Journal of Mazandaran University of Medical Sciences
مجله دانشگاه علوم پزشکی مازندران
J Mazandaran Univ Med Sci
Medical Sciences
http://jmums.mazums.ac.ir
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2017
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استخراج و شناسایی عصاره اندام های مختلف گیاه گزنه دو پایه Urtica dioica L و بررسی اثرات ضد باکتریایی و ضدقارچی آن
Extraction and Identification of Urtica dioica L Extract and Its Antibacterial and Antifungal Properties
ميکروبيولوژي
Microbiology
پژوهشي-کامل
Research(Original)
<span style="letter-spacing:-.1pt;"><span style="color:black;"><span style="font-family:b koodak;">سابقه</span></span></span><span style="letter-spacing:-.1pt;"><span style="color:black;"><span style="font-family:b koodak;"> و هدف:</span></span></span><span style="color:black;"><span style="font-family:b zar;"> گیاهان دارویی به</span></span><span style="font-family:b zar;"></span><span style="color:black;"><span style="font-family:b zar;">دلیل نداشتن عوارض داروهای شیمیایی، امروزه در جهان مورد توجه است. گیاه گزنه دو پایه </span></span><em><span dir="LTR"><span style="color:black;"><span style="font-size:10.0pt;">Urtica dioica</span></span></span></em><span dir="LTR"><span style="color:black;"><span style="font-size:10.0pt;"> L</span></span></span><span style="color:black;"><span style="font-family:b zar;"> دارای خاصیت درمانی در معالجه بیماری</span></span><span style="font-family:b zar;"></span><span style="color:black;"><span style="font-family:b zar;">های مزمن می</span></span><span style="letter-spacing:-.2pt;"><span style="color:black;"><span style="font-family:b zar;"></span></span></span><span style="color:black;"><span style="font-family:b zar;">باشد. این مطالعه به منظور تعیین خاصیت درمانی در شرایط برون</span></span><span style="letter-spacing:-.2pt;"><span style="color:black;"><span style="font-family:b zar;"></span></span></span><span style="color:black;"><span style="font-family:b zar;">تنی انجام شد.</span></span><br>
<span style="letter-spacing:-.1pt;"><span style="color:black;"><span style="font-family:b koodak;">مواد و روش</span></span></span><span style="letter-spacing:-.1pt;"><span style="color:black;"><span style="font-family:b zar;"></span></span></span><span style="letter-spacing:-.1pt;"><span style="color:black;"><span style="font-family:b koodak;">ها:</span></span></span><span style="color:black;"><span style="font-family:b zar;"> پس از جمع</span></span><span style="letter-spacing:-.2pt;"><span style="color:black;"><span style="font-family:b zar;"></span></span></span><span style="color:black;"><span style="font-family:b zar;">آوری قسمت</span></span><span style="font-family:b zar;"></span><span style="color:black;"><span style="font-family:b zar;">های مختلف گیاه گزنه به روش ماسیراسیون و پرکولاسیون، عصاره</span></span><span style="font-family:b zar;"></span><span style="color:black;"><span style="font-family:b zar;">ها تهیه شد. اثرات ضد باکتریایی این گیاه با استفاده از روش دیسک دیفیوژن (کربی-بائر) انجام و مقایسه گردید. تعیین </span></span><span dir="LTR"><span style="color:black;"><span style="font-size:10.0pt;">MIC</span></span></span><span style="color:black;"><span style="font-family:b zar;"> و </span></span><span dir="LTR"><span style="color:black;"><span style="font-size:10.0pt;">MBC</span></span></span><span style="color:black;"><span style="font-family:b zar;"> با استفاده از روش رقت لوله</span></span><span style="font-family:b zar;"></span><span style="color:black;"><span style="font-family:b zar;">ای (ماکرودایلوشن) با باکتری</span></span><span style="font-family:b zar;"></span><span style="color:black;"><span style="font-family:b zar;">های </span></span><span dir="LTR"><span style="color:black;"><span style="font-size:10.0pt;">(PTCC 1294)</span></span></span> <em><span dir="LTR"><span style="color:black;"><span style="font-size:10.0pt;">Listeria</span></span></span></em><span dir="LTR"><span style="color:black;"><span style="font-size:10.0pt;"> <em>Monocytogenes</em></span></span></span><span style="color:black;"><span style="font-family:b zar;"> و</span></span><em><span dir="LTR"><span style="color:black;"><span style="font-size:10.0pt;">Staphylococcus aureus</span></span></span></em><span dir="LTR"><span style="color:black;"><span style="font-size:10.0pt;"> (ATCC 25923)</span></span></span><span style="color:black;"><span style="font-family:b zar;"> و </span></span><em><span dir="LTR"><span style="color:black;"><span style="font-size:10.0pt;">Proteus vulgaris </span></span></span></em><span dir="LTR"><span style="color:black;"><span style="font-size:10.0pt;">(Atcc 13315)</span></span></span><span style="color:black;"><span style="font-family:b zar;"> و</span></span><em><span dir="LTR"><span style="color:black;"><span style="font-size:10.0pt;">Kellebsiella pneumoniae</span></span></span></em><span dir="LTR"><span style="color:black;"><span style="font-size:10.0pt;"> (PTCC 1053)</span></span></span><span style="color:black;"><span style="font-family:b zar;"> و مخمر </span></span><em><span dir="LTR"><span style="color:black;"><span style="font-size:10.0pt;">Candida albicans</span></span></span></em><span dir="LTR"><span style="color:black;"><span style="font-size:10.0pt;"> (ATCC 10231)</span></span></span><span style="color:black;"><span style="font-family:b zar;"> مورد بررسی قرار گرفت. شناسایی اجزای اصلی موجود در عصاره اندام</span></span><span style="letter-spacing:-.2pt;"><span style="color:black;"><span style="font-family:b zar;"></span></span></span><span style="color:black;"><span style="font-family:b zar;">های مختلف گیاه به وسیله دستگاه گاز کروماتوگراف </span></span><span dir="LTR"><span style="color:black;"><span style="font-size:10.0pt;">(GC)</span></span></span><span style="color:black;"><span style="font-family:b zar;"> انجام شد. ترکیب</span></span><span style="font-family:b zar;"></span><span style="color:black;"><span style="font-family:b zar;">های شناسایی شده در <span style="letter-spacing:-.2pt;">اسانس شامل 20 نوع بوده که مهم</span></span></span><span style="letter-spacing:-.2pt;"><span style="font-family:b zar;"></span></span><span style="letter-spacing:-.2pt;"><span style="color:black;"><span style="font-family:b zar;">ترین آن</span></span></span><span style="letter-spacing:-.2pt;"><span style="font-family:b zar;"></span></span><span style="letter-spacing:-.2pt;"><span style="color:black;"><span style="font-family:b zar;">ها شامل 5 ترکیب (21/25 درصد) </span></span></span><span dir="LTR" style="letter-spacing:-.2pt;"><span style="color:black;"><span style="font-size:10.0pt;">Neophytadiene </span></span></span><span style="letter-spacing:-.2pt;"><span style="color:black;"><span style="font-family:b zar;"> و (15/8 درصد)</span></span></span><span dir="LTR" style="letter-spacing:-.2pt;"><span style="color:black;"><span style="font-size:10.0pt;">Phtaleic acid </span></span></span><span style="letter-spacing:-.2pt;"><span style="color:black;"><span style="font-family:b zar;"> و (37/7 درصد)</span></span></span><span dir="LTR" style="letter-spacing:-.2pt;"><span style="color:black;"><span style="font-size:10.0pt;">phtaleate </span></span></span> <span dir="LTR" style="letter-spacing:-.2pt;"><span style="color:black;"><span style="font-size:10.0pt;">Dibutyl</span></span></span><span style="letter-spacing:-.2pt;"><span style="color:black;"><span style="font-family:b zar;"> و (32/6 درصد)</span></span></span><span dir="LTR" style="letter-spacing:-.2pt;"><span style="color:black;"><span style="font-size:10.0pt;">maleate </span></span></span> <span dir="LTR" style="letter-spacing:-.2pt;"><span style="color:black;"><span style="font-size:10.0pt;">Bis(2-ethyl hexyl)</span></span></span><span style="letter-spacing:-.2pt;"><span style="color:black;"><span style="font-family:b zar;"> و (62/7 درصد)</span></span></span><span dir="LTR" style="letter-spacing:-.2pt;"><span style="color:black;"><span style="font-size:10.0pt;">1,2 - benzenedi carboxylic acid </span></span></span><span style="letter-spacing:-.2pt;"><span style="color:black;"><span style="font-family:b zar;"> می</span></span></span><span style="letter-spacing:-.2pt;"><span style="font-family:b zar;"></span></span><span style="letter-spacing:-.2pt;"><span style="color:black;"><span style="font-family:b zar;">باشد که مقدار این ترکیبات در برگ نسبت به سایر اندام</span></span></span><span style="letter-spacing:-.2pt;"><span style="font-family:b zar;"></span></span><span style="letter-spacing:-.2pt;"><span style="color:black;"><span style="font-family:b zar;">ها دارای بیش</span></span></span><span style="letter-spacing:-.2pt;"><span style="font-family:b zar;"></span></span><span style="letter-spacing:-.2pt;"><span style="color:black;"><span style="font-family:b zar;">ترین مقدار بوده است</span></span></span><span style="color:black;"><span style="font-family:b zar;">.</span></span><br>
<span style="letter-spacing:-.2pt;"><span style="color:black;"><span style="font-family:b koodak;">یافته</span></span></span><span style="letter-spacing:-.2pt;"><span style="color:black;"><span style="font-family:b zar;"></span></span></span><span style="letter-spacing:-.2pt;"><span style="color:black;"><span style="font-family:b koodak;">ها:</span></span></span> <span style="letter-spacing:-.2pt;"><span style="color:black;"><span style="font-family:b zar;">عصاره</span></span></span><span style="letter-spacing:-.2pt;"><span style="color:black;"><span style="font-family:b zar;"></span></span></span><span style="letter-spacing:-.2pt;"><span style="color:black;"><span style="font-family:b zar;">های آبی بخش</span></span></span><span style="letter-spacing:-.2pt;"><span style="color:black;"><span style="font-family:b zar;"></span></span></span><span style="letter-spacing:-.2pt;"><span style="color:black;"><span style="font-family:b zar;">های مختلف گیاه نسبت به عصاره</span></span></span><span style="font-family:b zar;"></span><span style="letter-spacing:-.2pt;"><span style="color:black;"><span style="font-family:b zar;">های الکلی دارای بیش</span></span></span><span style="letter-spacing:-.2pt;"><span style="color:black;"><span style="font-family:b zar;"></span></span></span><span style="letter-spacing:-.2pt;"><span style="color:black;"><span style="font-family:b zar;">ترین اثر ضد باکتریایی بوده است. با توجه به این که عصاره آبی اندام</span></span></span><span style="font-family:b zar;"></span><span style="letter-spacing:-.2pt;"><span style="color:black;"><span style="font-family:b zar;">های برگ و ریشه در رقت </span></span></span><span dir="LTR" style="letter-spacing:-.2pt;"><span style="color:black;"><span style="font-size:10.0pt;">mg/ml</span></span></span><span style="letter-spacing:-.2pt;"><span style="color:black;"><span style="font-family:b zar;"> 5/0 بهترین اثر آنتی</span></span></span><span style="letter-spacing:-.2pt;"><span style="color:black;"><span style="font-family:b zar;"></span></span></span><span style="letter-spacing:-.2pt;"><span style="color:black;"><span style="font-family:b zar;">باکتریایی را داشته</span></span></span><span style="letter-spacing:-.2pt;"><span style="color:black;"><span style="font-family:b zar;"></span></span></span><span style="letter-spacing:-.2pt;"><span style="color:black;"><span style="font-family:b zar;">اند. عصاره آبی برگ گزنه در رقت</span></span></span><span style="letter-spacing:-.2pt;"><span style="color:black;"><span style="font-family:b zar;"></span></span></span><span style="letter-spacing:-.2pt;"><span style="color:black;"><span style="font-family:b zar;">های مختلف اثر ضد قارچی مناسب</span></span></span><span style="letter-spacing:-.2pt;"><span style="color:black;"><span style="font-family:b zar;"></span></span></span><span style="letter-spacing:-.2pt;"><span style="color:black;"><span style="font-family:b zar;">تری بر روی قارچ </span></span></span><em><span dir="LTR" style="letter-spacing:-.2pt;"><span style="color:black;"><span style="font-size:10.0pt;">Candida albicans</span></span></span></em><span style="letter-spacing:-.2pt;"><span style="color:black;"><span style="font-family:b zar;"> از خود نشان داد.</span></span></span><br>
<span style="letter-spacing:-.2pt;"><span style="color:black;"><span style="font-family:b koodak;">استنتاج:</span></span></span><span style="color:black;"><span style="font-family:b zar;"> با توجه به آزمایشات انجام شده، گیاه گزنه دو پایه </span></span><em><span dir="LTR"><span style="color:black;"><span style="font-size:10.0pt;">Urtica dioica</span></span></span></em><span style="color:black;"><span style="font-family:b zar;"> دارای خواص ضد باکتریایی و ضد قارچی می</span></span><span style="letter-spacing:-.2pt;"><span style="color:black;"><span style="font-family:b zar;"></span></span></span><span style="color:black;"><span style="font-family:b zar;">باشد و از این حیث می</span></span><span style="letter-spacing:-.2pt;"><span style="color:black;"><span style="font-family:b zar;"></span></span></span><span style="color:black;"><span style="font-family:b zar;">تواند علیه طیف وسیعی از میکروارگانیسم</span></span><span style="font-family:b zar;"></span><span style="color:black;"><span style="font-family:b zar;">ها اثر داشته باشد.</span></span><br>
<strong>Background and purpose:</strong> Medicinal plants have gained much interest in today’s world because they have fewer adverse effects compared to chemical drugs<span dir="RTL">.</span> <em>Urtica dioica L</em> could be used in treatment of some chronic diseases. This study aimed at investigating its treatment properties in vitro.<br>
<strong>Materials and methods:</strong> Different parts of the plant were collected and the extract was prepared using maceration and percolation. The antibacterial effects of the plant were identified using disk diffusion method. The MIC and MBC values were determined using dilution method against <em>Listeria Monocytogenes</em> (PTCC 1294), <em>Staphylococcus aureus</em> (ATCC 25923), <em>Proteus vulgaris</em> (ATCC 13315), <em>Kellebsiella pneumoniae</em> (PTCC 1053), and <em>Candida albicans</em> (ATCC 10231). The major components in the extract were identified by Gas Chromatography (GC). The extract consisted of 20 different combinations, including Neophytadiene (21.25%), Phthaleic acid (15.8%), phthalate Dibutyl (37.7%), maleate Bis (2-ethyl hexyl 32.6%), and 1,2 - Benzenedicarboxylic acid (62.7%). The amount of these compounds were higher in leaves.<br>
<strong>Results:</strong> The aqueous extract obtained from different organs of <em>Urtica dioica L</em> was found to have more antibacterial effect (the leaf and root at dilution of 5 mg /ml) compared to those of the alcoholic extract. In different dilutions, it also exhibited more antifungal effect on <em>Candida albicans</em>.<br>
<strong>Conclusion:</strong> According to this study, <em>Urtica dioica </em>has antibacterial and antifungal properties, therefore, it could be used against a broad spectrum of microorganisms.<br>
دیسک دیفیوژن, کروماتوگرافی گازی, گزنه دو پایه (Urtica dioica L), گیاهان دارویی, ماسیراسیون
disk diffusion, gas chromatography, Urtica dioica L, medicinal plants, maceration
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85
http://jmums.mazums.ac.ir/browse.php?a_code=A-10-5268-3&slc_lang=fa&sid=1
Pezhman
Moradi
پژمان
مرادی
100319475328460079446
100319475328460079446
No
Assistant Professor, Department of Horticultural Sciences, Faculty of Agriculture, Islamic Azad University, Saveh Branch, Saveh, Iran
استادیار، گروه علوم باغبانی، دانشکده کشاورزی، واحد ساوه، دانشگاه آزاد اسلامی، ساوه، ایران.
Kumarss
Amini
کیومرث
امینی
dr_kumarss_amini@yahoo.com
100319475328460079447
100319475328460079447
Yes
Assistant Professor, Department of Microbiology, Faculty of Basic Sciences, Islamic Azad University, Saveh Branch, Saveh, Iran
استادیار، گروه میکروبیولوژی، دانشکده علوم پایه، واحد ساوه، دانشگاه آزاد اسلامی، ساوه، ایران