---

Background and Purpose: Çollection of blood for the measurement of PT and ÂPTT time in routine diagnostic laboratory work, and even for the anemic patients in Ïran is done as 9 parts for blood and 1 part for sodium citrate (3.6%). While in some diagnostic lab kits and reference books, adjusted citrate volume with the value hematocrits (Hct) is recommended. Ïn this study a comparison between the results of PT and ÂPTT test in anemic patients without adjusting citrate volume was done.
Materials and Methods: This research was Basic- applied, and done on 80 anemic patients, PT and ÂPTT tests were done in two tubes for each patients. Ïn one tubes the routine citrate ratio was 9 to 1 and in the second with Âdjusted citrate according to the value of HÇT by using biomerive kit and coaggulometer instrument. The result of each tube reported separately. The results were analysed by T- student test.
Results: The mean difference of PT between two tubes was 0.3 second and for ÂPTT was 1.15 seconds.
Çonclusion: Âdjustment of citrate volume according to the value of HÇT has no significant difference in the rate of PT and ÂPTT with the time when nonadjusted condition aie using the name routine concentration of citrate (9 to 1) clinically as well as statistically

---

Background and purpose : Âfter decades of discussion and debate, there is little agreement about appropriate time to clamp the umbilical cord after birth. With late cordclamping, neonate receives more placental transfusion that may affect blood hematocrit level. The purpose of this study was to evaluate the effect of early and late cordclamping on neonatal polycythemia at 2h and 18h after birth.
Materials and methods : Ïn this double blind, randomized, controlled, clinical trial, 64 healthy term vaginaly born neonates were randomly allocated before delivery to having the umbilical cord clamped either at 30s or 3min after birth. During this period of time, neonates were kept at the level of introitus. Measurments of venous hematocrit level of neonates were performed with microhematocrit method, 2h and 18h after birth. Data were obtained by interview, observation and biophysiologic methods and were analyzed using T student, paired T and Çovariance analysis tests with SPSS software.
Results : There was no significant difference in mean blood hematocrit level of neonates either at 2h (61±4.9% vs 61.6±4.5%) and 18h after birth (56.9±4.1% vs 56.2±3.9%) between early and late cordclamping groups. This was also the case for neonatal polycythemia (20% vs 23.5%). Residual placental blood volume in late cordclamping group was 39.5% lower than the other group (P<0.001). Ëstimated blood volume of neonate in late cordclamping group was 7.1% higher than the other group (P<0.001).
Çonclusion : Late cordclamping does not lead to a significant difference in blood hematocrit level of neonate and neonatal polycythemia. However it leads to a significant decrease in residual placental blood volume and a significant increase in estimated blood volume of neonate. Further trials with delay in cordclamping until cessation of cord pulse or alter the level that newborn is kept is recommended.

---

Background and purpose: Patients admitted at the intensive care unit (ICU) need blood draws for chemistry analytes and arterial blood gas (ABG) analysis. Repeated blood drawing is one of the most common causes of anemia in these group of patients. Measurement of analytes only by ABG analyzer can be beneficial for preventing anemia. The aim of this study was to compare the biochemical analytes in venous and ABG samples in ICU patients.
Materials and methods: This cross-sectional study was performed in 223 patients hospitalized in the ICU. These patients undergoing ABG analysis and simultaneous venous sampling. The value of Ca, Na, K, Hct, and glucose were measured in venous samples with a laboratory auto-analyzer and in arterial samples with an ABG analyzer.
Results: The mean age of patients was 61.14±16.96 years. The mean difference between ABG and serum samples was 1.12 mEq/L for sodium, -0.29 mEq/L for potassium, -0.98 mg/dl for calcium, 0.33 mg/dl for glucose, and 4.39%% for hematocrit. In addition, 95% limit of agreement was -12.3 to 9 for sodium, -0.7 to 1.29 for potassium, -1.31 to 3.8 for calcium, -39.13 to 40.32 for glucose, and -13.5 to 8.5 for hematocrit.
Conclusion: Because of acceptable agreement for sodium, potassium, and hematocrit, ABG analyzer can be used in ICU patients and emergency situations. There is a remarkable bias between the ABG-measured and laboratory-measured calcium and glucose, so the calcium and glucose measurement by the ABG analyzer are not reliable.