Journal of Mazandaran University of Medical Sciences
مجله دانشگاه علوم پزشکی مازندران
J Mazandaran Univ Med Sci
Medical Sciences
http://jmums.mazums.ac.ir
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admin
1735-9260
1735-9279
fa
jalali
1401
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1
gregorian
2023
3
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32
218
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ارزیابی بیان RNAs غیر کدکننده طویل MALAT-1 و
HOTAIR در بروز بیماری هموفیلی
Evaluation of MALAT-1 and HOTAIR LncRNAs Expression
Levels in Incidence of Hemophilia
اپیدمیولوژی
Epidemiology
پژوهشي-کامل
Research(Original)
<span style="font-size:12px;"><span style="line-height: 3;"><span style="line-height:2;"><span style="direction: rtl;"><span style="unicode-bidi: embed;"><span new="" roman="" times=""><span lang="FA"><span b="" koodak=""><span style="color: black;"><span style="letter-spacing: -0.1pt;">سابقه</span></span></span></span><span lang="AR-SA"><span b="" koodak=""><span style="color: black;"><span style="letter-spacing: -0.1pt;"> و هدف:</span></span></span></span> <span dir="LTR">RNA</span><span lang="FA"><span b="" zar=""><span style="color: black;"></span></span></span><span lang="AR-SA"><span b="" zar="">های غیرکدکننده طویل (</span></span><span dir="LTR">LncRNAs</span><span lang="AR-SA"><span b="" zar="">) حاوی بیش از 200 نوکلئوتید هستند</span></span><span lang="FA"><span b="" zar=""> که در تنظیم رونویسی و پس از رونویسی بیان ژن نقش دارند</span></span><span lang="AR-SA"><span b="" zar=""> و در تشخیص، پیشگیری و درمان اختلالاتی نظیر سرطان</span></span><span lang="FA"><span b="" zar=""><span style="color: black;"></span></span></span><span lang="AR-SA"><span b="" zar="">ها مورد بررسی قرار میگیرند. به هر حال، ارزیابی آنها در بیماری هموفیلی به میزان کمی انجام شده است. بنابراین</span></span> <span lang="AR-SA"><span b="" zar="">هدف</span></span> <span lang="AR-SA"><span b="" zar="">مطالعه</span></span> <span lang="AR-SA"><span b="" zar="">حاضر شناسایی و تعیین ارتباط بین سطوح بیان دو </span></span><span dir="LTR">LncRNA</span><span b="" zar=""> رایج (</span><span dir="LTR"><span style="letter-spacing: -0.2pt;">MALAT-1 and HOTAIR</span></span><span lang="AR-SA"><span b="" zar="">) و بروز هموفیلی </span></span><span dir="LTR">A</span><span lang="AR-SA"><span b="" zar=""> در بیماران مرد ایرانی بود.</span></span><span lang="FA"><span b="" zar=""><span style="color:#0e101a"></span></span></span></span></span></span><br>
<span style="direction: rtl;"><span style="unicode-bidi: embed;"><span new="" roman="" times=""><span lang="AR-SA"><span b="" koodak=""><span style="color: black;"><span style="letter-spacing: -0.1pt;">مواد و روش</span></span></span></span><span lang="FA"><span b="" zar=""><span style="color: black;"><span style="letter-spacing: -0.1pt;"></span></span></span></span><span lang="AR-SA"><span b="" koodak=""><span style="color: black;"><span style="letter-spacing: -0.1pt;">ها</span></span></span></span><span lang="AR-SA"><span b="" zar=""><span style="color: black;"><span style="letter-spacing: -0.1pt;">:</span></span></span></span> <span lang="FA"><span b="" zar="">در این مطالعه مورد/شاهدی،</span></span> <span lang="FA"><span b="" zar=""><span style="letter-spacing: -0.1pt;">دو </span></span></span><span dir="LTR"><span style="letter-spacing: -0.1pt;">LncRNA</span></span><span lang="FA"><span b="" zar=""><span style="letter-spacing: -0.1pt;"> رایج (</span></span></span><span dir="LTR"><span style="letter-spacing: -0.1pt;">HOTAIR</span></span><span lang="FA"><span b="" zar=""><span style="letter-spacing: -0.1pt;"> و </span></span></span><span dir="LTR"><span style="letter-spacing: -0.1pt;">MALAT-1</span></span><span lang="FA"><span b="" zar=""><span style="letter-spacing: -0.1pt;">) بر اساس ویژگیهایشان، برای تجزیه و تحلیل بیان ژن با استفاده از روش کمی </span></span></span><span dir="LTR"><span style="letter-spacing: -0.1pt;">Real-time PCR</span></span><span lang="FA"><span b="" zar=""><span style="letter-spacing: -0.1pt;"> انتخاب شدند. در ابتدا، 5 میلی</span></span></span><span lang="FA"><span b="" zar=""><span style="color: black;"></span></span></span><span lang="FA"><span b="" zar=""><span style="letter-spacing: -0.1pt;">لیتر خون تام در لوله حاوی ضد انعقاد </span></span></span><span dir="LTR"><span style="letter-spacing: -0.1pt;">EDTA</span></span><span lang="FA"><span b="" zar=""><span style="letter-spacing: -0.1pt;"> از 50 بیمار مرد با هموفیلی شدید </span></span></span><span dir="LTR"><span style="letter-spacing: -0.1pt;">A</span></span><span lang="FA"><span b="" zar=""><span style="letter-spacing: -0.1pt;"> و 50 مرد سالم جمع</span></span></span><span lang="FA"><span b="" zar=""><span style="color: black;"></span></span></span><span lang="FA"><span b="" zar=""><span style="letter-spacing: -0.1pt;">آوری شد. سپس، </span></span></span><span dir="LTR"><span style="letter-spacing: -0.1pt;">RNA</span></span><span lang="FA"><span b="" zar=""><span style="letter-spacing: -0.1pt;"> تام از خون محیطی استخراج و </span></span></span><span dir="LTR"><span style="letter-spacing: -0.1pt;">cDNA</span></span><span b="" zar=""><span style="letter-spacing: -0.1pt;"> توسط روش رونویسی معکوس سنتز شد. بعد از آن، بیان </span></span><span dir="LTR"><span style="letter-spacing: -0.1pt;">LncRNA</span></span><span lang="FA"><span b="" zar=""><span style="letter-spacing: -0.1pt;"> های انتخاب شده با استفاده از </span></span></span><span dir="LTR"><span style="letter-spacing: -0.1pt;">qRT-PCR</span></span><span lang="FA"><span b="" zar=""><span style="letter-spacing: -0.1pt;"> مورد تجزیه و تحلیل قرار گرفت. نهایتا، نسبتهای بیان ژن برای </span></span></span><span dir="LTR"><span style="letter-spacing: -0.1pt;">LncRNA</span></span><span lang="FA"><span b="" zar=""><span style="color: black;"></span></span></span><span lang="FA"><span b="" zar=""><span style="letter-spacing: -0.1pt;">ها توسط نرمافزار </span></span></span><span dir="LTR"><span style="letter-spacing: -0.1pt;">REST-2009</span></span><span lang="FA"><span b="" zar=""><span style="letter-spacing: -0.1pt;"> تجزیه و تحلیل شدند.</span></span></span><span lang="FA"><span b="" zar=""><span style="color:black"></span></span></span></span></span></span><br>
<span style="direction: rtl;"><span style="unicode-bidi: embed;"><span new="" roman="" times=""><span lang="AR-SA"><span b="" koodak=""><span style="color: black;"><span style="letter-spacing: -0.2pt;">یافته</span></span></span></span><span lang="FA"><span b="" zar=""><span style="color: black;"><span style="letter-spacing: -0.2pt;"></span></span></span></span><span lang="AR-SA"><span b="" koodak=""><span style="color: black;"><span style="letter-spacing: -0.2pt;">ها:</span></span></span></span> <span lang="FA"><span b="" zar="">مقایسه بین گروههای هموفیل و غیرهموفیل (سالم) نشان داد که میانگین سطوح بیان دو </span></span><span dir="LTR">LncRNA</span><br>
<span lang="FA"><span b="" zar="">انتخاب شده (</span></span><span dir="LTR">HOTAIR</span><span lang="FA"><span b="" zar=""> و </span></span><span dir="LTR">MALAT-1</span><span lang="FA"><span b="" zar="">) در نمونه<span style="color: black;"></span>های هموفیلی </span></span><span dir="LTR">A</span><span b="" zar=""> در مقایسه با نمونه های نرمال،</span> <span lang="AR-SA"><span b="" zar="">ب</span></span><span lang="FA"><span b="" zar=""><span style="color: black;">ه</span></span></span><span lang="AR-SA"><span b="" zar="">طور</span></span> <span lang="FA"><span b="" zar="">قابل توجهی</span></span> <span lang="FA"><span b="" zar="">پایین<span style="color: black;"></span>تر بود (0/05</span></span><span lang="FA"><span b="" zar="">></span></span><span dir="LTR">P</span><span lang="FA"><span b="" zar="">).<span style="color:black"></span></span></span></span></span></span></span><br>
<span style="direction: rtl;"><span style="unicode-bidi: embed;"><span new="" roman="" times=""><span style="line-height:2;"><span lang="AR-SA"><span b="" koodak=""><span style="color: black;"><span style="letter-spacing: -0.2pt;">استنتاج:</span></span></span></span> </span><span lang="FA"><span b="" zar=""><span style="letter-spacing: -0.1pt;"><span style="line-height:2;">میزان بیان پایی</span>ن </span></span></span><span dir="LTR"><span style="letter-spacing: -0.1pt;">LncRNA</span></span><span lang="FA"><span b="" zar=""><span style="color: black;"></span></span></span><span lang="FA"><span b="" zar=""><span style="letter-spacing: -0.1pt;">های </span></span></span><span dir="LTR">HOTAIR</span><span lang="FA"><span b="" zar=""> و </span></span><span dir="LTR">MALAT-1</span><span lang="FA"><span b="" zar=""> ممکن است با بروز بیماری <span style="letter-spacing: -0.1pt;">در بیماران مرد هموفیلی </span></span></span><span dir="LTR"><span style="letter-spacing: -0.1pt;">A</span></span><span lang="FA"><span b="" zar=""><span style="letter-spacing: -0.1pt;"> ارتباط داشته باشد.</span></span></span><span lang="AR-SA"><span b="" zar=""><span style="color: black;"><span style="letter-spacing:-.2pt"></span></span></span></span></span></span></span><br>
</span></span>
<span style="font-size:12pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:10.0pt"><span style="line-height:150%"><span arial="" style="font-family:"><span style="letter-spacing:-.2pt">Background and purpose:</span></span></span></span></b> <span style="font-size:10.0pt"><span style="line-height:150%"><span style="letter-spacing:-.2pt">LncRNAs are a group of non-coding RNAs with more than 200 nucleotides involved in transcriptional and post-transcriptional regulation of gene expression, that have a potential role in diagnosis, prevention and treatment of some disorders such as cancers. There is paucity of information about their involvement in hemophilia disease. This study aimed at discovering the relationship between expression levels of two common LncRNAs (MALAT-1 and HOTAIR) and incidence of hemophilia A in Iranian male patients.</span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:150%"><span style="text-autospace:none"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:10.0pt"><span style="line-height:150%"><span arial="" style="font-family:">Materials and methods:</span></span></span></b><b> </b><span style="font-size:10.0pt"><span style="line-height:150%">In this case-control study two common LncRNAs (HOTAIR and MALAT-1) were selected for gene expression analysis using quantitative Real-time PCR based on their features. At first, 5 ml of whole blood in EDTA anti-coagulant tube was collected from 50 severe hemophilia A male patients and 50 healthy male donors. Then, total RNA was extracted from peripheral blood and the cDNA was synthesized by reverse transcription method. After that, the expression of the selected LncRNAs was analyzed using qRT-PCR. Finally,</span></span> <span style="font-size:10.0pt"><span style="line-height:150%">the gene expression ratios for the LncRNAs were analyzed using REST-2009.</span></span></span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:10.0pt"><span style="line-height:150%"><span arial="" style="font-family:">Results:</span></span></span></b><b> </b><span style="font-size:10.0pt"><span style="line-height:150%"><span style="letter-spacing:-.1pt">A comparison between the hemophilia and non-hemophilia (healthy) groups demonstrated</span></span></span><span style="font-size:10.0pt"><span style="line-height:150%"> that the mean expression levels of two selected LncRNAs </span></span><span style="font-size:10.0pt"><span style="line-height:150%"><span style="letter-spacing:-.2pt">(MALAT-1 and HOTAIR) </span></span></span><span style="font-size:10.0pt"><span style="line-height:150%">were significantly lower in hemophilia A samples compared with normal samples (P< 0.05).</span></span><span lang="EN" style="font-size:10.0pt"><span style="line-height:150%"></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="line-height:150%"><span style="vertical-align:top"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:10.0pt"><span style="line-height:150%"><span arial="" style="font-family:">Conclusion:</span></span></span></b> <span style="font-size:10.0pt"><span style="line-height:150%">The low expression levels of HOTAIR and MALAT-1 LncRNAs may be associated with incidence of disease in hemophilia A male patients.</span></span><i><span style="font-size:10.0pt"><span style="line-height:150%"></span></span></i></span></span></span></span><br>
RNA غیر کد کننده طویل, MALAT-1,HOTAIR , هموفیلی, فاکتور 8
Long Non Coding RNA, MALAT-1, HOTAIR, hemophilia, factor VIII
1
12
http://jmums.mazums.ac.ir/browse.php?a_code=A-10-15843-1&slc_lang=fa&sid=1
Elnaz
Agi
الناز
آگی
elnazagi@yahoo.com