O Emadian, F Naghshvar, Zh Torabizade, A.r Yaseri, A.r Khalilian, M Shahrbandian,
Volume 21, Issue 82 (May 2011)
Abstract
Background and purpose: Gastric carcinoma is one of the most common malignancies and its early diagnosis can be effective in the treatment method and the rate of the patients’ survival. Ïn the past recent years, many genes have been identified which may have relationship with the prognosis of this disease and the patient’s survival. This study is going to investigate the rate and pattern of p53 and HËR2 expression in gastric carcinoma by immunohistochemistry (ÏHÇ).
Materials and methods: 114 paraffin wax-embedded gastric carcinoma specimens were selected and the results obtained from ÏHÇ stains were compared with histopathologic findings. The data were analyzed using Çhi Square test in SPSS.
Results: Ïn this study, male to female ratio was 1.92 with the average age of 65.44 ± 10.92. The most common tumor locations were antrum or pylorus (54 cases) and the average size of tumor was
5.32 ± 2.62 cm. Âccording to Lauren classification, intestinal tumor (101 cases) and in Ming classification, infiltrative tumor (74 cases) were the most prevailing. The most dominant cases were grade 2 (58 cases) and T3 staging (76 cases). Lymph nodes metastasis was also observed in 70 cases. The amount of p53 and HËR2 expression were 52.6% and 24.6% of gastric carcinomas, respectively, showing a significant correlation between these two (P<0.05). Âlso a significant correlation (P<0.05) was found between the rate of p53 expression and tumor size, location of tumor and lymph node metastasis (P=0.05) in gastric cancers. Besides, HËR2 protein had significant correlation with age of patient, tumor size, and Lauren’s classification intestinal type carcinoma (P<0.05).
Çonclusion: The results of the study indicated that the incidence of p53 protein is associated with larger tumor size and lymph node metastasis. HËR2 protein and its expression in gastric cancer are similar to ductal adenocarcinoma in breast cancer.
Saba Kalantary, Farideh Golbabaei, Masoud Latifi, Mohammad Ali Shokrgozar, Mehdi Yaseri,
Volume 30, Issue 186 (7-2020)
Abstract
Background and purpose: Some skin injuries are caused by occupational skin exposures that are resulted from production of reactive oxygen species and oxidative stress (ROS). Thus, protecting the skin is a key issue. Recently, several surveys have introduced novel alternatives such as nanofibers for skin protection. In this research, Poly (ε-caprolactone) (PCL)/gelatin (Gt)/ Vitamin E (VE) was investigated as a nanofiber antioxidant protective layer in occupational skin exposures.
Materials and methods: Vitamin E was combined with PCL/Gt nanofiber mats by electrospinning method. The diameter of nanofibers and chemical characteristics were determined using scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR). Adhesion characteristics and proliferation of human dermal fibroblast cells (HDFC) on nanofibers were analyzed by Methylthiazol Tetrazolium (MTT) assay. Finally, we studied the application of PCL/Gt/VE nanofibers against oxidation stress induced by tert-butyl hydroperoxide (t-BHP).
Results: With increase in Vitamin E levels, the PCL/Gt/VE nanofibers were found to be thiner. The culture of HDF cells on nanofibers showed that adding Vitamin E to PCL/Gt mats caused increased attachment and proliferation of the cells. PCL/Gt/VE nanofibers significantly assisted human skin cells against ROS (P< 0.001).
Conclusion: According to current study, PCL/Gt/VE nanofibers could be an appropriate candidate for protecting skin against ROS as a novel method to maintain workers’ health.
