Journal of Mazandaran University of Medical Sciences
مجله دانشگاه علوم پزشکی مازندران
J Mazandaran Univ Med Sci
Medical Sciences
http://jmums.mazums.ac.ir
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اثر ضد التهابی عصاره اتانولی گیاه فرفیون
(euphorbia Myrsinites ) در شوک سپتیک القاء شده با
لیپوپلی ساکارید در موش های سوری
Anti-inflammatory Effect of Ethanolic Extract of Euphorbia myrsinites in Lipopolysaccharide-induced
Septic Shock in Mice
ايمونولوژي
Immunology
پژوهشي-کامل
Research(Original)
<div dir="rtl"><span style="letter-spacing:-.1pt;"><span style="color:black;"><span style="font-family:b koodak;">سابقه</span></span></span><span style="letter-spacing:-.1pt;"><span style="color:black;"><span style="font-family:b koodak;"> و هدف:</span></span></span><span style="background:white;"><span style="font-family:b zar;"> بسیاری از گیاهان دارای خواص </span></span><span style="background:white;"><span style="font-family:b zar;">ایمونومدولاتوری</span></span> <span style="background:white;"><span style="font-family:b zar;">هستند که از این میان می</span></span><span style="font-family:b zar;">‏<span style="background:white;">توان جنس </span></span><span dir="LTR" style="background:white;"><span style="font-size:10.0pt;">Euphorbia</span></span><span style="background:white;"><span style="font-family:b zar;"> را به عنوان یک </span></span><span style="background:white;"><span style="font-family:b zar;">گیاه تعدیل کننده سیستم ایمنی نام برد. هدف از این مطالعه ارزیابی اثرات</span></span><span style="background:white;"><span style="font-family:b zar;"> ضد التهابی عصاره اتانولی گیاه فرفیون (</span></span><em><span dir="LTR" style="background:white;"><span style="font-size:10.0pt;">E.myrsinites</span></span></em><span style="background:white;"><span style="font-family:b zar;">) بود.</span></span><span style="letter-spacing:-.1pt;"><span style="color:black;"><span style="font-family:b zar;"></span></span></span><br>
<span style="letter-spacing:-.1pt;"><span style="color:black;"><span style="font-family:b koodak;">مواد و روش</span></span></span><span style="letter-spacing:-.1pt;"><span style="color:black;"><span style="font-family:b zar;"></span></span></span><span style="letter-spacing:-.1pt;"><span style="color:black;"><span style="font-family:b koodak;">ها:</span></span></span> <span style="background:white;"><span style="font-family:b zar;">در این مطالعه ی تجربی، 4</span></span><span style="background:white;"><span style="font-family:b zar;">0 موش نژاد</span></span><span dir="LTR" style="background:white;"><span style="font-size:10.0pt;"> Swiss albino </span></span><span style="background:white;"><span style="font-family:b zar;">به 4 گروه مساوی تقسیم شدند. گروه </span></span><span dir="LTR" style="background:white;"><span style="font-size:10.0pt;">A</span></span><span style="background:white;"><span style="font-family:b zar;"> (گروه کنترل)، گروه </span></span><span dir="LTR" style="background:white;"><span style="font-size:10.0pt;">B</span></span><span style="background:white;"><span style="font-family:b zar;"> (تحت تجویز با </span></span><span style="letter-spacing:-.1pt;"><span style="font-family:b zar;">لیپوپلی ساکارید باکتریایی یا </span></span><span dir="LTR" style="letter-spacing:-.1pt;"><span style="font-size:10.0pt;">LPS</span></span><span style="background:white;"><span style="font-family:b zar;">)</span></span> <span style="background:white;"><span style="font-family:b zar;">و گروه </span></span><span dir="LTR" style="background:white;"><span style="font-size:10.0pt;">C</span></span><span style="background:white;"><span style="font-family:b zar;"> (دریافت ع</span></span><span style="background:white;"><span style="font-family:b zar;">صاره اتانولی گیا</span></span><span style="background:white;"><span style="font-family:b zar;">ه </span></span><span dir="LTR" style="background:white;"><span style="font-size:10.0pt;">E.Myrsinite</span></span><span style="background:white;"><span style="font-family:b zar;">، قبل از تجویز </span></span><span dir="LTR" style="background:white;"><span style="font-size:10.0pt;">LPS</span></span><span style="background:white;"><span style="font-family:b zar;">) گروه </span></span><span dir="LTR" style="background:white;"><span style="font-size:10.0pt;">D</span></span><span style="background:white;"><span style="font-family:b zar;"> (دریافت عصاره اتانولی گیاه مورد نظر همراه با تجویز </span></span><span dir="LTR" style="background:white;"><span style="font-size:10.0pt;">LPS</span></span><span style="background:white;"><span style="font-family:b zar;">)</span></span><span style="background:white;"><span style="font-family:b zar;">. در نهایت از موش</span></span><span style="font-family:b zar;">‏<span style="background:white;">ها خونگیری شد (</span></span><span style="background:white;"><span style="font-family:b zar;">2 و 6 ساعت پس از تجویز </span></span><span dir="LTR" style="background:white;"><span style="font-size:10.0pt;">LPS</span></span><span style="background:white;"><span style="font-family:b zar;">)</span></span><span style="background:white;"><span style="font-family:b zar;"> و به بررسی سایتوکاین</span></span><span style="font-family:b zar;">‏<span style="background:white;">های </span></span><span dir="LTR" style="background:white;"><span style="font-size:10.0pt;">IFN-γ</span></span><span style="background:white;"><span style="font-family:b zar;"> و </span></span><span dir="LTR" style="background:white;"><span style="font-size:10.0pt;">TNF-α</span></span><span style="background:white;"><span style="font-family:b zar;">، </span></span><span dir="LTR" style="background:white;"><span style="font-size:10.0pt;">IL-4</span></span> <span style="background:white;"><span style="font-family:b zar;">و </span></span><span dir="LTR" style="background:white;"><span style="font-size:10.0pt;">IL-10</span></span><span style="background:white;"><span style="font-family:b zar;"> و نیتریک اکساید پرداخته شد.</span></span><span style="color:black;"><span style="font-family:b zar;"></span></span><br>
<span style="letter-spacing:-.2pt;"><span style="color:black;"><span style="font-family:b koodak;">یافته</span></span></span><span style="letter-spacing:-.2pt;"><span style="color:black;"><span style="font-family:b zar;"></span></span></span><span style="letter-spacing:-.2pt;"><span style="color:black;"><span style="font-family:b koodak;">ها:</span></span></span> <span style="letter-spacing:-.1pt;background:white;"><span style="font-family:b zar;">2 ساعت بعد از تجویز </span></span><span dir="LTR" style="letter-spacing:-.1pt;background:white;"><span style="font-size:10.0pt;">LPS</span></span><span style="letter-spacing:-.1pt;background:white;"><span style="font-family:b zar;">، میزان </span></span><span dir="LTR" style="letter-spacing:-.1pt;background:white;"><span style="font-size:10.0pt;">IL-4</span></span><span style="letter-spacing:-.1pt;background:white;"><span style="font-family:b zar;"> و </span></span><span dir="LTR" style="letter-spacing:-.1pt;background:white;"><span style="font-size:10.0pt;">IL-10</span></span> <span style="letter-spacing:-.1pt;background:white;"><span style="font-family:b zar;">در گروه </span></span><span dir="LTR" style="letter-spacing:-.1pt;background:white;"><span style="font-size:10.0pt;">C</span></span><span style="letter-spacing:-.1pt;background:white;"><span style="font-family:b zar;"> و </span></span><span dir="LTR" style="letter-spacing:-.1pt;background:white;"><span style="font-size:10.0pt;">D</span></span><span style="letter-spacing:-.1pt;background:white;"><span style="font-family:b zar;"> نسبت به گروه </span></span><span dir="LTR" style="letter-spacing:-.1pt;background:white;"><span style="font-size:10.0pt;">B</span></span> <span style="letter-spacing:-.1pt;background:white;"><span style="font-family:b zar;">افزایش</span></span><span style="letter-spacing:-.1pt;background:white;"><span style="font-family:b zar;"> معنی</span></span><span style="letter-spacing:-.1pt;"><span style="font-family:b zar;">‏</span></span><span style="letter-spacing:-.1pt;background:white;"><span style="font-family:b zar;">دار</span></span><span style="letter-spacing:-.1pt;background:white;"><span style="font-family:b zar;"> و میزان</span></span> <span dir="LTR" style="letter-spacing:-.2pt;background:white;"><span style="font-size:10.0pt;">IFN</span></span><span dir="LTR" style="background:white;"><span style="font-size:10.0pt;">-γ</span></span><span style="background:white;"><span style="font-family:b zar;"> در این گروه</span></span><span style="font-family:b zar;">‏</span><span style="background:white;"><span style="font-family:b zar;">ها کاهش معنی</span></span><span style="font-family:b zar;">‏</span><span style="background:white;"><span style="font-family:b zar;">دار داشت (0425/0=</span></span><span dir="LTR" style="background:white;"><span style="font-size:10.0pt;">P</span></span><span style="background:white;"><span style="font-family:b zar;">)</span></span><span style="background:white;"><span style="font-family:b zar;">، اما 6 ساعت بعد از تجویز </span></span><span dir="LTR" style="background:white;"><span style="font-size:10.0pt;">LPS</span></span><span style="background:white;"><span style="font-family:b zar;">، میزان </span></span><span dir="LTR" style="background:white;"><span style="font-size:10.0pt;">TNF-α</span></span><span style="background:white;"><span style="font-family:b zar;"> در گروه </span></span><span dir="LTR" style="background:white;"><span style="font-size:10.0pt;">B</span></span><span style="background:white;"><span style="font-family:b zar;"> <span style="letter-spacing:-.25pt;">نسبت به گروه </span></span></span><span dir="LTR" style="letter-spacing:-.25pt;background:white;"><span style="font-size:10.0pt;">C</span></span><span style="letter-spacing:-.25pt;background:white;"><span style="font-family:b zar;"> و </span></span><span dir="LTR" style="letter-spacing:-.25pt;background:white;"><span style="font-size:10.0pt;">D</span></span><span style="letter-spacing:-.25pt;background:white;"><span style="font-family:b zar;"> افزایش معنی</span></span><span style="letter-spacing:-.25pt;"><span style="font-family:b zar;">‏</span></span><span style="letter-spacing:-.25pt;background:white;"><span style="font-family:b zar;">دار داشت (0473/0=</span></span><span dir="LTR" style="letter-spacing:-.25pt;background:white;"><span style="font-size:10.0pt;">P</span></span><span style="letter-spacing:-.25pt;background:white;"><span style="font-family:b zar;">)</span></span> <span style="letter-spacing:-.25pt;background:white;"><span style="font-family:b zar;">ولی در ارتباط با میزان </span></span><span dir="LTR" style="letter-spacing:-.25pt;background:white;"><span style="font-size:10.0pt;"> IL-4</span></span><span style="letter-spacing:-.25pt;background:white;"><span style="font-family:b zar;">و</span></span><span dir="LTR" style="letter-spacing:-.25pt;background:white;"><span style="font-size:10.0pt;"> IL-10 </span></span><span style="letter-spacing:-.25pt;background:white;"><span style="font-family:b zar;">نتیجه عکس بود</span></span><span style="letter-spacing:-.25pt;background:white;"><span style="font-family:b zar;">.</span></span> <span style="letter-spacing:-.25pt;"><span style="font-family:b zar;">سطح نیتریک</span></span> <span style="font-family:b zar;">اکساید، </span><span style="font-family:b zar;">2 و6 ساعت پس ازتجویز</span><span dir="LTR"><span style="font-size:10.0pt;">LPS</span></span><span style="font-family:b zar;">، </span><span style="font-family:b zar;">در موش‏های</span> <span style="font-family:b zar;">گروه </span><span dir="LTR"><span style="font-size:10.0pt;">C</span></span><span style="font-family:b zar;"> و</span><span dir="LTR"><span style="font-size:10.0pt;">D</span></span><span style="font-family:b zar;"> نسبت به گروه </span><span dir="LTR"><span style="font-size:10.0pt;">B</span></span><span style="font-family:b zar;"> کاهش <span style="background:white;">معنی</span></span><span style="font-family:b zar;">‏</span><span style="background:white;"><span style="font-family:b zar;">دار</span></span><span style="font-family:b zar;"> داشت (0421/0</span><span dir="LTR"><span style="font-size:10.0pt;">=</span></span> <span dir="LTR"><span style="font-size:10.0pt;">P</span></span><span style="font-family:b zar;">).<span style="color:black;"></span></span><br>
<span style="letter-spacing:-.2pt;"><span style="color:black;"><span style="font-family:b koodak;">استنتاج:</span></span></span><span style="background:white;"><span style="font-family:b zar;"> عصاره اتانولی گیاه </span></span><em><span dir="LTR" style="background:white;"><span style="font-size:10.0pt;">E.myrsinites</span></span></em> <span style="background:white;"><span style="font-family:b zar;">دارای اثرات تعدیل</span></span><span style="font-family:b zar;">‏</span><span style="background:white;"><span style="font-family:b zar;">کننده سیستم ایمنی است و از طریق کاهش تولید سایتوکاین</span></span><span style="font-family:b zar;">‏</span><span style="background:white;"><span style="font-family:b zar;">های پیش التهابی می</span></span><span style="font-family:b zar;">‏</span><span style="background:white;"><span style="font-family:b zar;">تواند به عنوان داروی موثر در درمان بیماری</span></span><span style="font-family:b zar;">‏</span><span style="background:white;"><span style="font-family:b zar;">های التهابی چون شوک سپتیک استفاده شود.</span></span><span style="letter-spacing:-.2pt;"><span style="color:black;"><span style="font-family:b zar;"></span></span></span></div>
<strong>Background and purpose:</strong> Many herbs including <em>Euphorbia</em> have immunomodulatory properties. The aim of this study was to evaluate the immunomodulatory effects of the ethanolic extract of <em>E.myrsinites</em>.<br>
<strong>Materials and methods:</strong> In this experimental study, 40 Swiss albino male mice were randomly divided into 4 groups. Group A included healthy mice, group B was induced by lipopolysaccharide (LPS), group C received the ethanolic extract of <em>E.myrsinites</em> 7 days before LPS induction. Group D received the plant extract at the same time with LPS induction. Then, blood samples were collected (2 and 6 h after LPS induction) and IL-4, IL-10, TNF-α, and IFN-γ and nitric oxide production were evaluated.<br>
<strong>Results:</strong> Two hours after LPS induction, the levels of IL-4 and IL-10 in groups C and D increased significantly and IFN-γ level decreased significantly compared to those in group B (P=0.0425). But, six hours after LPS induction, TNF-α level significantly increased in group B compared to that in groups C and D (P=0.0473), but IL-4 and IL-10 levels increased significantly in groups C and D compared to those in group B (P=0.0411). The levels of nitric oxide, 2 and 6 hours after LPS induction, significantly decreased in groups C and D compared to those in group B (P=0.0421).<br>
<strong>Conclusion:</strong> <em>E.myrsinites</em> ethanolic extract has immunomodulatory effects and could be used as an effective drug in inflammatory diseases such as septic shock through reduced production of pro-inflammatory cytokines.
گیاه فرفیون, سایتوکاینهای پیش التهابی/ ضد التهابی, لیپو پلی ساکارید, موش, نیتریک اکساید.
Euphorbia myrsinitis, pro-inflammatory/anti-inflammatory cytokines, LPS, mice, nitric oxide
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http://jmums.mazums.ac.ir/browse.php?a_code=A-10-9086-2&slc_lang=fa&sid=1
Somayyeh
Ahmadi Afshar
سمیه
احمدی افشار
afsharnegin92@gmail.com