Volume 21, Issue 84 (Sep 2011)                   J Mazandaran Univ Med Sci 2011, 21(84): 13-21 | Back to browse issues page

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Sadat S, Zabihollahi R, Javadi F, Vahabpour R, Siadat S, Azadmanesh K, et al . Immunological Evaluation of Recombinant HIV1 Virions with Novel Adjuvants in BALB/c Mice Model . J Mazandaran Univ Med Sci. 2011; 21 (84) :13-21
URL: http://jmums.mazums.ac.ir/article-1-665-en.html
Abstract:   (12059 Views)
Background and purpose: Finding an efficient vaccine against HÏV infection is still a major concern and a great challenge. Ïn our previous study, HÏV-1 virions with capability of a single replication were produced. Ïn the present study their immunological evaluation has been carried out to study their induction of cellular and humoral responses in mice model.
Materials and methods: Ïn this study, first and second virion generations were produced in human HËK293T cells, and were injected to three different mice groups in three doses with or without adjuvants. Âfter the last injection, immune responses were evaluated in serum and spleen cells of mice using ËLÏSÂ.
Results: Âlthough all animals vaccinated with different immunogen regimens did develop cellular responses, in the main group injected with novel adjuvants, the highest levels of specific antibody, ÏFN-γ, ÏL-4 an also most ÏFN-γ excreting cells were evident, which is suggestive of a strong cellular response in these cells.
Çonclusion: Ân effective vaccine against HÏV requires induction of strong humoral and cellular responses. Hence, these results demonstrate the high immune system stimulating capabilities of virion injection regime accompanied by M720 and ÇpG adjuvants, which provides the potential for an efficient HÏV vaccine.
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Type of Study: Research(Original) |

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