Hlk59642007"><span dir="LTR" style="font-size:10.0pt"><span style="line-height:94%">CCL3</span></span></a><span lang="FA" style="line-height:94%"><span b="" style="font-family:" zar=""> و </span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:94%">CCL4</span></span><span lang="FA" style="line-height:94%"><span b="" style="font-family:" zar=""> در نخاع رت</span></span><span lang="AR-SA" style="font-size:11.5pt"><span style="line-height:94%"><span b="" style="font-family:" zar="">‌</span></span></span><span lang="FA" style="line-height:94%"><span b="" style="font-family:" zar="">های نوروپاتیک بود.<span style="color:#0e101a"></span></span></span></span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:94%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span lang="AR-SA" style="line-height:94%"><span b="" koodak="" style="font-family:"><span style="color:black"><span style="letter-spacing:-.1pt">مواد و روش</span></span></span></span><span lang="FA" style="line-height:94%"><span b="" style="font-family:" zar=""><span style="color:black"><span style="letter-spacing:-.1pt">‌</span></span></span></span><span lang="AR-SA" style="line-height:94%"><span b="" koodak="" style="font-family:"><span style="color:black"><span style="letter-spacing:-.1pt">ها:</span></span></span></span> <span lang="FA" style="line-height:94%"><span b="" style="font-family:" zar="">در این مطالعه تجربی- مداخله</span></span><span lang="AR-SA" style="font-size:11.5pt"><span style="line-height:94%"><span b="" style="font-family:" zar="">‌</span></span></span><span lang="FA" style="line-height:94%"><span b="" style="font-family:" zar="">ای،</span></span> <span lang="FA" style="line-height:94%"><span b="" style="font-family:" zar="">28 رت نر نژاد ویستار با وزن 300-200 گرم وارد مطالعه شدند. درد نوروپاتی در رت</span></span><span lang="AR-SA" style="font-size:11.5pt"><span style="line-height:94%"><span b="" style="font-family:" zar="">‌</span></span></span><span lang="FA" style="line-height:94%"><span b="" style="font-family:" zar="">ها با عمل جراحی و تکنیک آسیب رساندن به عصب سیاتیک، از طریق گره زدن عصب مذکور با نخ</span></span><span lang="AR-SA" style="font-size:11.5pt"><span style="line-height:94%"><span b="" style="font-family:" zar="">‌</span></span></span><span lang="FA" style="line-height:94%"><span b="" style="font-family:" zar="">های مخصوص، القاء شد. سپس، </span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:94%">MSCs-CM</span></span><span lang="FA" style="line-height:94%"><span b="" style="font-family:" zar=""> به</span></span><span lang="AR-SA" style="font-size:11.5pt"><span style="line-height:94%"><span b="" style="font-family:" zar="">‌</span></span></span><span lang="FA" style="line-height:94%"><span b="" style="font-family:" zar="">صورت داخل صفاقی(یک م</span></span><span b="" lang="FA" style="font-family:" zar="">یلی</span><span b="" lang="AR-SA" style="font-family:" zar="">‌</span><span b="" lang="FA" style="font-family:" zar="">لیتر</span><span lang="FA" style="line-height:94%"><span b="" style="font-family:" zar="">) در سه روز متفاوت (روز قبل <span style="letter-spacing:-.1pt">جراحی (1-) و روزهای 7 و11 بعد از جراحی) به هر کدام از رت</span></span></span><span lang="AR-SA" style="font-size:11.5pt"><span style="line-height:94%"><span b="" style="font-family:" zar=""><span style="letter-spacing:-.1pt">‌</span></span></span></span><span lang="FA" style="line-height:94%"><span b="" style="font-family:" zar=""><span style="letter-spacing:-.1pt">های گروه آزمایش تزریق شد، درحالی</span></span></span><span lang="AR-SA" style="font-size:11.5pt"><span style="line-height:94%"><span b="" style="font-family:" zar=""><span style="letter-spacing:-.1pt">‌</span></span></span></span><span lang="FA" style="line-height:94%"><span b="" style="font-family:" zar=""><span style="letter-spacing:-.1pt">که رت</span></span></span><span lang="AR-SA" style="font-size:11.5pt"><span style="line-height:94%"><span b="" style="font-family:" zar=""><span style="letter-spacing:-.1pt">‌</span></span></span></span><span lang="FA" style="line-height:94%"><span b="" style="font-family:" zar=""><span style="letter-spacing:-.1pt">های گروه</span></span></span><span lang="FA" style="line-height:94%"><span b="" style="font-family:" zar=""> کنترل محیط کشت </span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:94%">DMEM</span></span><span lang="FA" style="line-height:94%"><span b="" style="font-family:" zar=""> را دریافت کردند. در پایان مطالعه (روز 15)، میزان بیان ژن</span></span><span lang="AR-SA" style="font-size:11.5pt"><span style="line-height:94%"><span b="" style="font-family:" zar="">‌</span></span></span><span lang="FA" style="line-height:94%"><span b="" style="font-family:" zar="">های </span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:94%">CCL3</span></span><span lang="FA" style="line-height:94%"><span b="" style="font-family:" zar=""> و </span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:94%">CCL4</span></span><span lang="FA" style="line-height:94%"><span b="" style="font-family:" zar=""> در نخاع حیوانات با </span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:94%">Real-time-PCR</span></span><span style="line-height:94%"><span b="" style="font-family:" zar=""> سنجش شد.<span style="color:black"></span></span></span></span></span></span></span></span></span></span><br> <span style="line-height: 94%;"><span style="direction: rtl;"><span style="unicode-bidi: embed;"><span lang="AR-SA" new="" roman="" style="font-family: " times=""><span b="" koodak="" style="font-family:"><span style="color:black"><span style="letter-spacing:-.2pt">یافته</span></span></span></span><span lang="AR-SA" new="" roman="" style="font-family: " times=""><span style="line-height:94%"><span b="" style="font-family:" zar="">‌</span></span></span><span lang="AR-SA" new="" roman="" style="font-family: " times=""><span b="" koodak="" style="font-family:"><span style="color:black"><span style="letter-spacing:-.2pt">ها: </span></span></span></span><span lang="FA" new="" roman="" style="font-family: " times=""><span b="" style="font-family:" zar=""><span style="letter-spacing:-.1pt">بررسی</span></span></span><span lang="AR-SA" new="" roman="" style="font-family: " times=""><span style="line-height:94%"><span b="" style="font-family:" zar="">‌</span></span></span><span lang="FA" new="" roman="" style="font-family: " times=""><span b="" style="font-family:" zar=""><span style="letter-spacing:-.1pt">های مولکولی</span></span></span><span lang="FA" new="" roman="" style="font-family: " times=""><span b="" style="font-family:" zar=""> کاهش معنی</span></span><span lang="AR-SA" new="" roman="" style="font-family: " times=""><span style="line-height:94%"><span b="" style="font-family:" zar="">‌</span></span></span><span lang="FA" new="" roman="" style="font-family: " times=""><span b="" style="font-family:" zar="">داری را در بیان</span></span><span lang="AR-SA" new="" roman="" style="font-family: " times=""><span style="line-height:94%"><span b="" style="font-family:" zar="">‌</span></span></span><span lang="FA" new="" roman="" style="font-family: " times=""><span b="" style="font-family:" zar="">ژن </span></span><span dir="LTR" new="" roman="" style="font-family: " times=""><span style="line-height:94%">CCL3</span></span><span new="" roman="" style="font-family: " times=""><span b="" style="font-family:" zar=""> درحیوانات دریافت</span></span><span lang="AR-SA" new="" roman="" style="font-family: " times=""><span style="line-height:94%"><span b="" style="font-family:" zar="">‌</span></span></span><span lang="FA" new="" roman="" style="font-family: " times=""><span b="" style="font-family:" zar="">کننده </span></span><span dir="LTR" new="" roman="" style="font-family: " times=""><span style="line-height:94%">MSCs-CM</span></span><span lang="FA" style="line-height: 94%;"><font face="B Zar"><span style="font-size: 12pt;"> نسبت به گروه کنترل نشان داد</span></font><span style="letter-spacing: -0.1pt;"><span b="" style="font-family: " zar="">(</span><font face="Times New Roman, serif"><span style="font-size: 13.3333px;">0/05>P</span></font></span></span><span lang="FA" new="" roman="" style="font-family: " times=""><span b="" style="font-family:" zar=""><span style="letter-spacing:-.1pt">)</span></span></span><span lang="FA" new="" roman="" style="font-family: " times=""><span b="" style="font-family:" zar="">، اما از نظر بیان ژن</span></span><span dir="LTR" new="" roman="" style="font-family: " times=""><span style="line-height:94%"> CCL4 </span></span><span lang="FA" new="" roman="" style="font-family: " times=""><span b="" style="font-family:" zar="">تفاوت معنی</span></span><span lang="AR-SA" new="" roman="" style="font-family: " times=""><span style="line-height:94%"><span b="" style="font-family:" zar="">‌</span></span></span><span lang="FA" new="" roman="" style="font-family: " times=""><span b="" style="font-family:" zar="">داری بین گروه</span></span><span lang="AR-SA" new="" roman="" style="font-family: " times=""><span style="line-height:94%"><span b="" style="font-family:" zar="">‌</span></span></span><span lang="FA" new="" roman="" style="font-family: " times=""><span b="" style="font-family:" zar="">ها مشاهده نشد. </span></span><span dir="LTR" new="" roman="" style="font-family: " times=""><span style="line-height:94%"><span style="color:black"></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:94%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span lang="AR-SA" style="line-height:94%"><span b="" koodak="" style="font-family:"><span style="color:black"><span style="letter-spacing:-.2pt">استنتاج:</span></span></span></span> <span lang="FA" style="line-height:94%"><span b="" style="font-family:" zar="">براساس یافته</span></span><span lang="AR-SA" style="font-size:11.5pt"><span style="line-height:94%"><span b="" style="font-family:" zar="">‌</span></span></span><span lang="FA" style="line-height:94%"><span b="" style="font-family:" zar="">های مطالعه حاضر، </span></span><span lang="FA" style="line-height:94%"><span b="" style="font-family:" zar=""><span style="letter-spacing:-.1pt">اثرات ضددردی </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:94%">MSCs-CM</span></span><span lang="FA" style="line-height:94%"><span b="" style="font-family:" zar=""><span style="letter-spacing:-.1pt">، تا حدی از طریق کاهش بیان کموکاین </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:94%"><span style="letter-spacing:-.1pt">CCL3</span></span></span><span lang="FA" style="line-height:94%"><span b="" style="font-family:" zar=""><span style="letter-spacing:-.1pt"> در نخاع میانجی</span></span></span><span lang="AR-SA" style="font-size:11.5pt"><span style="line-height:94%"><span b="" style="font-family:" zar="">‌</span></span></span><span lang="FA" style="line-height:94%"><span b="" style="font-family:" zar=""><span style="letter-spacing:-.1pt">گری می</span></span></span><span lang="AR-SA" style="font-size:11.5pt"><span style="line-height:94%"><span b="" style="font-family:" zar="">‌</span></span></span><span lang="FA" style="line-height:94%"><span b="" style="font-family:" zar=""><span style="letter-spacing:-.1pt">شود.</span></span></span></span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span dir="LTR" style="font-size:10.0pt"><span style="letter-spacing:-.1pt"></span></span></span></span></span></span></span></span> <span style="font-size:12pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:10.0pt"><span style="line-height:150%"><span arial="" style="font-family:"><span style="letter-spacing:-.2pt">Background and purpose:</span></span></span></span></b> <span style="font-size:10.0pt"><span style="line-height:150%">Inflammation plays an important role in the development of neuropathic pain (NP). </span></span><span lang="EN" style="font-size:10.0pt"><span style="line-height:150%">Considering the anti-inflammatory effects of mesenchymal stem cells (MSCs) and their </span></span><span style="font-size:10.0pt"><span style="line-height:150%">conditioned medium (CM), we aimed to investigate the effect of MSC-CM on the expression of CCL3 and CCL4 genes in the spinal cord of rats with NP.</span></span><span style="font-size:10.0pt"><span style="line-height:150%"></span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:150%"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:10.0pt"><span style="line-height:150%"><span arial="" style="font-family:">Materials and methods:</span></span></span></b><b> </b><span lang="EN" style="font-size:10.0pt"><span style="line-height:150%"><span style="letter-spacing:-.1pt">In this experimental-interventional study, 28 male Wistar rats weighing</span></span></span><span lang="EN" style="font-size:10.0pt"><span style="line-height:150%"> 200-300 grams were included.</span></span> <span style="font-size:10.0pt"><span style="line-height:150%">NP</span></span> <span style="font-size:10.0pt"><span style="line-height:150%">was induced in male rats using the chronic constriction injury (CCI)</span></span> <span style="font-size:10.0pt"><span style="line-height:150%">model of the sciatic nerve. MSCs-CM was injected intraperitoneally (1ml) into each rat in the experimental group one day before surgery (-1), and 7 and 11 days after the surgery, while the rats of control group received DMEM. At the end of the study (day 15), the expression level of CCL3 and CCL4 genes in the animals&rsquo; spinal cord was measured using Real time PCR.</span></span> <span style="font-size:9.0pt"><span style="line-height:150%"></span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:150%"><span style="text-autospace:none"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:10.0pt"><span style="line-height:150%"><span arial="" style="font-family:">Results: </span></span></span></b><span style="font-size:10.0pt"><span style="line-height:150%">Molecular studies showed a significant decrease in CCL3 gene expression (P<0.05) in CM treated animals compared to the control group, but in term of CCL4 gene expression, there was no significant difference between the groups.</span></span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:150%"><span style="text-autospace:none"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:10.0pt"><span style="line-height:150%"><span arial="" style="font-family:">Conclusion:</span></span></span></b> <span style="font-size:10.0pt"><span style="line-height:150%">Anti-NP effects of MSCs-CM seem to be partly mediated through downregulation of CCL3 chemokine in the spinal cord.</span></span><i><span style="font-size:10.0pt"><span style="line-height:150%"><span style="color:black"></span></span></span></i></span></span></span></span></span></span><br> &nbsp; درد نوروپاتیک, مایع‌رویی کشت سلول‌های بنیادی مزانشیمی, کموکاین, CCL3, CCL4, هایپرآلژزیا, آلودینیا neuropathic pain, mesenchymal stem cell, conditioned medium, CCL3, CCL4, hyperalgesia, allodynia 164 171 http://jmums.mazums.ac.ir/browse.php?a_code=A-10-16730-1&slc_lang=fa&sid=1 Mohammad-Shafi Mojadadi محمدشفیع مجددی mojadadi@gmail.com 1003194753284600161075 1003194753284600161075 No Associate Professor, Department of Immunology, School of Medicine, Sabzevar University of Medical Sciences, Sabzevar, Iran دانشیار، گروه ایمونولوژی، دانشکده پزشکی، دانشگاه علوم پزشکی سبزوار، سبزوار، ایران Kimia Shahrazad کیمیا شهرآزاد kimyashahrazad73@gmail.com 1003194753284600161076 1003194753284600161076 No Medical Student, Student Research Committee, Sabzevar University of Medical Sciences, Sabzevar, Iran دانشجوی پزشکی، کمیته تحقیقات دانشجویی، دانشگاه علوم پزشکی سبزوار، سبزوار، ایران Samad Nazemi صمد ناظمی samadnazemi@gmail.com 1003194753284600161077 1003194753284600161077 No Associate Professor, Cellular and Molecular Research Center, School of Medicine, Sabzevar University of Medical Sciences, Sabzevar, Iran دانشیار، مرکز تحقیقات سلولی و مولکولی، دانشکده پزشکی، دانشگاه علوم پزشکی سبزوار، سبزوار، ایران Saeideh Sadat Shobeiri سعیده سادات شبیری Saeideshobeiri@gmail.com 1003194753284600161078 1003194753284600161078 Yes PhD in Immunology, Cellular and Molecular Research Center, School of Medicine, Sabzevar University of Medical Sciences, Sabzevar, Iran دکتری تخصصی پژوهشی ایمونولوژی، مرکز تحقیقات سلولی و مولکولی، دانشکده پزشکی، دانشگاه علوم پزشکی سبزوار، سبزوار، ایران