Volume 23, Issue 104 (9-2013)                   J Mazandaran Univ Med Sci 2013, 23(104): 2-10 | Back to browse issues page

XML Persian Abstract Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Yari K, Fatemi S S, Tavallaei M, Moradi M T. A Quantitative Comparison of Recombinant Hc Domain of Clostridium Botulinum Neurotoxin A in Escherichia Coli in Batch and Fed-Batch Cultivations. J Mazandaran Univ Med Sci. 2013; 23 (104) :2-10
URL: http://jmums.mazums.ac.ir/article-1-2618-en.html
Abstract:   (7430 Views)
Background and purpose: The 50 KDa protein (50 µg) in carboxylic domain of the neurotoxin heavy chain (BoNT/A-Hc) recognizes surface receptors on target neurons and this fragment contains the principle protective antigenic determinants. Recently, this fragment has been used as a recombinant vaccine candidate for botulism. The study aimed to compare the evaluation of BoNT/A-Hc production in fed-batch (high cell density cultivation) and batch cultivation of recombinant Escherichia coli (E. coli.). Materials and methods: In this research, growth of recombinant E. coli in batch culture was studied. In order to maximize protein expression, induction time and Isopropyl β-D-1-thiogalactopyranoside (IPTG) inducer concentration were optimized by the Taguchi statistical method. Then, fed-batch culture was applied to achieve high cell density cultivation. Finally, the recombinant protein expression level was determined. Results: In optimized conditions, 62 and 486 mg/l of soluble recombinant BoNT/A-Hc were produced in batch and fed-batch cultivation. Conclusion: According to the results, high cell density in fed-batch cultivation is a very effective for improve of recombinant proteins productivity.
Full-Text [PDF 309 kb]   (1990 Downloads)    
Type of Study: Research(Original) |

Add your comments about this article : Your username or Email:

Send email to the article author

© 2021 All Rights Reserved | Journal of Mazandaran University of Medical Sciences

Designed & Developed by : Yektaweb