Volume 15, Issue 48 (Oct 2005)
J Mazandaran Univ Med Sci 2005, 15(48): 1-10 |
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Eimani H, Hasani F, Haeri S, Esfahani M, Rezazadeh M, Dalman A, et al . Ect of Glutathion on resumpsion of Meiosi in-vitro Maturation, and development of immature Mouse oocytes. J Mazandaran Univ Med Sci 2005; 15 (48) :1-10
URL: http://jmums.mazums.ac.ir/article-1-1-en.html
URL: http://jmums.mazums.ac.ir/article-1-1-en.html
H Eimani 
, F Hasani , S.A Haeri , M.H Esfahani , M Rezazadeh , A Dalman , S KazemiAshtiani , A.H Shahverdi
, F Hasani , S.A Haeri , M.H Esfahani , M Rezazadeh , A Dalman , S KazemiAshtiani , A.H Shahverdi
Abstract: (10963 Views)
Background and purpose : In this study the effects of glutathion on resumption of meiosis, in-vitro maturation and development of immature mouse oocytes was evaluated.
Materials and methods : Immature oocytes recoverd from NMRI mouse strain (4-6 weeks) in four different experimental groups. Group 1 (control group): 345 immature oocytes were matured in MEM-α medium containing FCS 5%, group 1: 338 immature oocytes were cultured in MEM-α medium containing FCS 5% and 1mM glutathione, group 2: 237 immature oocytes curtured in MEM-α medium containing FCS 5%, 7.5 IuhCG, 100mIUrFSH and group 3: 327 immature oocytes were growed in MEM-α medium FCS5%, 7.5 IUhCG, 100mIUrFSH, 1mM glutathione. Fertilization and embryo development were done in T6 medium.
Results : There were significant differences between in-vitro maturation rates (group 1: 59.71%, group 2: 79.28%, group 3: 52.74 and group 4: 70.22%) and resumpsion of meiosis (group 1: 74.20%, group 2: 92.88%, group 3: 74.26%, group 4: 86.20%) in different groups.
Conclusion : The results of this study showed that glutathione (1mM) enhanced resumpsion of meiosis and in-vitro maturation and development of embryoes.
Materials and methods : Immature oocytes recoverd from NMRI mouse strain (4-6 weeks) in four different experimental groups. Group 1 (control group): 345 immature oocytes were matured in MEM-α medium containing FCS 5%, group 1: 338 immature oocytes were cultured in MEM-α medium containing FCS 5% and 1mM glutathione, group 2: 237 immature oocytes curtured in MEM-α medium containing FCS 5%, 7.5 IuhCG, 100mIUrFSH and group 3: 327 immature oocytes were growed in MEM-α medium FCS5%, 7.5 IUhCG, 100mIUrFSH, 1mM glutathione. Fertilization and embryo development were done in T6 medium.
Results : There were significant differences between in-vitro maturation rates (group 1: 59.71%, group 2: 79.28%, group 3: 52.74 and group 4: 70.22%) and resumpsion of meiosis (group 1: 74.20%, group 2: 92.88%, group 3: 74.26%, group 4: 86.20%) in different groups.
Conclusion : The results of this study showed that glutathione (1mM) enhanced resumpsion of meiosis and in-vitro maturation and development of embryoes.
Type of Study: Research(Original) |
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