Showing 3 results for Asadi-Fard
Layasadat Khorsandi, Abbas Heidari-Moghadam, Darioush Bijan Nejad, Mahmoud Orazizadeh, Fereshteh Nejaddehbashi, Yousef Asadi-Fard,
Volume 33, Issue 1 (11-2023)
Abstract
Background and purpose: Type 1 diabetes (T1D) is an insulin-dependent disease, that results from the destruction of pancreatic islets beta cells. The main objective of this study was to determine the effects of decellularized gastric matrix (DSM) on the function of Min6 cells (a beta cell line).
Materials and methods: In this experimental study, the stomachs of Wistar rats were decellularized with sodium dodecyl sulfate (SDS) and Triton X-100. The DSM was characterized by scanning electron microscopy, determination of residual DNA and histological examinations. After seeding the Min6 cells on the DSM scaffold, the glucose challenge test and mRNA expression of insulin-related genes were examined to evaluate the function of the cells.
Results: The main components of the DSM, such as collagens and glycosaminoglycans, remained intact after decellularization. In addition, the very low DNA residues and the suitable mechanical behavior of the DSM provided an ideal extracellular microenvironment for the Min6 cells. The glucose challenge test showed that the seeded cells secreted more insulin and C-peptide in the DSM than in the 2D culture. The expression of specific insulin-related genes such as Pdx-1, INS, MAF-A, and Glut-2 was significantly higher in the recellularized scaffold than in the traditional 2D cells.
Conclusion: These results show that DSM is a suitable scaffold for the stabilization of artificial pancreatic islets.
Abbas Heidari-Moghadam, Yousef Asadi-Fard, Zeinab Najaf-Abadi, Hodasadat Sharif, Afrooz Moradkhani,
Volume 34, Issue 233 (5-2024)
Abstract
Background and purpose: Knee joint disorders are divided into two general categories of traumatic and non-traumatic injuries, including skeletal deformities such as genu valgum (knocked knee) and genu varum (bowed legs). Genu valgum and genu varum are two of the most common deformities of the knee joint. These deformities can directly affect the quality of people's social lives. Considering the importance of determining its rate in the youth community, this study aims to determine the relationship between genu valgum and genu varum with anthropometric indices in elementary school students of Dezful City in 2022.
Materials and methods: The current research was cross-sectional-analytical, which was conducted in 2022 at the level of elementary schools in Dezful City on 400 students of girls' and boys' schools between 7 and 11 years old. Patient demographic information, including age, gender, height, weight, family history, and hip joint width, was gathered using a questionnaire. To evaluate the deformation of the lower limb, the participants must stand in a way that the knee is in full extension and the ankles of both legs should be close together so that the patella bones on both sides are facing forward. In this position, deformation of the knee joint (genu valgum and genu varum) was determined by measuring the distances between the medial condyles of the femur and the medial malleolus of the ankle, and its relationship with demographic information was evaluated.
Results: The frequency distribution showed that 2.5% of the studied subjects had genu valgum, and 11.5% of them did not have genu varum. These results showed the existence of skeletal disorders in knee joints at the rate of 14% in the studied population. Also, the results of this study showed that only 2.2% of the studied population had a family history of musculoskeletal disorders such as genu valgum and genu varum, and 97.5% of them had no family history, which indicated the absence of a significant relationship between the occurrence of disorders and family history (P=0.187). Results showed that there was a significant relationship between the average age and the observation of genu varum and genu valgum disorder (P<0.001). Also, a significant relationship was observed between height and weight and genu varum and genu valgum (P=0.001). It is noteworthy that a significant relationship was observed between the width of the hip joint with these abnormalities (P<0.05).
Conclusion: Although the rate of disorders in the sample population was not very impressive, investigations in this study have confirmed a significant relationship between demographic indicators and knee joint disorders. The existence of a relationship between height, weight, age, and other important anatomical indicators, such as the distance between the hip joint and genu varum and genu valgum, probably indicates the increasing trend of observing these disorders in society with growth and changes in the skeletal framework, which requires further evaluation. The findings of this research can be utilized by health policy institutions to mitigate the prevalence of this condition, particularly among the younger population, through implementing suitable plans and strategies.
Nastaran Azarbarz, Yousef Asadi-Fard, Hana Sheykhi, Layasadat Khorsandi,
Volume 35, Issue 244 (5-2025)
Abstract
Background and purpose: Sodium arsenite (SA) is recognized as a common mineral contaminant in drinking water, significantly affecting the cardiovascular, neuroendocrine, urinary, and digestive systems. Naringenin (NG) is a flavonoid with antioxidant and anti-inflammatory properties that can prevent liver damage caused by various factors. This study was conducted to examine the protective role of NG against SA-induced hepatotoxicity in mice.
Materials and methods: In this experimental study, 28 mice were allocated into four groups: (I) Control, (II) NG (50 mg/kg), (III) SA (40 mg/L), and (IV) SA+NG. NG and SA were administered orally for five weeks. Histological changes, hepatic enzyme levels (ALT and AST), oxidative stress markers (SOD, CAT, and MDA), and inflammatory biomarkers (Nrf2 and NF-kB) were assessed using biochemical and histological methods. The data were then analyzed using one-way ANOVA and Tukey’s post hoc test in SPSS software.
Results: SA caused histological damage, characterized by a significant increase in the infiltration of inflammatory cells and the accumulation of red blood cells (P< 0.001). It also significantly elevated alanine transaminase (ALT) and aspartate aminotransferase (AST) levels (P<0.05). Moreover, SA significantly upregulated nuclear factor kappa B (NF-kB) expression and downregulated nuclear factor-erythroid-2 related factor 2 (Nrf2) expression. NG reduced ALT and AST activity levels, reversed histological alterations and antioxidant capacity. Furthermore, NG enhanced catalase activity levels, superoxide dismutase (SOD) activity, and Nrf2 expression.
Conclusion: SA induces hepatotoxicity by increasing oxidative stress and inflammatory responses. The administration of NG effectively mitigated the harmful effects of the imbalance of oxidative stress and antioxidant pathways and the histopathological changes observed in SA-intoxicated mice
