fa بررسی سم آفلاتوکسین M1 در شیر گاومیش با استفاده از روش ایمنواسی بهداشت حرفه ای Health Professiona پژوهشي-کامل Research(Original) <p dir="RTL">سابقه و هدف: مایکوتوکسین‌ها گروهی از ترکیبات سمی طبیعی بوده که توسط گونه‌های متعددی از قارچ‌ها تولید می‌شوند که آفلاتوکسین‌ها مهم‌ترین آن‌ها است.آفلاتوکسین‌های <span dir="LTR">M₁</span><strong>_و</strong><span dir="LTR">M₂</span> مشتقاتی از آفلاتوکسین‌های <span dir="LTR">B₁</span> و<span dir="LTR">B₂</span> هستند که در مقابل تغییرات حرارتی از قبیل پاستوریزه کردن، استریلیزاسیون و اتوکلاو کردن مقاومت نشان داده در نتیجه&nbsp; غلظت آن‌ها کاهش پیدا نمی‌کند لذا توسط شیر و محصولات لبنی به انسان منتقل شده و سلامت انسان و جامعه را به خطر می‌اندازد. هدف از انجام این مطالعه بررسی آفلاتوکسین<span dir="LTR">M₁</span> در شیر گاومیش‌های دو شهرستان اهواز و کارون با استفاده از روش الایزا بوده است.</p> <p dir="RTL">مواد و روش‌ها: در این مطالعه توصیفی- تحلیلی 60 نمونه شیر به‌طور اتفاقی از 2دامداری که بیش‌ترین راس را در دو منطقه داشتند و در3 ماه از فصل پاییز(مهر، آبان، آذر) جمع‌آوری شد و روش الایزا برای تعیین آفلاتوکسین موجود در &nbsp;نمونه‌های شیر گاومیش استفاده شد.</p> <p dir="RTL">یافته‌ها: میانگین غلظت سم آفلاتوکسین <span dir="LTR">M₁</span> درکل نمونه‌های دو منطقه نامبرده طی سه ماه متوالیاز حد مجاز استاندارد ایران، اتحادیه اروپا(کدکس)و سازمان غذا بیش‌تر(91/155 <span dir="LTR">ng/l</span>) بود.</p> <p dir="RTL">استنتاج: طبق بررسی‌های انجام شده در مطالعه حاضر رابطه تنگاتنگی بین نوع جیره غذایی و روش نگهداری خوراک دام با میزان سم در شیر وجود داشت.</p> <p><strong>Background and purpose:</strong> Mycotoxins are a group of toxic compounds produced by several species of fungi and aflatoxins are the most important toxins. M1 and M2 which are derivatives of aflatoxin B1 and B2 are resistant against thermal changes, such as pasteurization, sterilization and autoclave and their concentration will not decrease. They are transmitted to humans by milk and milk products and threaten the health of human and society. The aim of this study was to determine aflatoxin M1 in buffaloes milk in Ahvaz and Karoon, Iran using ELIZA method.</p> <p><strong>Materials and methods:</strong> In a cross-sectional study, 60 milk samples were randomly collected from two farms with large number of buffalos, in autumn. ELISA method was used to evaluate of aflatoxins the samples.</p> <p><strong>Results:</strong> The average level of aflatoxin M1 in two selected locations was (155.91 ng/l) which was more than standard level in Iran and the Codex Alimentarius.</p> <p><strong>Conclusion:</strong> According to this research, there was a strong relationship between presence of toxin in milk with the animals&rsquo; diet and method of storing their foods.<strong>Background and purpose:</strong> Mycotoxins are a group of toxic compounds produced by several species of fungi and aflatoxins are the most important toxins. M1 and M2 which are derivatives of aflatoxin B1 and B2 are resistant against thermal changes, such as pasteurization, sterilization and autoclave and their concentration will not decrease. They are transmitted to humans by milk and milk products and threaten the health of human and society. The aim of this study was to determine aflatoxin M1 in buffaloes milk in Ahvaz and Karoon, Iran using ELIZA method.</p> <p><strong>Materials and methods:</strong> In a cross-sectional study, 60 milk samples were randomly collected from two farms with large number of buffalos, in autumn. ELISA method was used to evaluate of aflatoxins the samples.</p> <p><strong>Results:</strong> The average level of aflatoxin M1 in two selected locations was (155.91 ng/l) which was more than standard level in Iran and the Codex Alimentarius.</p> <p><strong>Conclusion:</strong> According to this research, there was a strong relationship between presence of toxin in milk with the animals&rsquo; diet and method of storing their foods.</p> <p><strong>Background and purpose:</strong> Mycotoxins are a group of toxic compounds produced by several species of fungi and aflatoxins are the most important toxins. M1 and M2 which are derivatives of aflatoxin B1 and B2 are resistant against thermal changes, such as pasteurization, sterilization and autoclave and their concentration will not decrease. They are transmitted to humans by milk and milk products and threaten the health of human and society. The aim of this study was to determine aflatoxin M1 in buffaloes milk in Ahvaz and Karoon, Iran using ELIZA method.</p> <p><strong>Materials and methods:</strong> In a cross-sectional study, 60 milk samples were randomly collected from two farms with large number of buffalos, in autumn. ELISA method was used to evaluate of aflatoxins the samples.</p> <p><strong>Results:</strong> The average level of aflatoxin M1 in two selected locations was (155.91 ng/l) which was more than standard level in Iran and the Codex Alimentarius.</p> <p><strong>Conclusion:</strong> According to this research, there was a strong relationship between presence of toxin in milk with the animals&rsquo; diet and method of storing their foods.<strong>Background and purpose:</strong> Mycotoxins are a group of toxic compounds produced by several species of fungi and aflatoxins are the most important toxins. M1 and M2 which are derivatives of aflatoxin B1 and B2 are resistant against thermal changes, such as pasteurization, sterilization and autoclave and their concentration will not decrease. They are transmitted to humans by milk and milk products and threaten the health of human and society. The aim of this study was to determine aflatoxin M1 in buffaloes milk in Ahvaz and Karoon, Iran using ELIZA method.</p> <p><strong>Materials and methods:</strong> In a cross-sectional study, 60 milk samples were randomly collected from two farms with large number of buffalos, in autumn. ELISA method was used to evaluate of aflatoxins the samples.</p> <p><strong>Results:</strong> The average level of aflatoxin M1 in two selected locations was (155.91 ng/l) which was more than standard level in Iran and the Codex Alimentarius.</p> <p><strong>Conclusion:</strong> According to this research, there was a strong relationship between presence of toxin in milk with the animals&rsquo; diet and method of storing their foods.</p> <p><strong>Background and purpose:</strong> Mycotoxins are a group of toxic compounds produced by several species of fungi and aflatoxins are the most important toxins. M1 and M2 which are derivatives of aflatoxin B1 and B2 are resistant against thermal changes, such as pasteurization, sterilization and autoclave and their concentration will not decrease. They are transmitted to humans by milk and milk products and threaten the health of human and society. The aim of this study was to determine aflatoxin M1 in buffaloes milk in Ahvaz and Karoon, Iran using ELIZA method.</p> <p><strong>Materials and methods:</strong> In a cross-sectional study, 60 milk samples were randomly collected from two farms with large number of buffalos, in autumn. ELISA method was used to evaluate of aflatoxins the samples.</p> <p><strong>Results:</strong> The average level of aflatoxin M1 in two selected locations was (155.91 ng/l) which was more than standard level in Iran and the Codex Alimentarius.</p> <p><strong>Conclusion:</strong> According to this research, there was a strong relationship between presence of toxin in milk with the animals&rsquo; diet and method of storing their foods.<strong>Background and purpose:</strong> Mycotoxins are a group of toxic compounds produced by several species of fungi and aflatoxins are the most important toxins. M1 and M2 which are derivatives of aflatoxin B1 and B2 are resistant against thermal changes, such as pasteurization, sterilization and autoclave and their concentration will not decrease. They are transmitted to humans by milk and milk products and threaten the health of human and society. The aim of this study was to determine aflatoxin M1 in buffaloes milk in Ahvaz and Karoon, Iran using ELIZA method.</p> <p><strong>Materials and methods:</strong> In a cross-sectional study, 60 milk samples were randomly collected from two farms with large number of buffalos, in autumn. ELISA method was used to evaluate of aflatoxins the samples.</p> <p><strong>Results:</strong> The average level of aflatoxin M1 in two selected locations was (155.91 ng/l) which was more than standard level in Iran and the Codex Alimentarius.</p> <p><strong>Conclusion:</strong> According to this research, there was a strong relationship between presence of toxin in milk with the animals&rsquo; diet and method of storing their foods.</p> <p><strong>Background and purpose:</strong> Mycotoxins are a group of toxic compounds produced by several species of fungi and aflatoxins are the most important toxins. M1 and M2 which are derivatives of aflatoxin B1 and B2 are resistant against thermal changes, such as pasteurization, sterilization and autoclave and their concentration will not decrease. They are transmitted to humans by milk and milk products and threaten the health of human and society. The aim of this study was to determine aflatoxin M1 in buffaloes milk in Ahvaz and Karoon, Iran using ELIZA method.</p> <p><strong>Materials and methods:</strong> In a cross-sectional study, 60 milk samples were randomly collected from two farms with large number of buffalos, in autumn. ELISA method was used to evaluate of aflatoxins the samples.</p> <p><strong>Results:</strong> The average level of aflatoxin M1 in two selected locations was (155.91 ng/l) which was more than standard level in Iran and the Codex Alimentarius.</p> <p><strong>Conclusion:</strong> According to this research, there was a strong relationship between presence of toxin in milk with the animals&rsquo; diet and method of storing their foods.<strong>Background and purpose:</strong> Mycotoxins are a group of toxic compounds produced by several species of fungi and aflatoxins are the most important toxins. M1 and M2 which are derivatives of aflatoxin B1 and B2 are resistant against thermal changes, such as pasteurization, sterilization and autoclave and their concentration will not decrease. They are transmitted to humans by milk and milk products and threaten the health of human and society. The aim of this study was to determine aflatoxin M1 in buffaloes milk in Ahvaz and Karoon, Iran using ELIZA method.</p> <p><strong>Materials and methods:</strong> In a cross-sectional study, 60 milk samples were randomly collected from two farms with large number of buffalos, in autumn. ELISA method was used to evaluate of aflatoxins the samples.</p> <p><strong>Results:</strong> The average level of aflatoxin M1 in two selected locations was (155.91 ng/l) which was more than standard level in Iran and the Codex Alimentarius.</p> <p><strong>Conclusion:</strong> According to this research, there was a strong relationship between presence of toxin in milk with the animals&rsquo; diet and method of storing their foods.</p> <p><strong>Background and purpose:</strong> Mycotoxins are a group of toxic compounds produced by several species of fungi and aflatoxins are the most important toxins. M1 and M2 which are derivatives of aflatoxin B1 and B2 are resistant against thermal changes, such as pasteurization, sterilization and autoclave and their concentration will not decrease. They are transmitted to humans by milk and milk products and threaten the health of human and society. The aim of this study was to determine aflatoxin M1 in buffaloes milk in Ahvaz and Karoon, Iran using ELIZA method.</p> <p><strong>Materials and methods:</strong> In a cross-sectional study, 60 milk samples were randomly collected from two farms with large number of buffalos, in autumn. ELISA method was used to evaluate of aflatoxins the samples.</p> <p><strong>Results:</strong> The average level of aflatoxin M1 in two selected locations was (155.91 ng/l) which was more than standard level in Iran and the Codex Alimentarius.</p> <p><strong>Conclusion:</strong> According to this research, there was a strong relationship between presence of toxin in milk with the animals&rsquo; diet and method of storing their foods.</p> <p><strong>Background and purpose:</strong> Mycotoxins are a group of toxic compounds produced by several species of fungi and aflatoxins are the most important toxins. M1 and M2 which are derivatives of aflatoxin B1 and B2 are resistant against thermal changes, such as pasteurization, sterilization and autoclave and their concentration will not decrease. They are transmitted to humans by milk and milk products and threaten the health of human and society. The aim of this study was to determine aflatoxin M1 in buffaloes milk in Ahvaz and Karoon, Iran using ELIZA method.</p> <p><strong>Materials and methods:</strong> In a cross-sectional study, 60 milk samples were randomly collected from two farms with large number of buffalos, in autumn. ELISA method was used to evaluate of aflatoxins the samples.</p> <p><strong>Results:</strong> The average level of aflatoxin M1 in two selected locations was (155.91 ng/l) which was more than standard level in Iran and the Codex Alimentarius.</p> <p><strong>Conclusion:</strong> According to this research, there was a strong relationship between presence of toxin in milk with the animals&rsquo; diet and method of storing their foods.</p> آفلاتوکسین M1, شیر, ایمنی غذا, الایزا aflatoxin M1, milk, food safety, ELISA 248 256 http://jmums.mazums.ac.ir/browse.php?a_code=A-10-29-473&slc_lang=fa&sid=1 Huria Ghariby حوریا غریبی afshin_ir@yahoo.com No , Afshin Takdastan افشین تکدستان Yes Abdol Kazem Neisi عبدالکاظم نیسی No Hamideh Rezazadeh حمیده رضازاده No Hasan Kuhpaee حسن کوهپایی No دانشجوی ارشد ایمنی و محیط زیست، دانشکده سلامت ، دانشگاه علوم پزشکی شهید بهشتی، تهران، ایران