Journal of Mazandaran University of Medical Sciences
مجله دانشگاه علوم پزشکی مازندران
J Mazandaran Univ Med Sci
Medical Sciences
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اثرات پلاسمای غنی از پلاکت بر تظاهرات بالینی و التهابی در مدل تجربی کولیت اولسراتیو
Effects of Platelet-Rich Plasma on Clinical and Inflammatory Manifestations in an Experimental Model of Ulcerative Colitis
گوارش و کبد
Gastroenterology and Hepatology
پژوهشي-کامل
Research(Original)
<span style="font-size:12pt"><span style="line-height:89%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span lang="FA" style="line-height:89%"><span b="" koodak="" style="font-family:"><span style="color:black"><span style="letter-spacing:-.1pt">سابقه</span></span></span></span><span lang="AR-SA" style="line-height:89%"><span b="" koodak="" style="font-family:"><span style="color:black"><span style="letter-spacing:-.1pt"> و هدف:</span></span></span></span> <span b="" style="font-family:" zar=""><span style="letter-spacing:-.1pt">کولیت اولسراتیو یک بیماری التهابی روده است که عواملی از قبیل استعداد ژنتیکی، فاکتورهای محیطی</span></span><span b="" style="font-family:" zar=""> و عملکرد نامتناسب سیستم ایمنی </span><span lang="FA" style="line-height:89%"><span b="" style="font-family:" zar="">مخاطی در پاسخ به فلور میکروبی روده در این بیماری دخیل هستند. پلاسمای غنی از <span style="letter-spacing:-.2pt">پلاکت یکی از مشتقات حاصل از خون است که خاصیت ضد التهابی و بازسازیکننده سلولی دارد. مطالعه حاضر جهت بررسی</span> اثر ضد التهابی پلاسمای غنی از پلاکت بر تظاهرات بالینی و التهابی در مدل تجربی کولیت اولسراتیو صورت پذیرفت.<span style="color:#0e101a"></span></span></span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:89%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span lang="AR-SA" style="line-height:89%"><span b="" koodak="" style="font-family:"><span style="color:black"><span style="letter-spacing:-.1pt">مواد و روش</span></span></span></span><span lang="FA" style="line-height:89%"><span b="" style="font-family:" zar=""><span style="color:black"><span style="letter-spacing:-.1pt"></span></span></span></span><span lang="AR-SA" style="line-height:89%"><span b="" koodak="" style="font-family:"><span style="color:black"><span style="letter-spacing:-.1pt">ها:</span></span></span></span> <span lang="AR-SA" style="line-height:89%"><span b="" style="font-family:" zar="">در این مطالعه تجربی، از موش ها</span></span><span lang="FA" style="line-height:89%"><span b="" style="font-family:" zar="">ی نر </span></span><span lang="AR-SA" style="line-height:89%"><span b="" style="font-family:" zar="">نژاد </span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:89%">BALB/c</span></span><span lang="AR-SA" style="line-height:89%"><span b="" style="font-family:" zar=""> استفاده شد که به 4 گروه 10 تایی تقسیم شدند که شامل، گروه سالم (کنترل منفی)</span></span><span lang="AR-SA" style="line-height:89%"><span b="" style="font-family:" zar="">،</span></span><span lang="AR-SA" style="line-height:89%"><span b="" style="font-family:" zar=""> گروه کولیت بدون درمان</span></span> <span lang="AR-SA" style="line-height:89%"><span b="" style="font-family:" zar="">(کنترل مثبت)</span></span><span lang="AR-SA" style="line-height:89%"><span b="" style="font-family:" zar="">، </span></span><span lang="AR-SA" style="line-height:89%"><span b="" style="font-family:" zar="">گروه کولیت دریافتکننده </span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:89%">PRP</span></span><span lang="FA" style="line-height:89%"><span b="" style="font-family:" zar=""> و گروه کولیت دریافتکننده سولفاسالازین، است</span></span><span lang="AR-SA" style="line-height:89%"><span b="" style="font-family:" zar="">. </span></span><span lang="AR-SA" style="line-height:89%"><span b="" style="font-family:" zar="">کولیت با تزریق درون رکتومی </span></span><span lang="FA" style="line-height:89%"><span b="" style="font-family:" zar="">100 میکرولیتر </span></span><span lang="AR-SA" style="line-height:89%"><span b="" style="font-family:" zar="">اسید استیک 4 درصد در تمام حیوانات به جز گروه کنترل منفی ایجاد شد. برنامه درمانی پس از القای کولیت و ظهور علایم آغاز گردید. </span></span><span lang="FA" style="background:white"><span style="line-height:89%"><span b="" style="font-family:" zar="">موش</span></span></span><span b="" style="font-family:" zar=""><span style="letter-spacing:-.1pt"></span></span><span lang="FA" style="background:white"><span style="line-height:89%"><span b="" style="font-family:" zar="">ها 15 روز پس از آخرین تزریق آسان کشی شدند و </span></span></span><span lang="FA" style="line-height:89%"><span b="" style="font-family:" zar="">میزان معیار فعالیت بیماری و فاکتورهای التهابی بررسی گردید.<span style="color:black"></span></span></span></span></span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:89%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span lang="AR-SA" style="line-height:89%"><span b="" koodak="" style="font-family:"><span style="color:black"><span style="letter-spacing:-.2pt">یافته</span></span></span></span><span lang="FA" style="line-height:89%"><span b="" style="font-family:" zar=""><span style="color:black"><span style="letter-spacing:-.2pt"></span></span></span></span><span lang="AR-SA" style="line-height:89%"><span b="" koodak="" style="font-family:"><span style="color:black"><span style="letter-spacing:-.2pt">ها:</span></span></span></span> <span lang="FA" style="line-height:89%"><span b="" style="font-family:" zar="">نتایج نشان داد که معیار فعالیت بیماری، شدت التهاب و همچنین مقادیر </span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:89%">MPO</span></span><span lang="FA" style="line-height:89%"><span b="" style="font-family:" zar="">، </span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:89%">NO</span></span><span lang="FA" style="line-height:89%"><span b="" style="font-family:" zar="">، بیان و تولید سایتوکاین</span></span><span b="" style="font-family:" zar=""><span style="letter-spacing:-.1pt"></span></span><span lang="FA" style="line-height:89%"><span b="" style="font-family:" zar="">های </span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:89%">IL-1β</span></span><span lang="FA" style="line-height:89%"><span b="" style="font-family:" zar="">، </span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:89%">IL-6</span></span><span lang="FA" style="line-height:89%"><span b="" style="font-family:" zar=""> و </span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:89%">TNF-α</span></span><span lang="FA" style="line-height:89%"><span b="" style="font-family:" zar="">، بیان ژنهای التهابی </span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:89%">COX2</span></span><span lang="FA" style="line-height:89%"><span b="" style="font-family:" zar=""> و </span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:89%">iNOS</span></span><span lang="FA" style="line-height:89%"><span b="" style="font-family:" zar=""> در گروههای درمانی به نسبت گروه کولیت بدون درمان کاهش معناداری یافته است.<span style="color:black"></span></span></span></span></span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:89%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span lang="AR-SA" style="line-height:89%"><span b="" koodak="" style="font-family:"><span style="color:black"><span style="letter-spacing:-.2pt">استنتاج:</span></span></span></span> <span lang="FA" style="line-height:89%"><span b="" style="font-family:" zar="">نتایج نشان داد که پلاسمای غنی از پلاکت دارای اثرات ضد التهابی<span style="letter-spacing:-.1pt"> مطلوبی در بیماری کولیت اولسراتیو بوده</span> و بعد از ارزیابیهای تکمیلی می</span></span><span b="" style="font-family:" zar=""><span style="letter-spacing:-.1pt"></span></span><span lang="FA" style="line-height:89%"><span b="" style="font-family:" zar="">تواند به عنوان یک د</span></span><span b="" style="font-family:" zar="">رمان کمکی به همراه داروهای مورد مصرف در درمان بیماران مبتلا به کولیت اولسراتیو مورد استفاده قرار گیرد.</span></span></span></span></span></span></span></span>
<span style="font-size:12pt"><span style="line-height:130%"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:10.0pt"><span style="line-height:130%"><span arial="" style="font-family:"><span style="letter-spacing:-.2pt">Background and purpose:</span></span></span></span></b> <span style="font-size:10.0pt"><span style="line-height:130%">Ulcerative colitis is a kind of inflammatory bowel disease that involves factors such as genetic predisposition, environmental factors, and disproportionate function of the mucosal immune system in response to intestinal microbial flora. Platelet-rich plasma (PRP) is a blood derivative that has anti-inflammatory and cell-regenerating properties. This study aimed to investigate the effects of PRP on the clinical and inflammatory manifestations in an experimental model of ulcerative colitis.</span></span><span style="font-size:10.0pt"><span style="line-height:130%"></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:130%"><span style="text-autospace:none"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:10.0pt"><span style="line-height:130%"><span arial="" style="font-family:">Materials and methods:</span></span></span></b><b> </b><span style="font-size:10.0pt"><span style="line-height:130%">In this experiment, male BALB/c mice were segregated into four groups, each consisting of 10 mice: the healthy group (negative control), the colitis group without treatment (positive control), the colitis group receiving PRP, and colitis group receiving sulfasalazine. All animals, except for those in the negative control group, were subjected to colitis induction via intrarectal injection of 100 µl of 4% acetic acid. The treatment regimen commenced after the onset of UC symptoms. The mice were euthanasia after 15 days of the last injection, and the disease activity index, as well as the inflammatory factors, were assessed</span></span><span style="font-size:10.0pt"><span style="line-height:130%">.</span></span><span style="font-size:10.0pt"><span style="line-height:130%"></span></span></span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="line-height:130%"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:10.0pt"><span style="line-height:130%"><span arial="" style="font-family:">Results:</span></span></span></b><b> </b><span style="font-size:10.0pt"><span style="line-height:130%"><span style="font-family:" timesnewromanpsmt="">According to the results, the treatment groups showed a significant decrease in disease activity index, the severity of inflammation, the level of MPO, NO, and IL-1</span></span></span><span style="font-size:10.0pt"><span style="line-height:130%">β</span></span><span style="font-size:10.0pt"><span style="line-height:130%"><span style="font-family:" timesnewromanpsmt="">, IL-6, and TNF-α cytokines expression and production, as well as inflammatory genes COX2 and iNOS expression, compared to the positive control group</span></span></span><span style="font-size:10.0pt"><span style="line-height:130%">.</span></span><span lang="EN" style="font-size:10.0pt"><span style="line-height:130%"></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="line-height:130%"><span style="vertical-align:top"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:10.0pt"><span style="line-height:130%"><span arial="" style="font-family:">Conclusion:</span></span></span></b> <span style="font-size:10.0pt"><span style="line-height:130%">The results showed that PRP has favorable anti-inflammatory effects in ulcerative colitis and after additional evaluations, it can be used as an adjunctive treatment along with the drugs used in the treatment of ulcerative colitis patients.</span></span></span></span></span></span>
کولیت اولسراتیو, التهاب, پلاسمای غنی از پلاکت, سولفاسالازین
ulcerative colitis, inflammation, platelet-rich plasma, sulfasalazine
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http://jmums.mazums.ac.ir/browse.php?a_code=A-10-3860-6&slc_lang=fa&sid=1
Maryam
Ranjbar Bushehri
مریم
رنجبر بوشهری
maryamranjbar2017@gmail.com