Journal of Mazandaran University of Medical Sciences
مجله دانشگاه علوم پزشکی مازندران
J Mazandaran Univ Med Sci
Medical Sciences
http://jmums.mazums.ac.ir
1
admin
1735-9260
1735-9279
fa
jalali
1397
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gregorian
2018
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اثر شیلاجیت بر سطح سایتوکاین های پیش التهابی و
ضد التهابی در آسیب کبدی در موش صحرایی نر
Effect of Shilajit on the Levels of Pro-inflammatory and Anti-inflammation Cytokines in Hepatic Injury in Male Rats
فيزيولوژي
physiology
پژوهشي-کامل
Research(Original)
<p dir="RTL"><span style="letter-spacing:-.1pt;"><span style="color:black;"><span style="font-family:b koodak;">سابقه</span></span></span><span style="letter-spacing:-.1pt;"><span style="color:black;"><span style="font-family:b koodak;"> و هدف:</span></span></span> <span style="font-family:b zar;">سایتوکاین</span><span style="color:black;"><span style="font-family:b zar;"></span></span><span style="font-family:b zar;">ها</span> <span style="font-family:b zar;">طیف</span> <span style="font-family:b zar;">وسیعی</span> <span style="font-family:b zar;">از</span> <span style="font-family:b zar;">فرایندهای</span> <span style="font-family:b zar;">پاتولوژیکی</span> <span style="font-family:b zar;">و</span> <span style="font-family:b zar;">بیولوژیکی</span> <span style="font-family:b zar;">را</span> <span style="font-family:b zar;">تنظیم</span> <span style="font-family:b zar;">می</span><span style="color:black;"><span style="font-family:b zar;"></span></span><span style="font-family:b zar;">کنند</span> <span style="font-family:b zar;">و</span> <span style="font-family:b zar;">نقش</span> <span style="font-family:b zar;">مهمی</span> <span style="font-family:b zar;">در</span> <span style="font-family:b zar;">آسیب</span><span style="color:black;"><span style="font-family:b zar;"></span></span><span style="font-family:b zar;">های</span> <span style="font-family:b zar;">کبدی</span> <span style="font-family:b zar;">دارند. عوامل</span> <span style="font-family:b zar;">التهابی</span> <span style="font-family:b zar;">و</span> <span style="font-family:b zar;">اکسیدانی</span> <span style="font-family:b zar;">از</span> <span style="font-family:b zar;">علل</span> <span style="font-family:b zar;">مهم</span> <span style="font-family:b zar;">آسیب</span><span style="color:black;"><span style="font-family:b zar;"></span></span><span style="font-family:b zar;">های</span> <span style="font-family:b zar;">کبدی</span> <span style="font-family:b zar;">می</span><span style="color:black;"><span style="font-family:b zar;"></span></span><span style="font-family:b zar;">باشند.</span> <span style="font-family:b zar;">از آن</span><span style="color:black;"><span style="font-family:b zar;"></span></span><span style="font-family:b zar;">جایی</span><span style="color:black;"><span style="font-family:b zar;"></span></span><span style="font-family:b zar;">که</span> <span style="font-family:b zar;">شیلاجیت</span> <span style="font-family:b zar;">به</span> <span style="font-family:b zar;">عنوان</span> <span style="font-family:b zar;">یک</span> <span style="font-family:b zar;">عامل</span> <span style="font-family:b zar;">ضدالتهابی</span> <span style="font-family:b zar;">و</span> <span style="font-family:b zar;">آنتی</span><span style="color:black;"><span style="font-family:b zar;"></span></span><span style="font-family:b zar;">اکسیدانی</span> <span style="font-family:b zar;">شناخته</span> <span style="font-family:b zar;">می</span><span style="color:black;"><span style="font-family:b zar;"></span></span><span style="font-family:b zar;">شود، لذا</span> <span style="font-family:b zar;">هدف</span> <span style="font-family:b zar;">اصلی</span> <span style="font-family:b zar;">این</span> <span style="font-family:b zar;">مطالعه</span> <span style="font-family:b zar;">تعیین</span> <span style="font-family:b zar;">اثر</span> <span style="font-family:b zar;">شیلاجیت</span> <span style="font-family:b zar;">بر</span> <span style="font-family:b zar;">سطح</span> <span style="font-family:b zar;">سایتوکاین</span><span style="color:black;"><span style="font-family:b zar;"></span></span><span style="font-family:b zar;">های</span> <span style="font-family:b zar;">پیش</span><span style="color:black;"><span style="font-family:b zar;"></span></span><span style="font-family:b zar;">التهابی</span> <span style="font-family:b zar;">و</span> <span style="font-family:b zar;">ضد</span> <span style="font-family:b zar;">التهابی</span> <span style="font-family:b zar;">در</span> <span style="font-family:b zar;">آسیب</span> <span style="font-family:b zar;">کبد</span> <span style="font-family:b zar;">می</span><span style="color:black;"><span style="font-family:b zar;"></span></span><span style="font-family:b zar;">باشد.</span><span style="letter-spacing:-.1pt;"><span style="color:black;"><span style="font-family:b zar;"></span></span></span><br>
<span style="letter-spacing:-.1pt;"><span style="color:black;"><span style="font-family:b koodak;">مواد و روش</span></span></span><span style="letter-spacing:-.1pt;"><span style="color:black;"><span style="font-family:b zar;"></span></span></span><span style="letter-spacing:-.1pt;"><span style="color:black;"><span style="font-family:b koodak;">ها:</span></span></span> <span style="font-family:b zar;">در این مطالعه تجربی، 80 سر</span> <span style="font-family:b zar;">موش</span> <span style="font-family:b zar;">صحرایی</span> <span style="font-family:b zar;">نر</span> <span style="font-family:b zar;">نژاد</span> <span style="font-family:b zar;">ویستار</span> <span style="font-family:b zar;">به</span> <span style="font-family:b zar;">طور</span> <span style="font-family:b zar;">تصادفی</span> <span style="font-family:b zar;">به</span> <span style="font-family:b zar;">دو</span> <span style="font-family:b zar;">دسته </span><span dir="LTR"><span style="font-size:10.0pt;">A</span></span><span style="font-family:b zar;"> و </span><span dir="LTR"><span style="font-size:10.0pt;">B</span></span><span style="font-family:b zar;"> تقسیم</span> <span style="font-family:b zar;">شدند</span> <span style="font-family:b zar;">که</span> <span style="font-family:b zar;">هر</span> <span style="font-family:b zar;">دسته</span> <span style="font-family:b zar;">شامل</span> <span style="font-family:b zar;">5</span> <span style="font-family:b zar;">گروه کنترل،</span> <span style="font-family:b zar;">استامینوفن</span><span dir="LTR"><span style="font-size:10.0pt;">(A) </span></span><span style="font-family:b zar;"> و یا</span> <span style="font-family:b zar;">انسداد</span> <span style="font-family:b zar;">مجرای</span> <span style="font-family:b zar;">صفراوی </span><span dir="LTR"><span style="font-size:10.0pt;">(B)</span></span><span style="font-family:b zar;">،</span> <span style="font-family:b zar;">دوز</span> <span style="font-family:b zar;">کم</span> <span style="font-family:b zar;">مومیایی، دوز زیاد</span> <span style="font-family:b zar;">مومیایی</span> <span style="font-family:b zar;">و</span> <span style="font-family:b zar;">حلال</span> <span style="font-family:b zar;">می</span><span style="color:black;"><span style="font-family:b zar;"></span></span><span style="font-family:b zar;">باشند.</span> <span style="font-family:b zar;">تمامی</span> <span style="font-family:b zar;">گروه ها</span> <span style="font-family:b zar;">به جز</span> <span style="font-family:b zar;">گروه</span> <span style="font-family:b zar;">کنترل،</span> <span style="font-family:b zar;">در</span> <span style="font-family:b zar;">دسته</span><span dir="LTR"><span style="font-size:10.0pt;">A </span></span><span style="font-family:b zar;">،</span> <span style="font-family:b zar;">500</span> <span style="font-family:b zar;">میلی</span><span style="color:black;"><span style="font-family:b zar;"></span></span><span style="font-family:b zar;">گرم</span> <span style="font-family:b zar;">بر</span> <span style="font-family:b zar;">کیلوگرم</span> <span style="font-family:b zar;">استامینوفن</span> <span style="font-family:b zar;">به <span style="letter-spacing:-.2pt;">صورت</span></span> <span style="letter-spacing:-.2pt;"><span style="font-family:b zar;">داخل صفاقی</span></span> <span style="letter-spacing:-.2pt;"><span style="font-family:b zar;">دریافت</span></span> <span style="letter-spacing:-.2pt;"><span style="font-family:b zar;">کردند</span></span> <span style="letter-spacing:-.2pt;"><span style="font-family:b zar;">و</span></span> <span style="letter-spacing:-.2pt;"><span style="font-family:b zar;">در</span></span> <span style="letter-spacing:-.2pt;"><span style="font-family:b zar;">دسته </span></span><span dir="LTR" style="letter-spacing:-.2pt;"><span style="font-size:10.0pt;">B</span></span><span style="letter-spacing:-.2pt;"><span style="font-family:b zar;">،</span></span> <span style="letter-spacing:-.2pt;"><span style="font-family:b zar;">انسداد</span></span> <span style="letter-spacing:-.2pt;"><span style="font-family:b zar;">مجرای</span></span> <span style="letter-spacing:-.2pt;"><span style="font-family:b zar;">صفراوی</span></span> <span style="letter-spacing:-.2pt;"><span style="font-family:b zar;">انجام</span></span> <span style="letter-spacing:-.2pt;"><span style="font-family:b zar;">شد.</span></span> <span style="letter-spacing:-.2pt;"><span style="font-family:b zar;">سپس</span></span> <span style="letter-spacing:-.2pt;"><span style="font-family:b zar;">در</span></span> <span style="letter-spacing:-.2pt;"><span style="font-family:b zar;">دسته</span></span><span dir="LTR" style="letter-spacing:-.2pt;"><span style="font-size:10.0pt;">A </span></span><span style="letter-spacing:-.2pt;"><span style="font-family:b zar;"> پس</span></span> <span style="letter-spacing:-.2pt;"><span style="font-family:b zar;">از</span></span> <span style="letter-spacing:-.2pt;"><span style="font-family:b zar;">2</span></span> <span style="letter-spacing:-.2pt;"><span style="font-family:b zar;">ساعت و</span></span> <span style="letter-spacing:-.2pt;"><span style="font-family:b zar;">در دسته </span></span><span dir="LTR" style="letter-spacing:-.2pt;"><span style="font-size:10.0pt;">B</span></span><span style="letter-spacing:-.2pt;"><span style="font-family:b zar;">،</span></span> <span style="letter-spacing:-.2pt;"><span style="font-family:b zar;">هفت</span></span> <span style="letter-spacing:-.2pt;"><span style="font-family:b zar;">روز</span></span> <span style="letter-spacing:-.2pt;"><span style="font-family:b zar;">بعد</span></span> <span style="letter-spacing:-.2pt;"><span style="font-family:b zar;">از</span></span> <span style="letter-spacing:-.2pt;"><span style="font-family:b zar;">آسیب، به</span></span> <span style="letter-spacing:-.2pt;"><span style="font-family:b zar;">مدت</span></span> <span style="letter-spacing:-.2pt;"><span style="font-family:b zar;">یک</span></span> <span style="letter-spacing:-.2pt;"><span style="font-family:b zar;">و</span></span> <span style="letter-spacing:-.2pt;"><span style="font-family:b zar;">شش</span></span> <span style="letter-spacing:-.2pt;"><span style="font-family:b zar;">روز</span></span> <span style="letter-spacing:-.2pt;"><span style="font-family:b zar;">به ترتیب</span></span> <span style="letter-spacing:-.2pt;"><span style="font-family:b zar;">گروهای</span></span> <span style="letter-spacing:-.2pt;"><span style="font-family:b zar;">تحت</span></span> <span style="letter-spacing:-.2pt;"><span style="font-family:b zar;">درمان </span></span><span dir="LTR" style="letter-spacing:-.2pt;"><span style="font-size:10.0pt;">mg/kg</span></span><span style="letter-spacing:-.2pt;"><span style="font-family:b zar;"> 150 و</span></span><span dir="LTR" style="letter-spacing:-.2pt;"><span style="font-size:10.0pt;">mg/kg </span></span><span style="letter-spacing:-.2pt;"><span style="font-family:b zar;">250</span></span><span style="font-family:b zar;"> شیلاجیت</span> <span style="font-family:b zar;">و</span> <span style="font-family:b zar;">گروه</span> <span style="font-family:b zar;">حلال، آب</span> <span style="font-family:b zar;">مقطر</span> <span style="font-family:b zar;">به</span> <span style="font-family:b zar;">عنوان</span> <span style="font-family:b zar;">حلال</span> <span style="font-family:b zar;">شیلاجیت</span> <span style="font-family:b zar;">دریافت</span> <span style="font-family:b zar;">کردند. پس</span> <span style="font-family:b zar;">از</span> <span style="font-family:b zar;">اتمام</span> <span style="font-family:b zar;">دوره</span> <span style="font-family:b zar;">درمان،</span> <span style="font-family:b zar;">نمونه</span><span style="color:black;"><span style="font-family:b zar;"></span></span><span style="font-family:b zar;">ی</span> <span style="font-family:b zar;">کبد جهت انجام</span> <span style="font-family:b zar;">آزمایشات</span> <span style="font-family:b zar;">تهیه</span> <span style="font-family:b zar;">شد</span><span dir="LTR"><span style="font-size:10.0pt;">.</span></span><span style="color:black;"><span style="font-family:b zar;"></span></span><br>
<span style="letter-spacing:-.2pt;"><span style="color:black;"><span style="font-family:b koodak;">یافته</span></span></span><span style="letter-spacing:-.2pt;"><span style="color:black;"><span style="font-family:b zar;"></span></span></span><span style="letter-spacing:-.2pt;"><span style="color:black;"><span style="font-family:b koodak;">ها:</span></span></span> <span style="font-family:b zar;">درمان</span> <span style="font-family:b zar;">با</span> <span style="font-family:b zar;">دوز کم</span> <span style="font-family:b zar;">شیلاجیت، میزان</span> <span style="font-family:b zar;">سطح</span><span dir="LTR"><span style="font-size:10.0pt;">IL-10 </span></span><span style="font-family:b zar;">،</span><span dir="LTR"><span style="font-size:10.0pt;">IL-6 </span></span><span style="font-family:b zar;">،</span><span dir="LTR"><span style="font-size:10.0pt;"> IL-1β </span></span><span style="font-family:b zar;">و</span><span dir="LTR"><span style="font-size:10.0pt;">TNF- α </span></span><span style="font-family:b zar;"> که</span> <span style="font-family:b zar;">با</span> <span style="font-family:b zar;">مصرف</span> <span style="font-family:b zar;">تک</span> <span style="font-family:b zar;">دوز</span> <span style="font-family:b zar;">500</span> <span style="font-family:b zar;">میلی</span><span style="color:black;"><span style="font-family:b zar;"></span></span><span style="font-family:b zar;">گرم</span> <span style="font-family:b zar;">بر</span> <span style="font-family:b zar;">لیتر</span> <span style="font-family:b zar;">استامینوفن</span> <span style="font-family:b zar;">افزایش</span> <span style="font-family:b zar;">یافته</span> <span style="font-family:b zar;">بود،</span> <span style="font-family:b zar;">را</span> <span style="font-family:b zar;">کاهش</span> <span style="font-family:b zar;">داد</span><span style="font-family:b zar;"> (05/0</span><span dir="LTR"><span style="font-size:10.0pt;">p<</span></span><span style="font-family:b zar;">)</span><span style="font-family:b zar;">.</span><span style="color:black;"><span style="font-family:b zar;"></span></span><br>
<span style="letter-spacing:-.2pt;"><span style="color:black;"><span style="font-family:b koodak;">استنتاج:</span></span></span> <span style="font-family:b zar;">شیلاجیت</span> <span style="font-family:b zar;">در</span> <span style="font-family:b zar;">کاهش</span> <span style="font-family:b zar;">التهاب</span> <span style="font-family:b zar;">و</span> <span style="font-family:b zar;">آسیب</span><span style="color:black;"><span style="font-family:b zar;"></span></span><span style="font-family:b zar;">های</span> <span style="font-family:b zar;">کبدی</span> <span style="font-family:b zar;">ناشی</span> <span style="font-family:b zar;">از</span> <span style="font-family:b zar;">مصرف</span> <span style="font-family:b zar;">دوز</span> <span style="font-family:b zar;">زیاد</span> <span style="font-family:b zar;">استامینوفن</span> <span style="font-family:b zar;">موثر</span> <span style="font-family:b zar;">است.</span><span style="letter-spacing:-.2pt;"><span style="color:black;"><span style="font-family:b zar;"></span></span></span><br>
</p>
<p><strong>Background and purpose:</strong> Cytokines regulate a broad range of biological and pathological processes. They play an important role in liver damage. Inflammatory and antioxidant factors are important causes of liver damage. Shilajit is known as an anti-inflammatory and antioxidant agent. Therefore, the main aim of this study was to determine the effect of Shilajit on levels of proinflammatory and anti-inflammatory cytokines in liver injury.<br>
<strong>Materials and methods:</strong> In this experimental study, 80 male Wistar rats were randomly divided into two main groups: A and B. Each group was organized into five subgroups: control, acetaminophen (A) or bile duct obstruction (BDL) (B), low dose, high dose, and vehicle. All subgroups except the controls in group A received 500 mg/ kg acetaminophen via IP injection, while BDL was performed in all subgroups in group B. Then in group A, two hours and in group B seven days after injury, the treatment subgroups with drug and vehicle received 150 mg/kg (low dose) and 250 mg/kg (high dose)shilajit and distilled water as vehicle, for one and six days, respectively. After completing treatment, liver samples were taken for laboratory analysis.<br>
<strong>Results:</strong> Low-dose Shilajit treatment reduced IL-10, IL-6, IL-1β, and TNF- α levels, which had been increased by a single dose of 500 mg/kg acetaminophen (p<0.05).<span dir="RTL"></span><br>
<strong>Conclusion: </strong>Shilajit is effective in reducing inflammation and liver damage caused by high-dose consumption of acetaminophen.<br>
</p>
شیلاجیت, استامینوفن, مجرای صفراوی, سیتوکین, کبد
Shilajit, acetaminophen, bile duct, cytokine, liver
1
13
http://jmums.mazums.ac.ir/browse.php?a_code=A-10-8080-1&slc_lang=fa&sid=1
Farhood
Ghaaazi Firozsalari
فرهود
قاضی فیروزسالاری
100319475328460090473
100319475328460090473
No
دانشجوی کارشناسی ارشد فیزیولوژی، دانشگاه علوم پزشکی کرمان، کرمان، ایران
Nader
Shahrokhi
نادر
شاهرخی
nshahrokhisa@yahoo.com
100319475328460090474
100319475328460090474
Yes
دانشیار، مرکز تحقیقات فیزیولوژی، پژوهشکده علوم پایه و فیزیولوژی، دانشگاه علوم پزشکی کرمان، کرمان، ایران
Mohammad
Khaksari Hadad
محمد
خاکساری حداد
100319475328460090475
100319475328460090475
No
استاد، مرکز تحقیقات غدد و متابولیسم ، پژوهشکده علوم پایه و فیزیولوژی، دانشگاه علوم پزشکی کرمان ،کرمان، ایران
Gholamreza
Asadikaram
غلامرضا
اسدی کرم
100319475328460090476
100319475328460090476
No
استاد، مرکز تحقیقات غدد و متابولیسم ، پژوهشکده علوم پایه و فیزیولوژی، دانشگاه علوم پزشکی کرمان ،کرمان، ایران
Jaber
Atashbar
جابر
آتش بار
100319475328460090477
100319475328460090477
No
دانشجوی کارشناسی ارشد فیزیولوژی، دانشگاه علوم پزشکی کرمان، کرمان، ایران