Journal of Mazandaran University of Medical Sciences
مجله دانشگاه علوم پزشکی مازندران
J Mazandaran Univ Med Sci
Medical Sciences
http://jmums.mazums.ac.ir
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1735-9260
1735-9279
fa
jalali
1405
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2026
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بررسی اثر تریاک بر پروفایل لیپیدی و تغییرات بافت شناسی کبد در رتهای دریافت کننده رژیم غنی از کلسترول
Investigation of Opium-induced Changes in Lipid Profile and Hepatic Histopathology in Rats Receiving a Cholesterol-rich Diet
بيولوژي
Biology
پژوهشي-کامل
Research(Original)
<span style="line-height:2;"><span style="font-size:12pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA"><span b="" koodak="" style="font-family:"><span style="letter-spacing:-.2pt">سابقه و هدف:</span></span></span></b> <span b="" lang="AR-SA" style="font-family:" zar="">سوء مصرف مواد مخدر </span><span lang="AR-SA"><span b="" style="font-family:" zar="">امروزه یکی از رایج</span></span><span b="" lang="AR-SA" style="font-family:" zar=""></span><span lang="AR-SA"><span b="" style="font-family:" zar="">ترین تهدیدها برای انسان است که می</span></span><span b="" lang="AR-SA" style="font-family:" zar=""></span><span lang="AR-SA"><span b="" style="font-family:" zar="">تواند منجر به آسیب کبدی و حتی مرگ زود هنگام شود.</span></span><span lang="AR-BH"><span b="" style="font-family:" zar=""> مطالعه حاضر با هدف بررسی اثر تریاک بر پروفایل لیپیدی و تغییرات بافت کبد</span></span> <span lang="AR-BH"><span b="" style="font-family:" zar="">در رت های دریافت کننده رژیم غنی از کلسترول، انجام پذیرفت.</span></span><span lang="FA"><span b="" roya="" style="font-family:"><span style="letter-spacing:-.2pt"></span></span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="AR-SA"><span b="" koodak="" style="font-family:"><span style="letter-spacing:-.2pt">مواد و روش </span></span></span></b><b><span lang="FA"><span b="" koodak="" style="font-family:"><span style="letter-spacing:-.2pt"></span></span></span></b><b><span lang="AR-SA"><span b="" koodak="" style="font-family:"><span style="letter-spacing:-.2pt">ها:</span></span></span></b> <span lang="AR-BH"><span b="" style="font-family:" zar="">در این مطالعه تجربی، 24 عدد رت ویستار با میانگین وزنی 220-200 گرم در 4 گروه 6 تایی قرار گرفتند. جهت القای اعتیاد در رت</span></span><span b="" lang="AR-SA" style="font-family:" zar=""></span><span lang="AR-BH"><span b="" style="font-family:" zar="">ها، تریاک به مدت چهار هفته روزانه دو نوبت (صبح و بعد از ظهر) </span></span><span lang="AR-SA"><span b="" style="font-family:" zar="">در 1 میلی</span></span><span b="" lang="AR-SA" style="font-family:" zar=""></span><span lang="AR-SA"><span b="" style="font-family:" zar="">لیتر آب داغ حل شد و پس از سرد شدن به صورت گاواژ به رت</span></span><span b="" lang="AR-SA" style="font-family:" zar=""></span><span lang="AR-SA"><span b="" style="font-family:" zar="">ها خورانده شد. در پایان مطالعه</span></span><span lang="AR-BH"><span b="" style="font-family:" zar=""> نمونه سرم جمع آوری شد و بافت کبد از تمام حیوانات جدا شد. پروفایل لیپیدی در سرم رت ها به وسیله روش رنگ سنجی اندازه گیری شد. هم</span></span><span b="" lang="AR-SA" style="font-family:" zar=""></span><span lang="AR-BH"><span b="" style="font-family:" zar="">چنین رنگ آمیزی هماتوکسیلین- ائوزین جهت بررسی هیستوپاتولوژی بر روی نمونه</span></span><span b="" lang="AR-SA" style="font-family:" zar=""></span><span lang="AR-BH"><span b="" style="font-family:" zar="">های بافت کبد انجام شد. داده</span></span><span b="" lang="AR-SA" style="font-family:" zar=""></span><span lang="AR-BH"><span b="" style="font-family:" zar="">ها با استفاده از نرم</span></span><span b="" lang="AR-SA" style="font-family:" zar=""></span><span lang="AR-BH"><span b="" style="font-family:" zar="">افزار </span></span><span dir="LTR" style="font-size:10.0pt">SPSS</span><span lang="AR-SA"><span b="" style="font-family:" zar=""> آنالیز و بر اساس میانگین </span></span><span dir="LTR" style="font-size:10.0pt">Mean ±SEM</span><span lang="AR-SA"><span b="" style="font-family:" zar=""> گزارش شدند.</span></span><span lang="FA"><span b="" style="font-family:" zar=""><span style="letter-spacing:-.2pt"></span></span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="AR-SA"><span b="" koodak="" style="font-family:"><span style="letter-spacing:-.2pt">یافته</span></span></span></b><b><span lang="FA"><span b="" koodak="" style="font-family:"><span style="letter-spacing:-.2pt"></span></span></span></b><b><span lang="AR-SA"><span b="" koodak="" style="font-family:"><span style="letter-spacing:-.2pt">ها:</span></span></span></b> <span lang="AR-BH"><span b="" style="font-family:" zar="">نتایج این مطالعه نشان می</span></span><span b="" lang="AR-SA" style="font-family:" zar=""></span><span lang="AR-BH"><span b="" style="font-family:" zar="">دهد که تریاک سبب کاهش سطح تری</span></span><span b="" lang="AR-SA" style="font-family:" zar=""></span><span lang="AR-BH"><span b="" style="font-family:" zar="">گلیسرید (0/0019</span></span><span dir="LTR" style="font-size:10.0pt">P=</span><span lang="AR-BH"><span b="" style="font-family:" zar="">)، </span></span><span dir="LTR" style="font-size:10.0pt">HDL-C</span><span lang="AR-BH"><span b="" style="font-family:" zar=""> (0/0004</span></span><span dir="LTR" style="font-size:10.0pt">P=</span><span lang="AR-BH"><span b="" style="font-family:" zar="">) و </span></span><span dir="LTR" style="font-size:10.0pt">VLDL-C</span><span b="" style="font-family:" zar=""> (0/0019</span><span dir="LTR" style="font-size:10.0pt">P=</span><span lang="AR-BH"><span b="" style="font-family:" zar="">) در گروه معتاد به تریاک نسبت به گروه کنترل سالم شد (0/05 </span></span><span dir="LTR" style="font-size:10.0pt">P<</span><span lang="AR-BH"><span b="" style="font-family:" zar="">). هم</span></span><span b="" lang="AR-SA" style="font-family:" zar=""></span><span lang="AR-BH"><span b="" style="font-family:" zar="">چنین با مطالعات بافت شناسی مشخص شد که این ماده مخدر در بافت کبد سبب بی</span></span><span b="" lang="AR-SA" style="font-family:" zar=""></span><span lang="AR-BH"><span b="" style="font-family:" zar="">نظمی، تغییر شکل و تخریب سلول</span></span><span b="" lang="AR-SA" style="font-family:" zar=""></span><span lang="AR-BH"><span b="" style="font-family:" zar="">ها و ارتشاح سلول های لنفاوی می</span></span><span b="" lang="AR-SA" style="font-family:" zar=""></span><span lang="AR-BH"><span b="" style="font-family:" zar="">شود.</span></span><span lang="AR-SA"><span b="" style="font-family:" zar=""><span style="letter-spacing:-.2pt"></span></span></span></span></span></span></span><br>
<b><span dir="RTL" lang="FA" style="font-size:12.0pt"><span b="" koodak="" style="font-family:"><span style="letter-spacing:-.1pt">استنتاج:</span></span></span></b> <span dir="RTL" lang="AR-SA" style="font-size:12.0pt"><span b="" style="font-family:" zar="">اگر چه تریاک سبب </span></span><span dir="RTL" lang="AR-BH" style="font-size:12.0pt"><span b="" style="font-family:" zar="">کاهش لیپید می</span></span><span dir="RTL" lang="AR-SA" style="font-size:12.0pt"><span b="" style="font-family:" zar=""></span></span><span dir="RTL" lang="AR-BH" style="font-size:12.0pt"><span b="" style="font-family:" zar="">شود و دارای برخی اثرات مفید بر بدن می</span></span><span dir="RTL" lang="AR-SA" style="font-size:12.0pt"><span b="" style="font-family:" zar=""></span></span><span dir="RTL" lang="AR-BH" style="font-size:12.0pt"><span b="" style="font-family:" zar="">باشد، اما با توجه به یافته</span></span><span dir="RTL" lang="AR-SA" style="font-size:12.0pt"><span b="" style="font-family:" zar=""></span></span><span dir="RTL" lang="AR-BH" style="font-size:12.0pt"><span b="" style="font-family:" zar="">های بافت</span></span><span dir="RTL" lang="AR-SA" style="font-size:12.0pt"><span b="" style="font-family:" zar=""></span></span><span dir="RTL" lang="AR-BH" style="font-size:12.0pt"><span b="" style="font-family:" zar="">شناسی این مطالعه تریاک می</span></span><span dir="RTL" lang="AR-SA" style="font-size:12.0pt"><span b="" style="font-family:" zar=""></span></span><span dir="RTL" lang="AR-BH" style="font-size:12.0pt"><span b="" style="font-family:" zar="">تواند اثرات مخربی بر بافت کبد داشته باشد.</span></span></span>
<span style="line-height:2;"><span style="font-size:12pt"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:10.0pt"><span arial="" style="font-family:"><span style="letter-spacing:-.1pt">Background and purpose:</span></span></span></b> <span style="font-size:10.0pt">Drug abuse is one of the most significant public health threats worldwide and may lead to liver damage and premature mortality. This study aimed to investigate the effects of opium on lipid profile and hepatic histopathological changes in rats receiving a high-fat diet.</span></span></span><br>
<span style="font-size:12pt"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:10.0pt"><span arial="" style="font-family:"><span style="letter-spacing:-.2pt">Materials and methods:</span></span></span></b> <span style="font-size:10.0pt">In this experimental study, 24 Wistar rats weighing 200-220 g were allocated into four groups (n = 6). To induce dependence, opium was dissolved in 1 mL of hot water and administered twice daily (morning and afternoon) for four weeks via oral gavage after cooling. At the end of the experiment, blood samples were collected for serum separation, and liver tissues were excised. Lipid profile parameters were measured using colorimetric methods. Hematoxylin and eosin staining was performed to evaluate histopathological changes in liver tissue. Data were analyzed using SPSS software and expressed as mean ± standard error of the mean (SEM).</span></span></span><br>
<span style="font-size:12pt"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:10.0pt"><span arial="" style="font-family:"><span style="letter-spacing:-.2pt">Results:</span></span></span></b><b> </b><span style="font-size:10.0pt">The results of this study demonstrated that opium significantly reduced serum levels of triglycerides (P= 0.0019), HDL-C (P= 0.0004), and VLDL-C (P= 0.0019) in the opium-dependent group compared with the healthy control group (P< 0.05). Histopathological analysis revealed that opium induced disorganization, deformation, and destruction of hepatocytes, along with lymphocytic infiltration in liver tissue.</span></span></span><br>
<span style="font-size:12pt"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:10.0pt"><span arial="" style="font-family:">Conclusion:</span></span></b> <span style="font-size:10.0pt">Although opium was associated with reductions in serum total cholesterol and triglycerides and may exhibit some metabolic effects, the histological findings of this study indicate that it exerts destructive effects on liver tissue.</span></span></span></span><br>
تریاک, لیپید, کبد, هیستولوژی
opium, lipid, liver, histology
42
51
http://jmums.mazums.ac.ir/browse.php?a_code=A-10-14068-3&slc_lang=fa&sid=1
Elaheh
Ebrahimi Bahar
الهه
ابراهیمی بهار
elaheh1ebrahimi@gmail.com