Volume 36, Issue 263 (11-2026)
J Mazandaran Univ Med Sci 2026, 36(263): 50-62 |
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Rezaei-Hachesu V, Haddadzade Niri H, Farahani S, Monazzam Esmaeelpour M R. Hearing Impairment and Oxidative Stress FollowingRepeated Violet Noise Exposure: Protective Effects of Nigella sativa Oil in Rats. , J Mazandaran Univ Med Sci. 2026; 36 (263) :50-62
URL: http://jmums.mazums.ac.ir/article-1-23265-en.html
URL: http://jmums.mazums.ac.ir/article-1-23265-en.html
Vida Rezaei-Hachesu 
, Hassan Haddadzade Niri , Saeid Farahani , Mohammad Reza Monazzam Esmaeelpour *
, Hassan Haddadzade Niri , Saeid Farahani , Mohammad Reza Monazzam Esmaeelpour *
Abstract: (115 Views)
Background and purpose: Oxidative stress is a key mechanism underlying noise-induced hearing loss, and antioxidant compounds may help attenuate noise-induced auditory damage. This study investigated the protective effects of Nigella sativa oil on hearing impairment and changes in oxidative stress and antioxidant status following repeated violet noise exposure.
Materials and methods: In this experimental study, 36 adult male rats were randomly assigned to four groups: control, Nigella sativa oil, violet noise, and violet noise + Nigella sativa oil. The noise-exposed groups were subjected to violet noise at 110 dB SPL for 4 h/day for 14 days. Nigella sativa oil was administered by gavage at a dose of 2 mL/kg, 1 h before noise exposure. Auditory brainstem response (ABR) thresholds were measured using click and tone-burst stimuli at 4, 8, 12, 14 and 16 kHz at baseline and on days 7, 14 and 21. Serum levels of malondialdehyde (MDA), superoxide dismutase (SOD) activity, and total antioxidant capacity (TAC) were also assessed at these time points. Data were analysed using one-way and repeated-measures analysis of variance (ANOVA).
Results: Baseline ABR thresholds did not differ significantly among the groups. In the violet-noise group, ABR thresholds increased at all tested frequencies on days 7 and 14 relative to baseline (P < 0.05). Compared with the violet-noise group, the violet-noise + oil group showed significantly lower ABR thresholds at 14 and 16 kHz on day 7, at 12, 14 and 16 kHz on day 14, and at 14 kHz on day 21 (P < 0.05). The violet-noise group also exhibited higher serum MDA levels and lower SOD activity and TAC than the control and Nigella sativa oil groups. Compared with the violet-noise group, the violet-noise + oil group showed lower MDA levels on days 7 and 21, higher SOD activity at all three time points, and higher TAC on days 14 and 21 (P < 0.05).
Conclusion: Preventive administration of Nigella sativa oil attenuated hearing impairment following violet-noise exposure, particularly at higher frequencies, and was associated with improvements in serum oxidative stress and antioxidant markers. These findings suggest that Nigella sativa oil may have a protective effect against noise-induced auditory damage.
Materials and methods: In this experimental study, 36 adult male rats were randomly assigned to four groups: control, Nigella sativa oil, violet noise, and violet noise + Nigella sativa oil. The noise-exposed groups were subjected to violet noise at 110 dB SPL for 4 h/day for 14 days. Nigella sativa oil was administered by gavage at a dose of 2 mL/kg, 1 h before noise exposure. Auditory brainstem response (ABR) thresholds were measured using click and tone-burst stimuli at 4, 8, 12, 14 and 16 kHz at baseline and on days 7, 14 and 21. Serum levels of malondialdehyde (MDA), superoxide dismutase (SOD) activity, and total antioxidant capacity (TAC) were also assessed at these time points. Data were analysed using one-way and repeated-measures analysis of variance (ANOVA).
Results: Baseline ABR thresholds did not differ significantly among the groups. In the violet-noise group, ABR thresholds increased at all tested frequencies on days 7 and 14 relative to baseline (P < 0.05). Compared with the violet-noise group, the violet-noise + oil group showed significantly lower ABR thresholds at 14 and 16 kHz on day 7, at 12, 14 and 16 kHz on day 14, and at 14 kHz on day 21 (P < 0.05). The violet-noise group also exhibited higher serum MDA levels and lower SOD activity and TAC than the control and Nigella sativa oil groups. Compared with the violet-noise group, the violet-noise + oil group showed lower MDA levels on days 7 and 21, higher SOD activity at all three time points, and higher TAC on days 14 and 21 (P < 0.05).
Conclusion: Preventive administration of Nigella sativa oil attenuated hearing impairment following violet-noise exposure, particularly at higher frequencies, and was associated with improvements in serum oxidative stress and antioxidant markers. These findings suggest that Nigella sativa oil may have a protective effect against noise-induced auditory damage.
Type of Study: Research(Original) |
Subject:
Health Professiona
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