Volume 12, Issue 36 (Sep 2002)
J Mazandaran Univ Med Sci 2002, 12(36): 16-23 |
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Ësmaeeli M, Moradi S. Comparison of three diagnostic tests: histology, serology and Rapid urease test (RUT) in identification of Helicobacter pylori infection in children . J Mazandaran Univ Med Sci 2002; 12 (36) :16-23
URL: http://jmums.mazums.ac.ir/article-1-322-en.html
URL: http://jmums.mazums.ac.ir/article-1-322-en.html
Abstract: (20706 Views)
Background and purpose : Helicobacter pylori (H.pylori) is responsible for causing many diseases in children. Ïn the recent years different methods have been used to identify this infection. The aim of this study is to compare the results of above mentioned methods in identification of H.pylori in children.
Materials and methods : This prospective study was done on 50 infants aged 3 to 14 years having various complaints under went upper gastrointestinal endoscopy in Âmir kolah pediatrics hospital in the year 2000. Biopsy specimen was obtained for microbiological examination (Giemsa staining) and urease test in BÏRD medium. Blood was collected from the patients for serologic test (using RÂDÏM kit). The standard ratio for identification of H.pylori infection in this study was indicated as at least two positive tests out of three tests.
Results : Ôut of 50 infants under investigation, 19 (38%) were infected with H.pylori. Frequency distribution of this infection in boys was 45% and in girls 96.3% (P>0.05). Ïn this study the presence of ÏgG against H.pylori was determined by ËLÏSÂ with cut off=23 ur/ml. The sensitivity and specificity for ËLÏSÂ, histology and urease test was as follow: 89.4% and 96.7%, 89.4% and 96.7, 89.4% and 100% respectively.
Çonclusion : Ït is concluded from this study that, through standardization of ËLÏSÂ, the above mentioned three methods of the tests have the same sensitivity property. Though rapid urease test with higher specificity is more reliable method, but without standardization of ËLÏSÂ in infants the sensitivity and specificity would be lower.
Materials and methods : This prospective study was done on 50 infants aged 3 to 14 years having various complaints under went upper gastrointestinal endoscopy in Âmir kolah pediatrics hospital in the year 2000. Biopsy specimen was obtained for microbiological examination (Giemsa staining) and urease test in BÏRD medium. Blood was collected from the patients for serologic test (using RÂDÏM kit). The standard ratio for identification of H.pylori infection in this study was indicated as at least two positive tests out of three tests.
Results : Ôut of 50 infants under investigation, 19 (38%) were infected with H.pylori. Frequency distribution of this infection in boys was 45% and in girls 96.3% (P>0.05). Ïn this study the presence of ÏgG against H.pylori was determined by ËLÏSÂ with cut off=23 ur/ml. The sensitivity and specificity for ËLÏSÂ, histology and urease test was as follow: 89.4% and 96.7%, 89.4% and 96.7, 89.4% and 100% respectively.
Çonclusion : Ït is concluded from this study that, through standardization of ËLÏSÂ, the above mentioned three methods of the tests have the same sensitivity property. Though rapid urease test with higher specificity is more reliable method, but without standardization of ËLÏSÂ in infants the sensitivity and specificity would be lower.
Type of Study: Research(Original) |
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