Journal of Mazandaran University of Medical Sciences
مجله دانشگاه علوم پزشکی مازندران
J Mazandaran Univ Med Sci
Medical Sciences
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اثر تمرین هوازی مزمن بر سطح C3aR1 در بافت پانکراس رتهای دیابتی
The Effect of Chronic Aerobic Training on C3aR1 in the Pancreas of Diabetic Rats
فیزیولوژی جانوری
Animal physiology
پژوهشي-کامل
Research(Original)
<span style="line-height:2;"><span style="font-size:12pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><b><span lang="FA"><span style="font-family:"B Koodak""><span style="letter-spacing:-.2pt">سابقه و هدف:</span></span></span></b> <span lang="FA"><span style="font-family:"B Zar""><span style="color:black">دیابت نوع ۲، با التهاب مزمن و فعالسازی سیستم کمپلمان (اجزای کلیدی سیستم ایمنی ذاتی) همراه است که منجر به اختلال در عملکرد پانکراس میشود. اطلاعات در مورد تأثیر تمرین ورزشی بر بیان ژن و پروتئین گیرنده </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="color:black">C3aR1</span></span><span lang="FA"><span style="font-family:"B Zar""><span style="color:black"> در بافت پانکراس محدود است. از این رو این مطالعه با هدف بررسی تأثیر ۱۲ هفته تمرین هوازی مزمن بر بیان ژن و پروتئین </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="color:black">C3aR1</span></span><span lang="FA"><span style="font-family:"B Zar""><span style="color:black"> در بافت پانکراس موشهای دیابتی نوع ۲ و ارتباط آن با شاخصهای متابولیکی و بافت چربی قهوهای انجام شده است.</span></span></span><span lang="FA"><span style="font-family:"B Roya""><span style="letter-spacing:-.2pt"></span></span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><b><span lang="AR-SA"><span style="font-family:"B Koodak""><span style="letter-spacing:-.2pt">مواد و روش </span></span></span></b><b><span lang="FA"><span style="font-family:"B Koodak""><span style="letter-spacing:-.2pt"></span></span></span></b><b><span lang="AR-SA"><span style="font-family:"B Koodak""><span style="letter-spacing:-.2pt">ها:</span></span></span></b> <span lang="AR-SA"><span style="font-family:"B Zar""><span style="color:black"><span style="letter-spacing:-.1pt">در این مطالعه تجربی، </span></span></span></span><span lang="FA"><span style="font-family:"B Zar""><span style="color:black"><span style="letter-spacing:-.1pt">۳۶</span></span></span></span><span lang="AR-SA"><span style="font-family:"B Zar""><span style="color:black"><span style="letter-spacing:-.1pt"> سر موش نر نژاد ویستار پس از القای دیابت نوع </span></span></span></span><span lang="FA"><span style="font-family:"B Zar""><span style="color:black"><span style="letter-spacing:-.1pt">۲</span></span></span></span><span lang="AR-SA"><span style="font-family:"B Zar""><span style="color:black"><span style="letter-spacing:-.1pt">، با رژیم غذایی پرچرب به مدت دو هفته و تزریق استرپتوزوتوسین (</span></span></span></span><span lang="FA"><span style="font-family:"B Zar""><span style="color:black"><span style="letter-spacing:-.1pt">۳۰</span></span></span></span><span lang="AR-SA"><span style="font-family:"B Zar""><span style="color:black"><span style="letter-spacing:-.1pt"> میلیگرم بر کیلوگرم وزن بدن) به چهار گروه کنترل، دیابت، تمرین و دیابت+تمرین تقسیم شدند. تمرین به مدت </span></span></span></span><span lang="FA"><span style="font-family:"B Zar""><span style="color:black"><span style="letter-spacing:-.1pt">۱۲</span></span></span></span><span lang="AR-SA"><span style="font-family:"B Zar""><span style="color:black"><span style="letter-spacing:-.1pt"> هفته بر روی تردمیل انجام شد. بیان ژن</span></span></span></span><span lang="FA"><span style="font-family:"B Zar""><span style="color:black"></span></span></span><span lang="AR-SA"><span style="font-family:"B Zar""><span style="color:black"><span style="letter-spacing:-.1pt">های </span></span></span></span><i><span dir="LTR" style="font-size:10.0pt"><span style="color:black"><span style="letter-spacing:-.1pt">c3ar1</span></span></span></i><span lang="AR-SA"><span style="font-family:"B Zar""><span style="color:black"><span style="letter-spacing:-.1pt"> پانکراس و</span></span></span></span><i><span dir="LTR" style="font-size:10.0pt"><span style="color:black"><span style="letter-spacing:-.1pt">ucp1</span></span></span></i><span lang="AR-SA"><span style="font-family:"B Zar""><span style="color:black"><span style="letter-spacing:-.1pt"> بافت چربی قهوهای با روش</span></span></span></span> <span dir="LTR" style="font-size:10.0pt"><span style="color:black"><span style="letter-spacing:-.1pt">Real-Time PCR</span></span></span><span lang="AR-SA"><span style="font-family:"B Zar""><span style="color:black"><span style="letter-spacing:-.1pt"> و سطوح پروتئینی </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="color:black"><span style="letter-spacing:-.1pt">C3aR1</span></span></span><span lang="AR-SA"><span style="font-family:"B Zar""><span style="color:black"><span style="letter-spacing:-.1pt"> و</span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="color:black"><span style="letter-spacing:-.1pt">UCP1</span></span></span><span lang="AR-SA"><span style="font-family:"B Zar""><span style="color:black"><span style="letter-spacing:-.1pt"></span></span></span></span> <span lang="AR-SA"><span style="font-family:"B Zar""><span style="color:black"><span style="letter-spacing:-.1pt"></span></span></span></span><span lang="FA"><span style="font-family:"B Zar""><span style="color:black">با </span></span></span><span lang="AR-SA"><span style="font-family:"B Zar""><span style="color:black"><span style="letter-spacing:-.1pt">روش وسترن بلات و تغییرات مورفولوژیکی بافت چربی قهوهای با روشهای بافتشناسی و آنالیز مورفومتریک با نرمافزار </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="color:black"><span style="letter-spacing:-.1pt">ImageJ</span></span></span><span lang="AR-SA"><span style="font-family:"B Zar""><span style="color:black"><span style="letter-spacing:-.1pt"> اندازهگیری شد.</span></span></span></span><span lang="FA"><span style="font-family:"B Zar""><span style="letter-spacing:-.2pt"></span></span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><b><span lang="AR-SA"><span style="font-family:"B Koodak""><span style="letter-spacing:-.2pt">یافته</span></span></span></b><b><span lang="FA"><span style="font-family:"B Koodak""><span style="letter-spacing:-.2pt"></span></span></span></b><b><span lang="AR-SA"><span style="font-family:"B Koodak""><span style="letter-spacing:-.2pt">ها:</span></span></span></b> <span lang="FA"><span style="font-family:"B Zar""><span style="color:black">نتایج نشان داد که القای دیابت موجب افزایش بیان ژن و سطح پروتئین </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="color:black">C3aR1</span></span><span style="font-family:"B Zar""><span style="color:black"> و تمرین هوازی موجب کاهش معنیدار آن در بافت پانکراس میشود که با کاهش معنیدار سطوح گلوکز خون همراه بوده است. علاوه بر این، در بافت چربی قهوهای، تمرین موجب افزایش سطح پروتئین </span></span><span dir="LTR" style="font-size:10.0pt"><span style="color:black">UCP1</span></span><span style="font-family:"B Zar""><span style="color:black"> و افزایش تعداد میتوکندریها شده است.</span></span><span lang="AR-SA"><span style="font-family:"B Zar""><span style="letter-spacing:-.2pt"></span></span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><b><span lang="FA"><span style="font-family:"B Koodak""><span style="letter-spacing:-.1pt">استنتاج:</span></span></span></b><span lang="FA"><span style="font-family:"B Zar""> <span style="color:black">تمرین هوازی با کاهش سطح گیرنده التهابی </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="color:black">C3aR1</span></span><span lang="FA"><span style="font-family:"B Zar""><span style="color:black"> در پانکراس و بهبود بیوژنز میتوکندریایی در بافت چربی قهوهای، میتواند</span></span></span> <span lang="FA"><span style="font-family:"B Zar""><span style="color:black">بهعنوان یک راهکار غیر دارویی مؤثر برای کنترل التهاب و بهبود متابولیسم در دیابت نوع ۲، مورد استفاده قرار گیرد.</span></span></span></span></span></span></span></span>
<span style="line-height:2;"><span style="font-size:12pt"><span style="font-family:"Times New Roman","serif""><b><span style="font-size:10.0pt"><span style="font-family:"Arial","sans-serif""><span style="letter-spacing:-.1pt">Background and purpose:</span></span></span></b> <span style="font-size:10.0pt"><span style="letter-spacing:-.2pt">Type 2 diabetes is associated with chronic inflammation and activation of the complement system, a key component of innate immunity, which may impair pancreatic function. Limited evidence exists regarding the effects of exercise on gene and protein expression of the C3aR1 receptor in pancreatic tissue. This study aimed to investigate the effects of 12 weeks of chronic aerobic training on C3aR1 gene and protein expression in the pancreas of type 2 diabetic rats and its relationship with metabolic indices and brown adipose tissue.</span></span><span style="font-size:10.0pt"><span style="line-height:150%"></span></span></span></span><br>
<span style="font-size:12pt"><span style="font-family:"Times New Roman","serif""><b><span style="font-size:10.0pt"><span style="font-family:"Arial","sans-serif""><span style="letter-spacing:-.2pt">Materials and methods:</span></span></span></b> <span style="font-size:10.0pt"><span style="letter-spacing:-.2pt">In this experimental study, 36 male Wistar rats were induced with type 2 diabetes using a two-week high-fat diet followed by an intraperitoneal injection of streptozotocin (30 mg/kg). They were then assigned to four groups: Control, Diabetes, Training, and Diabetes+Training. The training protocol consisted of 12 weeks of treadmill running. Gene expression of pancreatic C3aR1 and brown adipose tissue UCP1 was measured using real-time PCR, while protein expression levels of C3aR1 and UCP1 were assessed by Western blot analysis. Brown adipose tissue morphology was evaluated using histological techniques, and morphometric analysis was performed using ImageJ software.</span></span></span></span><br>
<span style="font-size:12pt"><span style="font-family:"Times New Roman","serif""><b><span style="font-size:10.0pt"><span style="font-family:"Arial","sans-serif""><span style="letter-spacing:-.2pt">Results:</span></span></span></b><b> </b><span style="font-size:10.0pt"><span style="letter-spacing:-.2pt">Diabetes induction significantly increased C3aR1 gene and protein expression in the pancreas, whereas aerobic training markedly decreased these levels. These changes were accompanied by reduced blood glucose levels. In brown adipose tissue, exercise increased UCP1 protein expression and enhanced brown adipocyte cells and mitochondrial content.</span></span></span></span><br>
<span style="font-size:12pt"><span style="font-family:"Times New Roman","serif""><b><span style="font-size:10.0pt"><span style="font-family:"Arial","sans-serif"">Conclusion:</span></span></b> <span style="font-size:10.0pt"><span style="letter-spacing:-.2pt">Aerobic exercise reduces pancreatic C3aR1 expression and promotes mitochondrial biogenesis in brown adipose tissue. These adaptations suggest that exercise may serve as an effective non-pharmacological intervention to reduce inflammation and improve metabolic health in type 2 diabetes.</span></span></span></span></span>
C3aR1, پانکراس, تمرین هوازی, دیابت, متابولیسم
C3aR1, Aerobic exercise, Pancreas, Metabolism, Diabetes
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http://jmums.mazums.ac.ir/browse.php?a_code=A-10-19285-1&slc_lang=fa&sid=1
Pourya
Foroutan
پوریا
فروتن
pourya.foroutan.b@gmail.com