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Showing 3 results for Spinal Cord Injury

Bagher Pourheydar, Alireza Pourali, Gholamhossein Farjah, Mojtaba Karimipour, Behnam Heshmatian, Maryam Pourheydar,
Volume 27, Issue 157 (2-2018)
Abstract

Background and purpose: Spinal cord injury (SCI) is one of the most disabling diseases with various physical and psychological consequences. One of the treatments of SCI is using agents that have neuroprotective effects such as Vitamin C and ubiquinone. This research aimed at investigating the effect of co-administration of these agents on rat experimental model of SCI.
Materials and methods: Adult male Wistar rats (n=40) were divided into sham, lesion, AA, CO10, and AA+COQ10 groups. In sham group only laminectomy was performed and in other groups contusion model of SCI was done. After 24 hr, the animals in AA, COQ10, and AA+COQ10 groups received intraperitoneal injection of AA, COQ10 and AA+COQ10, respectively. Then, behavioral assessment and biochemical study were performed. Eight weeks after treatment, the brains were removed and 5µ sections were prepared. Finally, cresyl violet staining was done.
Results: There was a significant difference in BBB score of AA, COQ10 groups compared to that of the AA+COQ10 group (P<0.001). A Significant difference was also seen in MDA level in AA+COQ10 group compared to that in lesion group (P=0.017). Compared with the lesion group, the serum GR level in AA+COQ10 group significantly increased (P=0.018). The average number of normal motor neurons in anterior spinal horn significantly increased in treatment groups compared to that in lesion group (P<0.001)
Conclusion: Co-administration of AA and COQ10 in contusion model of SCI led to functional recovery, reduction of MDA, increase of GR level in serum and increase of motor neurons survival in anterior spinal horn.
 


Eskandar Garmei, Ali Yaghoubi, Najmeh Rezaeian,
Volume 34, Issue 233 (5-2024)
Abstract

Background and purpose: Atrophy of the skeletal system caused by spinal cord injury (SCI) can cause secondary systemic metabolic dysfunction, such as glucose intolerance, type 2 diabetes, and insulin resistance. On the other hand, Sirtun1 (SIRT1) and Peroxisome Proliferator-Activated Receptor-Gamma Coactivator-1alpha (PGC-1α) are known factors and related proteins involved in skeletal muscle atrophy. Therefore, the present study aimed to investigate the effect of aerobic training with resveratrol consumption on SIRT1 and PGC-1α levels in the gastrocnemius muscles of rats after spinal cord injury.
Materials and methods: 36 male Wistar rats aged eight weeks were randomly placed in 4 groups including control, resveratrol, training, and resveratrol+training (each group n=9). After anesthesia, an incision was made in the midline of the back and over the vertebral ridges. The muscles and lamina of the T9 vertebra were removed without damaging the dura mater, Spinal cord injury was caused by dropping a ten-gram weight from a height of 25 mm on the spinal cord in the T10 segment. After confirmation of SCI, a Resveratrol supplement with a dose of 10 mg/kg was injected intraperitoneally and daily (every morning for 4 weeks), and the rats of other groups were injected with the same amount of saline. The aerobic training was carried out with the help of the weight support system for 4 weeks, 5 sessions per week, each session was 58 minutes and the intensity was 20 m/min. 48 hours after the last training session, the gastrocnemius muscle of right leg of all rats was removed. The SIRT1 and PGC-1α levels in the gastrocnemius muscle were measured by the ELISA method. To analyze the data, one-way analysis of variance and LSD post hoc tests were used at the significance level of P<0.05.
Results: SIRT1 levels in the gastrocnemius muscle of resveratrol and resveratrol+training groups were significantly higher than the control group (P-value respectively 0.023 and 0.007), but despite the increase in the level of this index in the training group compared to the control group, no significant difference was observed (P=0.399). SIRT1 levels in gastrocnemius muscle of resveratrol+training groups were significantly higher than training group (P=0.038). PGC-1α levels in gastrocnemius muscle of training and resveratrol+ training groups were significantly higher than control group (P-value respectively 0.024 and 0.007), but despite the increase in the level of this index in the resveratrol group compared to the control group, no significant difference was observed (P=0.449). PGC-1α levels in gastrocnemius muscle of resveratrol+training groups were significantly higher than reveratrol group (P=0.023).
Conclusion: These results show the positive effect of resveratrol consumption, on SIRT1 level, as well as aerobic exercise on PGC-1α level of the biceps muscle in rats with SCI. It has been pointed out that the increase in SIRT1 expression and the subsequent increase in PGC-1α leads to an increase in the number of myonuclei, which improves the recovery process after injury and plays an active role in muscle hypertrophy by upregulating and reducing catabolic processes. herefore, it appears that a combination of aerobic training and resveratrol supplementation can mitigate muscle atrophy caused by spinal cord injury in muscles below the level of injury by influencing the levels of muscle SIRT1 and PGC-1α

Mohsen Rezaei, Mohammad Taghi Mohammadi, Hassan Qoshooni, Farideh Bahrami, Zahra Bahari, Shima Shahyad, Raheleh Halabian,
Volume 35, Issue 243 (3-2025)
Abstract

Background and purpose: Spinal cord injuries are usually severe and currently lack effective treatment. Bone marrow stem cells (BMSCs) have significant potential for repair by reducing inflammation, regulating immunity, stimulating angiogenesis, promoting growth, and enhancing cell differentiation. They are promising for treating these injuries by supporting nerve axon growth and myelin regeneration. This study examines the role of BMSCs in addressing the challenges of spinal cord tissue repair.
Materials and methods: BMSCs were isolated from the femurs of 150–200 g rats, and after culturing, the third passage was used. Thirty Wistar rats (200–300 g) were divided into three groups: control, lesion control, and BMSC-treated. Spinal cord injury was induced by dropping a 10 g weight on the T13–L1 region. On the eighth day after injury, either a vehicle solution or 1 million BMSCs in 10 μL were injected into the injury site. Evaluations, including the BBB test, hot water withdrawal latency, nerve conduction velocity, LFB staining, and GAP-43 protein expression, were performed using the western blot method.
Results: Treatment with BMSCs significantly increased the BBB score, improving hind limb movement in rats. These cells also reduced the reaction time for paw withdrawal and enhanced sensory system function. Furthermore, BMSCs significantly reduced spinal reflex latency and accelerated the compound action potential (CAP) recorded in the gastrocnemius muscle. Moreover, they promoted axon growth and proliferation by increasing GAP-43 protein expression and contributed to improved myelination.
Conclusion: The results indicate that the use of BMSCs as a therapeutic approach reduces axon and myelin degeneration while promoting neuronal growth and repair. These reparative effects at the molecular level were associated with increased GAP-43 protein expression and improved sensory and motor function in the hind limbs of animals.

 

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