Maedeh Entezari Najafabadi, Abbasali Karimpour Malekshah, Amir Esmailnejad Moghaddam, Hatef Ghasemi Hamidabadi, Mahmoud Heidari,
Volume 22, Issue 1 (2-2013)
Abstract
Background and purpose: The purpose of this study was to evaluate the effect of mouse
embryonic fibroblast cells conditioned medium on resumption of meiosis, in vitro maturation of immature
mouse oocytes, fertilization and derived embryos development.
Material and Methods: Immature oocytes denuded at the germinal vesicle (GV) stage were
obtained from female NMRI mice 46-48 hrs after injection of 7/5 IU PMSG. To preparate MEF, the
fetuses were collected from the female mice at day 13 and cultured in DMEM. After the preparation of a
monolayer, conditioned medium were collected and used for culture. Immature oocytes were randomly
cultured in culture medium of MEM-α supplemented with different concentrations (0, 10, 30, 50 and
100%) of mouse embryonic fibroblast conditioned medium. After 14-16 hrs the matured oocytes were
fertilized with spermatozoa in T6 medium and their development was assessed until blastocyst stage.
Results: There was no significant difference in maturation rate between control and conditioned
medium groups. But there were significant differences (P<0.05) in the percentage of fertilization and
cleavage in conditioned medium with 30%-50% concentrations as compared to the control group
(P<0.05).
Conclusion: The conditioned medium of fibroblast cells increase in vitro fertilization (IVF) and
embryo development. But there was no effect on the maturation of immature oocytes.