Journal of Mazandaran University of Medical Sciences
مجله دانشگاه علوم پزشکی مازندران
J Mazandaran Univ Med Sci
Medical Sciences
http://jmums.mazums.ac.ir
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2026
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مقایسه سطح مولیبدن ناخن و مالوندیآلدهید پلاسما در بیماران مبتلا به سرطان مری از منطقه پرخطر گنبد کاووس و افراد سالم از منطقه کمخطر ساری
Comparison of Nail Molybdenum and Plasma Malondialdehyde Levels Between Patients with Esophageal Cancer from the High-Risk Region of Gonbad-e Kavus and Healthy Individuals from the Low-Risk Region of Sari
بیوشیمی
Sports biomechanics
پژوهشي-کامل
Research(Original)
<span style="line-height:2;"><span style="direction:rtl"><span style="unicode-bidi:embed"><b><span lang="FA"><span style="font-family:"B Koodak""><span style="letter-spacing:-.2pt">سابقه و هدف:</span></span></span></b> <span lang="FA" style="font-family:"B Zar"">سرطان مری یکی از سرطان</span><span lang="AR-SA"><span style="font-family:"B Zar""></span></span><span lang="FA" style="font-family:"B Zar"">های شایع با نرخ بالای مرگ و میر در ایران است. استان گلستان و شهرستان گنبد کاووس از جمله کانون</span><span lang="AR-SA"><span style="font-family:"B Zar""></span></span><span lang="FA" style="font-family:"B Zar"">های جغرافیایی مورد توجه در این زمینه هستند. مولیبدن به عنوان یک عنصر ضروری در فعالیت آنزیم</span><span lang="AR-SA"><span style="font-family:"B Zar""></span></span><span lang="FA" style="font-family:"B Zar"">های بدن و مالون</span><span lang="AR-SA"><span style="font-family:"B Zar""></span></span><span lang="FA" style="font-family:"B Zar"">دی</span><span lang="AR-SA"><span style="font-family:"B Zar""></span></span><span lang="FA" style="font-family:"B Zar"">آلدهید به عنوان نشانگر استرس اکسیداتیو، می</span><span lang="AR-SA"><span style="font-family:"B Zar""></span></span><span lang="FA" style="font-family:"B Zar"">توانند نقش مهمی در مکانیسم</span><span lang="AR-SA"><span style="font-family:"B Zar""></span></span><span lang="FA" style="font-family:"B Zar"">های مرتبط با سرطان ایفا کنند. این مطالعه، با هدف بررسی ارتباط بین سطح مولیبدن ناخن و شاخص استرس اکسیداتیو (مالون</span><span lang="AR-SA"><span style="font-family:"B Zar""></span></span><span lang="FA" style="font-family:"B Zar"">دی</span><span lang="AR-SA"><span style="font-family:"B Zar""></span></span><span lang="FA" style="font-family:"B Zar"">آلدهید) در بیماران مبتلا به سرطان مری در مقایسه با افراد سالم، انجام پذیرفت.</span><span lang="FA"><span style="font-family:"B Roya""><span style="letter-spacing:-.2pt"></span></span></span></span></span><br>
<span style="direction:rtl"><span style="unicode-bidi:embed"><b><span lang="AR-SA"><span style="font-family:"B Koodak""><span style="letter-spacing:-.2pt">مواد و روش </span></span></span></b><b><span lang="FA"><span style="font-family:"B Koodak""><span style="letter-spacing:-.2pt"></span></span></span></b><b><span lang="AR-SA"><span style="font-family:"B Koodak""><span style="letter-spacing:-.2pt">ها:</span></span></span></b> <span lang="FA"><span style="font-family:"B Zar"">این مطالعه مورد-شاهدی، بر روی 30 بیمار مبتلا به سرطان مری از شهر گنبد کاووس و 30 فرد سالم از شهر ساری انجام شد. میزان مولیبدن در نمونه</span></span><span lang="AR-SA"><span style="font-family:"B Zar""></span></span><span lang="FA"><span style="font-family:"B Zar"">های ناخن دست و پای افراد، با روش طیف</span></span><span lang="AR-SA"><span style="font-family:"B Zar""></span></span><span lang="FA"><span style="font-family:"B Zar"">سنجی جرمی با پلاسمای جفت شده القایی (</span></span><span dir="LTR" style="font-size:10.0pt">ICP-MS</span><span lang="FA"><span style="font-family:"B Zar"">) اندازه</span></span><span lang="AR-SA"><span style="font-family:"B Zar""></span></span><span lang="FA"><span style="font-family:"B Zar"">گیری شد. هم</span></span><span lang="AR-SA"><span style="font-family:"B Zar""></span></span><span lang="FA"><span style="font-family:"B Zar"">چنین سطح مالون</span></span><span lang="AR-SA"><span style="font-family:"B Zar""></span></span><span lang="FA"><span style="font-family:"B Zar"">دی</span></span><span lang="AR-SA"><span style="font-family:"B Zar""></span></span><span lang="FA"><span style="font-family:"B Zar"">آلدهید نمونه</span></span><span lang="AR-SA"><span style="font-family:"B Zar""></span></span><span lang="FA"><span style="font-family:"B Zar"">های پلاسما با استفاده از روش اسپکتروفوتومتری (بر پایه واکنش با تیوباربیتوریک اسید) سنجیده شد. تحلیل</span></span><span lang="AR-SA"><span style="font-family:"B Zar""></span></span><span lang="FA"><span style="font-family:"B Zar"">های آماری با استفاده از نرم</span></span><span lang="AR-SA"><span style="font-family:"B Zar""></span></span><span lang="FA"><span style="font-family:"B Zar"">افزار </span></span><span dir="LTR" style="font-size:10.0pt">GraphPad Prism</span><span lang="FA"><span style="font-family:"B Zar""> انجام گرفت.<span style="letter-spacing:-.2pt"></span></span></span></span></span><br>
<span style="direction:rtl"><span style="unicode-bidi:embed"><b><span lang="AR-SA"><span style="font-family:"B Koodak""><span style="letter-spacing:-.2pt">یافته</span></span></span></b><b><span lang="FA"><span style="font-family:"B Koodak""><span style="letter-spacing:-.2pt"></span></span></span></b><b><span lang="AR-SA"><span style="font-family:"B Koodak""><span style="letter-spacing:-.2pt">ها:</span></span></span></b> <span lang="FA"><span style="font-family:"B Zar"">میانگین غلظت مولیبدن در نمونه</span></span><span lang="AR-SA"><span style="font-family:"B Zar""></span></span><span lang="FA"><span style="font-family:"B Zar"">های ناخن بیماران سرطانی نسبت به گروه کنترل، پایین</span></span><span lang="AR-SA"><span style="font-family:"B Zar""></span></span><span lang="FA"><span style="font-family:"B Zar"">تر بود، اما این اختلاف از نظر آماری معنی</span></span><span lang="AR-SA"><span style="font-family:"B Zar""></span></span><span lang="FA"><span style="font-family:"B Zar"">دار نبود (0/05</span></span><span lang="FA" style="font-size:10.0pt"><span style="font-family:"B Zar""><</span></span><span dir="LTR" style="font-size:10.0pt">P</span><span lang="FA"><span style="font-family:"B Zar"">). در مقابل سطح مالون دی</span></span><span lang="AR-SA"><span style="font-family:"B Zar""></span></span><span lang="FA"><span style="font-family:"B Zar"">آلدهید در گروه بیماران، به طور معنی</span></span><span lang="AR-SA"><span style="font-family:"B Zar""></span></span><span lang="FA"><span style="font-family:"B Zar"">داری بالاتر از گروه کنترل بود (0/05</span></span><span lang="FA" style="font-size:10.0pt"><span style="font-family:"B Zar"">></span></span><span dir="LTR" style="font-size:10.0pt">P</span><span lang="FA"><span style="font-family:"B Zar"">). آنالیز همبستگی، رابطه معنی</span></span><span lang="AR-SA"><span style="font-family:"B Zar""></span></span><span lang="FA"><span style="font-family:"B Zar"">داری بین این دو پارامتر در هیچ یک از دو گروه سالم و سرطانی نشان نداد (0/05</span></span><span lang="FA" style="font-size:10.0pt"><span style="font-family:"B Zar""><</span></span><span dir="LTR" style="font-size:10.0pt">P</span><span lang="FA"><span style="font-family:"B Zar"">).</span></span><span lang="AR-SA"><span style="font-family:"B Zar""><span style="letter-spacing:-.2pt"></span></span></span></span></span><br>
<span style="direction:rtl"><span style="unicode-bidi:embed"><b><span lang="FA"><span style="font-family:"B Koodak""><span style="letter-spacing:-.1pt">استنتاج:</span></span></span></b><span lang="FA"><span style="font-family:"B Zar""> شواهدی مبنی بر کاهش مولیبدن ناخن در بیماران مبتلا به سرطان مری نسبت به افراد سالم یافت نشد، اما افزایش </span></span><span dir="LTR" style="font-size:10.0pt">MDA</span><span lang="FA"><span style="font-family:"B Zar""> همسو با فرضیه نقش استرس اکسیداتیو است. انجام مطالعات آینده با حجم نمونه بزرگ</span></span><span lang="AR-SA"><span style="font-family:"B Zar""></span></span><span lang="FA"><span style="font-family:"B Zar"">تر، در قالب کوهورت آینده</span></span><span lang="AR-SA"><span style="font-family:"B Zar""></span></span><span lang="FA"><span style="font-family:"B Zar"">نگر و با در نظر گرفتن نسبت</span></span><span lang="AR-SA"><span style="font-family:"B Zar""></span></span><span lang="FA"><span style="font-family:"B Zar"">های عناصر کمیاب دیگر توصیه می</span></span><span lang="AR-SA"><span style="font-family:"B Zar""></span></span><span lang="FA"><span style="font-family:"B Zar"">شود.</span></span><span lang="AR-SA"><span style="font-family:"B Zar""></span></span></span></span><br>
<span style="direction:rtl"><span style="unicode-bidi:embed"><span lang="FA" style="font-size:6.0pt"><span style="font-family:"B Zar""></span></span></span></span></span><br>
<span style="line-height:2;"><b><span style="font-size:10.0pt"><span style="font-family:"Arial",sans-serif"><span style="letter-spacing:-.3pt">Background and purpose:</span></span></span></b><span style="font-size:10.0pt"><span style="letter-spacing:-.3pt"> Esophageal cancer is a prevalent malignancy with a high mortality rate in Iran. Golestan Province, particularly Gonbad-e Kavus, is considered one of the major geographic hotspots for this disease. Molybdenum, an essential trace element involved in the activity of several enzymes, and malondialdehyde (MDA), a biomarker of oxidative stress, may play important roles in cancer-related biological mechanisms. This study aimed to investigate the relationship between nail molybdenum levels and oxidative stress, as measured by malondialdehyde levels, in patients with esophageal cancer compared with healthy individuals.</span><span style="letter-spacing:-.3pt"></span></span><br>
<b><span style="font-size:10.0pt"><span style="font-family:"Arial",sans-serif"><span style="letter-spacing:-.2pt">Materials and methods:</span></span></span></b><span style="font-size:10.0pt"><span style="letter-spacing:-.2pt"> This case-control study was conducted among 30 patients with esophageal cancer from Gonbad-e Kavus and 30 healthy individuals from Sari, Iran. The molybdenum content of fingernail and toenail samples was measured using inductively coupled plasma mass spectrometry (ICP-MS). Additionally, plasma malondialdehyde levels were assessed using a spectrophotometric method based on the reaction between malondialdehyde and thiobarbituric acid. Statistical analyses were performed using GraphPad Prism software.</span><span style="letter-spacing:-.2pt"></span></span><br>
<b><span style="font-size:10.0pt"><span style="font-family:"Arial",sans-serif"><span style="letter-spacing:-.2pt">Results:</span></span></span></b><b> </b><span style="font-size:10.0pt">The mean concentration of molybdenum in the nail samples of cancer patients was lower than that of the control group; however, this difference was not statistically significant (P> 0.05). In contrast, malondialdehyde levels in the patient group were significantly higher than those in the control group (P< 0.05). Correlation analysis revealed no significant association between these two parameters in either the healthy or patient groups (P> 0.05).</span><br>
<b><span style="font-size:10.0pt"><span style="font-family:"Arial",sans-serif">Conclusion:</span></span></b> <span style="font-size:10.0pt">This study found no evidence of significantly reduced nail molybdenum concentrations in patients with esophageal cancer compared with healthy individuals. However, the elevated MDA levels observed among patients support the hypothesis that oxidative stress may contribute to the pathogenesis of esophageal cancer. Future studies with larger sample sizes, preferably using prospective cohort designs and incorporating assessments of additional trace elements and their interactions, are recommended.</span></span><br>
سرطان مری, مولیبدن, مالوندیآلدهید, استرس اکسیداتیو, ناخن
Esophageal cancer, molybdenum, malondialdehyde, oxidative stress, nail
110
119
http://jmums.mazums.ac.ir/browse.php?a_code=A-11-1703-6&slc_lang=fa&sid=1
Fateme
Moslemi
فاطمه
مسلمی
Fmoslemi80@gmail.com